BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146708.4 + phase: 0
(298 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC211008 76 2e-14
TC222348 56 4e-13
AW277722 similar to SP|O65719|HS73_ Heat shock cognate 70 kDa pr... 54 9e-08
TC203464 similar to GB|AAF35920.1|7025820|AC005892 L505.8 {Leish... 51 7e-07
BU547962 50 9e-07
TC208902 47 1e-05
TC213599 homologue to UP|EXBB_PASHA (P72202) Biopolymer transpor... 36 1e-04
TC220201 similar to UP|Q9ERI5 (Q9ERI5) Apoptotic cell clearance ... 43 2e-04
BQ253293 42 3e-04
CD393541 41 6e-04
CD416211 40 0.001
TC233956 38 0.006
CD396188 weakly similar to GP|6175788|gb|A ORF144 {Xestia c-nigr... 37 0.014
BI702137 35 0.031
TC222557 35 0.031
AW832520 35 0.031
BI973267 35 0.041
TC234500 35 0.041
BU579105 similar to GP|12328468|dbj P0416D03.16 {Oryza sativa (j... 35 0.053
BQ629929 35 0.053
>TC211008
Length = 516
Score = 76.3 bits (186), Expect = 2e-14
Identities = 53/147 (36%), Positives = 72/147 (48%), Gaps = 17/147 (11%)
Frame = +1
Query: 138 WKPPSAPWVKVNTDGSVLNN------SGACGGLFRDHLGTFLGAFVGNLGRCSVFDTEVS 191
W+ P WV +NTDGSV N ACGGL RD G +LG F NLG SV E+
Sbjct: 64 WRCPXNGWVCLNTDGSVFENHRNGCSGSACGGLVRDSSGCYLGGFTVNLGNTSVTLAELW 243
Query: 192 GFILVMEHAALHGWYNIWLESDASSAL------MVFKNPSLVPI-----LLRNRWHNART 240
G + ++ A G + ++ D+ +AL V +P+ + L+R W
Sbjct: 244 GVVHGLKLAWDLGCKKVKVDIDSGNALGLVRHGPVANDPAFALVSEINELVRKEW----- 408
Query: 241 LNVQVISSHIFREGNVCVDRLANLGHS 267
V SH+FRE N D+LA+LGHS
Sbjct: 409 ---LVEFSHVFRESNRAADKLAHLGHS 480
>TC222348
Length = 995
Score = 55.8 bits (133), Expect(2) = 4e-13
Identities = 34/96 (35%), Positives = 49/96 (50%), Gaps = 3/96 (3%)
Frame = -2
Query: 139 KPPSAPWVKVNTDGSVLNNSG--ACGGLFRDHLGTFLGAFVGNLGRCSVFDTEVSGFILV 196
K P PW+K NTD + N G CG LF FLG F ++ S F EV I+
Sbjct: 334 KLPDYPWIKCNTDCAARGNPGLAGCGKLFIYSNARFLGNFAYSIAINSAFFAEVMAAIIA 155
Query: 197 MEHAALHGWYNIWLESDASSALMVFK-NPSLVPILL 231
+E A + W +WL++D+S L K NP + +++
Sbjct: 154 IEKAVENRWNFLWLKTDSSFQLSSAKYNPVIKGLII 47
Score = 35.8 bits (81), Expect(2) = 4e-13
Identities = 28/99 (28%), Positives = 44/99 (44%)
Frame = -3
Query: 10 ETSDHLFLRCKFVVTLWSWLCSQLHVGLDFSSIKALMSSLPRHCSSQVRDLYVAAVVHMV 69
ET DH FL F + +W+ S L+ D SSI ++ AA+
Sbjct: 663 ETVDHFFLYRPFTRQI*NWISSMLNCCSDLSSIHNVL----------------AAITFSF 532
Query: 70 HSIWWARNNVRFSSAKVSSHAVQVRVHAFIGLSGAVSTG 108
