BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146587.10 + phase: 0 /pseudo
(1157 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC222786 similar to UP|Q8YYM0 (Q8YYM0) Alr0828 protein, partial ... 451 e-127
BE609771 156 6e-38
TC208436 weakly similar to UP|Q6UK63 (Q6UK63) Rac-binding compon... 128 1e-29
TC208435 70 8e-24
AW620333 similar to GP|21591633|gb| cleavage stimulation factor ... 33 0.55
TC205974 homologue to UP|Q94KS2 (Q94KS2) TGF-beta receptor-inter... 31 3.6
TC234988 similar to UP|Q9LNA8 (Q9LNA8) F5O11.10, partial (16%) 30 4.7
TC206102 30 6.1
TC208733 weakly similar to UP|Q9VNX8 (Q9VNX8) CG7414-PA (LD24903... 30 8.0
>TC222786 similar to UP|Q8YYM0 (Q8YYM0) Alr0828 protein, partial (12%)
Length = 926
Score = 451 bits (1161), Expect = e-127
Identities = 231/257 (89%), Positives = 237/257 (91%), Gaps = 17/257 (6%)
Frame = +3
Query: 269 ERHILLRVLPVLVVLVTSSEKDSESLYKRVKINRLINIFKNEAVIPAFPDLHLSPAAIMK 328
ERHILLRVLPVLVVLVTSSEKDSESLYKRVKINRLINIFKNEAVIPAFPDLHLSPAAI+K
Sbjct: 3 ERHILLRVLPVLVVLVTSSEKDSESLYKRVKINRLINIFKNEAVIPAFPDLHLSPAAILK 182
Query: 329 ELSTYFPKFSSQTRLLTLAAPHELPP------------LNHIGAVRAEHDDFTIRFASAM 376
ELSTYFPKFSSQTRLLTL APHELPP +NHIGA+RAEHDDF IRFASAM
Sbjct: 183 ELSTYFPKFSSQTRLLTLPAPHELPPREAQEYQRHYLIINHIGAIRAEHDDFVIRFASAM 362
Query: 377 NQ-----STDGSDVDWSKEVKGNMYDMIVEGFQLLSRWTARIWEQCAWKFSRPCKDASPS 431
NQ STDGSDV+WSKEVKGNMYDMIVEGFQLLSRWTARIWEQCAWKFSRPCKDASPS
Sbjct: 363 NQLLLLKSTDGSDVEWSKEVKGNMYDMIVEGFQLLSRWTARIWEQCAWKFSRPCKDASPS 542
Query: 432 FSDYEKVVRYNYSAEERKALVELVSCIKSVGSMMQRCDTLVADALWETIHAEVQDFVQNT 491
FSDYEKVVRYNYSAEERKALVELVS IKSVGSMMQRCDTLVADALWETIH+EVQDFVQNT
Sbjct: 543 FSDYEKVVRYNYSAEERKALVELVSYIKSVGSMMQRCDTLVADALWETIHSEVQDFVQNT 722
Query: 492 LASMLRTTFRKKKDLSR 508
LA+MLRTTFRKKKDLSR
Sbjct: 723 LATMLRTTFRKKKDLSR 773
>BE609771
Length = 458
Score = 156 bits (394), Expect = 6e-38
Identities = 87/139 (62%), Positives = 99/139 (70%)
Frame = +2
Query: 807 FILCNTTQRFIRSSKTVPVQKPSIPSAKPSFYCGTQPS*SYIKQDYFA*TNDNRITRIHA 866
+IL NT+ F S+ +P+ + S+ S*S+IKQDYFA*TND RITR A
Sbjct: 71 WILWNTSHVFYCSTSGIPIITLAY*SS----------S*SHIKQDYFA*TNDYRITRFFA 220
Query: 867 KINRPSSF*WRRDRLCKTC*RTS*LGDKIGAESRGSSRYKRDWQCIILDGASRYCVERNR 926
KIN SSF WR DRLC+T * S*LGDKI ES GSS +KRDW+CIILDGASRYCVER
Sbjct: 221 KINWASSFRWRCDRLCETR*GAS*LGDKIRTES*GSSWHKRDWKCIILDGASRYCVERKG 400
Query: 927 YNEFHANCSLAGLASWSRW 945
