BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146632.7 + phase: 0
(339 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC233295 similar to UP|Q7KVQ0 (Q7KVQ0) CG4038-PA, partial (6%) 75 4e-14
TC213978 62 4e-10
AW596916 40 0.002
TC222844 weakly similar to UP|Q9FFW4 (Q9FFW4) Similarity to heat... 39 0.003
AW756593 39 0.003
BG726934 36 0.022
AW203333 36 0.022
TC220267 similar to UP|Q6K235 (Q6K235) F-box-like protein, parti... 33 0.14
AW395660 33 0.14
BF325178 weakly similar to GP|29887975|gb| AKIN betagamma {Medic... 33 0.18
BM094601 33 0.24
TC213791 31 0.92
TC205860 similar to UP|Q9LVB9 (Q9LVB9) Emb|CAB83157.1, partial (... 31 0.92
TC221969 30 2.0
TC229968 similar to UP|O64797 (O64797) T1F15.5 protein, partial ... 29 2.7
TC223647 29 2.7
TC210605 weakly similar to UP|Q9FV01 (Q9FV01) SKP1 interacting p... 29 2.7
TC203883 weakly similar to GB|AAL15368.1|16323268|AY057738 At1g1... 29 2.7
TC205857 similar to UP|Q9LVB9 (Q9LVB9) Emb|CAB83157.1, partial (... 29 3.5
AW186414 29 3.5
>TC233295 similar to UP|Q7KVQ0 (Q7KVQ0) CG4038-PA, partial (6%)
Length = 557
Score = 75.1 bits (183), Expect = 4e-14
Identities = 49/167 (29%), Positives = 93/167 (55%), Gaps = 12/167 (7%)
Frame = +3
Query: 42 DRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSLRPRMTEINIDDTSYIHDRDAYDRY 101
DRIS LPD LL +ILSLLP KQ ++T +LS+RWR L P ++ ++ DD ++ +
Sbjct: 66 DRISHLPDVLLLQILSLLPTKQAVITGILSKRWRPLWPAVSVLDFDD-------ESSPEF 224
Query: 102 YHV-----IALFLFEMKIHH--PIMKTVTILSASPFFN--PLPLWL---GCLQVQHLDVT 149
+H A F++ + + H P ++ + A+P ++ + WL + + ++++
Sbjct: 225 HHPGGLTGFAEFVYSVLLLHDAPAIERFRLRCANPNYSARDIATWLCHVARRRAERVELS 404
Query: 150 SSATLCLCVPYKVLNSTALVVLKLNALTIDYVHRSSTNLPSLKILHL 196
S + + +P + + + V+KLN + ++ + S +LP LK+LH+
Sbjct: 405 LSLSRYVALPRCLFHCDTVSVMKLNGVFLNALASFSVSLPLLKVLHV 545
>TC213978
Length = 716
Score = 62.0 bits (149), Expect = 4e-10
Identities = 58/182 (31%), Positives = 89/182 (48%), Gaps = 10/182 (5%)
Frame = +1
Query: 168 LVVLKL------NALTIDYVHRSSTNLPSLKILHLTQVHFLKLKFLIKILSMSPLLEDLL 221
LVVLKL N +D+ S +LP L LHL Q L+ + + ++L SP LE L
Sbjct: 43 LVVLKLIGGLNRNPFPLDF---KSVDLPLLTTLHL-QSFILERRDMAELLRGSPNLEYLF 210
Query: 222 LKDLQVTDNTLAHDDAAALKPFPKLLRADVSESSISAFLLPLKLFYNVQFLRSQVLLQTL 281
+ + + A + PKLLRA ++ + PL++ NVQFLR ++
Sbjct: 211 VGHMYFSG------PEARFERLPKLLRATIAFGHV-----PLEVVNNVQFLRID-WMEHK 354
Query: 282 EQDFSTTQFLNLTHMDLIFHDGYY-WISLMKFICACPSLQTLTIHNIGGDY---DDHNNN 337
E+ +F NLTH++L + + W+ +++ I CP+LQ L I D DD +
Sbjct: 355 EEANLIPEFQNLTHLELGYSECTRDWVDVLEVIQRCPNLQILDIDMGSIDMTTRDDEGAD 534
Query: 338 WP 339
WP
Sbjct: 535 WP 540
>AW596916
Length = 455
Score = 39.7 bits (91), Expect = 0.002
Identities = 17/50 (34%), Positives = 30/50 (60%)
Frame = +3
Query: 42 DRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSLRPRMTEINIDDTSY 91
DR+S LPD +L I+ + K + T +LS+RW+ L R+T + + + +
