BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146755.4 + phase: 0
(446 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC219158 similar to PIR|T46125|T46125 calmodulin-binding heat-sh... 206 5e-99
TC222290 similar to UP|Q41251 (Q41251) Calmodulin-binding heat-s... 287 6e-78
TC211055 similar to PIR|T46125|T46125 calmodulin-binding heat-sh... 223 1e-58
TC221708 similar to PIR|T46125|T46125 calmodulin-binding heat-sh... 167 2e-50
BM086218 similar to PIR|T04107|T041 calmodulin-binding heat-shoc... 137 7e-33
TC210644 82 5e-16
BU549111 homologue to GP|9757980|dbj| calmodulin-binding heat-sh... 70 3e-12
TC213490 weakly similar to GB|AAC13622.1|3047111|F6N23 F6N23.21 ... 65 5e-11
CD412485 59 3e-09
TC221873 similar to UP|Q41251 (Q41251) Calmodulin-binding heat-s... 50 3e-06
TC212956 weakly similar to UP|Q7XUE7 (Q7XUE7) OJ991113_30.24 pro... 34 0.12
TC219747 similar to UP|Q941F1 (Q941F1) At1g06800/F4H5_10, partia... 31 0.99
BU547263 30 2.2
BQ742585 30 2.2
TC214379 weakly similar to UP|Q6Z307 (Q6Z307) Lipase class 3-lik... 30 2.9
TC224630 similar to UP|Q8LKG0 (Q8LKG0) Drm4, partial (98%) 29 3.7
BM086022 29 3.7
AI900009 similar to GP|21552998|gb Drm3 {Pisum sativum}, partial... 29 3.7
BG154625 similar to GP|21553000|gb Drm4 {Pisum sativum}, partial... 29 3.7
TC222475 29 4.9
>TC219158 similar to PIR|T46125|T46125 calmodulin-binding heat-shock-like
protein - Arabidopsis thaliana {Arabidopsis thaliana;} ,
partial (39%)
Length = 1074
Score = 206 bits (524), Expect(2) = 5e-99
Identities = 99/139 (71%), Positives = 112/139 (80%)
Frame = +3
Query: 161 RELVEKYPNYTLTFAGHSLGSGVAAALSMVVVQNRDRLGNIERKRVRCYAIAPARCMSLN 220
REL + P Y L F GHSLG+GV A L+M+ V NRD+LG I R ++RC+AIA RC SLN
Sbjct: 60 RELAAENPTYMLIFTGHSLGAGVVALLTMLAVHNRDKLG-ISRNKIRCFAIASPRCASLN 236
Query: 221 LAVRYADVINSVVLQDDFLPRTATPLEDIFKSLFCLPCLLCLRCMRDTCIPEEKMLKDPR 280
LAVRYADVINSVVLQDDFLPRT LED+FKSL C PCLLC+ C++DTC EEK L+DPR
Sbjct: 237 LAVRYADVINSVVLQDDFLPRTTAALEDVFKSLLCWPCLLCIMCLKDTCTLEEKKLRDPR 416
Query: 281 RLYAPGRLYHIVERKPFRL 299
RLYAPGRLYHIVERKPF L
Sbjct: 417 RLYAPGRLYHIVERKPFSL 473
Score = 173 bits (439), Expect(2) = 5e-99
Identities = 90/152 (59%), Positives = 114/152 (74%), Gaps = 3/152 (1%)
Frame = +1
Query: 298 RLGRFPPVVRTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAQRALDLMMEKDNTMEVPAK 357
R+GR PPVVRTAVPVDGRFEH+VLSCNATSDHAIIWIE+E++RALD+M + D ME+PA+
Sbjct: 484 RIGRLPPVVRTAVPVDGRFEHLVLSCNATSDHAIIWIERESRRALDIMRDNDEHMEIPAE 663
Query: 358 QIMQRQKTMTR-HGQEYKAALQRAKTLDIPHAFTPPSEYGTFD--EEGEESSRSEAESSV 414