IW RN ++F + H + + V + LSG++S+G
Sbjct: 531 FFIWKRRNLIKF----*NQHMIYISVS--VQLSGSISSG 433
>AW277722 similar to SP|O65719|HS73_ Heat shock cognate 70 kDa protein 3
(Hsc70.3). [Mouse-ear cress] {Arabidopsis thaliana},
partial (8%)
Length = 432
Score = 53.9 bits (128), Expect = 9e-08
Identities = 29/61 (47%), Positives = 33/61 (53%), Gaps = 6/61 (9%)
Frame = +1
Query: 138 WKPPSAPWVKVNTDGSVLNN------SGACGGLFRDHLGTFLGAFVGNLGRCSVFDTEVS 191
W+ P WV +NTDGSV N ACGGL RD G +LG F NLG SV E+
Sbjct: 247 WRCPPNGWVCLNTDGSVFENHRNGCSGSACGGLVRDSSGCYLGGFTVNLGNTSVTLAELW 426
Query: 192 G 192
G
Sbjct: 427 G 429
>TC203464 similar to GB|AAF35920.1|7025820|AC005892 L505.8 {Leishmania
major;} , partial (8%)
Length = 1386
Score = 50.8 bits (120), Expect = 7e-07
Identities = 61/235 (25%), Positives = 104/235 (43%), Gaps = 20/235 (8%)
Frame = +2
Query: 74 WARNNVRFSSAKVSSHAVQVRVHAFIGLSGAVSTGKCIAADAAILDAFRIPP-----HRR 128
W+ ++ +SA ++ AV ++A + S + S+ + +A ++ H R
Sbjct: 284 WSSRFLKVASAAAATAAVAGGLYAILSSSTSASSEMEVPFGSARRESKERDDGGSGSHPR 463
Query: 129 SMREIVSVCWKPPSAPWVKVNTDGS---VLNNSGACGGLFRDHLGTFLGAFVGNLG-RCS 184
VS WK P WVK+N DGS ++S CGG+ RD +L F L +
Sbjct: 464 EGATTVSR-WKKPEIGWVKLNVDGSRDPYKSSSAGCGGVLRDASAKWLRGFAKKLNPTYA 640
Query: 185 VFDTEVSGFILVMEHAALHGWYNIWLESDASSALMVFKN------PSL-VPILLRNRWHN 237
V TE+ + ++ A+ + +ESD+ S + + +N P V L+R +
Sbjct: 641 VHQTELEAILTGLKVASEMNVKKLIVESDSDSVVSMVENGVKPNHPDYGVVELIRTK--- 811
Query: 238 ARTLNVQVISSHIFREGNVCVDRLAN----LGHSVVGEVWLSTLPSDFHQVFYED 288
R + +V + + N DRLAN L V E +L P++ +Q+ ED
Sbjct: 812 RRRFDWEVRFVSVSNKANRVADRLANDARKLACDDVCEEYLHP-PANCNQLLLED 973
>BU547962
Length = 591
Score = 50.4 bits (119), Expect = 9e-07
Identities = 29/108 (26%), Positives = 55/108 (50%), Gaps = 4/108 (3%)
Frame = -2
Query: 163 GLFRDHLGTFLGAFVGNLGRCSVFDTEVSGFILVMEHAALHGWYNIWLESDASSAL-MVF 221
G+ R+H FL + ++G+ + E++ +E +GW +IWLE DA + + ++
Sbjct: 590 GVVRNHNAEFLXXYAESIGQANSTIAELTALRKGLELVLENGWNDIWLEGDAKTLVEIIV 411
Query: 222 KNPSLVPILLRNRWHNARTLNVQ---VISSHIFREGNVCVDRLANLGH 266
K + ++ ++ T+ + SHI+REGN D+ A +GH
Sbjct: 410 KRRKVRCTEVQRHINHINTILPEFNNFFVSHIYREGNRAADKFAQMGH 267
>TC208902
Length = 838
Score = 47.0 bits (110), Expect = 1e-05
Identities = 37/131 (28%), Positives = 58/131 (44%), Gaps = 7/131 (5%)
Frame = +1
Query: 145 WVKVNT--DGSVLNNSGACGGLFRDHLGTFLGAFVGNLGRCSVFDTEVSGFILVMEHAAL 202
