BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146708.3 - phase: 0
(282 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BE191551 155 1e-38
BG839197 149 2e-36
AW234180 similar to GP|7108597|gb| K+ transporter HAK5 {Arabidop... 129 1e-30
CA800288 122 2e-28
AW277755 107 8e-24
BU765793 94 7e-20
BU764111 similar to GP|9955527|emb potassium transport protein-l... 80 8e-16
TC224409 similar to UP|POT6_ARATH (Q8W4I4) Potassium transporter... 74 6e-14
TC211717 similar to UP|POT1_ARATH (O22397) Potassium transporter... 68 4e-12
AW756492 similar to GP|9955527|emb| potassium transport protein-... 52 3e-07
BE331537 30 1.6
TC231884 similar to UP|O81445 (O81445) Calcineurin B-like protei... 28 3.6
TC207861 similar to UP|MPK7_ARATH (Q39027) Mitogen-activated pro... 28 6.1
TC231983 27 8.0
BM528411 27 8.0
TC225844 similar to UP|Q9LW29 (Q9LW29) Transport inhibitor respo... 27 8.0
AW508044 27 8.0
>BE191551
Length = 475
Score = 155 bits (393), Expect = 1e-38
Identities = 75/152 (49%), Positives = 104/152 (68%), Gaps = 3/152 (1%)
Frame = -3
Query: 127 LLLFMTILGTTMVIGDGVFTPPMSVISAVNGIS---SKLGQDYVVSITIAILVILFCAQR 183
++L + ++GT MVIGDG+ TP +SV+SAV GI + L + VV + + ILV LF Q
Sbjct: 467 IILMLALVGTCMVIGDGILTPAISVLSAVGGIKVNHADLSNEVVVLVAVVILVGLFSMQH 288
Query: 184 FGTSKVGFSFAPILTIWFILIGATGIYNVFKYDVRVLLAINPKYIVDYFQRNGKNAWMSL 243
+GT KVG+ FAPI+ +WF+LIG GI+N+ Y VL A +P YI Y QR GK+ W+SL
Sbjct: 287 YGTDKVGWLFAPIVLLWFLLIGGIGIFNICXYGSSVLKAFSPLYIYRYLQREGKDGWLSL 108
Query: 244 GGVFLCISGCEAMFADLGHFNVRAIQVSIIKL 275
GG+ L I+G EA+FADL HF V ++Q++ L
Sbjct: 107 GGILLSITGTEALFADLAHFPVSSVQIAFTML 12
>BG839197
Length = 816
Score = 149 bits (375), Expect = 2e-36
Identities = 71/120 (59%), Positives = 87/120 (72%)
Frame = +2
Query: 152 ISAVNGISSKLGQDYVVSITIAILVILFCAQRFGTSKVGFSFAPILTIWFILIGATGIYN 211
IS + + G VV I+I +LV LF QRFGTSKVGF FAPIL +WF +GA GIYN
Sbjct: 2 ISGLQDQIDEFGTGEVVGISIVVLVALFSIQRFGTSKVGFMFAPILALWFFSLGAIGIYN 181
Query: 212 VFKYDVRVLLAINPKYIVDYFQRNGKNAWMSLGGVFLCISGCEAMFADLGHFNVRAIQVS 271
+ KYD+ VL A NP YI +F+ NGK+AW +LGG LCI+G EAMFADLGHF+V AIQ++
Sbjct: 182 ILKYDITVLRAFNPAYIYYFFKNNGKDAWSALGGCVLCITGAEAMFADLGHFSVPAIQIA 361
>AW234180 similar to GP|7108597|gb| K+ transporter HAK5 {Arabidopsis
thaliana}, partial (16%)
Length = 401
Score = 129 bits (325), Expect = 1e-30
Identities = 56/78 (71%), Positives = 67/78 (85%)
Frame = +2
