BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146743.12 + phase: 0
(139 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC203510 weakly similar to UP|Q9ZVC7 (Q9ZVC7) Expressed protein,... 161 1e-40
TC203567 UP|Q43437 (Q43437) Photosystem II type I chlorophyll a/... 159 5e-40
TC205526 weakly similar to UP|Q8VYI9 (Q8VYI9) AT5g64080/MHJ24_6,... 75 8e-15
TC227464 similar to UP|Q8VYI9 (Q8VYI9) AT5g64080/MHJ24_6, partia... 75 1e-14
TC227466 similar to UP|Q9FMI9 (Q9FMI9) Similarity to nonspecific... 74 2e-14
TC227465 similar to UP|Q6I6X1 (Q6I6X1) Xylogen protein 1, partia... 74 2e-14
TC210593 weakly similar to UP|Q9LJ86 (Q9LJ86) Gb|AAD48513.1 (AT3... 72 7e-14
TC215660 similar to UP|Q9LJ86 (Q9LJ86) Gb|AAD48513.1 (AT3g22600/... 72 1e-13
TC221444 weakly similar to UP|Q8H574 (Q8H574) Protease inhibitor... 64 3e-11
TC225985 weakly similar to UP|Q6Z387 (Q6Z387) Lipid transfer pro... 50 3e-07
TC225984 weakly similar to UP|Q6Z387 (Q6Z387) Lipid transfer pro... 49 1e-06
TC209678 weakly similar to GB|AAP12845.1|30017223|BT006196 At2g4... 48 2e-06
TC224530 46 5e-06
TC215376 similar to UP|UGP5_ARATH (Q9C7F7) Uncharacterized GPI-a... 45 1e-05
TC215375 weakly similar to UP|UGP5_ARATH (Q9C7F7) Uncharacterize... 44 3e-05
TC208229 similar to UP|Q8RW93 (Q8RW93) AT4g00170/F6N15_21, parti... 41 2e-04
TC215070 similar to UP|Q40336 (Q40336) Proline-rich cell wall pr... 41 2e-04
AW185661 homologue to GP|6164957|gb|A vacuolar H+-ATP synthase 1... 41 2e-04
TC224588 similar to UP|P93346 (P93346) NT16 polypeptide, partial... 41 2e-04
TC215069 similar to UP|O24300 (O24300) PtxA protein precursor, p... 40 3e-04
>TC203510 weakly similar to UP|Q9ZVC7 (Q9ZVC7) Expressed protein, partial
(24%)
Length = 975
Score = 161 bits (407), Expect = 1e-40
Identities = 85/131 (64%), Positives = 97/131 (73%)
Frame = +1
Query: 1 MATTTLVVAIVSLIFASLSFHGDGVSAAALAQSPAPETAVLAPSPADDGCLMALTNMSDC 60
+AT + VVA L+ +L V AQSPA AP+P +GCLMALTNMSDC
Sbjct: 136 IATNSTVVATRMLVVITLVLAL--VCCVTSAQSPAA-----APAPESNGCLMALTNMSDC 294
Query: 61 LTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTADSFGIKINVNKALKLPTIC 120
LT+VEDGSKL KPDKGCCPELAGLID NPICLC+LLG DS GIKI++NKALKLP++C
Sbjct: 295 LTYVEDGSKLAKPDKGCCPELAGLIDSNPICLCELLGK--PDSIGIKIDLNKALKLPSVC 468
Query: 121 GVTTPPVSACS 131
GVTTPPVS CS
Sbjct: 469 GVTTPPVSTCS 501
>TC203567 UP|Q43437 (Q43437) Photosystem II type I chlorophyll a/b-binding
protein precursor, partial (52%)
Length = 1448
Score = 159 bits (401), Expect = 5e-40
Identities = 72/101 (71%), Positives = 85/101 (83%)
Frame = -2
Query: 31 AQSPAPETAVLAPSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPI 90
A + P+T AP+P +GCLMAL NMSDCLT+VEDGSKL+KPDKGCCPELAGL+D NPI