Sbjct: 264 DRLSDLPDFVLLHIMKFMSMKHAVQTCVLSKRWKELWKRLTNLALHSSDF 413
Score = 36.6 bits (83), Expect = 0.017
Identities = 15/40 (37%), Positives = 24/40 (59%)
Frame = +3
Query: 1 MSGLPDELLCEILSFLPSEEFPSTSLLSKRWRRVWLGMPN 40
+S LPD +L I+ F+ + T +LSKRW+ +W + N
Sbjct: 270 LSDLPDFVLLHIMKFMSMKHAVQTCVLSKRWKELWKRLTN 389
>TC222844 weakly similar to UP|Q9FFW4 (Q9FFW4) Similarity to heat shock
transcription factor HSF30, partial (6%)
Length = 746
Score = 38.9 bits (89), Expect = 0.003
Identities = 17/46 (36%), Positives = 27/46 (57%)
Frame = +1
Query: 42 DRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSLRPRMTEINID 87
DRIS LPD LL +++ + K + T +LS+RW L +T + +
Sbjct: 496 DRISALPDSLLFHMINFMDTKSAVQTCVLSKRWNDLSKCLTNLTFN 633
Score = 35.4 bits (80), Expect = 0.037
Identities = 20/54 (37%), Positives = 30/54 (55%)
Frame = +1
Query: 1 MSGLPDELLCEILSFLPSEEFPSTSLLSKRWRRVWLGMPNADRISILPDELLCR 54
+S LPD LL +++F+ ++ T +LSKRW + + N S LP E L R
Sbjct: 502 ISALPDSLLFHMINFMDTKSAVQTCVLSKRWNDLSKCLTNLTFNSDLPSEGLDR 663
>AW756593
Length = 370
Score = 38.9 bits (89), Expect = 0.003
Identities = 17/66 (25%), Positives = 38/66 (56%)
Frame = +3
Query: 26 LLSKRWRRVWLGMPNADRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSLRPRMTEIN 85
L+ K ++L + D+IS LPD +L ++ + ++ + T +LS+RW +L R++ +
Sbjct: 15 LVVKMKEELFLPKDDRDKISELPDNILLHMMDFMDTREAVQTCVLSKRWNNLWKRLSTLL 194
Query: 86 IDDTSY 91
+ + +
Sbjct: 195FNTSKF 212
Score = 37.0 bits (84), Expect = 0.013
Identities = 20/63 (31%), Positives = 33/63 (51%), Gaps = 6/63 (9%)
Frame = +3
Query: 1 MSGLPDELLCEILSFLPSEEFPSTSLLSKRWRRVWLGMP----NADRISIL--PDELLCR 54
+S LPD +L ++ F+ + E T +LSKRW +W + N + + ++ LCR
Sbjct: 69 ISELPDNILLHMMDFMDTREAVQTCVLSKRWNNLWKRLSTLLFNTSKFESVFKINKFLCR 248
Query: 55 ILS 57
LS
Sbjct: 249 FLS 257
>BG726934
Length = 387
Score = 36.2 bits (82), Expect = 0.022
Identities = 22/54 (40%), Positives = 34/54 (62%), Gaps = 3/54 (5%)
Frame = +3
Query: 42 DRISILPDELLCRILSLLP--AKQIMVTSLLSRRWRSLRPRMTE-INIDDTSYI 92
DR +L DELL RILS LP ++Q SL+ +RW +L+ R+ + + D S++
Sbjct: 99 DRTLLLSDELLLRILSKLPDSSQQRNSNSLVCKRWLNLQGRLVRTLRVLDWSFV 260
Score = 29.3 bits (64), Expect = 2.7
Identities = 17/30 (56%), Positives = 20/30 (66%), Gaps = 2/30 (6%)
Frame = +3
Query: 4 LPDELLCEILSFLP--SEEFPSTSLLSKRW 31
L DELL ILS LP S++ S SL+ KRW
Sbjct: 114 LSDELLLRILSKLPDSSQQRNSNSLVCKRW 203
>AW203333
Length = 418
Score = 36.2 bits (82), Expect = 0.022
Identities = 17/39 (43%), Positives = 23/39 (58%)
Frame = +1
Query: 1 MSGLPDELLCEILSFLPSEEFPSTSLLSKRWRRVWLGMP 39
+S LP +L +ILS +P E+ TS+LSK W W P
Sbjct: 91 ISTLPKTILHDILSRMPEEDAVRTSVLSKSWAEAWSTYP 207
Score = 32.0 bits (71), Expect = 0.41
Identities = 16/42 (38%), Positives = 24/42 (57%)
Frame = +1
Query: 33 RVWLGMPNADRISILPDELLCRILSLLPAKQIMVTSLLSRRW 74
++W D IS LP +L ILS +P + + TS+LS+ W
Sbjct: 58 KLWS*EMERDMISTLPKTILHDILSRMPEEDAVRTSVLSKSW 183
