BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146756.7 - phase: 0
(96 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC216539 homologue to UP|PSA6_SOYBN (O48551) Proteasome subunit ... 165 3e-42
TC206026 UP|PSA6_SOYBN (O48551) Proteasome subunit alpha type 6 ... 165 3e-42
AW704170 weakly similar to SP|O48551|PSA6 Proteasome subunit alp... 112 3e-26
TC205194 homologue to UP|PSA7_CICAR (Q9SXU1) Proteasome subunit ... 67 1e-12
TC205193 homologue to UP|PSA7_CICAR (Q9SXU1) Proteasome subunit ... 67 2e-12
TC227911 UP|PSA5_SOYBN (Q9M4T8) Proteasome subunit alpha type 5 ... 66 2e-12
TC218552 UP|PSA5_SOYBN (Q9M4T8) Proteasome subunit alpha type 5 ... 66 2e-12
TC224607 homologue to UP|PSA4_SPIOL (P52427) Proteasome subunit ... 64 2e-11
TC224800 homologue to UP|PSA4_SPIOL (P52427) Proteasome subunit ... 64 2e-11
TC224606 homologue to UP|PSA4_SPIOL (P52427) Proteasome subunit ... 64 2e-11
TC215339 homologue to UP|PSA3_SPIOL (O24362) Proteasome subunit ... 62 6e-11
TC225504 homologue to UP|PSA2_ARATH (O23708) Proteasome subunit ... 62 6e-11
BE210574 61 8e-11
TC225503 homologue to UP|PSA2_ORYSA (Q9LSU2) Proteasome subunit ... 60 2e-10
TC225502 homologue to UP|PSA2_ARATH (O23708) Proteasome subunit ... 60 2e-10
TC204281 similar to UP|Q8H1Y2 (Q8H1Y2) 20S proteasome alpha 6 su... 50 2e-07
TC204277 similar to UP|Q8LF69 (Q8LF69) 20S proteasome subunit PA... 50 2e-07
AW277971 similar to GP|22947842|gb 20S proteasome alpha 6 subuni... 47 2e-06
BE802844 similar to SP|Q9SXU1|PSA7_ Proteasome subunit alpha typ... 45 8e-06
BU926842 29 0.33
>TC216539 homologue to UP|PSA6_SOYBN (O48551) Proteasome subunit alpha type 6
(20S proteasome alpha subunit A) (20S proteasome
subunit alpha-1) (Proteasome iota subunit) , complete
Length = 1073
Score = 165 bits (418), Expect = 3e-42
Identities = 81/84 (96%), Positives = 82/84 (97%)
Frame = +3
Query: 1 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKL 60
MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRG DS+CVVTQKKVPDKL
Sbjct: 162 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSICVVTQKKVPDKL 341
Query: 61 LDQTSVTHLFPITKYLGLLATGMT 84
LD TSVTHLFPITKYLGLLATGMT
Sbjct: 342 LDNTSVTHLFPITKYLGLLATGMT 413
>TC206026 UP|PSA6_SOYBN (O48551) Proteasome subunit alpha type 6 (20S
proteasome alpha subunit A) (20S proteasome subunit
alpha-1) (Proteasome iota subunit) , complete
Length = 1119
Score = 165 bits (418), Expect = 3e-42
Identities = 81/84 (96%), Positives = 82/84 (97%)
Frame = +2
Query: 1 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKL 60
MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRG DS+CVVTQKKVPDKL
Sbjct: 176 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSICVVTQKKVPDKL 355
Query: 61 LDQTSVTHLFPITKYLGLLATGMT 84
LD TSVTHLFPITKYLGLLATGMT
Sbjct: 356 LDNTSVTHLFPITKYLGLLATGMT 427
>AW704170 weakly similar to SP|O48551|PSA6 Proteasome subunit alpha type 6
(EC 3.4.25.1) (20S proteasome alpha subunit A), partial
(54%)
Length = 406
Score = 112 bits (280), Expect = 3e-26
Identities = 56/83 (67%), Positives = 65/83 (77%)
Frame = +2
Query: 2 SRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLL 61