W ++N+ DG+V ACG + R+ G + F NLG CS+ E+ + ++ A
Sbjct: 223 WGRINSNCDGAVSGGVAACGRVLRNQAGAVV-VFAHNLGMCSISQAEIWAITMGVKLAWD 399
Query: 203 HGWYNIWLESDASSALMVFKNPSLVPILLRNRWHNARTLNVQVISS-----HIFREGNVC 257
G+ N+++ESD A+ + V N + ++ V HI RE N
Sbjct: 400 KGFTNLYVESDFKYAIEMMDG---VCEFSNNCFQLVKSAKDSV*GETYSWCHILREANKT 570
Query: 258 VDRLANLGHSV 268
D LA G V
Sbjct: 571 ADALAKFGVDV 603
>TC213599 homologue to UP|EXBB_PASHA (P72202) Biopolymer transport exbB
protein, partial (8%)
Length = 825
Score = 35.8 bits (81), Expect(2) = 1e-04
Identities = 23/67 (34%), Positives = 33/67 (48%)
Frame = +3
Query: 156 NNSGACGGLFRDHLGTFLGAFVGNLGRCSVFDTEVSGFILVMEHAALHGWYNIWLESDAS 215
N+ ACGGL RD G + +FV LG S F E+ G L + A G N+ + D+
Sbjct: 342 NDMAACGGLLRDCHGRWAASFV*KLGNTSAFVAEL*GAYLGLSLAWDKGDKNLEVNIDSL 521
Query: 216 SALMVFK 222
++ K
Sbjct: 522 VVVVSIK 542
Score = 26.9 bits (58), Expect(2) = 1e-04
Identities = 15/37 (40%), Positives = 18/37 (48%), Gaps = 8/37 (21%)
Frame = +2
Query: 249 HIFREGNVCVDRLANLGHSVVGE--------VWLSTL 277
HI+RE N C D LAN ++ E WLS L
Sbjct: 629 HIYREANGCADALANYACAMEEEYVILEQ*PAWLSQL 739
>TC220201 similar to UP|Q9ERI5 (Q9ERI5) Apoptotic cell clearance receptor
PtdSerR (Phosphatidylserine receptor), partial (5%)
Length = 1185
Score = 42.7 bits (99), Expect = 2e-04
Identities = 19/42 (45%), Positives = 25/42 (59%), Gaps = 3/42 (7%)
Frame = +1
Query: 138 WKPPSAPWVKVNTDGSVLNN---SGACGGLFRDHLGTFLGAF 176
WK P + WVK+N DGS ++ S CGG+ RD GT+ F
Sbjct: 337 WKKPESGWVKLNVDGSRIHEEPASAGCGGVIRDEWGTWCVGF 462
>BQ253293
Length = 420
Score = 42.4 bits (98), Expect = 3e-04
Identities = 19/52 (36%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Frame = +2
Query: 123 IPPHRRSMREIVSVCWKPPSAPWVKVNTDGSV-LNNSGACGGLFRDHLGTFL 173
+PPH + S+CW+PP +K+N + +V N +CGG+ RD G L
Sbjct: 89 LPPHS*DLAVGKSICWEPPPTVQLKLNCEAAVNRNGMASCGGVMRDPYGKVL 244
>CD393541
Length = 557
Score = 41.2 bits (95), Expect = 6e-04
Identities = 29/94 (30%), Positives = 43/94 (44%), Gaps = 4/94 (4%)
Frame = -3
Query: 2 CSFCLEQVETSDHLFLRCKFVVTLW----SWLCSQLHVGLDFSSIKALMSSLPRHCSSQV 57
C C +VE + HLF C+ V LW SW+ SQ HV + L +L S+
Sbjct: 549 CPLCRNEVEDAGHLFFHCRKVTPLWWESMSWV-SQFHVFPKDPKMHFLQHALTTTSGSRA 373
Query: 58 RDLYVAAVVHMVHSIWWARNNVRFSSAKVSSHAV 91
R + + + +IW RN + FS+ S+ V
Sbjct: 372 R-RWKWWWIAVTWTIWKQRNKIIFSNEDFDSNKV 274
