BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146758.6 + phase: 0 /pseudo
(515 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC213164 138 7e-33
BE329487 weakly similar to PIR|T14619|T146 reverse transcriptase... 127 1e-29
TC211561 weakly similar to UP|O22675 (O22675) Reverse transcript... 112 3e-25
CA936755 98 2e-24
BI321779 108 4e-24
TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR ret... 60 2e-09
TC232603 weakly similar to UP|Q39482 (Q39482) Legumin (Fragment)... 48 4e-09
TC234193 56 4e-08
TC206673 50 2e-06
CO982349 49 7e-06
BI470652 similar to PIR|T17102|T171 RNA-directed DNA polymerase ... 47 3e-05
TC218847 45 1e-04
TC233199 35 2e-04
CA851997 36 0.036
BG405621 36 0.047
BI784748 35 0.081
CA851030 35 0.11
CD417230 weakly similar to GP|2244915|emb| reverse transcriptase... 32 0.52
TC221641 similar to UP|Q9LDX3 (Q9LDX3) T12C24.13 (F5O11.33), par... 32 0.68
TC225088 similar to UP|Q6WAT8 (Q6WAT8) Lipid tranfer protein II,... 30 2.0
>TC213164
Length = 446
Score = 138 bits (347), Expect = 7e-33
Identities = 65/135 (48%), Positives = 96/135 (70%)
Frame = +3
Query: 303 LIKPFSIDEVKAAVWDCDSYKSPGPDGINFGFIKEFWLDLKDEIFRFVSEFHRNGKLSKG 362
L+ F EV+A +WD + KSPGPDG+NF FIKEFW LK ++ RF+ EF+ NG K
Sbjct: 18 LVARFEKKEVRATMWDRGNAKSPGPDGLNFKFIKEFWDILKTDLLRFLDEFYANGVFLKR 197
Query: 363 INSTFIALIPKVDNPQRLNDFRPISLVGSLYKILAKLLANRLRVVIGSVISDAQSAFVKN 422
N+ F+ALIPKV +P LN+++PISL+G +YKI+AKLL+ R + V+ ++I + Q+ F++
Sbjct: 198 SNAFFLALIPKVHDP*SLNEYKPISLIGCIYKIVAKLLSGRHKKVLPTIIDERQTVFMEG 377
Query: 423 RQILDGILIANEAVD 437
R L ++IANE ++
Sbjct: 378 RHTLHNVVIANEIME 422
>BE329487 weakly similar to PIR|T14619|T146 reverse transcriptase - beet
retrotransposon (fragment), partial (15%)
Length = 376
Score = 127 bits (319), Expect = 1e-29
Identities = 58/88 (65%), Positives = 71/88 (79%)
Frame = -2
Query: 312 VKAAVWDCDSYKSPGPDGINFGFIKEFWLDLKDEIFRFVSEFHRNGKLSKGINSTFIALI 371
+K+AVW C S KSPGPDG+NF FIK FW LK +I RF++EFH NG KG N++FIALI
Sbjct: 375 IKSAVWQCGSVKSPGPDGLNFKFIKHFWERLKPDIIRFLNEFHANGIFPKGGNASFIALI 196
Query: 372 PKVDNPQRLNDFRPISLVGSLYKILAKL 399
PKV +PQ LNDFRPISL+G +YKI+AK+
Sbjct: 195 PKVKHPQALNDFRPISLIGCVYKIVAKI 112
>TC211561 weakly similar to UP|O22675 (O22675) Reverse transcriptase
(Fragment) , partial (59%)
Length = 1077
Score = 112 bits (281), Expect = 3e-25
Identities = 55/90 (61%), Positives = 66/90 (73%)
Frame = +1
Query: 426 LDGILIANEAVDEARKLKKDLLLFKVDFEKAYDSVDWGYLDSVMGRMSFPVLWRKWIKVC 485
L LIANE +DEA++ K L+FKVD+EKAYDSV W +L +M RM F W KWI+ C
Sbjct: 1 LHSALIANEVIDEAKRSNKSCLVFKVDYEKAYDSVSWNFLMYMMWRMDFSPKWIKWIEEC 180