SR SGGGYDRHIT+FSP+ LFQ EY FKAVKAA ITS ++ DS+CV TQ K P+KLL
Sbjct: 2 SRRSGGGYDRHITMFSPKRHLFQXEYTFKAVKAARITSSDIQKKDSICVGTQNKGPNKLL 181
Query: 62 DQTSVTHLFPITKYLGLLATGMT 84
+ TS+THLF ITKYL +AT MT
Sbjct: 182NNTSITHLFRITKYLKWIATDMT 250
>TC205194 homologue to UP|PSA7_CICAR (Q9SXU1) Proteasome subunit alpha type 7
(20S proteasome alpha subunit D) (20S proteasome
subunit alpha-4) , complete
Length = 1164
Score = 67.0 bits (162), Expect = 1e-12
Identities = 34/75 (45%), Positives = 48/75 (63%)
Frame = +3
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
YDR IT+FSP+G LFQVEYA +AV+ G ++GVRG D+V + +KK KL D SV
Sbjct: 162 YDRAITVFSPDGHLFQVEYALEAVR-KGNAAVGVRGTDNVVLGVEKKSTAKLQDSRSVRK 338
Query: 69 LFPITKYLGLLATGM 83
+ + ++ L G+
Sbjct: 339 IVNLDNHIALACAGL 383
>TC205193 homologue to UP|PSA7_CICAR (Q9SXU1) Proteasome subunit alpha type 7
(20S proteasome alpha subunit D) (20S proteasome
subunit alpha-4) , complete
Length = 1287
Score = 66.6 bits (161), Expect = 2e-12
Identities = 34/75 (45%), Positives = 48/75 (63%)
Frame = +2
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
YDR IT+FSP+G LFQVEYA +AV+ G ++GVRG D+V + +KK KL D SV
Sbjct: 167 YDRAITVFSPDGHLFQVEYALEAVR-KGNAAVGVRGTDNVVLGVEKKSTAKLQDSRSVRK 343
Query: 69 LFPITKYLGLLATGM 83
+ + ++ L G+
Sbjct: 344 IVNLDDHIALACAGL 388
>TC227911 UP|PSA5_SOYBN (Q9M4T8) Proteasome subunit alpha type 5 (20S
proteasome alpha subunit E) (20S proteasome subunit
alpha-5) , complete
Length = 1043
Score = 66.2 bits (160), Expect = 2e-12
Identities = 31/75 (41%), Positives = 50/75 (66%)
Frame = +1
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
YDR + FSPEGRLFQVEYA +A+K G T+IG++ + V + +K++ LL+ +SV
Sbjct: 112 YDRGVNTFSPEGRLFQVEYAIEAIK-LGSTAIGLKTKEGVVLAVEKRITSPLLEPSSVEK 288
Query: 69 LFPITKYLGLLATGM 83
+ I +++G +G+
Sbjct: 289 IMEIDEHIGCAMSGL 333
>TC218552 UP|PSA5_SOYBN (Q9M4T8) Proteasome subunit alpha type 5 (20S
proteasome alpha subunit E) (20S proteasome subunit
alpha-5) , complete
Length = 1031
Score = 66.2 bits (160), Expect = 2e-12
Identities = 31/75 (41%), Positives = 50/75 (66%)
Frame = +3
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
YDR + FSPEGRLFQVEYA +A+K G T+IG++ + V + +K++ LL+ +SV
Sbjct: 144 YDRGVNTFSPEGRLFQVEYAIEAIK-LGSTAIGLKTKEGVVLAVEKRITSPLLEPSSVEK 320
Query: 69 LFPITKYLGLLATGM 83
+ I +++G +G+
Sbjct: 321 IMEIDEHIGCAMSGL 365
>TC224607 homologue to UP|PSA4_SPIOL (P52427) Proteasome subunit alpha type 4
(20S proteasome alpha subunit C) (20S proteasome
subunit alpha-3) (Proteasome 27 kDa subunit) , partial
(56%)
Length = 489
Score = 63.5 bits (153), Expect = 2e-11
Identities = 35/76 (46%), Positives = 47/76 (61%), Gaps = 1/76 (1%)
Frame = +2
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLD-QTSVT 67
YD TIFSPEGRL+QVEYA +A+ AG T+IG+ D V +V +KKV KLL TS
Sbjct: 77 YDSRTTIFSPEGRLYQVEYAMEAIGNAG-TAIGILSKDGVVLVGEKKVTSKLLQTSTSTE 253
Query: 68 HLFPITKYLGLLATGM 83
++ I ++ G+
Sbjct: 254 KMYKIDDHVACAVAGI 301
>TC224800 homologue to UP|PSA4_SPIOL (P52427) Proteasome subunit alpha type 4
(20S proteasome alpha subunit C) (20S proteasome
subunit alpha-3) (Proteasome 27 kDa subunit) , partial
