BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146588.13 - phase: 0
(107 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC218123 132 2e-32
TC233799 similar to GB|AAN28849.1|23505979|AY143910 At4g27020/F1... 119 3e-28
TC228816 27 1.2
TC211177 weakly similar to UP|Q9LW63 (Q9LW63) Emb|CAB37460.1, pa... 27 1.5
TC228045 similar to UP|Q9LVI4 (Q9LVI4) Gb|AAF09073.1 (AT3g17860/... 26 3.4
BI317603 25 5.7
BI702109 25 5.7
TC227815 similar to UP|RIR2_TOBAC (P49730) Ribonucleoside-diphos... 25 5.7
TC219587 similar to PIR|T49181|T49181 cyclophylin-like protein -... 25 5.7
AW832477 similar to GP|29561808|emb SI:zC215I13.5, partial (18%) 25 5.7
TC224342 25 5.7
TC206015 similar to UP|Q9AXU5 (Q9AXU5) Pathogen-inducible alpha-... 25 7.5
TC227325 UP|O04133 (O04133) SRC2, complete 24 9.8
TC219767 24 9.8
BE824356 24 9.8
TC225900 homologue to UP|ODPA_PEA (P52902) Pyruvate dehydrogenas... 24 9.8
TC203811 similar to GB|BAA12349.2|9081770|D84417 monodehydroasco... 24 9.8
TC216877 weakly similar to UP|Q753E9 (Q753E9) AFR365Cp, partial ... 24 9.8
TC209355 similar to UP|O82694 (O82694) MdMADS9 protein (Fragment... 24 9.8
>TC218123
Length = 1048
Score = 132 bits (333), Expect = 2e-32
Identities = 65/79 (82%), Positives = 67/79 (84%), Gaps = 3/79 (3%)
Frame = +2
Query: 1 MPSGMLGTLLSQIDAVPLFSDTAWGHKVNLDFLNKHMGAT---RSQPWRATTDPADVHSG 57
MPSGMLGTLLS ID +PLFS+T WG K NLDFL KHMGAT RSQPWRA DPADVHSG
Sbjct: 812 MPSGMLGTLLSLIDVLPLFSNTGWGQKANLDFLKKHMGATFEKRSQPWRAYIDPADVHSG 991
Query: 58 DFLAVSKICGRWGGFETLE 76
DFLAVSKI GRWGGFETLE
Sbjct: 992 DFLAVSKIRGRWGGFETLE 1048
>TC233799 similar to GB|AAN28849.1|23505979|AY143910 At4g27020/F10M23_360
{Arabidopsis thaliana;} , partial (26%)
Length = 417
Score = 119 bits (297), Expect = 3e-28
Identities = 58/93 (62%), Positives = 65/93 (69%), Gaps = 6/93 (6%)
Frame = +1
Query: 20 SDTAWGHKVNLDFLNKHMGAT---RSQPWRATTDPADVHSGDFLAVSKICGRWGGFETLE 76
++T WG N+ FL KHMGA+ R QPW D+HSGDFLAVSKI GRWG FETLE
Sbjct: 1 ANTGWGENSNIKFLEKHMGASFEVRPQPWVTNVSADDIHSGDFLAVSKIRGRWGAFETLE 180
Query: 77 KLVT---ADHAAVCLKDEMGSLWVGESGHENEK 106
K V+ A H AVCL+D G LWVGESGHENEK
Sbjct: 181 KWVSGAYAGHTAVCLRDSSGKLWVGESGHENEK 279
>TC228816
Length = 520
Score = 27.3 bits (59), Expect = 1.2
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = -2
Query: 46 RATTDPADVHSGDFLAVSKICGRWGGFETLEKL 78
R +DP + + A ++ICG W G E +E L
Sbjct: 279 RFGSDPGERRNSRRRAPTRICGGWSGKECMESL 181
>TC211177 weakly similar to UP|Q9LW63 (Q9LW63) Emb|CAB37460.1, partial (17%)
Length = 823
Score = 26.9 bits (58), Expect = 1.5
Identities = 11/30 (36%), Positives = 17/30 (56%), Gaps = 3/30 (10%)
Frame = +2