>CD416211
Length = 551
Score = 40.4 bits (93), Expect = 0.001
Identities = 29/88 (32%), Positives = 46/88 (51%), Gaps = 2/88 (2%)
Frame = -3
Query: 140 PPSAPWVKVNTDGSVLNNSG--ACGGLFRDHLGTFLGAFVGNLGRCSVFDTEVSGFILVM 197
PP +K+NTDGS N G GG+FR+ G F LG+ V+D G V+
Sbjct: 474 PPDPNTIKINTDGSSFGNPGISGYGGVFRNEHGHVASWF---LGQQWVYD*HKCG---VV 313
Query: 198 EHAALHGWYNIWLESDASSALMVFKNPS 225
++ G ++ ESD++ +L++ +PS
Sbjct: 312 SNSKQKG*QSVICESDSALSLLMVYSPS 229
>TC233956
Length = 757
Score = 37.7 bits (86), Expect = 0.006
Identities = 27/91 (29%), Positives = 42/91 (45%), Gaps = 5/91 (5%)
Frame = +2
Query: 1 MCSFCLEQVETSDHLFLRCKFVVTLW----SWLC-SQLHVGLDFSSIKALMSSLPRHCSS 55
+C FC Q ET+ HLF C ++ LW SW+ ++ + SS S+
Sbjct: 284 LCPFCHSQSETASHLFFTCGKIMPLWWEFLSWVKEDKVFHCRPMDNFLQHYSSAASKVSN 463
Query: 56 QVRDLYVAAVVHMVHSIWWARNNVRFSSAKV 86
R ++ AV +SIW RN++ F + V
Sbjct: 464 TRRTMWWIAV---TNSIWRLRNDIIFQNQTV 547
>CD396188 weakly similar to GP|6175788|gb|A ORF144 {Xestia c-nigrum
granulovirus}, partial (5%)
Length = 701
Score = 36.6 bits (83), Expect = 0.014
Identities = 25/89 (28%), Positives = 45/89 (50%), Gaps = 6/89 (6%)
Frame = -1
Query: 1 MCSFCLEQVETSDHLFLRCKFVVTLW----SWLCSQ--LHVGLDFSSIKALMSSLPRHCS 54
+C FC QVE++ HLF C V+ LW +W+ LH + ++ + R+ +
Sbjct: 512 LCPFCQSQVESASHLFFTCHKVMPLWWEFNTWVREDRVLHSKPMDNFLQHCSLAGSRNSN 333
Query: 55 SQVRDLYVAAVVHMVHSIWWARNNVRFSS 83
+ + ++AA SIW RN++ F++
Sbjct: 332 RRRKIWWIAA----TRSIWNLRNDMIFNN 258
>BI702137
Length = 427
Score = 35.4 bits (80), Expect = 0.031
Identities = 15/33 (45%), Positives = 20/33 (60%), Gaps = 4/33 (12%)
Frame = +2
Query: 1 MCSFCLEQVETSDHLFLRCKFVVTLW----SWL 29
+C FC QVE++ HLF C V+ LW SW+
Sbjct: 182 LCPFCQSQVESASHLFFTCYKVMPLWWEFNSWV 280
>TC222557
Length = 1002
Score = 35.4 bits (80), Expect = 0.031
Identities = 29/89 (32%), Positives = 42/89 (46%), Gaps = 8/89 (8%)
Frame = +1
Query: 1 MCSFCLEQVETSDHLFLRCKFVVTLWSW--LCSQLHVGLDFSSI------KALMSSLPRH 52
MC FC Q E + HLF C ++ LW W L VG+ FS I + + S +H
Sbjct: 553 MCPFCNRQEEEASHLFFNCPRILPLW-WESLAWTKTVGV-FSPIPRQNYMQHTLISKGKH 726
Query: 53 CSSQVRDLYVAAVVHMVHSIWWARNNVRF 81
++ + +VA + SIW RN + F
Sbjct: 727 LQARWKCWWVA----LTWSIWRHRNRIVF 801
>AW832520
Length = 423
Score = 35.4 bits (80), Expect = 0.031
Identities = 23/64 (35%), Positives = 31/64 (47%), Gaps = 6/64 (9%)
Frame = -3
