BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146709.12 + phase: 0 /pseudo
(609 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC207975 similar to UP|Q93Z90 (Q93Z90) AT4g24430/T22A6_260, part... 298 6e-81
TC208177 similar to UP|Q93Z90 (Q93Z90) AT4g24430/T22A6_260, part... 229 3e-60
TC215981 similar to UP|Q93Z90 (Q93Z90) AT4g24430/T22A6_260, part... 145 5e-35
TC221431 weakly similar to UP|Q9SZK3 (Q9SZK3) LG127/30 like gene... 109 4e-24
TC215982 weakly similar to UP|Q6NKW8 (Q6NKW8) At2g22620, partial... 103 2e-22
AI973706 89 4e-18
BM891378 77 3e-14
BQ630617 49 8e-06
BI700921 similar to GP|16323222|gb| AT4g24430/T22A6_260 {Arabido... 39 0.007
TC206303 similar to GB|AAP03025.1|29893233|AY250842 acyl-activat... 30 3.1
TC209639 30 4.1
TC218852 similar to GB|AAP37807.1|30725570|BT008448 At2g29660 {A... 30 4.1
BE190579 29 6.9
>TC207975 similar to UP|Q93Z90 (Q93Z90) AT4g24430/T22A6_260, partial (49%)
Length = 1009
Score = 298 bits (762), Expect = 6e-81
Identities = 145/259 (55%), Positives = 182/259 (69%), Gaps = 3/259 (1%)
Frame = +2
Query: 354 FWTQTDAKGYFKITNVVPGHYNLFGWVSGFIGDYKYNSTITITPGGVIKLNSLVYNPPRN 413
FW + D GYF ++N+ G YN++ WV GFIGDYKY+ I IT G I L LVY PPR+
Sbjct: 2 FWARADDDGYFSVSNIRAGDYNVYAWVPGFIGDYKYDVVINITEGCDIDLGDLVYEPPRD 181
Query: 414 GPTIWEIGIPDRLTSEFHVPEPYPTLMNKLYTEGRDK--QYGLWERYTEMYPTDDLIYTL 471
GPT+WEIGIPDR +EF+VP+P P +NKLY DK QYGLWERY E+YP DLIYT+
Sbjct: 182 GPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGLWERYAELYPDKDLIYTI 361
Query: 472 GVNK-NKDWFYAHVTRNTENNTYEPTTWQIIFEHQHDLKSGNYTLQLALASAADAYLQVR 530
GV+ KDWF+A VTR ++NTY+ TTWQI F+ KS +Y L++ALASA + LQVR
Sbjct: 362 GVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNKSSSYKLRVALASATLSELQVR 541
Query: 531 FNDRSVYPPHFATGHIGRIRGDNCIQRHGIHGLYRLFSIDVPSNLLLKGKNIIYLTQTNA 590
N+ P F++G IGR DN I RHGIHGLY L+++ +P L++G N I+LTQT
Sbjct: 542 VNNPKAPRPLFSSGLIGR---DNSIARHGIHGLYWLYNVGIPGTQLVEGDNTIFLTQTRG 712
Query: 591 DTPFQGVMYDYIRLEQPPS 609
+ PFQ +MYDYIRLE PPS
Sbjct: 713 NGPFQAIMYDYIRLEAPPS 769
>TC208177 similar to UP|Q93Z90 (Q93Z90) AT4g24430/T22A6_260, partial (59%)
Length = 1304
Score = 229 bits (583), Expect = 3e-60
Identities = 112/214 (52%), Positives = 146/214 (67%), Gaps = 3/214 (1%)
Frame = +3
Query: 398 GGVIKLNSLVYNPPRNGPTIWEIGIPDRLTSEFHVPEPYPTLMNKLYTEGRDK--QYGLW 455