Query: 194 APILTIWFILIGATGIYNVFKYDVRVLLAINPKYIVDYFQRNGKNAWMSLGGVFLCISGC 253
API+ +WF+ IG G+YN+FKYD+ VL A NPKYI DYF+RNGK W+SLGGVFLCI+G
Sbjct: 8 APIILVWFLFIGGIGLYNLFKYDIGVLRAFNPKYIYDYFKRNGKEGWISLGGVFLCITGS 187
Query: 254 EAMFADLGHFNVRAIQVS 271
EAMFADLGHFNVR+IQ+S
Sbjct: 188 EAMFADLGHFNVRSIQIS 241
>CA800288
Length = 435
Score = 122 bits (306), Expect = 2e-28
Identities = 69/145 (47%), Positives = 89/145 (60%), Gaps = 6/145 (4%)
Frame = +1
Query: 73 GTFALYSLLCRHANVSLIPNQQPEDMELSNYKLETPS---SNQQLKKKLENSHFARVLLL 129
GTFALYSLLCRHA +L+PNQQ D ELS+YK S ++ LK+ LE R LL
Sbjct: 1 GTFALYSLLCRHAKFNLLPNQQAADEELSSYKYGPSSQAIASSPLKRFLEKHKRLRTALL 180
Query: 130 FMTILGTTMVIGDGVFTPPMSVISAVNGI---SSKLGQDYVVSITIAILVILFCAQRFGT 186
+ + G MVIGDGV TP +SV+++V+G+ KL +V + ILV LF Q GT
Sbjct: 181 VVVLFGACMVIGDGVLTPAISVLASVSGLKVTEKKLTDGELVLLACVILVGLFALQHCGT 360
Query: 187 SKVGFSFAPILTIWFILIGATGIYN 211
KV FAPI+ IW + I + G+YN
Sbjct: 361 HKVAVMFAPIVIIWLVSIFSIGVYN 435
>AW277755
Length = 437
Score = 107 bits (266), Expect = 8e-24
Identities = 58/116 (50%), Positives = 74/116 (63%), Gaps = 3/116 (2%)
Frame = +1
Query: 2 ATLALAFQSLGVVYGDIGTSPLYVLASTFP---KGIDHTDDILGVLSLIYYTILALPLLK 58
A L LA+QSLG ++GD+ SPLYV S F K + H D I G SLI++T+ + LLK
Sbjct: 67 ALLFLAYQSLGFMFGDLTLSPLYVYQSIFSGRLKNVQHEDAIFGAFSLIFWTLSIISLLK 246
Query: 59 YVFIVLKANDNGNGGTFALYSLLCRHANVSLIPNQQPEDMELSNYKLETPSSNQQL 114
Y I+L A+DNG GG ALYS LCR+A L+PN Q D ELS Y + SSN+ +
Sbjct: 247 YAIIMLSADDNGEGGIVALYSHLCRNAKFCLLPNHQASDEELSTYH-KPGSSNRNI 411
>BU765793
Length = 424
Score = 94.0 bits (232), Expect = 7e-20
Identities = 47/78 (60%), Positives = 57/78 (72%), Gaps = 3/78 (3%)
Frame = +1
Query: 4 LALAFQSLGVVYGDIGTSPLYVLASTFPKGIDHTDD---ILGVLSLIYYTILALPLLKYV 60
L LA+QS GVVYGD+ TSPLYV STF + + D I G SLI++T+ +PLLKYV
Sbjct: 190 LLLAYQSFGVVYGDLSTSPLYVFTSTFKGKLQNHHDEETIFGTFSLIFWTLTLIPLLKYV 369
Query: 61 FIVLKANDNGNGGTFALY 78
FI+L A+DNG GGTFALY
Sbjct: 370 FILLSADDNGEGGTFALY 423
>BU764111 similar to GP|9955527|emb potassium transport protein-like
{Arabidopsis thaliana}, partial (16%)
Length = 445
Score = 80.5 bits (197), Expect = 8e-16
Identities = 40/74 (54%), Positives = 52/74 (70%)
Frame = +3
Query: 208 GIYNVFKYDVRVLLAINPKYIVDYFQRNGKNAWMSLGGVFLCISGCEAMFADLGHFNVRA 267
GIYN+ KYD VL A NP +I +F+RN NAW SLGG L +G EAMFADL +F+VR+
Sbjct: 12 GIYNLVKYDNSVLRAFNPIHIYYFFKRNSTNAWYSLGGCLLSATGSEAMFADLCYFSVRS 191