+ FHANCSLAGLASWSRW
Sbjct: 401 FYGFHANCSLAGLASWSRW 457
>TC208436 weakly similar to UP|Q6UK63 (Q6UK63) Rac-binding component of SCAR
regulatory complex, partial (3%)
Length = 1000
Score = 128 bits (322), Expect = 1e-29
Identities = 64/70 (91%), Positives = 66/70 (93%)
Frame = +2
Query: 1053 GYLEESAQVQSSSPERLGDSVAWGGCTIIYLLGQQLHFELFDFSYQILNIAEVEAASVVQ 1112
GYLEESAQV S+S ERLGDSVAWGGCTIIYLLGQQLHFELFDFSYQILNIAEVEAASV+Q
Sbjct: 158 GYLEESAQVPSNSHERLGDSVAWGGCTIIYLLGQQLHFELFDFSYQILNIAEVEAASVMQ 337
Query: 1113 TQKNSHFAVQ 1122
T KNS FAVQ
Sbjct: 338 THKNSQFAVQ 367
Score = 121 bits (303), Expect = 2e-27
Identities = 78/183 (42%), Positives = 97/183 (52%), Gaps = 25/183 (13%)
Frame = +1
Query: 1000 EYWKCSGVCACFHKCCIR*IL**MECCAQDRLH*HYNFKRFLPDIQWSPNCT*GYLEESA 1059
E+WKC+GVC CFH CC+ +L**MECC Q+RLH*HYNF RFL ++QWSPN G + SA
Sbjct: 7 EHWKCAGVCTCFH*CCVGQVL**MECCTQNRLH*HYNF*RFLSNLQWSPNWILGRVCPSA 186
Query: 1060 QVQSSSPERLG-------DSVAWGGCTI----IYLLGQQLHFELFDFSYQ---ILNIAEV 1105
S R + AW +++ Q Y L I
Sbjct: 187 IK*S*KAGRFSCLGGLHYNLFAWAAAAF*AV*LFISDPQYRRSGGCICYANT*ELTICRA 366
Query: 1106 EAASVVQTQKNSH-----------FAVQP*RKDSMRY*AKWCSITSYKI*KHSFSI*NSA 1154
S++ + + S + P*RKDS+ Y*AKWCS TSYKI* HSFSI*N+A
Sbjct: 367 GMGSLIGSNEKSKEIK*PCIFYA*STLPP*RKDSLCY*AKWCSNTSYKI**HSFSI*NTA 546
Query: 1155 TER 1157
ER
Sbjct: 547 AER 555
>TC208435
Length = 740
Score = 70.5 bits (171), Expect(2) = 8e-24
Identities = 36/47 (76%), Positives = 39/47 (82%)
Frame = +2
Query: 1076 GGCTIIYLLGQQLHFELFDFSYQILNIAEVEAASVVQTQKNSHFAVQ 1122
G TIIYLL QLH ELFDFSYQILNIAEVEAASV+QT KNS +V+
Sbjct: 2 GXXTIIYLLXXQLHXELFDFSYQILNIAEVEAASVMQTHKNSQXSVK 142
Score = 59.7 bits (143), Expect(2) = 8e-24
Identities = 30/35 (85%), Positives = 32/35 (90%)
Frame = +1
Query: 1123 P*RKDSMRY*AKWCSITSYKI*KHSFSI*NSATER 1157
P*RKDS+RY*AKWCS TSYKI* HSFSI*N+A ER
Sbjct: 226 P*RKDSLRY*AKWCSNTSYKI**HSFSI*NTAAER 330
>AW620333 similar to GP|21591633|gb| cleavage stimulation factor 64
{Arabidopsis thaliana}, partial (14%)
Length = 405
Score = 33.5 bits (75), Expect = 0.55
Identities = 27/103 (26%), Positives = 50/103 (48%)
Frame = +2
Query: 10 AALSTFSLEDEQPEVQGPGVWVSTERGATESPIEYCDVAAYRLSLSEDTKALNQLSSLTQ 69
AA+ T +L Q +Q + ++ P+ + ++S S+ T+ +++L +
Sbjct: 119 AAVMTAALGGAQFGIQSNQNSLQSQSALAHDPLT---LHLAKMSRSQLTEIISELKGMAT 289
Query: 70 EGKEMASVLYTYRSCVKALPQLPDSMKQSQADLYLETYQVLDL 112
