BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146751.9 - phase: 0
(186 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC207882 heat shock protein DnaJ [Glycine max] 227 3e-60
BM892801 similar to GP|4455312|emb| DnaJ-like protein {Arabidops... 170 3e-43
TC207610 homologue to UP|P93499 (P93499) DnaJ-like protein (Frag... 71 3e-13
TC230569 similar to UP|O65641 (O65641) DnaJ-like protein, partia... 70 6e-13
TC217143 similar to UP|P93499 (P93499) DnaJ-like protein (Fragme... 69 2e-12
TC217144 homologue to UP|P93499 (P93499) DnaJ-like protein (Frag... 69 2e-12
TC209496 similar to UP|P93499 (P93499) DnaJ-like protein (Fragme... 68 2e-12
TC230421 weakly similar to UP|O65641 (O65641) DnaJ-like protein,... 67 4e-12
TC205833 similar to GB|AAM78044.1|21928031|AY125534 At3g62600/F2... 66 1e-11
TC210562 weakly similar to GB|AAP88343.1|32815917|BT009709 At3g1... 65 2e-11
TC218967 similar to UP|Q8DH11 (Q8DH11) DnaJ protein, partial (10%) 65 2e-11
TC218966 weakly similar to UP|Q74ZX4 (Q74ZX4) AGR084Cp, partial ... 65 2e-11
TC231382 weakly similar to GB|AAP21357.1|30102878|BT006549 At1g5... 63 7e-11
BF596888 63 7e-11
TC220213 similar to UP|Q7XYV0 (Q7XYV0) P58IPK, partial (38%) 63 7e-11
TC205941 homologue to UP|Q8H2B1 (Q8H2B1) DnaJ-like protein, comp... 63 9e-11
TC222989 weakly similar to UP|DJB6_HUMAN (O75190) DnaJ homolog s... 62 1e-10
TC228163 similar to UP|Q8LEU4 (Q8LEU4) DnaJ protein-like, partia... 62 1e-10
TC211892 similar to UP|Q9FEW7 (Q9FEW7) DnaJ like protein, partia... 62 1e-10
TC231504 similar to UP|Q9FEW7 (Q9FEW7) DnaJ like protein, partia... 62 2e-10
>TC207882 heat shock protein DnaJ [Glycine max]
Length = 727
Score = 227 bits (578), Expect = 3e-60
Identities = 125/191 (65%), Positives = 144/191 (74%), Gaps = 5/191 (2%)
Frame = +3
Query: 1 MRCYGLTIPGSDHRLITPTLPPSLSFRPTTIPMTRISFGASFPKLKATF-NDGMAAEERS 59
MR YGLT+PGSDHR PT+ + FRP P+ RI+F KAT NDG + S
Sbjct: 48 MRSYGLTVPGSDHRFCVPTVS-QVFFRPNR-PINRIAFP------KATLKNDGFVVD-LS 200
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRR 119
FYDLLGIPESGS+ +IK AYKQLARKYHPDVSPP RV+EYTKRFIQVQEAYETLSDPSRR
Sbjct: 201 FYDLLGIPESGSVTEIKNAYKQLARKYHPDVSPPGRVEEYTKRFIQVQEAYETLSDPSRR 380
Query: 120 IMYDRDMAIGIHLAFNACKCYNHSDQVSEQKGD----WKSQLAGLQRRSDSKVAAENMSW 175
MYD+DMA GI+ AFNA + YN+ DQV EQK + WKSQL+ L+R+S+ K A NMSW
Sbjct: 381 AMYDKDMAKGINFAFNARRRYNYHDQVVEQKSEWKARWKSQLSELKRKSNGKDAGGNMSW 560
Query: 176 AARVRQQRGEL 186
AAR+RQQR EL
Sbjct: 561 AARMRQQRDEL 593
>BM892801 similar to GP|4455312|emb| DnaJ-like protein {Arabidopsis
thaliana}, partial (34%)
Length = 422
Score = 170 bits (431), Expect = 3e-43
Identities = 94/148 (63%), Positives = 106/148 (71%)
Frame = +3