Q M+R +++ R H +EY AALQRA LDIP A++ PS YGTF + GE+S RS A S
Sbjct: 664 QRMERHESIAREHSEEYHAALQRAVALDIPQAYS-PSSYGTFHQIDVGEDSGRSSALGSS 840
Query: 415 SSTNRSTVNESWDVLIERLFDKDEHGHMVLKR 446
+ T +SW+ + RLFD D+ GHMV K+
Sbjct: 841 LFHKKRT--QSWNEFVGRLFDVDDAGHMVFKK 930
>TC222290 similar to UP|Q41251 (Q41251) Calmodulin-binding heat-shock
protein, partial (44%)
Length = 843
Score = 287 bits (735), Expect = 6e-78
Identities = 146/249 (58%), Positives = 190/249 (75%), Gaps = 4/249 (1%)
Frame = +2
Query: 196 DRLGNIERKRVRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLPRTATPLEDIFKSLFC 255
DRLG I ++++RCYA+APARCMSLNLAV+YA+VI+S+VLQDDFLPRTATPLEDIFKS+FC
Sbjct: 2 DRLGGIPKEKIRCYALAPARCMSLNLAVKYANVIHSIVLQDDFLPRTATPLEDIFKSIFC 181
Query: 256 LPCLLCLRCMRDTCIPEEKMLKDPRRLYAPGRLYHIVERKPFRLGRFPPVVRTAVPVDGR 315
LPCLL L C+RDT IPE + L+DPRRLYAPGR+YHI+ERK R GRFPP VRTA+PVDGR
Sbjct: 182 LPCLLFLVCLRDTFIPEGRKLRDPRRLYAPGRMYHILERKFCRCGRFPPEVRTAIPVDGR 361
Query: 316 FEHIVLSCNATSDHAIIWIEKEAQRALDLM--MEKDNTMEVPAKQIMQRQKTMTRHGQEY 373
FEHIVLSCNATSDH IIWIE+EA++AL LM + + P Q QR KT+ + E+
Sbjct: 362 FEHIVLSCNATSDHGIIWIEREAEKALQLMKAQSSETVTDPPTVQKFQRLKTIEK---EH 532
Query: 374 KAALQRAKTLDIPHAFTPPSEYGTFDEEGEESSRSEAESSVSSTNRSTVNES-WDVLIER 432
+ AL+RA +L++PHA + + EG+++S + + S+ ++S+ S WD ++E+
Sbjct: 533 RDALERAVSLNVPHAVDTAENEPSENNEGDDASGNGRNNVSSNQSKSSGGRSNWDDVVEK 712
Query: 433 LF-DKDEHG 440
L +K E G
Sbjct: 713 LLKNKRETG 739
>TC211055 similar to PIR|T46125|T46125 calmodulin-binding heat-shock-like
protein - Arabidopsis thaliana {Arabidopsis thaliana;} ,
partial (19%)
Length = 597
Score = 223 bits (569), Expect = 1e-58
Identities = 110/139 (79%), Positives = 124/139 (89%), Gaps = 1/139 (0%)
Frame = +2
Query: 309 AVPVDGRFEHIVLSCNATSDHAIIWIEKEAQRALDLMMEKDNTMEVPAKQIMQRQKTMTR 368
AVPVDGRFEHIVLSCNATSDHAIIWIEKEAQRALDLM+EKD ME PAKQ M+RQ+T+TR
Sbjct: 2 AVPVDGRFEHIVLSCNATSDHAIIWIEKEAQRALDLMLEKDQPMEPPAKQKMERQETLTR 181
Query: 369 HGQEYKAALQRAKTLDIPHAFTPPSEYGTFDEEGEE-SSRSEAESSVSSTNRSTVNESWD 427
H EYKAALQRAKTLD+PHA+TPPSEYGTFD+EG+E S+RS+ ESS N+S+V+ESWD
Sbjct: 182 HNDEYKAALQRAKTLDVPHAYTPPSEYGTFDDEGDENSTRSQGESS----NKSSVDESWD 349
Query: 428 VLIERLFDKDEHGHMVLKR 446
LIER FDKDEHGH VLK+
Sbjct: 350 ALIERHFDKDEHGHTVLKK 406
>TC221708 similar to PIR|T46125|T46125 calmodulin-binding heat-shock-like
protein - Arabidopsis thaliana {Arabidopsis thaliana;} ,
partial (16%)
Length = 556
Score = 167 bits (422), Expect(2) = 2e-50
Identities = 90/154 (58%), Positives = 117/154 (75%), Gaps = 5/154 (3%)
Frame = +2
