BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146651.18 + phase: 0 /pseudo
(559 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC208963 142 3e-34
BM525744 117 2e-26
TC210791 58 2e-17
TC234242 71 1e-12
TC211617 69 7e-12
BE057981 63 4e-10
BM892478 60 3e-09
AW781991 52 5e-07
BI786825 50 3e-06
AW569215 weakly similar to GP|4914326|gb| F14N23.12 {Arabidopsis... 47 2e-05
TC224081 46 4e-05
BG882562 46 5e-05
AW761314 45 1e-04
AW200845 40 0.002
TC218095 39 0.005
AW507853 37 0.023
TC218096 37 0.030
BI701986 36 0.040
BM732165 homologue to SP|Q9TV66|HCN4 Potassium/sodium hyperpolar... 34 0.20
TC221563 similar to UP|O23702 (O23702) Dehydrogenase, partial (21%) 33 0.26
>TC208963
Length = 1163
Score = 142 bits (359), Expect = 3e-34
Identities = 111/370 (30%), Positives = 176/370 (47%), Gaps = 54/370 (14%)
Frame = +1
Query: 147 NMPKVIVTDRDMSLMKAVENIFPKSYTMNCYFHVQANVKQRCVLDFKYPLGKRDGKEVKP 206
+MP+VIVT D++LM AV+ +FP S + C FH+ NVK +C K + ++ +E+
Sbjct: 16 DMPQVIVTVGDIALMSAVQVVFPSSSNLLCRFHINQNVKAKC----KSIVHLKEKQEL-- 177
Query: 207 RDVVKKIMRA*KAMVELPTQELYANASVEFQNSCSDFSLFKNYAMTT------------- 253
+M A +V P + Y F+N C DF + +Y T
Sbjct: 178 ------MMDAWDVVVNSPNEGEYMQRLAFFENVCLDFPILGDYVKNTWLIPHKEKFVTAW 339
Query: 254 LNEV------------------------------KEKIVRAWTDHVLHLGCRTSNMVESA 283
N+V KEK V AWT+ V+HLG +N VE+
Sbjct: 340 TNQVMHLGNTATNRFAQTLYFE*FIRKNT*LIPHKEKFVTAWTNRVMHLGNTATNRVEAT 519
Query: 284 HALLKKYLDNSVGDLGTFWEKIHDMLLLQFTAIQTTFGQSVTVLEHRFKDVILYSDLGGH 343
H LK L +S D+ ++W+ + +M+ LQ T I+ +F +S V+EHR + Y L G
Sbjct: 520 HWRLKTLLQDSKEDMCSYWDAMKNMITLQHTEIEASFEKSKNVVEHR-HNTPFYVKLVGF 696
Query: 344 VSRYALDNSALEETRCRETLCIENNI---VQRTSYGLPCA*LLEE-----KPILLDELLH 395
VSR AL + E R + T I+++I + RT++GLPCA L PI L+ +
Sbjct: 697 VSRSALSHITEEYDRVK-TAGIDSSICGCIVRTTHGLPCACELARYSTMCHPIPLEAIHA 873
Query: 396 H*HRLRM---GEESNEVGFCVKVELKAIVEHIKKLLFQMKLEVKEGLRQLTFSETTMMSP 452
H +L+ G N ++ E+ A+ + ++L + K+ + L ++ F +T +
Sbjct: 874 HWRKLKFSDHGTNDNGSELSLQPEVDALYKRFQELDYAGKIILMAKLHEMAFPDTALKCL 1053
Query: 453 PPRKVPTKGA 462
PP +V TKGA
Sbjct: 1054PPEEVGTKGA 1083
>BM525744
Length = 411
Score = 117 bits (292), Expect = 2e-26
Identities = 54/125 (43%), Positives = 81/125 (64%)
Frame = +3
Query: 3 PKNILIHLKDKRPHCMTNVKQVYNERQQIRKANRGDKKSLQYLISKLEEHNYTYYSRTQS 62