Sbjct: 823 APTSVPKTTAAAPAPESNGCLMALANMSDCLTYVEDGSKLSKPDKGCCPELAGLVDSNPI 644
Query: 91 CLCKLLGSNTADSFGIKINVNKALKLPTICGVTTPPVSACS 131
CLC++LG DS GIKI++NKALKLP++CGVTTPPVS CS
Sbjct: 643 CLCEMLGK--PDSIGIKIDLNKALKLPSVCGVTTPPVSTCS 527
>TC205526 weakly similar to UP|Q8VYI9 (Q8VYI9) AT5g64080/MHJ24_6, partial
(59%)
Length = 942
Score = 75.5 bits (184), Expect = 8e-15
Identities = 45/124 (36%), Positives = 65/124 (52%)
Frame = +3
Query: 7 VVAIVSLIFASLSFHGDGVSAAALAQSPAPETAVLAPSPADDGCLMALTNMSDCLTFVED 66
VVAI ++ S S H A++PAP +V C + +SDCLTFV +
Sbjct: 111 VVAIWAVDLGSSSHH---------ARAPAPAPSV--------ECSNLVLTLSDCLTFVSN 239
Query: 67 GSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTADSFGIKINVNKALKLPTICGVTTPP 126
GS +TKP CC L +++ P CLC+ N++ G+ INV KA+ LP C ++TP
Sbjct: 240 GSTVTKPQGTCCSSLKTVLNTAPKCLCEAF--NSSAQLGLAINVTKAVTLPAACKLSTPS 413
Query: 127 VSAC 130
+ C
Sbjct: 414 AANC 425
>TC227464 similar to UP|Q8VYI9 (Q8VYI9) AT5g64080/MHJ24_6, partial (53%)
Length = 829
Score = 74.7 bits (182), Expect = 1e-14
Identities = 44/128 (34%), Positives = 70/128 (54%)
Frame = +2
Query: 3 TTTLVVAIVSLIFASLSFHGDGVSAAALAQSPAPETAVLAPSPADDGCLMALTNMSDCLT 62
+T + +++L+ A+L A++ + SP AP+P+ D + LT M+DCL+
Sbjct: 8 STRSKLTLIALVVAALCAVDLTQRASSSSSSPH------APAPSVDCSNLVLT-MADCLS 166
Query: 63 FVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTADSFGIKINVNKALKLPTICGV 122
FV +GS +TKP+ CC L ++ P CLC+ S+ FG+ +NV KA LP C V
Sbjct: 167 FVTNGSTVTKPEGTCCSGLKSVLKTAPACLCEAFKSSA--QFGVVLNVTKATTLPAACKV 340
Query: 123 TTPPVSAC 130
+ P + C
Sbjct: 341 SAPSATNC 364
>TC227466 similar to UP|Q9FMI9 (Q9FMI9) Similarity to nonspecific
lipid-transfer protein, partial (51%)
Length = 674
Score = 74.3 bits (181), Expect = 2e-14
Identities = 42/126 (33%), Positives = 68/126 (53%)
Frame = +3
Query: 5 TLVVAIVSLIFASLSFHGDGVSAAALAQSPAPETAVLAPSPADDGCLMALTNMSDCLTFV 64
TL+ ++++ ++ ++ +A A SP AP+P+ D C + M+DCL+FV
Sbjct: 66 TLIALVLAVALCAVD-----LTHSASASSPH------APAPSVD-CTNLVLTMADCLSFV 209
Query: 65 EDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTADSFGIKINVNKALKLPTICGVTT 124
+GS +TKP+ CC L ++ P CLC+ S+ FG+ +NV KA LP C V+
Sbjct: 210 TNGSTVTKPEGTCCSGLKSVLKTAPACLCEAFKSSA--QFGVVLNVTKATSLPAACKVSA 383
Query: 125 PPVSAC 130
P + C
Sbjct: 384 PSATNC 401
>TC227465 similar to UP|Q6I6X1 (Q6I6X1) Xylogen protein 1, partial (53%)
Length = 488
Score = 74.3 bits (181), Expect = 2e-14
Identities = 42/126 (33%), Positives = 68/126 (53%)
Frame = +2
Query: 5 TLVVAIVSLIFASLSFHGDGVSAAALAQSPAPETAVLAPSPADDGCLMALTNMSDCLTFV 64
TL+ ++++ ++ ++ +A A SP AP+P+ D C + M+DCL+FV
Sbjct: 59 TLIALVLAVALCAVD-----LTHSASASSPH------APAPSVD-CTNLVLTMADCLSFV 202
Query: 65 EDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTADSFGIKINVNKALKLPTICGVTT 124
