BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146722.13 + phase: 0
(142 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC220220 similar to UP|P74283 (P74283) Hydrogenase component, pa... 172 5e-44
TC220219 weakly similar to GB|AAP13368.1|30023670|BT006260 At3g1... 72 1e-28
TC218181 similar to GB|AAP13368.1|30023670|BT006260 At3g16240 {A... 83 4e-17
TC218182 similar to GB|AAP13368.1|30023670|BT006260 At3g16240 {A... 60 5e-10
CD410982 45 2e-05
BG881763 31 0.23
TC210062 GT-2 factor [Glycine max] 30 0.38
TC231953 30 0.38
TC226922 similar to UP|Q9LPQ3 (Q9LPQ3) F15H18.14, partial (54%) 30 0.50
BU764891 29 0.66
TC220939 similar to UP|Q75W15 (Q75W15) 3-deoxy-D-arabino heptulo... 28 1.1
CB063410 GP|13274996|em soybean protox-1 {Glycine max}, partial ... 28 1.1
TC219257 weakly similar to UP|Q6PLP6 (Q6PLP6) Cell wall protein ... 28 1.5
TC222647 weakly similar to UP|O24341 (O24341) UDPG glucosyltrans... 28 1.9
AW156172 28 1.9
AW705627 weakly similar to GP|30017239|gb| At5g49350 {Arabidopsi... 27 2.5
TC216398 homologue to UP|Q6NPD6 (Q6NPD6) At2g22125, partial (60%) 27 2.5
BU544265 27 2.5
BI784652 27 2.5
TC211919 weakly similar to UP|K2C1_HUMAN (P04264) Keratin, type ... 27 3.3
>TC220220 similar to UP|P74283 (P74283) Hydrogenase component, partial (19%)
Length = 751
Score = 172 bits (436), Expect = 5e-44
Identities = 93/143 (65%), Positives = 103/143 (71%), Gaps = 1/143 (0%)
Frame = +1
Query: 1 MACMNNIFTPFPLTAGNRNPTIYFPRRR-PNSVCLVANSPESPSPPPEIELEFVGPKPEG 59
MA M+ IFT L A RNP IYFPRRR PN+V A S SP PPEIELEF+GPKP
Sbjct: 121 MASMS-IFTTISLAADARNPAIYFPRRRQPNTVIFAAAS--SPESPPEIELEFIGPKPGS 291
Query: 60 DGSFPVDKVKAISGEKLMRSIMSDNKIDLYATYGKLMNCGGGGSCGTCIVEKPESWRLAC 119
DGS+PV++VKAISGEKL+R+IM DNKI+LYATYGKLMNC GGGSCGTCIVE E L
Sbjct: 292 DGSYPVERVKAISGEKLLRNIMLDNKIELYATYGKLMNCAGGGSCGTCIVEIIEGKDLLN 471
Query: 120 QTIVGNKENSGKVVVQRIPQWKK 142
+ KV VQRIPQWKK
Sbjct: 472 ERTNTELRYLSKVAVQRIPQWKK 540
>TC220219 weakly similar to GB|AAP13368.1|30023670|BT006260 At3g16240
{Arabidopsis thaliana;} , partial (21%)
Length = 588
Score = 72.0 bits (175), Expect(2) = 1e-28
Identities = 32/33 (96%), Positives = 33/33 (99%)
Frame = +1
Query: 110 EKPESWRLACQTIVGNKENSGKVVVQRIPQWKK 142
+KPESWRLACQTIVGNKENSGKVVVQRIPQWKK
Sbjct: 166 KKPESWRLACQTIVGNKENSGKVVVQRIPQWKK 264
Score = 70.1 bits (170), Expect(2) = 1e-28
Identities = 30/34 (88%), Positives = 33/34 (96%)
Frame = +3
Query: 77 MRSIMSDNKIDLYATYGKLMNCGGGGSCGTCIVE 110
+R+IM DNKI+LYATYGKLMNCGGGGSCGTCIVE
Sbjct: 3 LRNIMLDNKIELYATYGKLMNCGGGGSCGTCIVE 104
>TC218181 similar to GB|AAP13368.1|30023670|BT006260 At3g16240 {Arabidopsis
thaliana;} , partial (56%)
Length = 856
Score = 83.2 bits (204), Expect = 4e-17
Identities = 48/123 (39%), Positives = 65/123 (52%), Gaps = 23/123 (18%)
Frame = +3
Query: 42 PSPPPEIELEFVGPKPEGDGSFPVDKVKAISGEKLMRSIMSDNKIDLYATYGK-LMNCGG 100
P PP + L FV DG+ + A G++L R IM D+ +DLY Y + L+NCGG
Sbjct: 222 PDEPPYVGLVFVSSVLLPDGTPDMHFRSACGGQRL-RKIMLDSNVDLYGPYARPLLNCGG 398
Query: 101 GGSCGTCIVE----------------------KPESWRLACQTIVGNKENSGKVVVQRIP 138
GG+CGTC+VE P LACQTIVG ++ G VV+Q++P
Sbjct: 399 GGTCGTCMVEVLEGKELLNPRTDKEKKISKDRNPRIG*LACQTIVGKPDSRGAVVIQQLP 578
Query: 139 QWK 141
