BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146650.9 - phase: 0 /pseudo
(314 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC209527 similar to GB|AAM10021.1|20148261|AY081459 formyltetrah... 382 e-106
TC233275 similar to UP|O23579 (O23579) Formyltransferase purU ho... 152 2e-37
AW317195 106 1e-36
AI441435 91 8e-30
TC232723 similar to UP|O23579 (O23579) Formyltransferase purU ho... 54 1e-07
BG239393 weakly similar to GP|16930491|gb| At1g06110/T21E18_13 {... 29 3.2
BU577659 weakly similar to GP|8809619|dbj| contains similarity t... 28 4.1
TC232261 weakly similar to UP|Q65198 (Q65198) PE199L, partial (7%) 28 5.4
CK606661 28 7.0
BG239755 28 7.0
BU577461 similar to PIR|T11622|T11 extensin class 1 precursor - ... 27 9.2
BG650512 27 9.2
AW317643 similar to GP|12484392|gb cellulase {Fragaria x ananass... 27 9.2
TC210418 similar to UP|Q9FMX6 (Q9FMX6) Gb|AAD21732.1, partial (36%) 27 9.2
CB063867 27 9.2
TC225931 27 9.2
>TC209527 similar to GB|AAM10021.1|20148261|AY081459 formyltetrahydrofolate
deformylase-like {Arabidopsis thaliana;} , partial (81%)
Length = 981
Score = 382 bits (980), Expect = e-106
Identities = 213/316 (67%), Positives = 227/316 (71%), Gaps = 9/316 (2%)
Frame = +3
Query: 1 MGMVVRRVSQVLGLTNSNKIRNFSFKSLDLPPLPSPSLSHGIHVFHCPDAVGIVAKLSEC 60
M M R VLG RN SFKSLD P SL+HGIHVFHCPDAVGIVAKLS+C
Sbjct: 57 MAMARRVPRYVLGSVQ----RNMSFKSLD----PPSSLTHGIHVFHCPDAVGIVAKLSDC 212
Query: 61 IASRGGNILAADVFVPQNKHVFYSRSDFVFDPVKWPRKQMEEDFLKLSQAFNATRSVVRV 120
IASRGGNILAADVFVP+NK VFYSRSDFVFDPVKWPR QMEEDFLKLSQ FNA RSVVRV
Sbjct: 213 IASRGGNILAADVFVPENKQVFYSRSDFVFDPVKWPRVQMEEDFLKLSQTFNAIRSVVRV 392
Query: 121 PALDPKYKIAVLASKQDHCLVDLLHGWQDGKLPVDITCVISNHHRDSNTHVIRFLERHGI 180
PALDPKYKIAVLASKQDHCLVDLLHGWQDG+LPVDITCVISNHHR SNTHVIRFLERHGI
Sbjct: 393 PALDPKYKIAVLASKQDHCLVDLLHGWQDGRLPVDITCVISNHHRGSNTHVIRFLERHGI 572
Query: 181 PYHCLSTTNENKREGEILELVQNTDFLVLARYMQATGMT*LTF---TMVCCHHSRVVIHL 237
PYH L TT ENKREGEIL+L+QNTD LVLARYMQ L ++ HH +
Sbjct: 573 PYHYLCTTKENKREGEILQLIQNTDILVLARYMQILSGNFLRSYGNDIINIHHGLLPSFX 752
Query: 238 NRLLRLV*N*LAQQVTLCPKNLTADQ*LNKWLR------EFHTEMTCRALCRNQKTLRNN 291
* + C K+L + + EF TE+TCRAL RNQKT RNN
Sbjct: 753 GW*SI*TGL*SRC*INRCNKSLCD*RT*RRTYN*TNG*GEFLTEITCRALYRNQKT*RNN 932
Query: 292 VFPRLLDPIVNFGYYL 307
FPRL D VNFG YL
Sbjct: 933 AFPRLSDLTVNFGCYL 980
>TC233275 similar to UP|O23579 (O23579) Formyltransferase purU homolog,
partial (40%)
Length = 685
Score = 152 bits (384), Expect = 2e-37
Identities = 90/137 (65%), Positives = 95/137 (68%), Gaps = 15/137 (10%)
Frame = +1
Query: 193 REGEILELVQNTDFLVLAR------YMQ---------ATGMT*LTFTMVCCHHSRVVIHL 237
REGEIL+LVQNT + + YMQ A G *LTFTMV CHHSRVVIHL
Sbjct: 1 REGEILQLVQNT*YFSTCKIYAALIYMQRYYLETF*GAMGTI*LTFTMVFCHHSRVVIHL 180
Query: 238 NRLLRLV*N*LAQQVTLCPKNLTADQ*LNKWLREFHTEMTCRALCRNQKTLRNNVFPRLL 297
NR LR V N*L QQVTL KNL D *LNKWLREF TE+TCRALCRNQKT RNN FPRL
Sbjct: 181 NRPLRQVLN*LVQQVTL*LKNLMLDL*LNKWLREFLTEITCRALCRNQKT*RNNAFPRLS 360
Query: 298 DPIVNFGYYLMRLRRLL 314
D VNFG YLM+ R LL
Sbjct: 361 DLTVNFGCYLMKQRGLL 411