Query: 486 VSTATASVLVNGSPTDEFPFRRGLRQGDPL 515
V +A+ SVLVNGSPT EF +RGLRQGDPL
Sbjct: 181 VKSASISVLVNGSPTAEFLPQRGLRQGDPL 270
>CA936755
Length = 424
Score = 98.2 bits (243), Expect(2) = 2e-24
Identities = 39/90 (43%), Positives = 57/90 (63%)
Frame = -2
Query: 71 FLLSEDWCLVWPNCLQIAQMRGLSDHCPLILSVAEENWGPRPSRMLKCWTDIPGYRQFVS 130
FL+SE W L WP+ Q R SDHCP+I+ + +WGP+P R++ W GY++ V
Sbjct: 294 FLVSEQWLLSWPDSSQYTLPRDFSDHCPIIMQTKKVDWGPKPFRVVDWWLHQKGYQRLVR 115
Query: 131 NKWKSFQVSGWGGYVLKEKFKLLKISLKEW 160
W + Q GWGG +LK K ++LK+S+K+W
Sbjct: 114 ETWSAEQQPGWGGILLKNKLRMLKLSIKQW 25
Score = 32.7 bits (73), Expect(2) = 2e-24
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -1
Query: 34 SHFNSFIDNNVLVDLPLGGRNFTWFKGDGKSMSRLDRF 71
S FN++I L ++ G +TW + + + SRLDRF
Sbjct: 406 SEFNTWISEMELQEIKCVGSTYTWIRPNARVKSRLDRF 293
>BI321779
Length = 421
Score = 108 bits (271), Expect = 4e-24
Identities = 54/115 (46%), Positives = 73/115 (62%)
Frame = +2
Query: 284 RPSLTNLQF*SLSVAEGVGLIKPFSIDEVKAAVWDCDSYKSPGPDGINFGFIKEFWLDLK 343
RP L ++F S S + LI+ F ++E+K + DC+S KSPGPDG + IK FW +K
Sbjct: 26 RPKLNGVEFLSFSNEDNAMLIQDFEVEEIKKVI*DCESSKSPGPDGYSLLLIKIFWEVIK 205
Query: 344 DEIFRFVSEFHRNGKLSKGINSTFIALIPKVDNPQRLNDFRPISLVGSLYKILAK 398
+E+ F EFH+ L +G N +FIALI K DNP + + PISLVG LYKI+ K
Sbjct: 206 EEVINFFKEFHKFDFLPRGTNRSFIALIAKCDNPLDIGQYCPISLVGCLYKIV*K 370
>TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR retroelement
reverse transcriptase, partial (7%)
Length = 956
Score = 60.1 bits (144), Expect = 2e-09
Identities = 28/69 (40%), Positives = 43/69 (61%)
Frame = -3
Query: 447 LLFKVDFEKAYDSVDWGYLDSVMGRMSFPVLWRKWIKVCVSTATASVLVNGSPTDEFPFR 506
L K+D KA+D++D +L SV+ + + + WI+V + +A S+ VNG P F +
Sbjct: 765 LALKIDIVKAFDTLDRDFLISVLNKFGYSHTFCNWIRVILLSAKLSISVNGEPVGFFSCQ 586
Query: 507 RGLRQGDPL 515
RG+RQGDPL
Sbjct: 585 RGVRQGDPL 559
>TC232603 weakly similar to UP|Q39482 (Q39482) Legumin (Fragment), partial
(4%)
Length = 1123
Score = 48.1 bits (113), Expect(2) = 4e-09
Identities = 22/47 (46%), Positives = 30/47 (63%)
Frame = +2
Query: 45 LVDLPLGGRNFTWFKGDGKSMSRLDRFLLSEDWCLVWPNCLQIAQMR 91
+V+L L GR + W+K D +MSRLDRFL+ E+ WPN I +R
Sbjct: 524 VVNLALVGRKYNWYKDDKTTMSRLDRFLVYEE*LTTWPNMTLILLLR 664
Score = 30.8 bits (68), Expect(2) = 4e-09
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = +1
Query: 3 GKNVCVCGDFNAIRSREERRSV 24
G+ C+ GDFNA+ S EER+ V
Sbjct: 421 GERWCIVGDFNAVCSEEERKGV 486
>TC234193
Length = 697
Score = 55.8 bits (133), Expect = 4e-08
Identities = 29/67 (43%), Positives = 43/67 (63%)
Frame = -3
Query: 328 DGINFGFIKEFWLDLKDEIFRFVSEFHRNGKLSKGINSTFIALIPKVDNPQRLNDFRPIS 387