Query: 1 MRCYGLTIPGSDHRLITPTLPPSLSFRPTTIPMTRISFGASFPKLKATFNDGMAAEERSF 60
M+CYGLT+PGS PT+ P I TRI+F PK N G E SF
Sbjct: 9 MQCYGLTVPGS-----VPTVSPVFLRPNRPIIATRIAF----PKATLNKNQGFVVE-LSF 158
Query: 61 YDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRRI 120
Y+LLGIPES S+ +IK AYKQLARKYHPDVSPP RV+EYTKRFIQVQEAYETLSDPSRR
Sbjct: 159 YELLGIPESVSVTEIKNAYKQLARKYHPDVSPPGRVEEYTKRFIQVQEAYETLSDPSRRA 338
Query: 121 MYDRDMAIGIHLAFNACKCYNHSDQVSE 148
MYD+DMA GI+LAFNA + YN+ DQV E
Sbjct: 339 MYDKDMARGINLAFNARRRYNYHDQVVE 422
>TC207610 homologue to UP|P93499 (P93499) DnaJ-like protein (Fragment),
partial (93%)
Length = 754
Score = 70.9 bits (172), Expect = 3e-13
Identities = 33/68 (48%), Positives = 43/68 (62%)
Frame = +3
Query: 59 SFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSR 118
S YD+LGIP S +IK AY++LAR HPDV+ DR F+++ AY TLSDP +
Sbjct: 243 SLYDILGIPAGASNQEIKAAYRRLARVCHPDVAAIDRKNSSADEFMKIHAAYSTLSDPDK 422
Query: 119 RIMYDRDM 126
R YDR +
Sbjct: 423 RANYDRSL 446
>TC230569 similar to UP|O65641 (O65641) DnaJ-like protein, partial (27%)
Length = 549
Score = 70.1 bits (170), Expect = 6e-13
Identities = 31/68 (45%), Positives = 45/68 (65%)
Frame = +1
Query: 59 SFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSR 118
S YD+LGI S ++IK AY++LAR YHPDV ++ + +F+ + AY TLSDP +
Sbjct: 169 SLYDVLGISVGASCIEIKAAYRKLARTYHPDVVAMNQKESSANQFMMIHSAYSTLSDPEK 348
Query: 119 RIMYDRDM 126
R YDR++
Sbjct: 349 RAQYDREI 372
>TC217143 similar to UP|P93499 (P93499) DnaJ-like protein (Fragment), partial
(91%)
Length = 521
Score = 68.6 bits (166), Expect = 2e-12
Identities = 31/66 (46%), Positives = 43/66 (64%)
Frame = +3
Query: 59 SFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSR 118
S Y++LGIP S +IK AY++LAR +HPDV+ DR F+++ AY TLSDP +
Sbjct: 234 SLYEVLGIPAGASNQEIKAAYRRLARVFHPDVAAIDRKNSSADEFMKIHAAYSTLSDPDK 413
Query: 119 RIMYDR 124
R YD+
Sbjct: 414 RANYDQ 431
>TC217144 homologue to UP|P93499 (P93499) DnaJ-like protein (Fragment),
partial (71%)
Length = 541
Score = 68.6 bits (166), Expect = 2e-12
Identities = 31/66 (46%), Positives = 43/66 (64%)
Frame = +2
Query: 59 SFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSR 118
S Y++LGIP S +IK AY++LAR +HPDV+ DR F+++ AY TLSDP +
Sbjct: 59 SLYEVLGIPAGASNQEIKAAYRRLARVFHPDVAAIDRKNSSADEFMKIHAAYSTLSDPDK 238
Query: 119 RIMYDR 124
R YD+
Sbjct: 239 RANYDQ 256
>TC209496 similar to UP|P93499 (P93499) DnaJ-like protein (Fragment), partial
(65%)
Length = 718
Score = 68.2 bits (165), Expect = 2e-12
Identities = 43/123 (34%), Positives = 62/123 (49%), Gaps = 14/123 (11%)
Frame = +2
Query: 18 PTLPPSLSFRPTTIPMTRISFGASFPKLKATFND--------------GMAAEERSFYDL 63
PT +LS T P + F + ATF + GMA + YD+
Sbjct: 134 PTSTVTLSGESVTSPPRHVRFRSVVSFATATFTEEQQHKAAPYLRPHHGMALSA-TLYDI 310