>AW317195
Length = 466
Score = 106 bits (265), Expect(2) = 1e-36
Identities = 62/98 (63%), Positives = 68/98 (69%)
Frame = -2
Query: 217 GMT*LTFTMVCCHHSRVVIHLNRLLRLV*N*LAQQVTLCPKNLTADQ*LNKWLREFHTEM 276
GM *L F MVCC+H RVVI+LNRLL+ V N*L V + KNL D *LNKWLREF +
Sbjct: 297 GMI*LIFIMVCCYHLRVVIYLNRLLK*VLN*LV**VNM*LKNLM*DL*LNKWLREFFIGI 118
Query: 277 TCRALCRNQKTLRNNVFPRLLDPIVNFGYYLMRLRRLL 314
TCR L RN KT RNN F RLLD VNFG Y M+ R LL
Sbjct: 117 TCRVLYRN*KT*RNNAFLRLLDFTVNFGCYFMKKRGLL 4
Score = 64.7 bits (156), Expect(2) = 1e-36
Identities = 30/45 (66%), Positives = 37/45 (81%)
Frame = -1
Query: 169 THVIRFLERHGIPYHCLSTTNENKREGEILELVQNTDFLVLARYM 213
T+VIRFLER+GI Y+CL T E+KREGEIL L+ N DF+V+ RYM
Sbjct: 466 TYVIRFLERYGILYYCLCITTEHKREGEIL*LI*NXDFIVIVRYM 332
>AI441435
Length = 380
Score = 91.3 bits (225), Expect(3) = 8e-30
Identities = 51/73 (69%), Positives = 53/73 (71%)
Frame = +1
Query: 1 MGMVVRRVSQVLGLTNSNKIRNFSFKSLDLPPLPSPSLSHGIHVFHCPDAVGIVAKLSEC 60
M M R VLGL RN SFKSLD P SL+HGIHVFHCPDAVGIVAKLS+C
Sbjct: 79 MAMARRVPRNVLGLVK----RNMSFKSLD----PPSSLTHGIHVFHCPDAVGIVAKLSDC 234
Query: 61 IASRGGNILAADV 73
IASRGGNILAADV
Sbjct: 235 IASRGGNILAADV 273
Score = 43.9 bits (102), Expect(3) = 8e-30
Identities = 19/22 (86%), Positives = 20/22 (90%)
Frame = +2
Query: 88 FVFDPVKWPRKQMEEDFLKLSQ 109
FVFDPVKWPR Q+ EDFLKLSQ
Sbjct: 314 FVFDPVKWPRVQIGEDFLKLSQ 379
Score = 33.1 bits (74), Expect(3) = 8e-30
Identities = 13/14 (92%), Positives = 14/14 (99%)
Frame = +3
Query: 74 FVPQNKHVFYSRSD 87
FVP+NKHVFYSRSD
Sbjct: 273 FVPENKHVFYSRSD 314
>TC232723 similar to UP|O23579 (O23579) Formyltransferase purU homolog,
partial (12%)
Length = 495
Score = 53.5 bits (127), Expect = 1e-07
Identities = 25/34 (73%), Positives = 26/34 (75%)
Frame = +2
Query: 269 LREFHTEMTCRALCRNQKTLRNNVFPRLLDPIVN 302
LREF TE+TCRALCRNQ RNN FPRL D VN
Sbjct: 392 LREFLTEITCRALCRNQXXXRNNAFPRLSDLTVN 493
>BG239393 weakly similar to GP|16930491|gb| At1g06110/T21E18_13 {Arabidopsis
thaliana}, partial (9%)
Length = 424
Score = 28.9 bits (63), Expect = 3.2
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = -3
Query: 7 RVSQVLGLTNSNKIRNFSFKSLDLPPLPSPSLSH 40
R+ Q LGL + R +SL+LPP+P+PSL H
Sbjct: 113 RLWQCLGLLTY*ESR----ESLNLPPIPTPSLHH 24
>BU577659 weakly similar to GP|8809619|dbj| contains similarity to
salt-inducible protein~gene_id:MQL5.22 {Arabidopsis
thaliana}, partial (8%)
Length = 380
Score = 28.5 bits (62), Expect = 4.1
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -1
Query: 82 FYSRSDFVFDPVKWPRKQMEEDFLKLSQA 110
FY +S F+ PV W + DF+K+S A
Sbjct: 245 FYHQSPFLCMPVNWFTRSFSPDFIKISIA 159
>TC232261 weakly similar to UP|Q65198 (Q65198) PE199L, partial (7%)
Length = 502
Score = 28.1 bits (61), Expect = 5.4
Identities = 18/52 (34%), Positives = 24/52 (45%)
Frame = +2
Query: 126 KYKIAVLASKQDHCLVDLLHGWQDGKLPVDITCVISNHHRDSNTHVIRFLER 177
K K VL S+ C+ L P VIS HHRD +TH + ++R
Sbjct: 143 KQKKLVLGSRPPKCVNKCL-----SCKPCMAALVISPHHRDGHTHKAKTVQR 283
>CK606661
Length = 574
Score = 27.7 bits (60), Expect = 7.0
Identities = 19/56 (33%), Positives = 29/56 (50%), Gaps = 2/56 (3%)