>TC220267 similar to UP|Q6K235 (Q6K235) F-box-like protein, partial (7%)
Length = 586
Score = 33.5 bits (75), Expect = 0.14
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +2
Query: 44 ISILPDELLCRILSLLPAKQIMVTSLLSRRWRSL 77
+ +LPD+LL RIL+ LP I +S+RW +
Sbjct: 329 LDVLPDDLLERILAYLPIASIFRAGCVSKRWHEI 430
Score = 30.0 bits (66), Expect = 1.6
Identities = 13/31 (41%), Positives = 18/31 (57%)
Frame = +2
Query: 4 LPDELLCEILSFLPSEEFPSTSLLSKRWRRV 34
LPD+LL IL++LP +SKRW +
Sbjct: 338 LPDDLLERILAYLPIASIFRAGCVSKRWHEI 430
>AW395660
Length = 381
Score = 33.5 bits (75), Expect = 0.14
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +3
Query: 37 GMPNADRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSL 77
GM + + LPDEL+ ILS LP K ++ + + W SL
Sbjct: 234 GMSESLPLPFLPDELVVEILSRLPVKSLLQFRCVCKSWMSL 356
>BF325178 weakly similar to GP|29887975|gb| AKIN betagamma {Medicago
truncatula}, partial (14%)
Length = 361
Score = 33.1 bits (74), Expect = 0.18
Identities = 15/41 (36%), Positives = 26/41 (62%)
Frame = +2
Query: 159 PYKVLNSTALVVLKLNALTIDYVHRSSTNLPSLKILHLTQV 199
P+K L + AL VL+ ++T+ +H SS + ++LHLT +
Sbjct: 149 PHKYLKNIALKVLQNRSITVPIIHSSSEDNAYAQLLHLTSL 271
>BM094601
Length = 429
Score = 32.7 bits (73), Expect = 0.24
Identities = 17/33 (51%), Positives = 20/33 (60%)
Frame = +3
Query: 2 SGLPDELLCEILSFLPSEEFPSTSLLSKRWRRV 34
+ L DELL EI LPS S SL+ KRW R+
Sbjct: 15 NSLYDELLQEIFQKLPSSSSSSVSLVCKRWLRL 113
Score = 31.2 bits (69), Expect = 0.70
Identities = 33/133 (24%), Positives = 57/133 (42%), Gaps = 1/133 (0%)
Frame = +3
Query: 47 LPDELLCRILSLLPAKQIMVTSLLSRRWRSL-RPRMTEINIDDTSYIHDRDAYDRYYHVI 105
L DELL I LP+ SL+ +RW L R T +++ T+ ++ +I
Sbjct: 21 LYDELLQEIFQKLPSSSSSSVSLVCKRWLRLHRSSTTSLSLRLTT---------PHFSLI 173
Query: 106 ALFLFEMKIHHPIMKTVTILSASPFFNPLPLWLGCLQVQHLDVTSSATLCLCVPYKVLNS 165
L HHP + ++++ + P F+ P L + ++ S + V L S
Sbjct: 174 P-SLSSFLSHHPFLSSLSLSLSPPLFSLSPHLLSLIISPFSNLLSLSLTPAPVSLSSLLS 350
Query: 166 TALVVLKLNALTI 178
+ +LN+L I
Sbjct: 351 LSASCPRLNSLRI 389
>TC213791
Length = 667
Score = 30.8 bits (68), Expect = 0.92
Identities = 13/39 (33%), Positives = 23/39 (58%)
Frame = -1
Query: 248 RADVSESSISAFLLPLKLFYNVQFLRSQVLLQTLEQDFS 286
R D+++S +PL FYN+ ++ L+T ++DFS
Sbjct: 244 RLDLNQSYSDITSMPLSFFYNIYIIQMVYQLRTTKEDFS 128
>TC205860 similar to UP|Q9LVB9 (Q9LVB9) Emb|CAB83157.1, partial (10%)
Length = 441
Score = 30.8 bits (68), Expect = 0.92
Identities = 23/86 (26%), Positives = 38/86 (43%)
Frame = +3
Query: 114 IHHPIMKTVTILSASPFFNPLPLWLGCLQVQHLDVTSSATLCLCVPYKVLNSTALVVLKL 173
IHHP+ T IL A W L +QHL + + LC+ Y+ +T+L+ +L
Sbjct: 192 IHHPLDSTQKILFALLLHQS---WPRTLHLQHLLIHQHIQMDLCLNYQTS*ATSLLSKQL 362
Query: 174 NALTIDYVHRSSTNLPSLKILHLTQV 199
I + + P K +H+ +
Sbjct: 363 ----IPHAFLNFIQFPLTKHIHVPSI 428
>TC221969
Length = 581
Score = 29.6 bits (65), Expect = 2.0
