Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC146719.6 - phase: 0 /pseudo
         (312 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC234828                                                               46  2e-05
CF922488                                                               29  2.4
BG652347                                                               28  5.3
TC211687 similar to UP|Q7VNN2 (Q7VNN2) Conserved probable membra...    28  7.0
BE659070 homologue to SP|O17449|TBB1 Tubulin beta-1 chain (Beta-...    27  9.1
BU765729                                                               27  9.1

>TC234828 
          Length = 857

 Score = 46.2 bits (108), Expect = 2e-05
 Identities = 34/85 (40%), Positives = 40/85 (47%)
 Frame = +1

Query: 57  CLLRIKQ*LISYRLYTIFQKFFLMKFPMCLQREKLNF*LILFLERSQCL*HLIVCQLLS* 116
           C+ +    L+ YR    FQKFF M F  C  REK N  L   L R QC   LI C   +*
Sbjct: 598 CMWKRTLKLVVYRWCQNFQKFFRMMFVNCHLREKWNSLLTWCLGRIQCRLRLIECLRWN* 777

Query: 117 LS*RNN*RICFRKSLLEQVFHLGER 141
             *R+  R     +L  Q  H GER
Sbjct: 778 QR*RHKYRTF*ANNLFVQAHHRGER 852


>CF922488 
          Length = 741

 Score = 29.3 bits (64), Expect = 2.4
 Identities = 25/78 (32%), Positives = 41/78 (52%)
 Frame = +1

Query: 148 RKMVA*DCALIIDS*TK*RERIGIHFQE*MT*WISWWVHVCSARYI*DRVITRLK*KMKI 207
           ++M   +CA  I+ *TK  +RI   ++     WI+  V   S  ++  +VITR +*  +I
Sbjct: 10  KRMGRCECAWTIEI*TKPVQRISFLYRISTFSWITRPVFPNSPSWMDFQVITR*R*HQRI 189

Query: 208 CRRQLLERGMHIMSIKLC 225
            +RQL        +I+LC
Sbjct: 190 WKRQLSLLYGEPSAIRLC 243


>BG652347 
          Length = 356

 Score = 28.1 bits (61), Expect = 5.3
 Identities = 13/48 (27%), Positives = 22/48 (45%), Gaps = 2/48 (4%)
 Frame = +1

Query: 214 ERGMHIMSIKLCLSVLQMHLVCLWST*IA--FFMHFWTVSWLFLLMTF 259
           E  M + + KLC    + H +C+     +   + H+W   W FL + F
Sbjct: 7   ETSMGLSNQKLCSDSKRSHTICICCMLSS*TIYNHYWL*GWFFLHLAF 150


>TC211687 similar to UP|Q7VNN2 (Q7VNN2) Conserved probable membrane protein,
           partial (5%)
          Length = 632

 Score = 27.7 bits (60), Expect = 7.0
 Identities = 14/36 (38%), Positives = 20/36 (54%), Gaps = 3/36 (8%)
 Frame = -1

Query: 50  YFL*WHPCLLRIKQ*LISYRLYTIFQK---FFLMKF 82
           YF+ W  C+L     L+S+ LY++F     FFL  F
Sbjct: 410 YFMLWVRCILCASLCLLSFSLYSVFLHCACFFLFSF 303


>BE659070 homologue to SP|O17449|TBB1 Tubulin beta-1 chain (Beta-1 tubulin).
           [Tobacco hawkmoth  Tobacco hornworm] {Manduca sexta},
           partial (30%)
          Length = 705

 Score = 27.3 bits (59), Expect = 9.1
 Identities = 11/20 (55%), Positives = 15/20 (75%)
 Frame = +1

Query: 275 WFCKCSKRRSFMLNYLSVNS 294
           W+C  S+ +SFML+  SVNS
Sbjct: 346 WYCWYSETKSFMLDSASVNS 405


>BU765729 
          Length = 425

 Score = 27.3 bits (59), Expect = 9.1
 Identities = 14/44 (31%), Positives = 22/44 (49%), Gaps = 4/44 (9%)
 Frame = +2

Query: 225 CLSVLQMHLVCLWS----T*IAFFMHFWTVSWLFLLMTF*FTPR 264
           CL ++     CL+     T + FF      SW+FLL+ F ++ R
Sbjct: 134 CLRIMNNTCSCLYQRSCFTGVFFFFFLQIFSWVFLLLVFMWSER 265


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.378    0.167    0.656 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,894,817
Number of Sequences: 63676
Number of extensions: 257442
Number of successful extensions: 5227
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 2920
Number of HSP's successfully gapped in prelim test: 197
Number of HSP's that attempted gapping in prelim test: 2226
Number of HSP's gapped (non-prelim): 3252
length of query: 312
length of database: 12,639,632
effective HSP length: 97
effective length of query: 215
effective length of database: 6,463,060
effective search space: 1389557900
effective search space used: 1389557900
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 13 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 35 (21.7 bits)
S2: 59 (27.3 bits)


Medicago: description of AC146719.6