+G NF FIK+ +K+++ R + EFH +G LS+G N++F+ P L DFRP++
Sbjct: 194 NGYNFNFIKKC*EVMKEDVVRAIQEFHSHGCLSRGTNASFL-----THPP*GLGDFRPVT 30
Query: 388 LVGSLYK 394
LVGSL K
Sbjct: 29 LVGSLCK 9
>TC206673
Length = 742
Score = 50.1 bits (118), Expect = 2e-06
Identities = 26/85 (30%), Positives = 47/85 (54%)
Frame = +3
Query: 172 ISDLKERLATLDEKGESMLLTDEECAEIHGVSSDILSLSRLNTSICWQQSRIQWLREGDA 231
I ++K++L +++ + L+ +E + + S S+ Q+SR +WL+EGD
Sbjct: 21 IHNIKKKLNEVEDLASNRSLSVDEIKAKKELQQQLWEASTAYESLLRQKSRDKWLKEGDN 200
Query: 232 NSKFFHSVLASRRRRNSLCTIVVDG 256
NS +FH+V+ RR N L I++ G
Sbjct: 201 NSAYFHTVINFRRHYNGLQGILIQG 275
>CO982349
Length = 795
Score = 48.5 bits (114), Expect = 7e-06
Identities = 21/35 (60%), Positives = 28/35 (80%)
Frame = +3
Query: 481 WIKVCVSTATASVLVNGSPTDEFPFRRGLRQGDPL 515
WI+ C+ +A+ S+LVN SP +EF +RGLRQGDPL
Sbjct: 69 WIRGCLHSASVSILVNESPINEFSPQRGLRQGDPL 173
>BI470652 similar to PIR|T17102|T171 RNA-directed DNA polymerase (EC
2.7.7.49) (clone AmLi2) - garden snapdragon (fragment),
partial (22%)
Length = 111
Score = 46.6 bits (109), Expect = 3e-05
Identities = 21/32 (65%), Positives = 26/32 (80%)
Frame = +1
Query: 483 KVCVSTATASVLVNGSPTDEFPFRRGLRQGDP 514
K C+S+A+ S+LVNGSPT+EF RGLRQG P
Sbjct: 1 KECLSSASISILVNGSPTEEFKPXRGLRQGGP 96
>TC218847
Length = 521
Score = 44.7 bits (104), Expect = 1e-04
Identities = 24/86 (27%), Positives = 48/86 (54%)
Frame = -3
Query: 210 SRLNTSICWQQSRIQWLREGDANSKFFHSVLASRRRRNSLCTIVVDGVVVEGVHPIREAV 269
++ + S+ Q++R +W+RE D NS +FH ++ R N++ +++ G VE + ++ AV
Sbjct: 273 AQAHESLLRQKARTKWIRERDCNSSYFHKLINYIHRTNAVNGVMIGGSWVEEPNRVK-AV 97
Query: 270 FCHFENHFRSVNVERPSLTNLQF*SL 295
F+ F + +RP + F S+
Sbjct: 96 RNFFQIRFSESDYDRP*INGANFRSI 19
>TC233199
Length = 1105
Score = 35.4 bits (80), Expect(2) = 2e-04
Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +2
Query: 7 CVCGDFNAIRSREERRSVSVGQVASDF-SHFNSFIDNNVLVDLPLGGRNFTWFK 59
C+ DFN +R + ER S + + FN +I + + D+P G +TW++
Sbjct: 293 CIVDDFNCVRRQSERVGASQREQGGGIMTEFNDWIVDLEVEDMPSVGHKYTWYR 454
Score = 27.7 bits (60), Expect(2) = 2e-04
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = +1
Query: 68 LDRFLLSEDWCLVWPNCLQIAQMRGLSDH 96
LDR L+S +W W +Q R SDH
Sbjct: 466 LDRVLVSVEWFSKWSGSIQFILDRNYSDH 552
>CA851997
Length = 636
Score = 36.2 bits (82), Expect = 0.036
Identities = 25/84 (29%), Positives = 40/84 (46%), Gaps = 2/84 (2%)
Frame = +1
Query: 327 PDGINFGFIKEFWLDLKDEIFRFVSEFHRNGKLSKGINSTFIALIPKVDNPQRLNDFRPI 386
PDG N GF FW +++F+ + G +N T IA+I ++ R
Sbjct: 316 PDGFNLGFYHRFWGMCGEDVFQACCMWLAEGAFPSSVNDTTIAIILEI**S*RYERS*TN 495
Query: 387 SLVGSLYKI--LAKLLANRLRVVI 408