+GS +TKP+ CC L ++ P CLC+ S+ FG+ +NV KA LP C V+
Sbjct: 203 TNGSTVTKPEGTCCSGLKSVLKTAPACLCEAFKSSA--QFGVVLNVTKATSLPAACKVSA 376
Query: 125 PPVSAC 130
P + C
Sbjct: 377 PSATNC 394
>TC210593 weakly similar to UP|Q9LJ86 (Q9LJ86) Gb|AAD48513.1
(AT3g22600/F16J14_17), partial (56%)
Length = 728
Score = 72.4 bits (176), Expect = 7e-14
Identities = 35/85 (41%), Positives = 47/85 (55%)
Frame = +2
Query: 46 ADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTADSFG 105
A GC LT++S CL ++ S + P CC +L+ ++ +P CLC +L +FG
Sbjct: 173 AQSGCTNTLTSLSPCLNYIMGSS--STPSASCCSQLSSIVQSSPQCLCSVLNGG-GSTFG 343
Query: 106 IKINVNKALKLPTICGVTTPPVSAC 130
I IN AL LP C V TPPVS C
Sbjct: 344 ITINQTLALSLPGACEVQTPPVSQC 418
>TC215660 similar to UP|Q9LJ86 (Q9LJ86) Gb|AAD48513.1 (AT3g22600/F16J14_17),
partial (48%)
Length = 797
Score = 71.6 bits (174), Expect = 1e-13
Identities = 35/90 (38%), Positives = 49/90 (53%)
Frame = +3
Query: 42 APSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTA 101
A + + C L ++S CL ++ S + P GCC +LA ++ P CLC++L S
Sbjct: 138 AAAQSQSSCTNVLVSLSPCLNYITGNS--STPSSGCCSQLASVVRSQPQCLCQVL-SGGG 308
Query: 102 DSFGIKINVNKALKLPTICGVTTPPVSACS 131
S GI IN +AL LP C V TPP S C+
Sbjct: 309 SSLGININQTQALALPVACKVQTPPTSQCN 398
>TC221444 weakly similar to UP|Q8H574 (Q8H574) Protease inhibitor-like
protein, partial (32%)
Length = 657
Score = 63.5 bits (153), Expect = 3e-11
Identities = 29/83 (34%), Positives = 48/83 (56%), Gaps = 1/83 (1%)
Frame = +1
Query: 50 CLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLL-GSNTADSFGIKI 108
C ++S CL +V + + + P CC +LA ++ P+CLC+++ G ++ + I
Sbjct: 169 CTNVFISLSPCLDYVTENASI--PSSSCCSQLAFVVRSQPLCLCEVVNGGASSIAASFNI 342
Query: 109 NVNKALKLPTICGVTTPPVSACS 131
N +AL LPT C V TP +S+CS
Sbjct: 343 NQTRALALPTSCNVQTPSISSCS 411
>TC225985 weakly similar to UP|Q6Z387 (Q6Z387) Lipid transfer protein-like,
partial (40%)
Length = 1027
Score = 50.4 bits (119), Expect = 3e-07
Identities = 26/75 (34%), Positives = 38/75 (50%)
Frame = +3
Query: 50 CLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTADSFGIKIN 109
C L ++ CL++V G + P CC + +I+ + CLC L+ S G+KIN
Sbjct: 171 CTDKLLGLAGCLSYV--GGEAKVPTMDCCSGIKEVINKSKRCLCILIKDRDDPSLGLKIN 344
Query: 110 VNKALKLPTICGVTT 124
V AL LP +C T
Sbjct: 345 VTLALNLPDVCETPT 389
>TC225984 weakly similar to UP|Q6Z387 (Q6Z387) Lipid transfer protein-like,
partial (28%)
Length = 1140
Score = 48.5 bits (114), Expect = 1e-06
Identities = 26/75 (34%), Positives = 36/75 (47%)
Frame = +2
Query: 50 CLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTADSFGIKIN 109
C L ++ CL +V G + P CC + +ID + CLC L+ + G+KIN
Sbjct: 239 CTDKLLGLAGCLPYV--GGEAKVPAMDCCSGIREVIDKSKRCLCILIKDRDDPNLGLKIN 412