(98%)
Length = 1303
Score = 63.5 bits (153), Expect = 2e-11
Identities = 35/76 (46%), Positives = 47/76 (61%), Gaps = 1/76 (1%)
Frame = +2
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLD-QTSVT 67
YD TIFSPEGRL+QVEYA +A+ AG T+IG+ D V +V +KKV KLL TS
Sbjct: 164 YDSRTTIFSPEGRLYQVEYAMEAIGNAG-TAIGILSKDGVVLVGEKKVTSKLLQTSTSTE 340
Query: 68 HLFPITKYLGLLATGM 83
++ I ++ G+
Sbjct: 341 KMYKIDDHVACAVAGI 388
>TC224606 homologue to UP|PSA4_SPIOL (P52427) Proteasome subunit alpha type 4
(20S proteasome alpha subunit C) (20S proteasome
subunit alpha-3) (Proteasome 27 kDa subunit) , partial
(98%)
Length = 1002
Score = 63.5 bits (153), Expect = 2e-11
Identities = 35/76 (46%), Positives = 47/76 (61%), Gaps = 1/76 (1%)
Frame = +2
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLD-QTSVT 67
YD TIFSPEGRL+QVEYA +A+ AG T+IG+ D V +V +KKV KLL TS
Sbjct: 92 YDSRTTIFSPEGRLYQVEYAMEAIGNAG-TAIGILSKDGVVLVGEKKVTSKLLQTSTSTE 268
Query: 68 HLFPITKYLGLLATGM 83
++ I ++ G+
Sbjct: 269 KMYKIDDHVACAVAGI 316
>TC215339 homologue to UP|PSA3_SPIOL (O24362) Proteasome subunit alpha type 3
(20S proteasome alpha subunit G) (20S proteasome
subunit alpha-7) (Proteasome component C8) , complete
Length = 1091
Score = 61.6 bits (148), Expect = 6e-11
Identities = 30/78 (38%), Positives = 48/78 (61%)
Frame = +3
Query: 6 GGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTS 65
G GYD +T FSP+GR+FQ+EYA KAV +G T IG++ D V + +K +P K++ S
Sbjct: 87 GTGYDLSVTTFSPDGRVFQIEYAAKAVDNSG-TVIGIKCKDGVVLGVEKLIPSKMMLPGS 263
Query: 66 VTHLFPITKYLGLLATGM 83
+ + ++ G+ G+
Sbjct: 264 NRRIHSVHRHSGMAVAGL 317
>TC225504 homologue to UP|PSA2_ARATH (O23708) Proteasome subunit alpha type 2
(20S proteasome alpha subunit B) , complete
Length = 1151
Score = 61.6 bits (148), Expect = 6e-11
Identities = 30/75 (40%), Positives = 50/75 (66%)
Frame = +2
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
Y +T FSP G+L Q+E+A AV +G TS+G++ + V + T+KK+P L+D+TSV
Sbjct: 155 YSFSLTTFSPSGKLVQIEHALTAV-GSGQTSLGIKAANGVVIATEKKLPSILVDETSVQK 331
Query: 69 LFPITKYLGLLATGM 83
+ +T +G++ +GM
Sbjct: 332 IQLLTPNIGVVYSGM 376
>BE210574
Length = 400
Score = 61.2 bits (147), Expect = 8e-11
Identities = 34/75 (45%), Positives = 46/75 (61%), Gaps = 1/75 (1%)
Frame = +2
Query: 10 DRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLD-QTSVTH 68
D TIFSPEGRL+QVEYA +A+ AG T+IG+ D V +V +KKV KLL TS
Sbjct: 2 DSRTTIFSPEGRLYQVEYAMEAIGNAG-TAIGILSKDGVALVGEKKVTSKLLQTSTSTEK 178
Query: 69 LFPITKYLGLLATGM 83
++ I ++ G+
Sbjct: 179MYKIDDHVACAVAGI 223
>TC225503 homologue to UP|PSA2_ORYSA (Q9LSU2) Proteasome subunit alpha type 2
(20S proteasome alpha subunit B) (20S proteasome
subunit alpha-2) , partial (61%)
Length = 519
Score = 59.7 bits (143), Expect = 2e-10
Identities = 29/75 (38%), Positives = 49/75 (64%)
Frame = +1
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
Y +T FSP G+L Q+E+A AV +G TS+G++ + V + T+KK+P L+D+ SV
Sbjct: 106 YSFSLTTFSPSGKLVQIEHALTAV-GSGQTSLGIKAANGVVIATEKKLPSILVDEASVQK 282
Query: 69 LFPITKYLGLLATGM 83
+ +T +G++ +GM
Sbjct: 283 IQLLTPNIGVVYSGM 327
>TC225502 homologue to UP|PSA2_ARATH (O23708) Proteasome subunit alpha type 2