+ KE+A L R PQLP ++ Q+Q L + T QVL +
Sbjct: 290 QNKELARQLLLSR------PQLPKALFQAQIMLGMVTSQVLQM 400
>TC205974 homologue to UP|Q94KS2 (Q94KS2) TGF-beta receptor-interacting protein
1, partial (57%)
Length = 783
Score = 30.8 bits (68), Expect = 3.6
Identities = 23/71 (32%), Positives = 32/71 (44%), Gaps = 2/71 (2%)
Frame = +2
Query: 944 RWTNITFSRWWR*PCCQSL--*IYSCCNGFIPRLPITCFFPHHVKTSRSCRSFI*G*LEY 1001
RW TF W+ * CQ+L *+ R P C + HV +S SC Y
Sbjct: 191 RW--FTFPYWFP**VCQALGY*VIDSYQDLCHRTPSQCSY--HVTSS*SCG--------Y 334
Query: 1002 WKCSGVCACFH 1012
W+ SG +C++
Sbjct: 335 WRWSGCLSCYN 367
>TC234988 similar to UP|Q9LNA8 (Q9LNA8) F5O11.10, partial (16%)
Length = 428
Score = 30.4 bits (67), Expect = 4.7
Identities = 19/50 (38%), Positives = 29/50 (58%), Gaps = 8/50 (16%)
Frame = +3
Query: 679 LYDEIEAEV-DHCFDIFVA-----RLCETIF--TYYKSWAASELLDPTFL 720
L+DE+ A+V D C +A +L E F Y+ +WAA+ +LDP +L
Sbjct: 204 LWDELRAKVKDWCSKFHIAEGVVEKLVERRFKKNYHPAWAAAYILDPLYL 353
>TC206102
Length = 982
Score = 30.0 bits (66), Expect = 6.1
Identities = 17/58 (29%), Positives = 25/58 (42%)
Frame = +3
Query: 646 ESPNSGLLESVLMPFDIYNDSAQQALVLLKQRFLYDEIEAEVDHCFDIFVARLCETIF 703
ESPNSG S P + N++ + L+ + Y A H F I+ C+ F
Sbjct: 342 ESPNSGASSSFTQPMEDMNENEETDLLSISWNQDYGCFAAGTSHGFRIYNCEPCKETF 515
>TC208733 weakly similar to UP|Q9VNX8 (Q9VNX8) CG7414-PA (LD24903p), partial
(13%)
Length = 1217
Score = 29.6 bits (65), Expect = 8.0
Identities = 19/59 (32%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
Frame = +1
Query: 5 VEEAIAALSTFSLEDEQPEVQGP--GVWVSTERGATESPIEYCDVAAYRLSLSEDTKAL 61
+ E I +L+ LED++P QGP ST+ +T P + AAYR +++ A+
Sbjct: 733 IAELIKSLNLVQLEDKKPSGQGPPKSTQTSTKASSTNPPTQ--KPAAYRPPHAKNASAI 903
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.331 0.140 0.442
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,041,405
Number of Sequences: 63676
Number of extensions: 869729
Number of successful extensions: 5102
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 5025
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5098
length of query: 1157
length of database: 12,639,632
effective HSP length: 108
effective length of query: 1049
effective length of database: 5,762,624
effective search space: 6044992576
effective search space used: 6044992576
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 64 (29.3 bits)
Medicago: description of AC146587.10