Frame = +3
Query: 3 MVVRRVSQVLGLTNSNKIRNFSFKSLDLPPLPSPSLSHGIHVFH--CPDAVGIVAK 56
+V +S + GL+ + SFK L LPP S+ H +H CPD GI+++
Sbjct: 54 LVPLELSIIYGLSTLGNVPKKSFKPL-LPPEALLSIGH----YHTTCPDTEGIISQ 206
>BG239755
Length = 279
Score = 27.7 bits (60), Expect = 7.0
Identities = 10/22 (45%), Positives = 16/22 (72%)
Frame = +2
Query: 25 FKSLDLPPLPSPSLSHGIHVFH 46
F ++++ PL SPS HG+H+ H
Sbjct: 104 FHAMEVTPLSSPSPIHGLHLRH 169
>BU577461 similar to PIR|T11622|T11 extensin class 1 precursor - cowpea,
partial (24%)
Length = 354
Score = 27.3 bits (59), Expect = 9.2
Identities = 23/91 (25%), Positives = 41/91 (44%), Gaps = 5/91 (5%)
Frame = +2
Query: 155 DITCVISNHHRDSNTH----VIRFLERHGIPYHCLST-TNENKREGEILELVQNTDFLVL 209
++ +I++HH + H I L H + +H L+T TN + L+ T VL
Sbjct: 56 NLPTIITHHHHHLHLHHLPITINLLHHHHLHHHHLNTITNPLFLHLHLHHLI--TTIKVL 229
Query: 210 ARYMQATGMT*LTFTMVCCHHSRVVIHLNRL 240
++ + L T HH R+++HL +
Sbjct: 230 LHHLP---LLILPTTTNHLHHQRLILHLTTM 313
>BG650512
Length = 300
Score = 27.3 bits (59), Expect = 9.2
Identities = 14/35 (40%), Positives = 16/35 (45%)
Frame = +3
Query: 158 CVISNHHRDSNTHVIRFLERHGIPYHCLSTTNENK 192
C I+N H D THVI L YH + T K
Sbjct: 132 CWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAK 236
>AW317643 similar to GP|12484392|gb cellulase {Fragaria x ananassa}, partial
(25%)
Length = 511
Score = 27.3 bits (59), Expect = 9.2
Identities = 16/42 (38%), Positives = 19/42 (45%)
Frame = +3
Query: 24 SFKSLDLPPLPSPSLSHGIHVFHCPDAVGIVAKLSECIASRG 65
S K DL P+P SHG H+ C A V SE +G
Sbjct: 108 SLKLTDLDAFPTPRWSHGEHILVCKMANRAVWIYSEGTMMQG 233
>TC210418 similar to UP|Q9FMX6 (Q9FMX6) Gb|AAD21732.1, partial (36%)
Length = 598
Score = 27.3 bits (59), Expect = 9.2
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = +2
Query: 24 SFKSLDLPPLPSPSLSHGIHVFHCP 48
S S+ L P+P P LSH H H P
Sbjct: 32 STTSVSLSPMPPPPLSHARHYRHQP 106
>CB063867
Length = 426
Score = 27.3 bits (59), Expect = 9.2
Identities = 13/50 (26%), Positives = 21/50 (42%)
Frame = +1
Query: 163 HHRDSNTHVIRFLERHGIPYHCLSTTNENKREGEILELVQNTDFLVLARY 212
H N H F E H YH EN+ + ++++V F++ Y
Sbjct: 1 HQASKNVHEFEFSEFHQCNYHKYGNNYENELDQPVIDVVFCFIFILSISY 150
>TC225931
Length = 1469
Score = 27.3 bits (59), Expect = 9.2
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = +2
Query: 4 VVRRVSQVLGLTNSNKIRNFSFKSLDLPPLPSPSLSHGI 42
VV RV+Q LGL + +KIR P P P G+
Sbjct: 128 VVERVAQQLGLDDPSKIRLTPHNCYSQQPKPQPIKYRGV 244
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.333 0.144 0.459
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,074,223
Number of Sequences: 63676
Number of extensions: 239642
Number of successful extensions: 2303
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 2285
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2300
length of query: 314
length of database: 12,639,632
effective HSP length: 97
effective length of query: 217
effective length of database: 6,463,060
effective search space: 1402484020
effective search space used: 1402484020
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146650.9