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +1
Query: 1 MSGLPDELLCEILSFLPSEEFPSTSLLSKRWRRV 34
+SGLPD+L L +P E L+ KRW R+
Sbjct: 196 LSGLPDDLAIACLIRVPRIEHRKLHLVCKRWHRL 297
>TC229968 similar to UP|O64797 (O64797) T1F15.5 protein, partial (13%)
Length = 902
Score = 29.3 bits (64), Expect = 2.7
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +3
Query: 1 MSGLPDELLCEILSFLPSEEFPSTSLLSKRWR 32
+ GLPD++ L+ +P FP+ ++ K WR
Sbjct: 711 LPGLPDDVAEYCLALVPRSNFPAMGVVCKGWR 806
>TC223647
Length = 448
Score = 29.3 bits (64), Expect = 2.7
Identities = 21/64 (32%), Positives = 33/64 (50%), Gaps = 2/64 (3%)
Frame = +3
Query: 20 EFPSTSLLSKRWR--RVWLGMPNADRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSL 77
E PS+S S+R +V +S+L ++LL IL+ LPA + +S+ W SL
Sbjct: 36 EKPSSSSSSRRKSSTKVKASTDANTNLSMLNEDLLQNILARLPALHFASAACVSKSWNSL 215
Query: 78 RPRM 81
R+
Sbjct: 216CNRI 227
>TC210605 weakly similar to UP|Q9FV01 (Q9FV01) SKP1 interacting partner 4,
partial (12%)
Length = 661
Score = 29.3 bits (64), Expect = 2.7
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +1
Query: 40 NADRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSL 77
N+ I LPD++ L+ +P K V +S+RWR+L
Sbjct: 190 NSPIICGLPDDISLMCLARIPRKYHSVMKCVSKRWRNL 303
>TC203883 weakly similar to GB|AAL15368.1|16323268|AY057738 At1g15670/F7H2_1
{Arabidopsis thaliana;} , partial (61%)
Length = 1668
Score = 29.3 bits (64), Expect = 2.7
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = +3
Query: 1 MSGLPDELLCEILSFLPSEEFPSTSLLSKRW 31
+SGLP+++ + L +P E+FP+ + + K W
Sbjct: 216 ISGLPEDVARDCLIRIPYEQFPAVASVCKGW 308
>TC205857 similar to UP|Q9LVB9 (Q9LVB9) Emb|CAB83157.1, partial (28%)
Length = 1056
Score = 28.9 bits (63), Expect = 3.5
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = +1
Query: 114 IHHPIMKTVTILSASPFFNPLPLWLGCLQVQHLDVTSSATLCLCVPYKVLNSTALV 169
IHHP+ T IL A W L +QHL + + LC+ Y+ +T+L+
Sbjct: 175 IHHPLDSTQKILFALLLHQS---WPRTLHLQHLLIHQHIQMDLCLNYQTS*ATSLL 333
>AW186414
Length = 445
Score = 28.9 bits (63), Expect = 3.5
Identities = 13/37 (35%), Positives = 20/37 (53%)
Frame = +1
Query: 1 MSGLPDELLCEILSFLPSEEFPSTSLLSKRWRRVWLG 37
+ GLPD + L+++P P L+S+ WR V G
Sbjct: 211 IEGLPDAVAIRCLAWVPFYLHPKLELVSRAWRAVVRG 321
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.326 0.140 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,145,203
Number of Sequences: 63676
Number of extensions: 294801
Number of successful extensions: 2525
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 2460
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2522
length of query: 339
length of database: 12,639,632
effective HSP length: 98
effective length of query: 241
effective length of database: 6,399,384
effective search space: 1542251544
effective search space used: 1542251544
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146632.7