BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146575.8 - phase: 0
(1078 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC232224 homologue to PIR|T51557|T51557 Exportin1 (XPO1) protein... 478 e-135
TC216810 similar to PIR|T51557|T51557 Exportin1 (XPO1) protein -... 442 e-124
TC208072 similar to PIR|T51557|T51557 Exportin1 (XPO1) protein -... 335 8e-92
BG156369 similar to PIR|T52638|T5 exportin 1 [validated] - Arabi... 253 3e-67
TC216809 similar to PIR|T51557|T51557 Exportin1 (XPO1) protein -... 91 2e-18
AW432610 80 6e-15
TC208095 similar to UP|Q8SBC8 (Q8SBC8) Transcription factor LIM,... 44 3e-04
CD391969 43 9e-04
AW306551 33 0.68
BE801431 31 3.4
TC212631 30 4.4
AW100971 similar to GP|27475612|em squalene monooxygenase 1 {Med... 30 7.5
TC205899 similar to UP|Q8GSM8 (Q8GSM8) Squalene monooxygenase 1 ... 30 7.5
TC225878 similar to GB|AAP88348.1|32815927|BT009714 At3g12630 {A... 29 9.8
AI736889 29 9.8
>TC232224 homologue to PIR|T51557|T51557 Exportin1 (XPO1) protein -
Arabidopsis thaliana (fragment) {Arabidopsis thaliana;}
, partial (23%)
Length = 762
Score = 478 bits (1229), Expect = e-135
Identities = 237/254 (93%), Positives = 245/254 (96%)
Frame = +1
Query: 536 ITKGKDNKAVIASNIMYVVGQYPRFLRAHWKFLKTVVNKLFEFMHETHPGVQDMACDTFL 595
ITKGKDNKAVIASNIMYVVGQYPRFLRAHWKFLKTVVNKLFEFMHETHPGVQDMACDTFL
Sbjct: 1 ITKGKDNKAVIASNIMYVVGQYPRFLRAHWKFLKTVVNKLFEFMHETHPGVQDMACDTFL 180
Query: 596 KIVQKCRRKFVITQVGENEPFVSELLSGLSTTIADLEPHQIHTFYESVGSMIQAESDSQK 655
KIVQKC+RKFVITQVGENEPFVSELL+GL TIADLEPHQIH+FYESVG MIQAESD+QK
Sbjct: 181 KIVQKCKRKFVITQVGENEPFVSELLTGLPITIADLEPHQIHSFYESVGHMIQAESDAQK 360
Query: 656 RDEYLQRLMVLPNQKWMEIIGQARQNADFLKDQDVIRTVLNVLQTNTSVASSLGTYFLPQ 715
RDEYLQRLM LPNQKWMEIIGQA QN DFLKDQDVIRTVLN++QTNTSVA+SLGTYFLPQ
Sbjct: 361 RDEYLQRLMELPNQKWMEIIGQAHQNVDFLKDQDVIRTVLNIMQTNTSVATSLGTYFLPQ 540
Query: 716 ITLIFLDMLNVYRMYSELISKSIAEGTPFTSRTSYVKLLRSVKRETLKLIETFLDKAEDQ 775
I+LIFLDMLNVYRMYSELISKSI EG PF SRTSYVKLLRSVKRETLKLIETFLDKAEDQ
Sbjct: 541 ISLIFLDMLNVYRMYSELISKSITEGGPFASRTSYVKLLRSVKRETLKLIETFLDKAEDQ 720
Query: 776 PQIGKQFVPPMMDP 789
PQIGKQFVPPMMDP
Sbjct: 721 PQIGKQFVPPMMDP 762
>TC216810 similar to PIR|T51557|T51557 Exportin1 (XPO1) protein - Arabidopsis
thaliana (fragment) {Arabidopsis thaliana;} , partial
(22%)
Length = 1237
Score = 442 bits (1136), Expect = e-124
Identities = 219/236 (92%), Positives = 223/236 (93%)
Frame = +3
Query: 843 NFEDYPEHRLKFFSLLRAIATHCFPALICLSSQQLKFVMDSIIWAFRHTERNIAETGLNL 902
NFEDYPEHRLKFFSLLRAIATHCFPALICLSSQQLK VMDSIIWAFRHTERNIAETGLNL
Sbjct: 3 NFEDYPEHRLKFFSLLRAIATHCFPALICLSSQQLKLVMDSIIWAFRHTERNIAETGLNL 182
Query: 903 LLEMLNKFQASEFCNQFYRTYFLTIEQEIFAVLTDTFHKPGFKLHVLVLQHLLCLAESGA 962