Query: 64 LGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRRIMYD 123
LGI + S +I+ AY++LAR HPDV+P +R + F+++ AY TLSDP +R YD
Sbjct: 311 LGIRATASGEEIRAAYRRLARVCHPDVAPVERKESSAGEFMKIHAAYCTLSDPEKRDSYD 490
Query: 124 RDM 126
R +
Sbjct: 491 RSL 499
>TC230421 weakly similar to UP|O65641 (O65641) DnaJ-like protein, partial
(37%)
Length = 772
Score = 67.4 bits (163), Expect = 4e-12
Identities = 50/150 (33%), Positives = 74/150 (49%), Gaps = 17/150 (11%)
Frame = +3
Query: 11 SDHRLITPTLPPSLSFRPTTIPM---TRISFGASFPK---LKATFNDGMAAEER--SFYD 62
S RL+ + P+L+ IP+ + + ASFP+ ++A + + S YD
Sbjct: 63 SSQRLVQFPMAPTLNLSAVGIPLRFSSSVDRSASFPRRNSVRAVAEEAVETRRSAASLYD 242
Query: 63 LLGIPESGSLMDIKRAYKQLARKYHPDVS-----PPDRVKEYTKR-FIQVQEAYETLSDP 116
+L + S +IK AY+ LA+ HPD + D Y FIQ++ AYETLSDP
Sbjct: 243 VLRVERDASPTEIKSAYRSLAKLLHPDAAVQRSPETDGGGGYVDGDFIQLRNAYETLSDP 422
Query: 117 SRRIMYDRDMAI---GIHLAFNACKCYNHS 143
S + +YDR +A G H F+ NHS
Sbjct: 423 SAKAIYDRTLAAPHGGRHRRFSTPLSRNHS 512
>TC205833 similar to GB|AAM78044.1|21928031|AY125534 At3g62600/F26K9_30
{Arabidopsis thaliana;} , partial (55%)
Length = 866
Score = 65.9 bits (159), Expect = 1e-11
Identities = 32/72 (44%), Positives = 48/72 (66%)
Frame = +1
Query: 53 MAAEERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYET 112
+A +S+YD+L + + S IKRAY++LA KYHPD +P + +E K+F ++ AYE
Sbjct: 262 IAIAGKSYYDILQLSKGASDEQIKRAYRKLALKYHPDKNPGN--EEANKKFAEISNAYEV 435
Query: 113 LSDPSRRIMYDR 124
LSD +R +YDR
Sbjct: 436 LSDSEKRNIYDR 471
>TC210562 weakly similar to GB|AAP88343.1|32815917|BT009709 At3g13310
{Arabidopsis thaliana;} , partial (22%)
Length = 527
Score = 64.7 bits (156), Expect = 2e-11
Identities = 44/107 (41%), Positives = 58/107 (54%)
Frame = +2
Query: 17 TPTLPPSLSFRPTTIPMTRISFGASFPKLKATFNDGMAAEERSFYDLLGIPESGSLMDIK 76
T L P T P RIS A AT D A S Y++L I ++ S ++IK
Sbjct: 77 TLNLTPLTGAGRTLRPPFRISIRA----FTATAIDSRRAA--SLYEVLRIKQNASAVEIK 238
Query: 77 RAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRRIMYD 123
AY+ LA+ YHPD S R + + FI++ +AYETLSDPS R +YD
Sbjct: 239 SAYRNLAKVYHPD-SALRRSESDERDFIEIHDAYETLSDPSARALYD 376
>TC218967 similar to UP|Q8DH11 (Q8DH11) DnaJ protein, partial (10%)
Length = 744
Score = 64.7 bits (156), Expect = 2e-11
Identities = 42/131 (32%), Positives = 73/131 (55%), Gaps = 7/131 (5%)
Frame = +1
Query: 57 ERSFYDLLGI-PESGSLMDIKRAYKQLARKYHPDV-SPPDRVKEYTKRFIQVQEAYETLS 114
+ + Y +L + P S ++ +IKRAY+ +A +YHPDV P +E T+ F+Q+ AYETLS
Sbjct: 229 QENLYKILSVSPGSATMDEIKRAYRSMALQYHPDVCHDPSMKEESTRMFVQLNAAYETLS 408
Query: 115 DPSRRIMYDRDMAIGIH-LAFNACKCYNHSDQVSEQKGDWKS----QLAGLQRRSDSKVA 169
+P R YD ++ + ++ ++ S VS + W+S Q+ L+ RS ++