G I+L LVY PPR+GPT+WEIGIPDR +EF+ P+P P +NKL+ D+ QYGLW
Sbjct: 501 GSYIELGELVYEPPRDGPTLWEIGIPDRSAAEFYAPDPSPQHINKLFINHPDRFRQYGLW 680
Query: 456 ERYTEMYPTDDLIYTLGVNK-NKDWFYAHVTRNTENNTYEPTTWQIIFEHQHDLKSGNYT 514
+RY+E+YP DL+YT+GV+ KDWFYA R +NT + TTWQI FE +K Y
Sbjct: 681 DRYSELYPDADLVYTIGVSDYTKDWFYAQAPRKKVDNTLQGTTWQIKFEICSVVKGSTYK 860
Query: 515 LQLALASAADAYLQVRFNDRSVYPPHFATGHIGRIRGDNCIQRHGIHGLYRLFSIDVPSN 574
L++A+ASA A LQ+R ND + P F +G IGR DN I RHGI G+Y L+ +++P +
Sbjct: 861 LRVAIASATLAELQIRVNDPNARRPVFTSGLIGR---DNSIARHGIQGIYWLYHVNIPGS 1031
Query: 575 LLLKGKNIIYLTQTNADTPFQGVMYDYIRLEQPP 608
LL+ G N IY +Q +PFQG+MYDYIRLE PP
Sbjct: 1032LLVDGTNTIYFSQPRCTSPFQGIMYDYIRLEGPP 1133
Score = 127 bits (319), Expect(2) = 9e-34
Identities = 63/115 (54%), Positives = 81/115 (69%)
Frame = +1
Query: 236 THYAGKEVTMAFQEGETYKKVFGPVFVYLNTASSENDNATLWSDAVQQLSKEVQSWPYDF 295
+HYAG+++ F+ GE++KKVFGPV++YLN+ + ++ LW DA Q+ EVQSWPY F
Sbjct: 160 SHYAGQDLVPKFRGGESWKKVFGPVYIYLNSGAVGDNPLWLWEDAKIQMMNEVQSWPYSF 339
Query: 296 PQSQDYFPPNQRGAVFGRLLVQDWYFEGGRYQYANNAYVGLALPGDAGSWQRESK 350
P S+D+ +QRG V GRLLV D Y AN+AYVGLA PGDAGSWQRE K
Sbjct: 340 PASEDFLKSDQRGNVSGRLLVLDRYVCTDLIP-ANSAYVGLAPPGDAGSWQRECK 501
Score = 35.0 bits (79), Expect(2) = 9e-34
Identities = 15/30 (50%), Positives = 21/30 (70%)
Frame = +2
Query: 195 GLVLILIHQWVSG**HRVMSFAMVDPLSKI 224
G+ + +HQW SG H VMSF +VDP ++I
Sbjct: 26 GVDIFQVHQWDSGRSHLVMSFVLVDPSNRI 115
>TC215981 similar to UP|Q93Z90 (Q93Z90) AT4g24430/T22A6_260, partial (22%)
Length = 425
Score = 145 bits (366), Expect = 5e-35
Identities = 72/142 (50%), Positives = 96/142 (66%), Gaps = 3/142 (2%)
Frame = +3
Query: 428 SEFHVPEPYPTLMNKLYTEGRDK--QYGLWERYTEMYPTDDLIYTLGVNK-NKDWFYAHV 484
+EF+VP+P P +N+LY D+ QYGLWERY E+YP +DL+YT+G++ KDWF+A V
Sbjct: 9 AEFYVPDPNPMCINRLYVNHPDRFRQYGLWERYAELYPNEDLVYTVGISDYRKDWFFAQV 188
Query: 485 TRNTENNTYEPTTWQIIFEHQHDLKSGNYTLQLALASAADAYLQVRFNDRSVYPPHFATG 544
R +N TY TTWQI F+ +G YTLQ+ALAS A L++R N+ PP F++G
Sbjct: 189 NRKKDNGTYIGTTWQINFKLDSVNTNGTYTLQVALASVHSAELEIRINNLEANPPLFSSG 368
Query: 545 HIGRIRGDNCIQRHGIHGLYRL 566
IG+ +N I RHGIHGLY L
Sbjct: 369 VIGK---ENTIARHGIHGLYWL 425
>TC221431 weakly similar to UP|Q9SZK3 (Q9SZK3) LG127/30 like gene, partial
(13%)
Length = 532
Score = 109 bits (272), Expect = 4e-24
Identities = 64/101 (63%), Positives = 69/101 (67%), Gaps = 3/101 (2%)