Query: 110 VNKALKLPTICGVTT 124
V AL LP C T
Sbjct: 413 VTLALSLPDACQTPT 457
>TC209678 weakly similar to GB|AAP12845.1|30017223|BT006196 At2g44300
{Arabidopsis thaliana;} , partial (49%)
Length = 764
Score = 47.8 bits (112), Expect = 2e-06
Identities = 23/71 (32%), Positives = 37/71 (51%)
Frame = +2
Query: 50 CLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTADSFGIKIN 109
C L +++ C+ +V G + P CC L ++D + CLC L+ + GIKIN
Sbjct: 149 CADKLIDLASCVPYV--GGEAKTPTIDCCTGLKAVLDRSKKCLCILIKDRDDPNLGIKIN 322
Query: 110 VNKALKLPTIC 120
A++LP+ C
Sbjct: 323 ATLAIQLPSAC 355
>TC224530
Length = 556
Score = 46.2 bits (108), Expect = 5e-06
Identities = 31/94 (32%), Positives = 44/94 (45%), Gaps = 3/94 (3%)
Frame = +3
Query: 38 TAVLAPSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLL- 96
T + S D CL L+ + L ED P CC L +I+ + CLC L+
Sbjct: 81 TCIPRGSAQDSSCLNKLSPCLNYLNGTED------PPDSCCEPLKSVIESDAECLCSLVS 242
Query: 97 --GSNTADSFGIKINVNKALKLPTICGVTTPPVS 128
G+ A+ G IN+N+A +LP CG P+S
Sbjct: 243 NRGTRQAEQAG--ININEAQQLPGRCGQHVNPLS 338
>TC215376 similar to UP|UGP5_ARATH (Q9C7F7) Uncharacterized GPI-anchored
protein At1g27950 precursor, partial (24%)
Length = 1023
Score = 45.1 bits (105), Expect = 1e-05
Identities = 30/95 (31%), Positives = 43/95 (44%), Gaps = 8/95 (8%)
Frame = +2
Query: 44 SPADD---GCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLL---- 96
S ADD C + + CL F + K P K CC + + NP CLC ++
Sbjct: 158 SGADDLATKCSAVIQKVIPCLDFAK--GKEETPKKQCCDAATSIKESNPECLCYIIEETH 331
Query: 97 -GSNTADSFGIKINVNKALKLPTICGVTTPPVSAC 130
GS S GI+ K L+LP++C V ++ C
Sbjct: 332 KGSPQVKSLGIQ--EAKLLQLPSVCNVKNASITNC 430
>TC215375 weakly similar to UP|UGP5_ARATH (Q9C7F7) Uncharacterized
GPI-anchored protein At1g27950 precursor, partial (45%)
Length = 879
Score = 43.9 bits (102), Expect = 3e-05
Identities = 25/86 (29%), Positives = 39/86 (45%), Gaps = 5/86 (5%)
Frame = +1
Query: 50 CLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLL-----GSNTADSF 104
C + + CL F ++ P K CC + + NP CLC ++ GS S
Sbjct: 112 CSAVIQKVIPCLNFATGKEEM--PKKECCDAATAIKESNPECLCYIIQETHKGSPQVKSL 285
Query: 105 GIKINVNKALKLPTICGVTTPPVSAC 130
GI+ K L+LP++C V ++ C
Sbjct: 286 GIQ--EAKLLQLPSVCNVKNASITNC 357
>TC208229 similar to UP|Q8RW93 (Q8RW93) AT4g00170/F6N15_21, partial (66%)
Length = 597
Score = 40.8 bits (94), Expect = 2e-04
Identities = 34/115 (29%), Positives = 47/115 (40%), Gaps = 9/115 (7%)
Frame = +1
Query: 26 SAAALAQSPAPETAVLAPSPADDGCLMALTNMSDCLTFVEDGSKL--------TKPDKGC 77
S A P P + + PS + C D L F GS L TKP + C
Sbjct: 97 SCFAADNLPCPPKSTIPPSSSPQKC------PKDTLKFGVCGSWLGLVKEVIGTKPSEEC 258
Query: 78 CPELAGLID-GNPICLCKLLGSNTADSFGIKINVNKALKLPTICGVTTPPVSACS 131
C L GL D +CLC + +N + +K++V +L L CG P C+