Sbjct: 409 NPRLREQYDSELGLRSEVMSVSSDHERWRSVSVSSEHERWESRFQEQVIELKTRSRRRMG 588
Query: 170 AENMSWAARVR 180
+ S +R+R
Sbjct: 589 QKGGSSGSRMR 621
>TC218966 weakly similar to UP|Q74ZX4 (Q74ZX4) AGR084Cp, partial (7%)
Length = 769
Score = 64.7 bits (156), Expect = 2e-11
Identities = 41/132 (31%), Positives = 69/132 (52%), Gaps = 2/132 (1%)
Frame = +3
Query: 57 ERSFYDLLGI-PESGSLMDIKRAYKQLARKYHPDV-SPPDRVKEYTKRFIQVQEAYETLS 114
+ + Y +L + P S ++ +IKRAY+ +A +YHPDV P +E T+ F+Q+ AY+TLS
Sbjct: 288 QENLYKILSVCPGSATMDEIKRAYRSMALQYHPDVCHDPSMKEESTRMFVQLNTAYKTLS 467
Query: 115 DPSRRIMYDRDMAIGIHLAFNACKCYNHSDQVSEQKGDWKSQLAGLQRRSDSKVAAENMS 174
+P R YD ++ +G S ++S D +R V++E+
Sbjct: 468 NPRLREEYDSELGLG-------------STEMSTVSSDHH------ERWGSVSVSSEHER 590
Query: 175 WAARVRQQRGEL 186
W +R ++Q EL
Sbjct: 591 WESRFQEQMIEL 626
>TC231382 weakly similar to GB|AAP21357.1|30102878|BT006549 At1g56300
{Arabidopsis thaliana;} , partial (59%)
Length = 973
Score = 63.2 bits (152), Expect = 7e-11
Identities = 31/82 (37%), Positives = 49/82 (58%), Gaps = 2/82 (2%)
Frame = +3
Query: 44 KLKATFNDGMAAEERSFYDLLGIPESGSLMDIKRAYKQLARKYHPD--VSPPDRVKEYTK 101
KL T + S+Y++LG+ ++ +I+RAY++LA ++HPD P + E +
Sbjct: 96 KLIVTMDARGEGSSTSYYNVLGVSSDSNVDEIRRAYRKLAMQWHPDKCTRSPSLLGEAKR 275
Query: 102 RFIQVQEAYETLSDPSRRIMYD 123
+F Q+QEAY LSD +R MYD
Sbjct: 276 KFQQIQEAYSVLSDSKKRTMYD 341
>BF596888
Length = 418
Score = 63.2 bits (152), Expect = 7e-11
Identities = 40/119 (33%), Positives = 62/119 (51%), Gaps = 1/119 (0%)
Frame = +2
Query: 21 PPSLSFRPTTIPM-TRISFGASFPKLKATFNDGMAAEERSFYDLLGIPESGSLMDIKRAY 79
P + +R T IP + F ++ +A + D E YDLLG+ +S + +IK+AY
Sbjct: 86 PVPIRWRATAIPFFVLVLFLSTISPSRAIYCD-----EDDCYDLLGVTQSANASEIKKAY 250
Query: 80 KQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRRIMYDRDMAIGIHLAFNACK 138
+L+ KYHPD +P E K F++V AYE L D + R YD +A + +N +
Sbjct: 251 YKLSLKYHPDKNPD---PESRKLFVKVANAYEILKDEATREQYDYAIAHPEEVFYNTAR 418
>TC220213 similar to UP|Q7XYV0 (Q7XYV0) P58IPK, partial (38%)
Length = 981
Score = 63.2 bits (152), Expect = 7e-11
Identities = 30/70 (42%), Positives = 47/70 (66%)
Frame = +1
Query: 55 AEERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLS 114
++ + +Y +LGI ++ S DIKRAYK+LA ++HPD + R +E +F ++ AYE LS
Sbjct: 205 SKRKDYYKILGISKTASAADIKRAYKKLALQWHPDKNVEKR-EEAEAQFREIAAAYEVLS 381
Query: 115 DPSRRIMYDR 124
D +R+ YDR
Sbjct: 382 DEDKRVRYDR 411
>TC205941 homologue to UP|Q8H2B1 (Q8H2B1) DnaJ-like protein, complete
Length = 814
Score = 62.8 bits (151), Expect = 9e-11
Identities = 31/66 (46%), Positives = 41/66 (61%), Gaps = 2/66 (3%)
Frame = +3
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPD--VSPPDRVKEYTKRFIQVQEAYETLSDPS 117
+Y +LGI S DI+ AY++LA ++HPD P E +RF Q+QEAY LSD S