Query: 53 DVHSGDFLAVSKIC---GRWGGFETLEKLV 79
D H GD++ +S +C GRW L K++
Sbjct: 152 DSHGGDYVILSNLCARNGRWDDVNHLRKMM 241
>TC228045 similar to UP|Q9LVI4 (Q9LVI4) Gb|AAF09073.1 (AT3g17860/MEB5_8),
partial (25%)
Length = 1625
Score = 25.8 bits (55), Expect = 3.4
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = -1
Query: 49 TDPADVHSGDFLAVSK 64
+DP ++HSGDFL + K
Sbjct: 1259 SDPEELHSGDFLLIYK 1212
>BI317603
Length = 335
Score = 25.0 bits (53), Expect = 5.7
Identities = 12/33 (36%), Positives = 20/33 (60%), Gaps = 2/33 (6%)
Frame = -1
Query: 29 NLDFLNKHMGATRSQPW--RATTDPADVHSGDF 59
N+DF N H+ TR+ ATT+P + ++ +F
Sbjct: 323 NIDFSNTHISRTRTSLLLSPATTEPFESYASNF 225
>BI702109
Length = 379
Score = 25.0 bits (53), Expect = 5.7
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -1
Query: 81 ADHAAVCLKDEMGSLWVGESGHE 103
ADH+ KDE +LW G S H+
Sbjct: 109 ADHS*SS*KDERRNLWCGISNHD 41
>TC227815 similar to UP|RIR2_TOBAC (P49730) Ribonucleoside-diphosphate
reductase small chain (Ribonucleotide reductase) (R2
subunit) , complete
Length = 1407
Score = 25.0 bits (53), Expect = 5.7
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = -2
Query: 46 RATTDPADVHSGDFLAVSKICGRWGG 71
R+T+ + FL +S+ICG W G
Sbjct: 341 RSTSSAVQNEASAFLYISQICGYWIG 264
>TC219587 similar to PIR|T49181|T49181 cyclophylin-like protein - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (19%)
Length = 1344
Score = 25.0 bits (53), Expect = 5.7
Identities = 10/26 (38%), Positives = 12/26 (45%)
Frame = -1
Query: 45 WRATTDPADVHSGDFLAVSKICGRWG 70
WRA + PA H F+ C WG
Sbjct: 1068 WRARSRPAARHGSPFIITFWWCSIWG 991
>AW832477 similar to GP|29561808|emb SI:zC215I13.5, partial (18%)
Length = 289
Score = 25.0 bits (53), Expect = 5.7
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = -3
Query: 46 RATTDPADVHSGDFLAVSKICGRWGG 71
R+T+ + FL +S+ICG W G
Sbjct: 86 RSTSSAVQNEASAFLYISQICGYWIG 9
>TC224342
Length = 717
Score = 25.0 bits (53), Expect = 5.7
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = +3
Query: 58 DFLAVSKICGRWG 70
DF +S ICG+WG
Sbjct: 144 DFAFISNICGQWG 182
>TC206015 similar to UP|Q9AXU5 (Q9AXU5) Pathogen-inducible
alpha-dioxygenase, partial (61%)
Length = 1494
Score = 24.6 bits (52), Expect = 7.5
Identities = 9/19 (47%), Positives = 11/19 (57%)
Frame = +3
Query: 69 WGGFETLEKLVTADHAAVC 87
W G TL+ L +H AVC
Sbjct: 66 WAGVSTLQALFIQEHNAVC 122
>TC227325 UP|O04133 (O04133) SRC2, complete
Length = 1235
Score = 24.3 bits (51), Expect = 9.8
Identities = 11/25 (44%), Positives = 12/25 (48%)
Frame = +1
Query: 45 WRATTDPADVHSGDFLAVSKICGRW 69
W AT PA GD + VS G W
Sbjct: 634 WYATPSPAAAICGDGIWVSTSAGAW 708
>TC219767
Length = 1421
Score = 24.3 bits (51), Expect = 9.8