Query: 268 IQVSIIKLELTFVL 281
+Q+S + L L +L
Sbjct: 192 VQLSFVFLVLPCLL 233
>TC224409 similar to UP|POT6_ARATH (Q8W4I4) Potassium transporter 6 (AtPOT6)
(AtHAK6), partial (22%)
Length = 529
Score = 74.3 bits (181), Expect = 6e-14
Identities = 30/66 (45%), Positives = 48/66 (72%)
Frame = +1
Query: 206 ATGIYNVFKYDVRVLLAINPKYIVDYFQRNGKNAWMSLGGVFLCISGCEAMFADLGHFNV 265
A G+YN+F ++ V A++P Y+ + ++ K WMSLGG+ LCI+G EAM+ADLGHF+
Sbjct: 1 AIGVYNIFHWNPHVYEALSPYYMFKFLKKTQKGGWMSLGGILLCITGSEAMYADLGHFSQ 180
Query: 266 RAIQVS 271
+I+++
Sbjct: 181 LSIKIA 198
>TC211717 similar to UP|POT1_ARATH (O22397) Potassium transporter 1 (AtPOT1)
(AtKUP1) (AtKT1), partial (11%)
Length = 570
Score = 68.2 bits (165), Expect = 4e-12
Identities = 33/70 (47%), Positives = 48/70 (68%), Gaps = 3/70 (4%)
Frame = +3
Query: 4 LALAFQSLGVVYGDIGTSPLYVLASTFPKGI---DHTDDILGVLSLIYYTILALPLLKYV 60
L LA+QSLGVVYGD+ + P+YV ++F + + ++I GVLS I++T + L KYV
Sbjct: 189 LTLAYQSLGVVYGDLSSVPIYVYKTSFSGKLSLKEDNEEIFGVLSFIFWTFTIIGLFKYV 368
Query: 61 FIVLKANDNG 70
FIV+ +DNG
Sbjct: 369 FIVMSTDDNG 398
>AW756492 similar to GP|9955527|emb| potassium transport protein-like
{Arabidopsis thaliana}, partial (9%)
Length = 439
Score = 52.0 bits (123), Expect = 3e-07
Identities = 26/45 (57%), Positives = 33/45 (72%), Gaps = 1/45 (2%)
Frame = +1
Query: 4 LALAFQSLGVVYGDIGTSPLYVLASTFPKG-IDHTDDILGVLSLI 47
+ LAFQ+LGVV+GD+GTSPLY + F K I +DILG LSL+
Sbjct: 304 ILLAFQTLGVVFGDVGTSPLYTFSVMFRKAPIKGNEDILGALSLV 438
>BE331537
Length = 628
Score = 29.6 bits (65), Expect = 1.6
Identities = 25/89 (28%), Positives = 43/89 (48%), Gaps = 9/89 (10%)
Frame = +2
Query: 124 ARVLLLFMTILGTTMVIGDGVFTPPMS--------VISAVNGISSKLGQDYVVSIT-IAI 174
A++ L + I+ M IG+GV P M+ V ++ Y+ S+T +A
Sbjct: 152 AKLGLPCLLIMRAFMGIGEGVAMPAMNNILSKWIPVSERSRSLALVYSDMYLGSVTGLAF 331
Query: 175 LVILFCAQRFGTSKVGFSFAPILTIWFIL 203
IL Q+FG V +S+ + +IWF++
Sbjct: 332 SPILI--QKFGWPSVFYSYGSLGSIWFVV 412
>TC231884 similar to UP|O81445 (O81445) Calcineurin B-like protein 1, partial
(76%)
Length = 670
Score = 28.5 bits (62), Expect = 3.6
Identities = 18/52 (34%), Positives = 29/52 (55%)
Frame = -3
Query: 150 SVISAVNGISSKLGQDYVVSITIAILVILFCAQRFGTSKVGFSFAPILTIWF 201
+VIS+ ++ KLG+D V+S+ V++F + F +KV S I WF
Sbjct: 491 AVISSTLELNIKLGKDVVMSLR*G-RVMIFNSDGFFLTKVSHSSVSIFPSWF 339
>TC207861 similar to UP|MPK7_ARATH (Q39027) Mitogen-activated protein kinase