P+NIL+ LKD T ++ +YN RQ R + +G + +Q+L+ LE Y +SR
Sbjct: 39 PRNILLTLKDHNND--TTIRHIYNARQAYRSSQKGPRTEMQHLLKLLEHDQYVCWSRKVD 212
Query: 63 ESTTIEDIFWAHPTFVKLFNNFPTVLVMDSTYKTNMYKMLMFEVVGVTSTDLTYSVGFGF 122
+S I DIFWAHP +KL +F TVL +D+TYK N Y++ + E+VGVTST+LT+SV F +
Sbjct: 213 DSDAIRDIFWAHPDAIKLLGSFHTVLFLDNTYKVNRYQLPLLEIVGVTSTELTFSVAFAY 392
Query: 123 VTHEK 127
+ ++
Sbjct: 393 MESDE 407
>TC210791
Length = 456
Score = 58.2 bits (139), Expect(2) = 2e-17
Identities = 34/96 (35%), Positives = 49/96 (50%), Gaps = 1/96 (1%)
Frame = +2
Query: 204 VKPRDVVKKIMRA*KAMVELPTQELYANASVEFQNSCSDFSLFKNYAMTT-LNEVKEKIV 262
V P++ +M A +++ P + Y + CSDF F +Y T L KEK V
Sbjct: 161 VHPKERYDLVMDAWDSVMNSPNEGEYMQRLTLLEKVCSDFPTFGDYVKNTWLIPHKEKFV 340
Query: 263 RAWTDHVLHLGCRTSNMVESAHALLKKYLDNSVGDL 298
AW D V+HLG T + E+AH L+ L +S GD+
Sbjct: 341 MAWVDRVMHLGNTTIDRFETAHWRLENLLQDSGGDM 448
Score = 49.3 bits (116), Expect(2) = 2e-17
Identities = 23/53 (43%), Positives = 33/53 (61%)
Frame = +1
Query: 139 RKLLTSKMNMPKVIVTDRDMSLMKAVENIFPKSYTMNCYFHVQANVKQRCVLD 191
R L+ MP VI+T RD++LM AV+ +FP S + C FH+ NVK + +D
Sbjct: 1 RGLIFKDNEMPPVIITVRDIALMDAVQVVFPSSSNLLCRFHISKNVKAKSQVD 159
>TC234242
Length = 478
Score = 70.9 bits (172), Expect = 1e-12
Identities = 35/96 (36%), Positives = 56/96 (57%), Gaps = 5/96 (5%)
Frame = +3
Query: 40 KSLQYLISKLEEHNYTYYSRTQSESTTIEDIFWAHPTFVKLFNNFPTVLVMDSTYKTNMY 99
+ +Q+L+ LE Y ++ R + E + DIFW HP VKL N V ++DSTY+TN Y
Sbjct: 12 EEMQHLMKFLERDQYIHWHRIKDEDV-VRDIFWCHPDSVKLVNACNLVFLIDSTYRTNRY 188
Query: 100 KM-----LMFEVVGVTSTDLTYSVGFGFVTHEKEEN 130
++ ++ + VG+T T +T+S F +V E+ N
Sbjct: 189 RLPLLDFVVLDFVGMTPTGMTFSASFAYVEGERVNN 296
>TC211617
Length = 534
Score = 68.6 bits (166), Expect = 7e-12
Identities = 32/91 (35%), Positives = 51/91 (55%)
Frame = -1
Query: 3 PKNILIHLKDKRPHCMTNVKQVYNERQQIRKANRGDKKSLQYLISKLEEHNYTYYSRTQS 62
P+NI + LK+ + T +KQ+YN R + RGD +Q+L+ L+ Y ++ R +
Sbjct: 279 PRNIQLTLKEHNTNSCTTIKQIYNARSAYHSSIRGDDTEMQHLMRLLKRDQYIHWHRLKD 100
Query: 63 ESTTIEDIFWAHPTFVKLFNNFPTVLVMDST 93
+ + D+FW HP VKL N V ++DST
Sbjct: 99 QD-VVRDLFWCHPDAVKLCNTCHLVFLIDST 10
>BE057981
Length = 268
Score = 62.8 bits (151), Expect = 4e-10
Identities = 29/81 (35%), Positives = 47/81 (57%)
Frame = +2
Query: 50 EEHNYTYYSRTQSESTTIEDIFWAHPTFVKLFNNFPTVLVMDSTYKTNMYKMLMFEVVGV 109
E Y ++ R + E + D+FW H VKL N V ++++ YKTN Y++L+ + GV
