BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146583.2 + phase: 0 /pseudo
(577 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC231258 similar to UP|Q886T6 (Q886T6) Sensory box histidine kin... 128 6e-30
TC232867 similar to UP|Q9MA68 (Q9MA68) F2J6.13 protein, partial ... 119 5e-27
TC232398 75 1e-24
TC222094 64 1e-21
BQ133845 97 3e-20
CF922341 36 0.041
TC231541 similar to UP|Q8LCH9 (Q8LCH9) Thioredoxin-like protein,... 27 0.30
TC215407 weakly similar to GB|CAD29448.2|28804246|MMU440757 IL-1... 30 3.9
TC217698 similar to UP|Q89GY6 (Q89GY6) Blr6209 protein, partial ... 30 3.9
TC227422 similar to UP|P92973 (P92973) CCA1 (MYB-related transcr... 29 5.0
TC213120 similar to UP|Q9JL44 (Q9JL44) ATP-binding cassette prot... 29 5.0
TC209544 similar to UP|Q8W040 (Q8W040) Dihydrofolate synthetase ... 29 6.6
BG550748 29 6.6
>TC231258 similar to UP|Q886T6 (Q886T6) Sensory box histidine kinase/response
regulator, partial (3%)
Length = 1374
Score = 128 bits (322), Expect = 6e-30
Identities = 74/191 (38%), Positives = 105/191 (54%), Gaps = 5/191 (2%)
Frame = +2
Query: 3 QFSGSPTEDPNLHISSFLRLSGTIKE---NQEAVRLHLFPFSLRDRASAWFHSLEVGSIT 59
+F G EDP+ H+ F + T+K ++ + L FP SL A W + L SI
Sbjct: 551 KFHGLVGEDPHKHLKEFHIVCSTMKPPDVQEDHIFLKAFPHSLEGVAKDWLYYLAPRSIF 730
Query: 60 SWDDMRRAFLARFFPPSKTAKLRDQITRFNQKDGESLYEAWEHFKEMLRLCPHHGLEKWL 119
+WDD++R FL +FFP S+T +R I+ Q ESLYE WE FK+ CPHH + K L
Sbjct: 731 NWDDLKRVFLEKFFPASRTTTIRKDISGIRQLSRESLYEYWERFKKSCASCPHHQISKQL 910
Query: 120 IVHTFYNGLSDTTKMSVDAAAGGALMNKNYTEAYALIEDMAQNHYQWT--NERAVTTPTP 177
++ FY LS+ + +DAA+GGAL + TEA LI+ MA N Q++ N+ V
Sbjct: 911 LL*YFYEELSNMKRSMIDAASGGALGDMTPTEARNLIKKMASNSQQFSARNDAIVL---- 1078
Query: 178 SKKEAGIHEVS 188
G+HEV+
Sbjct: 1079----RGVHEVA 1099
>TC232867 similar to UP|Q9MA68 (Q9MA68) F2J6.13 protein, partial (3%)
Length = 1144
Score = 119 bits (297), Expect = 5e-27
Identities = 69/182 (37%), Positives = 101/182 (54%), Gaps = 3/182 (1%)
Frame = -3
Query: 2 NQFSGSPTEDPNLHISSFLRLSGTIK---ENQEAVRLHLFPFSLRDRASAWFHSLEVGSI 58
N F G P EDP H+++++ + TI+ +A+RL L FSL A W HS + S+
Sbjct: 788 NFFHGLPNEDPYAHLATYIEICNTIRLAGVPADAIRLSLLSFSLSGEAKRWLHSFKGNSL 609
Query: 59 TSWDDMRRAFLARFFPPSKTAKLRDQITRFNQKDGESLYEAWEHFKEMLRLCPHHGLEKW 118
SWD++ FL ++FP SKTA+ + I+ F+Q ESL EA E F+ +LR P HG +
Sbjct: 608 KSWDEVVEKFLKKYFPESKTAEGKAAISSFHQFPDESLSEALERFRGLLRKTPTHGFSEP 429
Query: 119 LIVHTFYNGLSDTTKMSVDAAAGGALMNKNYTEAYALIEDMAQNHYQWTNERAVTTPTPS 178
+ ++ F + L +K VDA+ GG + K EA LIE MA + +RA P+
Sbjct: 428 IQLNIFIDELRPESKQLVDASVGGKIKMKTPDEAMDLIESMAASDIAILRDRA---HIPT 258
Query: 179 KK 180
KK
Sbjct: 257 KK 252
>TC232398
Length = 1054
Score = 75.5 bits (184), Expect(2) = 1e-24
Identities = 37/92 (40%), Positives = 55/92 (59%)
Frame = +1
Query: 480 KRMGIGELKPTEMILKLADRSTIPLVGYIEDIPVKIEGIYIPTDFVVVDIEEDHDVPIIL 539
+R+G +++PT M L+LAD S G +EDI VK+ + DFV++DIEED ++ +IL
Sbjct: 172 RRIGNQKIEPTRMTLQLADHSITRSFGVVEDILVKVHQLIFLVDFVIMDIEEDAEIRLIL 351
Query: 540 GRPFLATAGAIVDVQSGRIVFQASDAMIGFEL 571
G PF+ TA +VD+ G + D F L
Sbjct: 352 GWPFMVTAKCVVDMGKGNLEMSVEDQKATFNL 447