Frame = +3
Query: 510 SGNYTLQLALASAADAYLQVRFNDRSVYPPHFATGHIGRIRGDNCIQRHGIHGLYRLFSI 569
S Y L+LALASA + LQVR ND PP F TG IG+ DN I RHGIHGLY LFSI
Sbjct: 9 SNIYRLRLALASANISELQVRVNDPKQDPPLFTTGVIGK---DNAIARHGIHGLYWLFSI 179
Query: 570 DVPSNLLLKGKNIIYLTQTNADTP---FQGVMYDYIRLEQP 607
DVP LL K NII+LTQT A P FQG+MYDYIRLE P
Sbjct: 180 DVPGLLLEKSVNIIFLTQTMASGPLALFQGIMYDYIRLEGP 302
>TC215982 weakly similar to UP|Q6NKW8 (Q6NKW8) At2g22620, partial (13%)
Length = 493
Score = 103 bits (257), Expect = 2e-22
Identities = 53/113 (46%), Positives = 73/113 (63%), Gaps = 1/113 (0%)
Frame = -1
Query: 448 RDKQYGLWERYTEMYPTDDLIYTLGVNK-NKDWFYAHVTRNTENNTYEPTTWQIIFEHQH 506
R +QYGLWERY E+YP +DL+YT+G++ KDWF+A V R +N T TTWQI F+
Sbjct: 343 RFRQYGLWERYAELYPNEDLVYTVGISDYRKDWFFAQVNRKKDNGTDIGTTWQINFKLDS 164
Query: 507 DLKSGNYTLQLALASAADAYLQVRFNDRSVYPPHFATGHIGRIRGDNCIQRHG 559
+ YTL++ALAS A L++R N+ PP F++G IG +G+ RHG
Sbjct: 163 VNTN*TYTLRVALASVHSAELEIRINNLEANPPLFSSGVIG--KGEIL*LRHG 11
>AI973706
Length = 208
Score = 89.4 bits (220), Expect = 4e-18
Identities = 38/66 (57%), Positives = 47/66 (70%)
Frame = +2
Query: 352 YQFWTQTDAKGYFKITNVVPGHYNLFGWVSGFIGDYKYNSTITITPGGVIKLNSLVYNPP 411
YQFWT+ D G+F I NV PG YNLF WV GF GDYK+ + IT G I+L+ L Y+PP
Sbjct: 11 YQFWTRADENGFFTIKNVRPGDYNLFAWVPGFHGDYKFGDCMKITSGSYIELSELGYDPP 190
Query: 412 RNGPTI 417
R+GPT+
Sbjct: 191 RDGPTL 208
>BM891378
Length = 319
Score = 76.6 bits (187), Expect = 3e-14
Identities = 37/59 (62%), Positives = 43/59 (72%)
Frame = +3
Query: 547 GRIRGDNCIQRHGIHGLYRLFSIDVPSNLLLKGKNIIYLTQTNADTPFQGVMYDYIRLE 605
G I +N I RHGIHGLY LFSIDV LL++G N I+LTQT +P G+MYDYIRLE
Sbjct: 42 GLIGKENTIARHGIHGLYWLFSIDVQGTLLVQGNNTIFLTQTRDTSPLIGIMYDYIRLE 218
>BQ630617
Length = 425
Score = 48.5 bits (114), Expect = 8e-06
Identities = 19/39 (48%), Positives = 28/39 (71%)
Frame = +2
Query: 1 MARPEGYILGISYNGIDNVLDSENQELDRGYFDVVWNEP 39
++ P G + GI YN IDN+L+ N E +RGY+D+VW+ P
Sbjct: 293 LSNPGGIVTGIQYNDIDNLLEVLNDESNRGYWDLVWSSP 409
>BI700921 similar to GP|16323222|gb| AT4g24430/T22A6_260 {Arabidopsis
thaliana}, partial (5%)
Length = 426
Score = 38.9 bits (89), Expect = 0.007
Identities = 18/37 (48%), Positives = 22/37 (58%), Gaps = 2/37 (5%)
Frame = +3
Query: 429 EFHVPEPYPTLMNKLYTEGRDK--QYGLWERYTEMYP 463
++H +NKLY DK QYGLWERY E+YP