LLEML KFQ SEFCNQFYRTYFLTIEQEIFAVLTDTFHKPGFKLHVLVLQHL CL E+GA
Sbjct: 183 LLEMLKKFQGSEFCNQFYRTYFLTIEQEIFAVLTDTFHKPGFKLHVLVLQHLFCLLETGA 362
Query: 963 LTEPLWDAATNSYPYPSNGAFVREYTIKLLSTSFPNMTAAEVTQFVNGLFESTNDLSTFK 1022
LTEPLWD AT+ Y Y SN FVRE+TIKLLSTSFPNMT+AEVTQFVNGLFESTNDLSTFK
Sbjct: 363 LTEPLWDVATSQYSYSSNAVFVREFTIKLLSTSFPNMTSAEVTQFVNGLFESTNDLSTFK 542
Query: 1023 THIRDFLIQSKEFSAQDNKDLYAEEAAAQRERERQRMLSIPGLIAPVELQDEMVDS 1078
HIRDFLIQSKEFSAQDNKDLYAEEAAAQRERERQRMLSIPGLIAP ELQDEMVDS
Sbjct: 543 NHIRDFLIQSKEFSAQDNKDLYAEEAAAQRERERQRMLSIPGLIAPSELQDEMVDS 710
>TC208072 similar to PIR|T51557|T51557 Exportin1 (XPO1) protein - Arabidopsis
thaliana (fragment) {Arabidopsis thaliana;} , partial
(17%)
Length = 1106
Score = 335 bits (858), Expect = 8e-92
Identities = 165/179 (92%), Positives = 170/179 (94%)
Frame = +1
Query: 900 LNLLLEMLNKFQASEFCNQFYRTYFLTIEQEIFAVLTDTFHKPGFKLHVLVLQHLLCLAE 959
LNLLLEML KFQASEFCNQFYRTYFLTIEQEIFAVLTDTFHKPGFK HVLVLQHL CLAE
Sbjct: 1 LNLLLEMLKKFQASEFCNQFYRTYFLTIEQEIFAVLTDTFHKPGFKFHVLVLQHLFCLAE 180
Query: 960 SGALTEPLWDAATNSYPYPSNGAFVREYTIKLLSTSFPNMTAAEVTQFVNGLFESTNDLS 1019
+GALTEPLWDAATN YPYPSN AFVRE+TI LLSTSFPNMTA+EVTQFVNGL ESTNDL
Sbjct: 181 TGALTEPLWDAATNPYPYPSNAAFVREFTINLLSTSFPNMTASEVTQFVNGLLESTNDLP 360
Query: 1020 TFKTHIRDFLIQSKEFSAQDNKDLYAEEAAAQRERERQRMLSIPGLIAPVELQDEMVDS 1078
TFKTHIRDFL+QSKEFSAQDNKDLYAEEAAAQRERERQRMLSIPGL+AP ELQDEMVDS
Sbjct: 361 TFKTHIRDFLVQSKEFSAQDNKDLYAEEAAAQRERERQRMLSIPGLVAPSELQDEMVDS 537
>BG156369 similar to PIR|T52638|T5 exportin 1 [validated] - Arabidopsis
thaliana, partial (12%)
Length = 410
Score = 253 bits (646), Expect = 3e-67
Identities = 125/136 (91%), Positives = 132/136 (96%)
Frame = +2
Query: 595 LKIVQKCRRKFVITQVGENEPFVSELLSGLSTTIADLEPHQIHTFYESVGSMIQAESDSQ 654
LKIVQKC+RKFVITQVGENEPFVSELLSGL TIADLEPHQIHTFYESVG+MIQAESD+Q
Sbjct: 2 LKIVQKCKRKFVITQVGENEPFVSELLSGLPNTIADLEPHQIHTFYESVGNMIQAESDAQ 181
Query: 655 KRDEYLQRLMVLPNQKWMEIIGQARQNADFLKDQDVIRTVLNVLQTNTSVASSLGTYFLP 714
KRDEYLQ+LMVLPNQ+WMEIIG+A QNADFLKDQDVIR VLN+LQTNTSVASSLGTYFLP
Sbjct: 182 KRDEYLQKLMVLPNQRWMEIIGKAHQNADFLKDQDVIRNVLNILQTNTSVASSLGTYFLP 361
Query: 715 QITLIFLDMLNVYRMY 730
QITLIFLDMLNVYRMY
Sbjct: 362 QITLIFLDMLNVYRMY 409
>TC216809 similar to PIR|T51557|T51557 Exportin1 (XPO1) protein - Arabidopsis
thaliana (fragment) {Arabidopsis thaliana;} , partial
(5%)
Length = 689
Score = 91.3 bits (225), Expect = 2e-18
Identities = 47/55 (85%), Positives = 47/55 (85%)
Frame = +3
Query: 1024 HIRDFLIQSKEFSAQDNKDLYAEEAAAQRERERQRMLSIPGLIAPVELQDEMVDS 1078
HIRD IQSKEFSAQD KDLYAEEAA QRER RMLSIPGLIAP ELQDEMVDS
Sbjct: 3 HIRDXXIQSKEFSAQDXKDLYAEEAAXQRERXXXRMLSIPGLIAPSELQDEMVDS 167
>AW432610
Length = 411