Query: 298 RLGRFPPVVRTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAQRALDLMMEKDNT-MEVPA 356
R+GR PPVVRTAVPVDGRFEH+VLSCNATSDHAIIWIE+E++RALDLM +KD ME+P
Sbjct: 95 RIGRLPPVVRTAVPVDGRFEHLVLSCNATSDHAIIWIERESRRALDLMRDKDEQHMEIPE 274
Query: 357 KQIMQRQKTMTR-HGQEYKAALQRAKTLDIPHAFTPPSEYGTFD--EEGEESSRSEA-ES 412
+Q M+R +++ R H +EY AAL+RA LDIP A++ PS YGTF+ + GE+S RS S
Sbjct: 275 EQKMERHESIAREHSEEYHAALKRAVALDIPQAYS-PSLYGTFNQIDVGEDSGRSSVLGS 451
Query: 413 SVSSTNRSTVNESWDVLIERLFDKDEHGHMVLKR 446
S+S R+ ++W+ + RLFD D+ GHMV K+
Sbjct: 452 SLSHKKRA---QNWNEFVGRLFDVDDSGHMVFKK 544
Score = 50.4 bits (119), Expect(2) = 2e-50
Identities = 22/26 (84%), Positives = 23/26 (87%)
Frame = +1
Query: 272 EEKMLKDPRRLYAPGRLYHIVERKPF 297
EEK L+DP LYAPGRLYHIVERKPF
Sbjct: 1 EEKKLRDPXXLYAPGRLYHIVERKPF 78
>BM086218 similar to PIR|T04107|T041 calmodulin-binding heat-shock protein -
common tobacco, partial (8%)
Length = 430
Score = 137 bits (346), Expect = 7e-33
Identities = 63/114 (55%), Positives = 78/114 (68%)
Frame = +1
Query: 1 MSIICGLPLVECVYCLACVRWAWKRCLHTAGHDSETWGFAATQEFEPVPRLCRYILAVYE 60
MS CG+ VECV+ L C RW WKRC + +DS TW A EF+PVPR+CR ILA YE
Sbjct: 112 MSASCGV--VECVFVLGCARWLWKRCTYVGSYDSATWPSATADEFDPVPRVCRLILANYE 285
Query: 61 DDLRNPLWAPPGGYGINPDWLLLRKTYKDTRGRAPPYILYLDHDHADIVLAIRG 114
DLR P + +NPD ++ R TY+DT G APPY++YLDHDH +IVLA+RG
Sbjct: 286 PDLRTP------NHRLNPDCIIKRVTYEDTLGHAPPYVIYLDHDHKEIVLAVRG 429
>TC210644
Length = 522
Score = 82.0 bits (201), Expect = 5e-16
Identities = 50/108 (46%), Positives = 72/108 (66%), Gaps = 5/108 (4%)
Frame = +3
Query: 344 LMMEKDNT-MEVPAKQIMQRQKTMTR-HGQEYKAALQRAKTLDIPHAFTPPSEYGTFDE- 400
LM +KD ME+P +Q M+R +++ R H +EY AAL+RA LDIP A++P S YGTF++
Sbjct: 198 LMRDKDEQHMEIPEEQKMERHESIAREHSEEYHAALKRAVALDIPQAYSP-SLYGTFNQI 374
Query: 401 -EGEESSRSEA-ESSVSSTNRSTVNESWDVLIERLFDKDEHGHMVLKR 446
GE+S RS SS+S R+ ++W+ + RLFD D+ GHMV K+
Sbjct: 375 DVGEDSGRSSVLGSSLSHKKRA---QNWNEFVGRLFDVDDSGHMVFKK 509
>BU549111 homologue to GP|9757980|dbj| calmodulin-binding heat-shock protein
{Arabidopsis thaliana}, partial (9%)
Length = 665
Score = 69.7 bits (169), Expect = 3e-12
Identities = 31/40 (77%), Positives = 35/40 (87%)
Frame = +2
Query: 256 LPCLLCLRCMRDTCIPEEKMLKDPRRLYAPGRLYHIVERK 295
LPCLL L C+RDT IPE + L+DPRRLYAPGR+YHIVERK
Sbjct: 158 LPCLLFLVCLRDTFIPEGRKLRDPRRLYAPGRMYHIVERK 277
>TC213490 weakly similar to GB|AAC13622.1|3047111|F6N23 F6N23.21 {Arabidopsis
thaliana;} , partial (9%)
Length = 715
Score = 65.5 bits (158), Expect = 5e-11
Identities = 25/38 (65%), Positives = 31/38 (80%)
Frame = +1
Query: 1 MSIICGLPLVECVYCLACVRWAWKRCLHTAGHDSETWG 38