Sbjct: 192 YYSVLGIRRDASFSDIRTAYRKLAMRWHPDKWAQNPATAGEAKRRFQQIQEAYSVLSDQS 371
Query: 118 RRIMYD 123
+R MYD
Sbjct: 372 KRSMYD 389
>TC222989 weakly similar to UP|DJB6_HUMAN (O75190) DnaJ homolog subfamily B
member 6 (Heat shock protein J2) (HSJ-2) (MSJ-1) (HHDJ1)
(MRJ), partial (10%)
Length = 699
Score = 62.4 bits (150), Expect = 1e-10
Identities = 31/79 (39%), Positives = 53/79 (66%), Gaps = 7/79 (8%)
Frame = +1
Query: 52 GMAAEER---SFYDLLGIPESGSLMDIKRAYKQLARKYHPD----VSPPDRVKEYTKRFI 104
GMA E + +FY +LG+ + + +++K AY++LA+K+HPD + V+E K+F
Sbjct: 325 GMANEGKKSNNFYSILGLSKECTELELKNAYRKLAKKWHPDRCSATGNLELVEEAKKKFQ 504
Query: 105 QVQEAYETLSDPSRRIMYD 123
+++EAY LSD ++R+MYD
Sbjct: 505 EIREAYSVLSDANKRLMYD 561
>TC228163 similar to UP|Q8LEU4 (Q8LEU4) DnaJ protein-like, partial (65%)
Length = 1660
Score = 62.4 bits (150), Expect = 1e-10
Identities = 32/73 (43%), Positives = 45/73 (60%)
Frame = +2
Query: 52 GMAAEERSFYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYE 111
G A+ R +YD+LG+ ++ S +IK+AY LA+K HPD + D E K+F +V AYE
Sbjct: 377 GSASLARDYYDILGVSKNASSSEIKKAYYGLAKKLHPDTNKDDPQAE--KKFQEVSIAYE 550
Query: 112 TLSDPSRRIMYDR 124
L D RR YD+
Sbjct: 551 VLKDEERRQQYDQ 589
>TC211892 similar to UP|Q9FEW7 (Q9FEW7) DnaJ like protein, partial (38%)
Length = 579
Score = 62.4 bits (150), Expect = 1e-10
Identities = 28/65 (43%), Positives = 45/65 (69%)
Frame = +2
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRR 119
+Y +L + S D+K+AY++LA K+HPD +P ++ KE +F Q+ EAYE LSDP ++
Sbjct: 203 YYKILQVDRSAKDDDLKKAYRKLAMKWHPDKNPNNK-KEAEAKFKQISEAYEVLSDPQKK 379
Query: 120 IMYDR 124
+YD+
Sbjct: 380 AIYDQ 394
>TC231504 similar to UP|Q9FEW7 (Q9FEW7) DnaJ like protein, partial (38%)
Length = 648
Score = 62.0 bits (149), Expect = 2e-10
Identities = 28/65 (43%), Positives = 46/65 (70%)
Frame = +2
Query: 60 FYDLLGIPESGSLMDIKRAYKQLARKYHPDVSPPDRVKEYTKRFIQVQEAYETLSDPSRR 119
+Y +L + + S ++KRAY++LA K+HPD +P ++ KE +F Q+ E+YE LSDP +R
Sbjct: 59 YYGILEVDRNASDEELKRAYRKLAMKWHPDKNPTNK-KEAEIQFKQISESYEVLSDPQKR 235
Query: 120 IMYDR 124
++DR
Sbjct: 236 AIFDR 250
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.134 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,979,241
Number of Sequences: 63676
Number of extensions: 94762
Number of successful extensions: 779
Number of sequences better than 10.0: 132
Number of HSP's better than 10.0 without gapping: 728
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 729
length of query: 186
length of database: 12,639,632
effective HSP length: 92
effective length of query: 94
effective length of database: 6,781,440
effective search space: 637455360
effective search space used: 637455360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146751.9