(20S proteasome alpha subunit B) , complete
Length = 1114
Score = 59.7 bits (143), Expect = 2e-10
Identities = 29/75 (38%), Positives = 49/75 (64%)
Frame = +1
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
Y +T FSP G+L Q+E+A AV +G TS+G++ + V + T+KK+P L+D+ SV
Sbjct: 103 YSFSLTTFSPSGKLVQIEHALTAV-GSGQTSLGIKAANGVVIATEKKLPSILVDEASVQK 279
Query: 69 LFPITKYLGLLATGM 83
+ +T +G++ +GM
Sbjct: 280 IQLLTPNIGVVYSGM 324
>TC204281 similar to UP|Q8H1Y2 (Q8H1Y2) 20S proteasome alpha 6 subunit,
partial (91%)
Length = 1275
Score = 50.1 bits (118), Expect = 2e-07
Identities = 28/76 (36%), Positives = 42/76 (54%)
Frame = +3
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
YD +T +SP GRLFQVEYA +AVK G +IG+R V + K +L +
Sbjct: 216 YDTDVTTWSPAGRLFQVEYAMEAVK-QGSAAIGLRSKTHVVLACVNKANSEL--SSHQKK 386
Query: 69 LFPITKYLGLLATGMT 84
+F + ++G+ G+T
Sbjct: 387 IFKVDNHIGVAIAGLT 434
>TC204277 similar to UP|Q8LF69 (Q8LF69) 20S proteasome subunit PAF1, partial
(92%)
Length = 1153
Score = 50.1 bits (118), Expect = 2e-07
Identities = 28/76 (36%), Positives = 42/76 (54%)
Frame = +3
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
YD +T +SP GRLFQVEYA +AVK G +IG+R V + K +L +
Sbjct: 90 YDTDVTTWSPAGRLFQVEYAMEAVK-QGSAAIGLRSKTHVVLACVNKANSEL--SSHQKK 260
Query: 69 LFPITKYLGLLATGMT 84
+F + ++G+ G+T
Sbjct: 261 IFKVDNHIGVAIAGLT 308
>AW277971 similar to GP|22947842|gb 20S proteasome alpha 6 subunit
{Nicotiana benthamiana}, partial (72%)
Length = 598
Score = 47.0 bits (110), Expect = 2e-06
Identities = 29/83 (34%), Positives = 45/83 (53%)
Frame = +2
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
YD +T ++P GRLFQVEYA +AVK G +IG+R V + K +L +
Sbjct: 14 YDTDVTTWNPAGRLFQVEYAMEAVK-QGSAAIGLRSKTHVVLGCVNKANSEL--SSHQKK 184
Query: 69 LFPITKYLGLLATGMTGVSFIAS 91
+F + ++G+ G+T S + S
Sbjct: 185IFKVDNHIGVGIAGLTVDSRVLS 253
>BE802844 similar to SP|Q9SXU1|PSA7_ Proteasome subunit alpha type 7 (EC
3.4.25.1) (20S proteasome alpha subunit D), partial
(38%)
Length = 292
Score = 44.7 bits (104), Expect = 8e-06
Identities = 28/70 (40%), Positives = 38/70 (54%)
Frame = +2
Query: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGNDSVCVVTQKKVPDKLLDQTSVTH 68
YDR +T+ S +G LFQVEYA AV GVRG D+V + +KK KL SV
Sbjct: 8 YDRAVTVCSHDGHLFQVEYAL*AVPRCN-ADRGVRGTDNVSLGVEKKCTVKLQYCWSVMK 184
Query: 69 LFPITKYLGL 78
+ + ++ L
Sbjct: 185IVNLDYHIAL 214
>BU926842
Length = 103
Score = 29.3 bits (64), Expect = 0.33
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +1
Query: 6 GGGYDRHITIFSPEGRLF 23
G GYD +T FSP+GR+F
Sbjct: 49 GTGYDLSVTTFSPDGRVF 102
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.139 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,325,055
Number of Sequences: 63676
Number of extensions: 32607
Number of successful extensions: 168
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of query: 96
length of database: 12,639,632
effective HSP length: 72
effective length of query: 24
effective length of database: 8,054,960
effective search space: 193319040
effective search space used: 193319040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC146756.7