MS ICGLP++ECVYCLAC R WK+CL+T ++SE WG
Sbjct: 601 MSAICGLPILECVYCLACAR*VWKKCLYTGCYESENWG 714
>CD412485
Length = 319
Score = 59.3 bits (142), Expect = 3e-09
Identities = 29/44 (65%), Positives = 35/44 (78%)
Frame = -3
Query: 403 EESSRSEAESSVSSTNRSTVNESWDVLIERLFDKDEHGHMVLKR 446
E S+RS+ ESS N+S+V+ESWD LIER FDKDEHGH VLK+
Sbjct: 311 ENSTRSQGESS----NKSSVDESWDALIERHFDKDEHGHTVLKK 192
>TC221873 similar to UP|Q41251 (Q41251) Calmodulin-binding heat-shock
protein, partial (10%)
Length = 517
Score = 49.7 bits (117), Expect = 3e-06
Identities = 31/101 (30%), Positives = 56/101 (54%), Gaps = 3/101 (2%)
Frame = +3
Query: 349 DNTMEVPAKQIMQRQKTMTRHGQEYKAALQRAKTLDIPHAFTPPSEYGTFDEEGEESSRS 408
+ + P Q QR KT+ + E++ AL+RA +L++PHA + + + EG++ + +
Sbjct: 15 ETVTDPPTVQKFQRLKTIEK---EHRDALERAVSLNVPHAVDTAEKETSENNEGDDDASA 185
Query: 409 EAESSVSS--TNRSTVNESWDVLIERLF-DKDEHGHMVLKR 446
A ++VSS + S +WD ++E+L K E G L+R
Sbjct: 186 SANNNVSSNQSKSSGGRSNWDEVVEKLLKKKSETGEQNLER 308
>TC212956 weakly similar to UP|Q7XUE7 (Q7XUE7) OJ991113_30.24 protein,
partial (17%)
Length = 448
Score = 34.3 bits (77), Expect = 0.12
Identities = 20/72 (27%), Positives = 35/72 (47%)
Frame = +3
Query: 170 YTLTFAGHSLGSGVAAALSMVVVQNRDRLGNIERKRVRCYAIAPARCMSLNLAVRYADVI 229
Y + GHSLG +AA L + Q +R N+ Y+ P C+ L +A ++ +
Sbjct: 153 YNVRIVGHSLGGAIAALLGL---QLYNRYPNL-----HVYSYGPLPCLDLVVANACSEFV 308
Query: 230 NSVVLQDDFLPR 241
S++ ++F R
Sbjct: 309 TSIIFGNEFSSR 344
>TC219747 similar to UP|Q941F1 (Q941F1) At1g06800/F4H5_10, partial (42%)
Length = 1061
Score = 31.2 bits (69), Expect = 0.99
Identities = 17/47 (36%), Positives = 28/47 (59%), Gaps = 2/47 (4%)
Frame = +2
Query: 160 LRELVEKYPN--YTLTFAGHSLGSGVAAALSMVVVQNRDRLGNIERK 204
++ L+EKY + ++T AGHSLGS +A + +V+ +G RK
Sbjct: 821 VKRLMEKYADEEVSVTIAGHSLGSAMAILSAFDIVETGVNVGKDGRK 961
>BU547263
Length = 566
Score = 30.0 bits (66), Expect = 2.2
Identities = 16/54 (29%), Positives = 27/54 (49%)
Frame = +3
Query: 233 VLQDDFLPRTATPLEDIFKSLFCLPCLLCLRCMRDTCIPEEKMLKDPRRLYAPG 286
++QD +P+ +TP I S C C++ R + C P ++ +K L PG
Sbjct: 246 IIQDG*IPKISTPKTSIH*SRGC--CIVTTRGLVTVCFPSDQTMKPLFTLEPPG 401
>BQ742585
Length = 426
Score = 30.0 bits (66), Expect = 2.2
Identities = 12/28 (42%), Positives = 17/28 (59%)
Frame = +1
Query: 400 EEGEESSRSEAESSVSSTNRSTVNESWD 427
+ GE RS+ +S+ STN+ T N WD
Sbjct: 256 DSGESQQRSDDQSTPVSTNKKTENMEWD 339
>TC214379 weakly similar to UP|Q6Z307 (Q6Z307) Lipase class 3-like, partial
(54%)
Length = 1306
Score = 29.6 bits (65), Expect = 2.9