+WK
Sbjct: 579 EWK 587
>TC218182 similar to GB|AAP13368.1|30023670|BT006260 At3g16240 {Arabidopsis
thaliana;} , partial (34%)
Length = 465
Score = 59.7 bits (143), Expect = 5e-10
Identities = 31/70 (44%), Positives = 43/70 (61%), Gaps = 1/70 (1%)
Frame = +3
Query: 42 PSPPPEIELEFVGPKPEGDGSFPVDKVKAISGEKLMRSIMSDNKIDLYATYGK-LMNCGG 100
P PP + L FV DG+ + A G++L R IM D+ ++LY Y + L+NCGG
Sbjct: 252 PDEPPYVGLVFVSSVLLPDGTPDMHFRSACGGQRL-RKIMLDSNVELYGPYARPLLNCGG 428
Query: 101 GGSCGTCIVE 110
GG+CGTC+VE
Sbjct: 429 GGTCGTCMVE 458
>CD410982
Length = 375
Score = 44.7 bits (104), Expect = 2e-05
Identities = 16/29 (55%), Positives = 24/29 (82%)
Frame = -1
Query: 113 ESWRLACQTIVGNKENSGKVVVQRIPQWK 141
++WRLACQT VG ++ G VV+Q++P+WK
Sbjct: 366 KNWRLACQTTVGKPDSRGAVVIQQLPEWK 280
>BG881763
Length = 392
Score = 30.8 bits (68), Expect = 0.23
Identities = 21/60 (35%), Positives = 25/60 (41%), Gaps = 4/60 (6%)
Frame = -1
Query: 9 TPFPLTAGNRNPTI-YFPRRRPNSVCLVANSPESPSPP---PEIELEFVGPKPEGDGSFP 64
TP P +AG P P P S+ ++ SPE PSPP P P P FP
Sbjct: 227 TPQPRSAGTLAPPC*PSPPSPPTSISVLLRSPEPPSPPASEPPPPTRR*TPSPPSTADFP 48
>TC210062 GT-2 factor [Glycine max]
Length = 712
Score = 30.0 bits (66), Expect = 0.38
Identities = 13/28 (46%), Positives = 15/28 (53%)
Frame = +3
Query: 19 NPTIYFPRRRPNSVCLVANSPESPSPPP 46
NPT YFP + PN N+P S PP
Sbjct: 147 NPTTYFPTQTPNPTSNNNNNPPSTITPP 230
>TC231953
Length = 674
Score = 30.0 bits (66), Expect = 0.38
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +3
Query: 10 PFPLTAGNRNPTIYFPRRRPNSVCLVANSPESPS 43
PFP A + P++ P R PNS+C + +PS
Sbjct: 474 PFP*RAASMVPSVPLPWRNPNSICRKSKQGLAPS 575
>TC226922 similar to UP|Q9LPQ3 (Q9LPQ3) F15H18.14, partial (54%)
Length = 1190
Score = 29.6 bits (65), Expect = 0.50
Identities = 14/36 (38%), Positives = 17/36 (46%)
Frame = +2
Query: 11 FPLTAGNRNPTIYFPRRRPNSVCLVANSPESPSPPP 46
+P +A PT P R P S + SP P PPP
Sbjct: 74 WPSSATAATPTSVSPSRSPPSAASASPSPSLPPPPP 181
>BU764891
Length = 421
Score = 29.3 bits (64), Expect = 0.66
Identities = 15/36 (41%), Positives = 21/36 (57%), Gaps = 3/36 (8%)
Frame = +2
Query: 25 PRRRPNSVCLVANSPESP---SPPPEIELEFVGPKP 57
PRR + L N PE P +PPP+ E++ GP+P
Sbjct: 20 PRRSRLFLFLRMNPPEIPFGGAPPPKKEVQLQGPRP 127
>TC220939 similar to UP|Q75W15 (Q75W15) 3-deoxy-D-arabino
heptulosonate-7-phosphate synthase, partial (13%)
Length = 442
Score = 28.5 bits (62), Expect = 1.1
Identities = 18/58 (31%), Positives = 25/58 (43%), Gaps = 4/58 (6%)
Frame = +3
Query: 11 FPLTAGNRNPTIYFPRRRPNSVCLVAN----SPESPSPPPEIELEFVGPKPEGDGSFP 64
FP + NPT FP P S +A+ SP +P PP+ P P+ + P
Sbjct: 84 FPPNL*SPNPTPSFPTSGPGSGPSLAHPLPSSPSTPPSPPKTPSSPTSPSPKPNNLHP 257
>CB063410 GP|13274996|em soybean protox-1 {Glycine max}, partial (18%)
Length = 386
Score = 28.5 bits (62), Expect = 1.1
Identities = 12/29 (41%), Positives = 18/29 (61%)
Frame = +3
Query: 19 NPTIYFPRRRPNSVCLVANSPESPSPPPE 47
+PT FPR RPN + + + ES + PP+
Sbjct: 177 SPTRKFPRSRPNPILRCSIAEESTASPPK 263
>TC219257 weakly similar to UP|Q6PLP6 (Q6PLP6) Cell wall protein GP2
(Fragment), partial (4%)
Length = 933
Score = 28.1 bits (61), Expect = 1.5
Identities = 16/50 (32%), Positives = 19/50 (38%)
Frame = +2
Query: 25 PRRRPNSVCLVANSPESPSPPPEIELEFVGPKPEGDGSFPVDKVKAISGE 74