Sbjct: 14 ERDQYIHWHRLKDEFV-VRDLFWCHLDAVKLCNACHLVFLINNAYKTNRYRLLLLDFAGV 190
Query: 110 TSTDLTYSVGFGFVTHEKEEN 130
T +T+SVGF ++ E+ N
Sbjct: 191 TPARMTFSVGFAYLEGERVNN 253
>BM892478
Length = 429
Score = 59.7 bits (143), Expect = 3e-09
Identities = 31/131 (23%), Positives = 68/131 (51%)
Frame = +3
Query: 42 LQYLISKLEEHNYTYYSRTQSESTTIEDIFWAHPTFVKLFNNFPTVLVMDSTYKTNMYKM 101
L+Y S+ E +Y+ + ++ +IFWA ++F VLV+D++Y+ +Y +
Sbjct: 33 LEYFQSRQAEDTGFFYAM-EVDNGNCMNIFWADGRSRYSCSHFGDVLVLDTSYRKTVYLV 209
Query: 102 LMFEVVGVTSTDLTYSVGFGFVTHEKEENFVWVLKILRKLLTSKMNMPKVIVTDRDMSLM 161
VGV +G + E EE+F W+ + + ++ + +P ++ D+D+++
Sbjct: 210 PFATFVGVNHHKQPVLLGCALIADESEESFTWLFQTWLRAMSGR--LPLTVIADQDIAIQ 383
Query: 162 KAVENIFPKSY 172
+A+ +FP ++
Sbjct: 384 RAIAKVFPVTH 416
>AW781991
Length = 419
Score = 52.4 bits (124), Expect = 5e-07
Identities = 29/112 (25%), Positives = 49/112 (42%)
Frame = +3
Query: 25 YNERQQIRKANRGDKKSLQYLISKLEEHNYTYYSRTQSESTTIEDIFWAHPTFVKLFNNF 84
Y ++R + L YL E+ +Y+ E +I ++FWA P + F
Sbjct: 78 YMRNNRLRSLEGDIQLVLDYLRQMHAENPNFFYAVQGDEDQSITNVFWADPKARMNYTFF 257
Query: 85 PTVLVMDSTYKTNMYKMLMFEVVGVTSTDLTYSVGFGFVTHEKEENFVWVLK 136
+ D+TY++N Y++ GV G F+ +E E +FVW+ K
Sbjct: 258 GDTVTFDTTYRSNRYRLPFAFFTGVNHHGQPVLFGCAFLINESEASFVWLFK 413
>BI786825
Length = 421
Score = 50.1 bits (118), Expect = 3e-06
Identities = 34/130 (26%), Positives = 60/130 (46%), Gaps = 6/130 (4%)
Frame = +1
Query: 435 VKEGLRQLTFSETTMMSPPPRKVPTKGAKKKVDIARLLIPKIRIVNRHHHQQHH------ 488
+K LR++ + + M PP KV TKGA KK +NR+
Sbjct: 1 LKSKLREIAYPDQNSMCHPPAKVNTKGAPKKS------------MNRNPRSTKRDPSYWK 144
Query: 489 HIKEKKDVRLGKTSLNPLSPPFRYPKPKTILVMRPIDYMSRYMLPFIEKMVDVIGDGHCG 548
++ V+ +S+ P P+ I+ M +D ++ FI+ +VDV D +CG
Sbjct: 145 YVDAFHSVQNSNSSVRHSVSSSYQPNPRRIMPM--LDQFQSFIQDFIDNIVDVKPDRNCG 318
Query: 549 FRAIAAFMGL 558
+R++A+ +G+
Sbjct: 319 YRSVASLLGM 348
>AW569215 weakly similar to GP|4914326|gb| F14N23.12 {Arabidopsis thaliana},
partial (19%)
Length = 425
Score = 47.0 bits (110), Expect = 2e-05
Identities = 26/93 (27%), Positives = 46/93 (48%)
Frame = +1
Query: 88 LVMDSTYKTNMYKMLMFEVVGVTSTDLTYSVGFGFVTHEKEENFVWVLKILRKLLTSKMN 147
+V D+TY+ Y ML+ +GV + +T + E ++F W LK + K
Sbjct: 10 VVFDTTYRVEAYDMLLGIWLGVDNNGMTCFFSCALLRDENIQSFSWALKAFLGFMKGK-- 183
Query: 148 MPKVIVTDRDMSLMKAVENIFPKSYTMNCYFHV 180