homolog 7 (MAP kinase 7) (AtMPK7) , partial (67%)
Length = 911
Score = 27.7 bits (60), Expect = 6.1
Identities = 16/50 (32%), Positives = 24/50 (48%), Gaps = 4/50 (8%)
Frame = +2
Query: 152 ISAVNGISSKLGQDYVVSITIAILVILFCAQRFGTS----KVGFSFAPIL 197
++ NG+ + +YVV+ +L C +GTS VG FA IL
Sbjct: 167 LARTNGVDGQFMTEYVVTRWYRAPELLLCCDNYGTSIDVWSVGCIFAEIL 316
>TC231983
Length = 526
Score = 27.3 bits (59), Expect = 8.0
Identities = 16/76 (21%), Positives = 38/76 (49%)
Frame = +1
Query: 161 KLGQDYVVSITIAILVILFCAQRFGTSKVGFSFAPILTIWFILIGATGIYNVFKYDVRVL 220
+LG + +++A V + + S +GF FA LT W +++ ++ + ++ V
Sbjct: 277 QLGVIGALFLSVASSVSIVICNKALMSNLGFPFATTLTSWHLMVTFCTLHAAQRLNLFVS 456
Query: 221 LAINPKYIVDYFQRNG 236
+++ K ++ + NG
Sbjct: 457 KSVDSKTVMLFGILNG 504
>BM528411
Length = 368
Score = 27.3 bits (59), Expect = 8.0
Identities = 25/99 (25%), Positives = 46/99 (46%), Gaps = 8/99 (8%)
Frame = +1
Query: 46 LIYYTILALPLLKYVFIVLKANDNGNGGTFALYS-------LLCR-HANVSLIPNQQPED 97
LI I+ +P +L A+ G G +Y L+C H+NV ++P+Q E
Sbjct: 85 LIMTVIIGIP-------ILGASLMGYGSASMIYGYVLIFDFLICLGHSNVEIVPHQLFEK 243
Query: 98 MELSNYKLETPSSNQQLKKKLENSHFARVLLLFMTILGT 136
+ Y + TP+ + L ++++F + LF ++ T
Sbjct: 244 LPFLRYVIYTPTYH-HLHHSDKDTNFCLFMPLFDSLGNT 357
>TC225844 similar to UP|Q9LW29 (Q9LW29) Transport inhibitor response-like
protein (At3g26830), partial (61%)
Length = 1347
Score = 27.3 bits (59), Expect = 8.0
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = -3
Query: 17 DIGTSPLYVLASTFPKGID 35
DI TSP V A+ FPKG D
Sbjct: 499 DIATSPFSVTAAGFPKGTD 443
>AW508044
Length = 437
Score = 27.3 bits (59), Expect = 8.0
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = -3
Query: 240 WMSLGGVFLCISGC 253
W+SL G F+C SGC
Sbjct: 192 WLSLAGGFVCFSGC 151
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.326 0.143 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,231,118
Number of Sequences: 63676
Number of extensions: 168678
Number of successful extensions: 1024
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 1016
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1018
length of query: 282
length of database: 12,639,632
effective HSP length: 96
effective length of query: 186
effective length of database: 6,526,736
effective search space: 1213972896
effective search space used: 1213972896
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146708.3