Query: 128 RSMREIVS----VCWKPPSAPWVKVNTDGSVLNNS--GACGGLFRDHLGTFLGAFVGNLG 181
R M EI S + W PP K+N DG+V NS + GG+ RD G F+ F
Sbjct: 292 RLMEEITSYPNGIRWSPPPEGLFKLNYDGAVNCNSLKVSAGGVVRDSHGNFVIGFTTTPD 113
Query: 182 RCSV 185
C++
Sbjct: 112 SCTI 101
>BI973267
Length = 406
Score = 35.0 bits (79), Expect = 0.041
Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 4/33 (12%)
Frame = +1
Query: 1 MCSFCLEQVETSDHLFLRCKFVVTLW----SWL 29
+C FC Q ET+ HLF +C ++ +W SW+
Sbjct: 286 LCPFCHNQPETASHLFFKCDKIMPMWWEFFSWI 384
>TC234500
Length = 866
Score = 35.0 bits (79), Expect = 0.041
Identities = 20/60 (33%), Positives = 30/60 (49%), Gaps = 2/60 (3%)
Frame = +1
Query: 127 RRSMREIVSVCWKPPSAPWVKVNTDGS--VLNNSGACGGLFRDHLGTFLGAFVGNLGRCS 184
+R +++SV P W+K N DGS L + + +FRD G++ F NLG S
Sbjct: 85 KRGHYQMISVE*TFPEVGWIKANVDGSYKALQHLTSSSAVFRDSTGSWCFGFALNLGNLS 264
>BU579105 similar to GP|12328468|dbj P0416D03.16 {Oryza sativa (japonica
cultivar-group)}, partial (4%)
Length = 425
Score = 34.7 bits (78), Expect = 0.053
Identities = 15/32 (46%), Positives = 19/32 (58%), Gaps = 3/32 (9%)
Frame = +3
Query: 138 WKPPSAPWVKVNTDGS---VLNNSGACGGLFR 166
WK P WVK+N DGS ++S CGG+ R
Sbjct: 330 WKKPEIGWVKLNVDGSRDPYKSSSAGCGGVLR 425
>BQ629929
Length = 437
Score = 34.7 bits (78), Expect = 0.053
Identities = 24/89 (26%), Positives = 43/89 (47%), Gaps = 6/89 (6%)
Frame = +3
Query: 1 MCSFCLEQVETSDHLFLRCKFVVTLW----SWLCSQ--LHVGLDFSSIKALMSSLPRHCS 54
+C FC QVE++ HLF K V+ +W SW+ +H + ++ + R +
Sbjct: 132 LCPFCQSQVESASHLFFTYKKVMPMW*EFNSWVKEDTVMHCRPGDNFLQHFSLAGSRDSN 311
Query: 55 SQVRDLYVAAVVHMVHSIWWARNNVRFSS 83
+ + ++AA SIW RN++ F +
Sbjct: 312 RKWKIWWIAATT----SIWNLRNDMIFKN 386
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.326 0.137 0.449
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,253,938
Number of Sequences: 63676
Number of extensions: 277838
Number of successful extensions: 1997
Number of sequences better than 10.0: 87
Number of HSP's better than 10.0 without gapping: 1968
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1989
length of query: 298
length of database: 12,639,632
effective HSP length: 97
effective length of query: 201
effective length of database: 6,463,060
effective search space: 1299075060
effective search space used: 1299075060
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146708.4