P+ I+TD +M L +A+ P++ C +H+
Sbjct: 184 APQTILTDHNMWLKEAIAVELPQTKHAFCIWHI 282
>TC224081
Length = 512
Score = 46.2 bits (108), Expect = 4e-05
Identities = 36/149 (24%), Positives = 66/149 (44%)
Frame = +1
Query: 408 EVGFCVKVELKAIVEHIKKLLFQMKLEVKEGLRQLTFSETTMMSPPPRKVPTKGAKKKVD 467
E + E + I++ ++L KL +K L ++ + M PPP KV TKGA KK
Sbjct: 82 ETQVTITEEKETILKRFEQLDVCGKLHLKSKL*EIAYPNMNSMCPPPEKVKTKGASKKP- 258
Query: 468 IARLLIPKIRIVNRHHHQQHHHIKEKKDVRLGKTSLNPLSPPFRYPKPKTILVMRPIDYM 527
+ K + + + ++ V+ + + + P + + M +D
Sbjct: 259 -----LTKQQKSTKRYPSYWKYVDVLHSVQNSNSLVKHSASSSEEPIQRWNIPM--LDQF 417
Query: 528 SRYMLPFIEKMVDVIGDGHCGFRAIAAFM 556
+ IE ++ V +G+CG+RAIAA +
Sbjct: 418 HPCIQDSIENIIGVKANGNCGYRAIAALL 504
>BG882562
Length = 416
Score = 45.8 bits (107), Expect = 5e-05
Identities = 26/133 (19%), Positives = 61/133 (45%), Gaps = 1/133 (0%)
Frame = +3
Query: 36 RGDKKSLQYLISKLEEHNYTY-YSRTQSESTTIEDIFWAHPTFVKLFNNFPTVLVMDSTY 94
+GD + + +++ N + Y ++ + ++FW ++ F V+ DST
Sbjct: 24 KGDPELISNYFCRIQLMNPNFFYVMDLNDDGQLRNVFWIDSRSRAAYSYFGDVVAFDSTC 203
Query: 95 KTNMYKMLMFEVVGVTSTDLTYSVGFGFVTHEKEENFVWVLKILRKLLTSKMNMPKVIVT 154
+N Y++ + VGV + +G G + E E ++W+ + +T + P+ I+T
Sbjct: 204 LSNNYEIPLVAFVGVNHHGKSVLLGCGLLADETFETYIWLFRAWLTCMTGR--PPQTIIT 377
Query: 155 DRDMSLMKAVENI 167
+ ++ A+ +
Sbjct: 378 NXCKAMQSAIAEV 416
>AW761314
Length = 331
Score = 44.7 bits (104), Expect = 1e-04
Identities = 21/57 (36%), Positives = 32/57 (55%)
Frame = +1
Query: 3 PKNILIHLKDKRPHCMTNVKQVYNERQQIRKANRGDKKSLQYLISKLEEHNYTYYSR 59
PKNIL+ LK+ + T +KQ+YN R + RG +Q L+ LE+ Y ++ R
Sbjct: 160 PKNILLTLKEHNVNSYTAIKQIYNARSAYCSSIRGSDTKMQQLMKLLEQDQYIHWHR 330
>AW200845
Length = 343
Score = 40.4 bits (93), Expect = 0.002
Identities = 21/60 (35%), Positives = 37/60 (61%), Gaps = 1/60 (1%)
Frame = +1
Query: 220 MVELPTQELYANASVEFQNSCSDFSLFKNYAMTT-LNEVKEKIVRAWTDHVLHLGCRTSN 278
+V+ P++ +A + +FQ + + +F +Y T + KEKI+ AWT+ V+HLG T+N
Sbjct: 91 LVDCPSEH*FAESLQKFQIA*PPWPMFVDYVNDTWIIPHKEKIITAWTNKVMHLGNTTTN 270
>TC218095
Length = 925
Score = 39.3 bits (90), Expect = 0.005
Identities = 15/26 (57%), Positives = 22/26 (83%)
Frame = +1
Query: 533 PFIEKMVDVIGDGHCGFRAIAAFMGL 558
PFIE ++DV GD +CG+RA+AA +G+
Sbjct: 130 PFIEDIIDVKGDSNCGYRAVAAQLGM 207
>AW507853
Length = 440
Score = 37.0 bits (84), Expect = 0.023