Score = 56.6 bits (135), Expect(2) = 1e-24
Identities = 27/56 (48%), Positives = 36/56 (64%)
Frame = +2
Query: 392 IKIPFGEALSRMPLYTKFLKEIFSKKKAMDHKETIALTRESSAIVKKQPPKLRDPG 447
I IPFGE + +MPLY KFLK+I KK + ETI + A+++K PPK +D G
Sbjct: 8 ITIPFGERIQQMPLYKKFLKDILIKKGKYINSETIVVGEYCRALIQKLPPKFKDLG 175
>TC222094
Length = 984
Score = 63.5 bits (153), Expect(2) = 1e-21
Identities = 29/80 (36%), Positives = 47/80 (58%), Gaps = 3/80 (3%)
Frame = -2
Query: 2 NQFSGSPTEDPNLHISSFLRLSGTIK---ENQEAVRLHLFPFSLRDRASAWFHSLEVGSI 58
N F G PTEDP H+++++ + T+K ++A+ L LF FSL A W S + ++
Sbjct: 728 NLFYGLPTEDPYAHLATYIDICNTVKIVGVPEDAIHLDLFCFSLAGEARTWLRSFKGNNL 549
Query: 59 TSWDDMRRAFLARFFPPSKT 78
+W++ FL ++FP SKT
Sbjct: 548 RTWNEXXEKFLKKYFPESKT 489
Score = 58.2 bits (139), Expect(2) = 1e-21
Identities = 34/104 (32%), Positives = 57/104 (54%)
Frame = -3
Query: 84 QITRFNQKDGESLYEAWEHFKEMLRLCPHHGLEKWLIVHTFYNGLSDTTKMSVDAAAGGA 143
+I+ F+Q ESL EA + F +L P HG + + ++ F +G+ +K +DA+AGG
Sbjct: 472 EISSFHQHPHESLSEALDRFHGLLWKTPTHGFSEPVQLNIFIDGMQPHSKQLLDASAGGK 293
Query: 144 LMNKNYTEAYALIEDMAQNHYQWTNERAVTTPTPSKKEAGIHEV 187
+ K EA LIE+MA N Y ++ P+P ++ + E+
Sbjct: 292 IKLKTPEEAIELIENMAANDYVILRDQ---EPSPQEESTRLEEL 170
>BQ133845
Length = 389
Score = 96.7 bits (239), Expect = 3e-20
Identities = 52/125 (41%), Positives = 79/125 (62%), Gaps = 1/125 (0%)
Frame = +2
Query: 358 TLLDKNKALNPLRLTKLNLEAQFAKFLNILKRICIKIPFGEALSRMPLYTKFLKEIFSKK 417
TL++ + PL + + E AKFL+I K++ I +PF EAL +MPLY FLK++ +KK
Sbjct: 14 TLIECKEVPYPLVPS*KDKEQHLAKFLDIFKKLEITLPFEEALQQMPLYANFLKDMLTKK 193
Query: 418 KAMDHKETIALTRESSAIVKK-QPPKLRDPGSFVIPCVIGKETVDKALCDLGASVSLLPL 476
H + I + SA++++ PP DPG +PC IG+ V KAL DLGAS++L+PL
Sbjct: 194 NWYIHSDKIVVEGNCSAVIQRILPP*HTDPGFVTMPCSIGEVAVGKALIDLGASINLMPL 373
Query: 477 SLFKR 481
S+ ++
Sbjct: 374 SMCRQ 388
>CF922341
Length = 675
Score = 36.2 bits (82), Expect = 0.041
Identities = 18/64 (28%), Positives = 33/64 (51%)
Frame = +1
Query: 9 TEDPNLHISSFLRLSGTIKENQEAVRLHLFPFSLRDRASAWFHSLEVGSITSWDDMRRAF 68
T P H+ + + G +++E + +H F SL A W+ +LE + SW D+ AF
Sbjct: 448 TTCPKNHLKMYCQKMGAYAKDEELL-IHSFQESLTGVAVTWYTNLEPSRVHSWKDLMVAF 624
Query: 69 LARF 72
+ ++
Sbjct: 625 VRQY 636
>TC231541 similar to UP|Q8LCH9 (Q8LCH9) Thioredoxin-like protein, partial
(83%)
Length = 536
Score = 26.9 bits (58), Expect(2) = 0.30
Identities = 12/38 (31%), Positives = 21/38 (54%)
Frame = -3
Query: 540 GRPFLATAGAIVDVQSGRIVFQASDAMIGFELENVMKG 577
G P+L TAG + D+ R++ ++S + F L + G
Sbjct: 192 GAPYLMTAGVLEDLMCARMLVKSSSLVALFRLTRRLVG 79
Score = 25.0 bits (53), Expect(2) = 0.30
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = -2
Query: 487 LKPTEMILKLADRSTIPLVGYIEDIPVKIEGIYIP 521
++P ++ L STI L+ Y+ IPVK+EG +P
Sbjct: 412 VQPQTIMEPLFPTSTIHLI-YLFTIPVKLEGGSVP 311
>TC215407 weakly similar to GB|CAD29448.2|28804246|MMU440757 IL-1
receptor-associated kinase M {Mus musculus;} , partial
(6%)
Length = 1177
Score = 29.6 bits (65), Expect = 3.9
Identities = 30/123 (24%), Positives = 52/123 (41%), Gaps = 15/123 (12%)
Frame = +2
Query: 230 GYTNNQRVAQKSSLEILLENCMMNQNKQLQELKNQTGSLNDSLSKLNT-KVDSI----AT 284