Identities = 31/121 (25%), Positives = 51/121 (41%), Gaps = 20/121 (16%)
Frame = +1
Query: 98 ILYLD-HDHADI-VLAIRGLNLARESDYAVLLDNKL----GKRKFDGGYVHN-GLLKAAG 150
++ LD H++ D V+A RG + LD G K GG++ GL K G
Sbjct: 187 LIVLDKHENRDTYVVAFRGTEPFDADAWCTDLDISWYGIPGIGKMHGGFMKALGLQKNVG 366
Query: 151 WVMDAECE-------------ILRELVEKYPNYTLTFAGHSLGSGVAAALSMVVVQNRDR 197
W + + + ILR+ + + PN GHSLG +A ++ + ++
Sbjct: 367 WPKEIQRDENLPPLAYYVIRDILRKGLSENPNAKFIITGHSLGGALAILYPTIMFLHDEK 546
Query: 198 L 198
L
Sbjct: 547 L 549
>TC224630 similar to UP|Q8LKG0 (Q8LKG0) Drm4, partial (98%)
Length = 806
Score = 29.3 bits (64), Expect = 3.7
Identities = 13/31 (41%), Positives = 19/31 (60%)
Frame = -3
Query: 67 LWAPPGGYGINPDWLLLRKTYKDTRGRAPPY 97
LW PPGG+ I ++LR T + G++ PY
Sbjct: 255 LWLPPGGFTI----MILRVTLTASSGKSSPY 175
>BM086022
Length = 427
Score = 29.3 bits (64), Expect = 3.7
Identities = 14/39 (35%), Positives = 21/39 (52%)
Frame = +3
Query: 280 RRLYAPGRLYHIVERKPFRLGRFPPVVRTAVPVDGRFEH 318
R L+APG ++H+ PF L R P++ + RF H
Sbjct: 270 RTLFAPGGVFHVRRSSPFHLRR-SPLLHLQDRMQPRFRH 383
>AI900009 similar to GP|21552998|gb Drm3 {Pisum sativum}, partial (82%)
Length = 422
Score = 29.3 bits (64), Expect = 3.7
Identities = 13/31 (41%), Positives = 19/31 (60%)
Frame = -3
Query: 67 LWAPPGGYGINPDWLLLRKTYKDTRGRAPPY 97
LW PPGG+ I ++LR T + G++ PY
Sbjct: 294 LWLPPGGFTI----MILRVTLTASSGKSSPY 214
>BG154625 similar to GP|21553000|gb Drm4 {Pisum sativum}, partial (82%)
Length = 430
Score = 29.3 bits (64), Expect = 3.7
Identities = 13/31 (41%), Positives = 19/31 (60%)
Frame = -2
Query: 67 LWAPPGGYGINPDWLLLRKTYKDTRGRAPPY 97
LW PPGG+ I ++LR T + G++ PY
Sbjct: 249 LWLPPGGFTI----MILRVTLTASSGKSSPY 169
>TC222475
Length = 557
Score = 28.9 bits (63), Expect = 4.9
Identities = 12/28 (42%), Positives = 16/28 (56%), Gaps = 3/28 (10%)
Frame = +1
Query: 7 LPLVECVYCLACVRWAWKR---CLHTAG 31
LP+ EC + AC RW W C+H +G
Sbjct: 403 LPISEC-FVYACTRWVWLHVCICIHCSG 483
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.323 0.139 0.435
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,967,941
Number of Sequences: 63676
Number of extensions: 334325
Number of successful extensions: 1707
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 1690
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1697
length of query: 446
length of database: 12,639,632
effective HSP length: 100
effective length of query: 346
effective length of database: 6,272,032
effective search space: 2170123072
effective search space used: 2170123072
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146755.4