PRRR NSP SP+PP ++ P G D GE
Sbjct: 233 PRRRRPRPTPSPNSPPSPTPPSPTKIPSSSPPAPASGGADSDNWNTKKGE 382
>TC222647 weakly similar to UP|O24341 (O24341) UDPG glucosyltransferase,
partial (9%)
Length = 773
Score = 27.7 bits (60), Expect = 1.9
Identities = 23/76 (30%), Positives = 33/76 (43%), Gaps = 3/76 (3%)
Frame = +3
Query: 48 IELEFVGPKPEGDGSFPVDKVKAISGEKLMRSIMSDNKIDLYATYGK---LMNCGGGGSC 104
I + +G PE G D VK K ++ +MS+ + + A K L G GGSC
Sbjct: 264 IRMSGMGIWPEEWGWGTQDVVKGDEIAKRIKEMMSNESLRVKAGELKEAALKAAGVGGSC 443
Query: 105 GTCIVEKPESWRLACQ 120
I + E W+ Q
Sbjct: 444 EVTIKRQIEEWKRNAQ 491
>AW156172
Length = 306
Score = 27.7 bits (60), Expect = 1.9
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = +3
Query: 40 ESPSPPPEIELEFVGPKPEGDGSFP 64
E P+PPP + F G KP G G P
Sbjct: 75 EKPTPPPPKKFFFGGKKPWGGGKKP 149
>AW705627 weakly similar to GP|30017239|gb| At5g49350 {Arabidopsis thaliana},
partial (10%)
Length = 391
Score = 27.3 bits (59), Expect = 2.5
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +2
Query: 27 RRPNSVCLVANSPESPSPPPEIELEFVGPKPEGDGSFPVDKV 68
R PN + + + P++ PPP + + V P PE D P + V
Sbjct: 119 RNPNQIPEIPSVPKTEPPPPVPKEQPVLPTPEIDPFMPPEIV 244
>TC216398 homologue to UP|Q6NPD6 (Q6NPD6) At2g22125, partial (60%)
Length = 859
Score = 27.3 bits (59), Expect = 2.5
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +1
Query: 43 SPPPEIELEFVGPKPEGDGSFPVDKVKAISGEKLMRSIMSDNKIDLYATYGKL 95
+PP + ++ GP PE D SF G+KL S + +K+ +++GK+
Sbjct: 280 TPPRQTKVVSTGPNPEWDESFTWSFESPPKGQKLHISCKNKSKMG-KSSFGKV 435
>BU544265
Length = 390
Score = 27.3 bits (59), Expect = 2.5
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +1
Query: 109 VEKPESWRLACQTIVGNKE 127
+ +P SW+LAC +VG K+
Sbjct: 181 IHEPLSWKLACANLVGTKK 237
>BI784652
Length = 421
Score = 27.3 bits (59), Expect = 2.5
Identities = 14/37 (37%), Positives = 18/37 (47%)
Frame = +1
Query: 25 PRRRPNSVCLVANSPESPSPPPEIELEFVGPKPEGDG 61
PRR P+ L P P+P P L+ GP+ G G
Sbjct: 241 PRRHPSQAPL---RPRGPAPGPGPRLQVQGPRQGGPG 342
>TC211919 weakly similar to UP|K2C1_HUMAN (P04264) Keratin, type II
cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa
cytokeratin) (Hair alpha protein), partial (5%)
Length = 590
Score = 26.9 bits (58), Expect = 3.3
Identities = 18/55 (32%), Positives = 26/55 (46%), Gaps = 2/55 (3%)
Frame = +3
Query: 6 NIFTPFPLTAGNRNPTIYFPRRRPNSV--CLVANSPESPSPPPEIELEFVGPKPE 58
N TP P + + NPT + PN + +N+P P PPP ++ P PE
Sbjct: 243 NFNTPTPPSPIDNNPTTPQTPQLPNPIPPPPYSNNPSLPPPPPILQSP---PPPE 398
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.137 0.430
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,077,682
Number of Sequences: 63676
Number of extensions: 141207
Number of successful extensions: 1527
Number of sequences better than 10.0: 85
Number of HSP's better than 10.0 without gapping: 1267
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1479
length of query: 142
length of database: 12,639,632
effective HSP length: 88
effective length of query: 54
effective length of database: 7,036,144
effective search space: 379951776
effective search space used: 379951776
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)
Medicago: description of AC146722.13