Identities = 13/45 (28%), Positives = 17/45 (36%)
Frame = +2
Query: 26 HKVNLDFLNKHMGATRSQPWRATTDPADVHSGDFLAVSKICGRWG 70
H + F N S A +D + H GD VS + WG
Sbjct: 185 HNASFFFFNSFTSGISSD---AESDDENHHKGDTFTVSYLINSWG 310
>BE824356
Length = 634
Score = 24.3 bits (51), Expect = 9.8
Identities = 14/37 (37%), Positives = 20/37 (53%), Gaps = 1/37 (2%)
Frame = -1
Query: 40 TRSQP-WRATTDPADVHSGDFLAVSKICGRWGGFETL 75
T+ QP WR P + SGDF S++ GG ++L
Sbjct: 322 TKLQPIWRVQITPRYMFSGDFFLDSRL----GGMQSL 224
>TC225900 homologue to UP|ODPA_PEA (P52902) Pyruvate dehydrogenase E1
component alpha subunit, mitochondrial precursor
(PDHE1-A) , partial (54%)
Length = 1042
Score = 24.3 bits (51), Expect = 9.8
Identities = 12/34 (35%), Positives = 17/34 (49%)
Frame = +2
Query: 34 NKHMGATRSQPWRATTDPADVHSGDFLAVSKICG 67
N H G ++ WRA PA GD++ K+ G
Sbjct: 149 NNHYGMGTAE-WRAAKSPAYYKRGDYVPGLKVDG 247
>TC203811 similar to GB|BAA12349.2|9081770|D84417 monodehydroascorbate
reductase {Arabidopsis thaliana;} , partial (61%)
Length = 1316
Score = 24.3 bits (51), Expect = 9.8
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = +1
Query: 21 DTAWGHKVNLDFLN 34
D W HKV+L+FLN
Sbjct: 1090 DLIWCHKVSLNFLN 1131
>TC216877 weakly similar to UP|Q753E9 (Q753E9) AFR365Cp, partial (17%)
Length = 511
Score = 24.3 bits (51), Expect = 9.8
Identities = 26/80 (32%), Positives = 33/80 (40%), Gaps = 4/80 (5%)
Frame = -2
Query: 1 MPSGMLGTLLSQIDAVPLFSDTAWGHKVNLDFLNKHMGATRSQPWRATTDPADV-HSGDF 59
MP+ L + I L ++ AW HK+ L L H TRS PA V G
Sbjct: 351 MPASGLAFWVFLIHLNSLETEVAWQHKLCLHHLCLHF--TRSHNPTQPKSPATVSDDGSE 178
Query: 60 LAVSKICGR-W--GGFETLE 76
+A G W GG E +E
Sbjct: 177 VATPHCTGADWDRGGVEVVE 118
>TC209355 similar to UP|O82694 (O82694) MdMADS9 protein (Fragment), partial
(14%)
Length = 388
Score = 24.3 bits (51), Expect = 9.8
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = +2
Query: 9 LLSQIDAVPLFSDTAWGHKVNLDFLNKHMGATRSQ 43
L S++DAV L S +W HK +KH+ +SQ
Sbjct: 122 LYSRVDAVKLGSVFSWRHK------SKHLTKGQSQ 208
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.134 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,136,298
Number of Sequences: 63676
Number of extensions: 60582
Number of successful extensions: 220
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 217
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 217
length of query: 107
length of database: 12,639,632
effective HSP length: 83
effective length of query: 24
effective length of database: 7,354,524
effective search space: 176508576
effective search space used: 176508576
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)
Medicago: description of AC146588.13