Identities = 28/102 (27%), Positives = 49/102 (47%)
Frame = +1
Query: 457 VPTKGAKKKVDIARLLIPKIRIVNRHHHQQHHHIKEKKDVRLGKTSLNPLSPPFRYPKPK 516
V TKGA KK + K + + ++ V+ +S+ + P+P
Sbjct: 1 VNTKGAPKKP------MKKSQRSTKRDPSYWEYVDAFHSVQSSNSSVKCSASCSEPPQPT 162
Query: 517 TILVMRPIDYMSRYMLPFIEKMVDVIGDGHCGFRAIAAFMGL 558
TI+ M +D + ++ FI +VDV D + G+R+IAA +G+
Sbjct: 163 TIISM--LDQFAPFIQGFIRDVVDVKADDNYGYRSIAAXLGM 282
>TC218096
Length = 806
Score = 36.6 bits (83), Expect = 0.030
Identities = 14/26 (53%), Positives = 21/26 (79%)
Frame = +3
Query: 533 PFIEKMVDVIGDGHCGFRAIAAFMGL 558
PFIE ++DV G +CG+RA+AA +G+
Sbjct: 63 PFIEDIIDVKGXSNCGYRAVAAQLGM 140
>BI701986
Length = 422
Score = 36.2 bits (82), Expect = 0.040
Identities = 11/25 (44%), Positives = 19/25 (76%)
Frame = +2
Query: 468 IARLLIPKIRIVNRHHHQQHHHIKE 492
++ LL+P++ I++ HHH HHH +E
Sbjct: 29 LSSLLLPRLMIIHHHHHHHHHHPRE 103
>BM732165 homologue to SP|Q9TV66|HCN4 Potassium/sodium
hyperpolarization-activated cyclic nucleotide-gated
channel 4, partial (2%)
Length = 423
Score = 33.9 bits (76), Expect = 0.20
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +1
Query: 471 LLIPKIRIVNRHHHQQHHH 489
LL+P++ I++ HHH HHH
Sbjct: 4 LLLPRLMIIHHHHHHHHHH 60
>TC221563 similar to UP|O23702 (O23702) Dehydrogenase, partial (21%)
Length = 486
Score = 33.5 bits (75), Expect = 0.26
Identities = 23/81 (28%), Positives = 37/81 (45%), Gaps = 4/81 (4%)
Frame = +3
Query: 453 PPRKVPTKGAKKKVDIARLLIPKIRIVNRHHHQQ----HHHIKEKKDVRLGKTSLNPLSP 508
PPR+ P + R L+P+IRI R H++ ++ + VR + P P
Sbjct: 90 PPRRHPK--------LRRGLLPRIRIPRRRRHRRTCSPQPPLRRQDRVRRRRAPPLPRLP 245
Query: 509 PFRYPKPKTILVMRPIDYMSR 529
P R P P +L P+ ++ R
Sbjct: 246 PPRRPAPPPLLPPHPLPWVRR 308
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.325 0.139 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,559,286
Number of Sequences: 63676
Number of extensions: 336677
Number of successful extensions: 4166
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 3325
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3861
length of query: 559
length of database: 12,639,632
effective HSP length: 102
effective length of query: 457
effective length of database: 6,144,680
effective search space: 2808118760
effective search space used: 2808118760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146651.18