Sbjct: 312 KWHTKSHLIKYINKLYVNHPDKFRQYGLWERYAELYP 422
>TC206303 similar to GB|AAP03025.1|29893233|AY250842 acyl-activating enzyme
13 {Arabidopsis thaliana;} , partial (77%)
Length = 1976
Score = 30.0 bits (66), Expect = 3.1
Identities = 24/87 (27%), Positives = 36/87 (40%), Gaps = 10/87 (11%)
Frame = +3
Query: 389 YNSTITITPGGVIKLNSLVYNPPRNGPTIWEIGIPDRLTSEF---HVPEPYPTLMNKLYT 445
Y S T P GV+ + + + + WE D+ HV + LM LY
Sbjct: 705 YTSGTTGKPKGVVHTHRSIISQVQTLTKAWEYTSADQFLHCLPLHHVHGLFNGLMAPLYA 884
Query: 446 EGRDKQY-------GLWERYTEMYPTD 465
G ++ G+W+R+ E YPTD
Sbjct: 885 -GSTVEFLPKFSVRGVWQRWRESYPTD 962
>TC209639
Length = 1451
Score = 29.6 bits (65), Expect = 4.1
Identities = 10/38 (26%), Positives = 25/38 (65%)
Frame = -3
Query: 47 STFQRIHGTNFSVIAADENMVEVSFSRLWTHSMSGKNV 84
S +++H T F+ ++++ +V+ S++RLW + G ++
Sbjct: 990 SVHKKVHHTRFNFLSSNSFLVD*SYARLWYAKLGGHHL 877
>TC218852 similar to GB|AAP37807.1|30725570|BT008448 At2g29660 {Arabidopsis
thaliana;} , partial (54%)
Length = 1201
Score = 29.6 bits (65), Expect = 4.1
Identities = 25/101 (24%), Positives = 46/101 (44%)
Frame = +1
Query: 99 SGFYSYAIFNRPKGMPGIEVDQIRFVFKLDKDRFKYMAISDTRQRNMPAMKDRNTGQVLA 158
S F+++ I RP + RFV ++ + +++ + + QR++P R
Sbjct: 154 SKFFNFLIATRPG*------ESTRFVPRI-RHLYRHQPLLPSPQRHLPLHCVRRD----- 297
Query: 159 YPEAVLLTNPINPQFRGEVDDKYQYSCENKDTQFMDGLVLI 199
+PEA L +P NPQ RG + + +++ GL I
Sbjct: 298 FPEAPPLRSPSNPQTRGFGSTRIRPGSQHRPNHLQIGLARI 420
>BE190579
Length = 444
Score = 28.9 bits (63), Expect = 6.9
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -2
Query: 533 DRSVYPPHFATGHIGRIRGDNCIQRHGIHGLYR 565
DR+ P H A GH+ R GD+ G+H + R
Sbjct: 122 DRADSPDHLAGGHVPRGAGDHPAAGGGLHAVLR 24
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.140 0.436
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,716,503
Number of Sequences: 63676
Number of extensions: 398791
Number of successful extensions: 1565
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1544
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1552
length of query: 609
length of database: 12,639,632
effective HSP length: 103
effective length of query: 506
effective length of database: 6,081,004
effective search space: 3076988024
effective search space used: 3076988024
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
Medicago: description of AC146709.12