Sbjct: 259 CILLKGLADLEAALCLCTAIKANVLGAVKVKVHVAVSL-LVNACGKKVPSGFVCA 420
>TC215070 similar to UP|Q40336 (Q40336) Proline-rich cell wall protein,
partial (82%)
Length = 1597
Score = 40.8 bits (94), Expect = 2e-04
Identities = 30/102 (29%), Positives = 43/102 (41%), Gaps = 4/102 (3%)
Frame = +2
Query: 34 PAPETAVLAPSPADDGCLMALTNMSDCLTFVEDGSKL---TKPDKGCCPELAGLID-GNP 89
P P V P PA C + + C+ + + + + CCP LAGL+D
Sbjct: 881 PPPPPVVPYPPPAQPTCPIDTLKLGACVDVLGGLIHIGIGSSAQQTCCPVLAGLVDLDAA 1060
Query: 90 ICLCKLLGSNTADSFGIKINVNKALKLPTICGVTTPPVSACS 131
+CLC + A I I + AL+L CG T P C+
Sbjct: 1061VCLCTTI---RAKILNINIIIPIALQLLIDCGKTPPDGFKCA 1177
>AW185661 homologue to GP|6164957|gb|A vacuolar H+-ATP synthase 16kDa
proteolipid subunit {Dendrobium crumenatum}, partial
(24%)
Length = 441
Score = 40.8 bits (94), Expect = 2e-04
Identities = 22/51 (43%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
Frame = +1
Query: 72 KPDKGCCPELAGLID-GNPICLCKLLGSNTADSFGIKINVNKALKLPTICG 121
KPDKGCCP +AGLID +CLC +L + I +++ L L +CG
Sbjct: 208 KPDKGCCPLIAGLIDLEAAVCLCAVLKLDLGGIISIPLDIFVNLLL-NMCG 357
>TC224588 similar to UP|P93346 (P93346) NT16 polypeptide, partial (17%)
Length = 546
Score = 40.8 bits (94), Expect = 2e-04
Identities = 22/51 (43%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
Frame = +2
Query: 72 KPDKGCCPELAGLID-GNPICLCKLLGSNTADSFGIKINVNKALKLPTICG 121
KPDKGCCP +AGLID +CLC +L + I +++ L L +CG
Sbjct: 209 KPDKGCCPLIAGLIDLEAAVCLCAVLKLDLGGIISIPLDIFVNLLL-NMCG 358
>TC215069 similar to UP|O24300 (O24300) PtxA protein precursor, partial (83%)
Length = 1405
Score = 40.4 bits (93), Expect = 3e-04
Identities = 33/105 (31%), Positives = 43/105 (40%), Gaps = 7/105 (6%)
Frame = +2
Query: 34 PAPETAVLAPSPADDGCLMALTNMSDC------LTFVEDGSKLTKPDKGCCPELAGLID- 86
P P V P PA C + + C L + GS + CCP LAGL+D
Sbjct: 707 PPPPPVVPYPPPAQPTCPIDSLKLGACVDVLGGLIHIGIGSSAKQT---CCPVLAGLVDL 877
Query: 87 GNPICLCKLLGSNTADSFGIKINVNKALKLPTICGVTTPPVSACS 131
+CLC + A I I + AL+L CG T P C+
Sbjct: 878 DAAVCLCTTI---RAKILNINIIIPIALQLLIDCGKTPPDGFKCA 1003
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.135 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,940,077
Number of Sequences: 63676
Number of extensions: 102247
Number of successful extensions: 615
Number of sequences better than 10.0: 79
Number of HSP's better than 10.0 without gapping: 602
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 604
length of query: 139
length of database: 12,639,632
effective HSP length: 87
effective length of query: 52
effective length of database: 7,099,820
effective search space: 369190640
effective search space used: 369190640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC146743.12