G +NN + S+ +L C +NQ + L EL ++ L L+K+ KV I +
Sbjct: 401 GKSNNNYLPDDGSVCLLDRACQLNQTRNLMELIDE--RLGPDLNKMEVEKVVKIGLLCSN 574
Query: 285 HTKMLETQISQVAQQVAISS*TPGVFPGQTETNPKAHVNAI----------SLGGNKLED 334
+ L +S+V + + P V P + N A+ SL GN+ +
Sbjct: 575 ASPTLRPTMSEVVNMLEGHADIPDVIPEPSTYNDDLRFKALRNLHQYQSKQSLSGNQSQS 754
Query: 335 TIT 337
++T
Sbjct: 755 SMT 763
>TC217698 similar to UP|Q89GY6 (Q89GY6) Blr6209 protein, partial (5%)
Length = 865
Score = 29.6 bits (65), Expect = 3.9
Identities = 18/46 (39%), Positives = 21/46 (45%), Gaps = 5/46 (10%)
Frame = +2
Query: 199 QLNYAQYKQGMRPNQNF-----YKNPQGSYGQTAPPGYTNNQRVAQ 239
Q NY Q Q P QN+ PQ +YGQ A P Y Q+ Q
Sbjct: 44 QXNYGQASQNYPPQQNYGMTSQNYTPQQNYGQ-ASPNYPPQQKYGQ 178
>TC227422 similar to UP|P92973 (P92973) CCA1 (MYB-related transcription
factor (CCA1); supported by cDNA: gi:1777442) (MYB
transcription factor), partial (11%)
Length = 901
Score = 29.3 bits (64), Expect = 5.0
Identities = 18/68 (26%), Positives = 31/68 (45%), Gaps = 4/68 (5%)
Frame = -2
Query: 29 NQEAVRLHLFPFSLRDRASAWFHSLEVGSITSWDDM----RRAFLARFFPPSKTAKLRDQ 84
N++ +LH FPF L+ H S+ W + + F++ FF P+ +LR +
Sbjct: 360 NKQITKLHTFPFFLK-------H*NPQNSVQKWTSLATKQKHCFVSLFFYPTLLFQLRIE 202
Query: 85 ITRFNQKD 92
T +D
Sbjct: 201 DTLLRSRD 178
>TC213120 similar to UP|Q9JL44 (Q9JL44) ATP-binding cassette protein
(Fragment), partial (6%)
Length = 467
Score = 29.3 bits (64), Expect = 5.0
Identities = 28/110 (25%), Positives = 53/110 (47%), Gaps = 2/110 (1%)
Frame = +3
Query: 339 AKSVKGESVKLLGEKDAIKTLLDKNKALNPLRLTKLNLEAQFAK--FLNILKRICIKIPF 396
A+ + ESV+L + + ++ LDK+ +N + ++LN + ++ LN L+ +K
Sbjct: 153 ARYISNESVELKTDNNVVRFSLDKSDDINYGKKSQLNKRVKMSRKAKLNELRFYRLK--- 323
Query: 397 GEALSRMPLYTKFLKEIFSKKKAMDHKETIALTRESSAIVKKQPPKLRDP 446
A +M ++ + +KA KET + + V K PP+ DP
Sbjct: 324 --AKKKMISPNPEVRITYKLEKA-KRKETWLVEKLRKFDVPKSPPETFDP 464
>TC209544 similar to UP|Q8W040 (Q8W040) Dihydrofolate synthetase
/folylpolyglutamate synthetase, partial (25%)
Length = 986
Score = 28.9 bits (63), Expect = 6.6
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = +3
Query: 233 NNQRVAQKSSLEILLENCMMNQNKQL 258
+N QK S +ILL NCM +N QL
Sbjct: 321 HNGETVQKKSTQILLFNCMSERNPQL 398
>BG550748
Length = 411
Score = 28.9 bits (63), Expect = 6.6
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = +2
Query: 204 QYKQGMRPNQNFYKNPQGSYGQTAPPGY 231
++K+ + N NF+K+PQ + T P Y
Sbjct: 86 KHKENLNSNNNFWKSPQKTNAHTRQPNY 169
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.134 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,459,585
Number of Sequences: 63676
Number of extensions: 289917
Number of successful extensions: 1336
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1327
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1333
length of query: 577
length of database: 12,639,632
effective HSP length: 102
effective length of query: 475
effective length of database: 6,144,680
effective search space: 2918723000
effective search space used: 2918723000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)
Medicago: description of AC146583.2