L +KI L+++LA RL+ V+
Sbjct: 496 LLCNGGFKILFLSEVLAKRLKNVL 567
>BG405621
Length = 402
Score = 35.8 bits (81), Expect = 0.047
Identities = 16/22 (72%), Positives = 18/22 (81%), Gaps = 1/22 (4%)
Frame = +2
Query: 218 WQQ-SRIQWLREGDANSKFFHS 238
WQQ S+I WL+ GD NSKFFHS
Sbjct: 335 WQQRSKIFWLKYGDLNSKFFHS 400
>BI784748
Length = 259
Score = 35.0 bits (79), Expect = 0.081
Identities = 14/33 (42%), Positives = 25/33 (75%)
Frame = +2
Query: 416 QSAFVKNRQILDGILIANEAVDEARKLKKDLLL 448
QSAF++ R +L ++IANE +DEA++ +K ++
Sbjct: 14 QSAFIQGRHMLHSMMIANEVIDEAKRGQKPSIM 112
>CA851030
Length = 358
Score = 34.7 bits (78), Expect = 0.11
Identities = 17/39 (43%), Positives = 22/39 (55%), Gaps = 1/39 (2%)
Frame = +1
Query: 218 WQQ-SRIQWLREGDANSKFFHSVLASRRRRNSLCTIVVD 255
WQQ SR W +E D N++FFH R+ NS+ I D
Sbjct: 184 WQQRSRAIWFKERDKNTEFFHRKTLQRKASNSITKIQDD 300
>CD417230 weakly similar to GP|2244915|emb| reverse transcriptase like
protein {Arabidopsis thaliana}, partial (7%)
Length = 688
Score = 32.3 bits (72), Expect = 0.52
Identities = 15/31 (48%), Positives = 19/31 (60%)
Frame = -3
Query: 485 CVSTATASVLVNGSPTDEFPFRRGLRQGDPL 515
C+ST T S+L NG D F G+RQ DP+
Sbjct: 668 CMSTTTMSLLWNGEVLDHFSPNIGVRQRDPI 576
>TC221641 similar to UP|Q9LDX3 (Q9LDX3) T12C24.13 (F5O11.33), partial (40%)
Length = 750
Score = 32.0 bits (71), Expect = 0.68
Identities = 20/59 (33%), Positives = 31/59 (51%)
Frame = -1
Query: 360 SKGINSTFIALIPKVDNPQRLNDFRPISLVGSLYKILAKLLANRLRVVIGSVISDAQSA 418
+KG+ S+F K+ N L +F P+SL + +L +L N L I +VI A S+
Sbjct: 561 TKGLLSSFSPKFGKIPNSSSLVNFAPLSLAKKVKTLLVLVLVNLLSGNIFNVIPIAMSS 385
>TC225088 similar to UP|Q6WAT8 (Q6WAT8) Lipid tranfer protein II, complete
Length = 1006
Score = 30.4 bits (67), Expect = 2.0
Identities = 19/41 (46%), Positives = 23/41 (55%), Gaps = 1/41 (2%)
Frame = -1
Query: 237 HSVLASRRRRNSLCTIVVDGVVVEGVH-PIREAVFCHFENH 276
HSV ASRRRR+SL V D + H P R++ CH H
Sbjct: 487 HSVAASRRRRSSLLE-VTDAIGQATRHLPARDSCMCHGCTH 368
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.324 0.140 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,960,715
Number of Sequences: 63676
Number of extensions: 367618
Number of successful extensions: 2232
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 2218
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2231
length of query: 515
length of database: 12,639,632
effective HSP length: 101
effective length of query: 414
effective length of database: 6,208,356
effective search space: 2570259384
effective search space used: 2570259384
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146758.6