Score = 79.7 bits (195), Expect = 6e-15
Identities = 39/41 (95%), Positives = 40/41 (97%)
Frame = +2
Query: 1038 QDNKDLYAEEAAAQRERERQRMLSIPGLIAPVELQDEMVDS 1078
QDNKDLYAEEAAAQRERERQRMLSIPGL+AP ELQDEMVDS
Sbjct: 77 QDNKDLYAEEAAAQRERERQRMLSIPGLVAPSELQDEMVDS 199
>TC208095 similar to UP|Q8SBC8 (Q8SBC8) Transcription factor LIM, partial
(23%)
Length = 1310
Score = 44.3 bits (103), Expect = 3e-04
Identities = 26/64 (40%), Positives = 31/64 (47%)
Frame = +1
Query: 587 QDMACDTFLKIVQKCRRKFVITQVGENEPFVSELLSGLSTTIADLEPHQIHTFYESVGSM 646
Q+MACDTFL +GL TIADLEPHQIH+FYES+ +
Sbjct: 778 QNMACDTFL--------------------------TGLPITIADLEPHQIHSFYESLSNY 879
Query: 647 IQAE 650
E
Sbjct: 880 CSFE 891
>CD391969
Length = 604
Score = 42.7 bits (99), Expect = 9e-04
Identities = 19/30 (63%), Positives = 23/30 (76%)
Frame = -3
Query: 621 LSGLSTTIADLEPHQIHTFYESVGSMIQAE 650
L+GL TIADLEPHQIH+FYES+ + E
Sbjct: 497 LTGLPITIADLEPHQIHSFYESLSNYCSFE 408
>AW306551
Length = 352
Score = 33.1 bits (74), Expect = 0.68
Identities = 18/50 (36%), Positives = 28/50 (56%), Gaps = 2/50 (4%)
Frame = +1
Query: 216 TLSTLHAFLSWIPLGYIFESPLLETLLKFFTMQAY--RNLTLQCLTEVAS 263
T + HA LSW+P I S L L+ FT++ + ++ +CL EVA+
Sbjct: 1 TCAAAHASLSWLPSNIICTSTFLVLSLENFTLKFFYINSMVRRCLKEVAN 150
>BE801431
Length = 421
Score = 30.8 bits (68), Expect = 3.4
Identities = 18/47 (38%), Positives = 27/47 (57%)
Frame = +2
Query: 220 LHAFLSWIPLGYIFESPLLETLLKFFTMQAYRNLTLQCLTEVASLQF 266
+H FLS + + LL + K+F ++ +RNL L+ T VASL F
Sbjct: 164 VHLFLSTVTPAHSLLDLLLNSSFKYFDIRCFRNLVLKFNT-VASLNF 301
>TC212631
Length = 622
Score = 30.4 bits (67), Expect = 4.4
Identities = 19/64 (29%), Positives = 27/64 (41%)
Frame = -1
Query: 471 SHLDHDDTEKQMLGKLSKQLSGKDWTWNNLNTLCWAIGSISGSMGEEQENRFLVMVIRDL 530
S LD D+ G L G D CW +GSI+ M E N F+V ++
Sbjct: 355 SGLDRFDSISSGSGFSCGVLKGSD------RVRCWGVGSIARKMESEFRNMFMVSIVDGE 194
Query: 531 LNLC 534
++C
Sbjct: 193 SHVC 182
>AW100971 similar to GP|27475612|em squalene monooxygenase 1 {Medicago
truncatula}, partial (27%)
Length = 439
Score = 29.6 bits (65), Expect = 7.5
Identities = 15/46 (32%), Positives = 26/46 (55%)
Frame = +2
Query: 180 LKIKELKQSLNSEFQLIHELCLFVLSVSQRTELIRATLSTLHAFLS 225
LK+ K+ + +QL+H L LFV++VSQ ++ L + +S
Sbjct: 41 LKVCTTKRRIVKNYQLVHPLPLFVMAVSQTCAVLFVILR*MFPIIS 178
>TC205899 similar to UP|Q8GSM8 (Q8GSM8) Squalene monooxygenase 1 , partial
(67%)
Length = 1133
Score = 29.6 bits (65), Expect = 7.5
Identities = 15/46 (32%), Positives = 26/46 (55%)
Frame = +2
Query: 180 LKIKELKQSLNSEFQLIHELCLFVLSVSQRTELIRATLSTLHAFLS 225
LK+ K+ + +QL+H L LFV++VSQ ++ L + +S
Sbjct: 65 LKVCTTKRRIVKNYQLVHPLPLFVMAVSQTCAVLFVILR*MFPIIS 202
>TC225878 similar to GB|AAP88348.1|32815927|BT009714 At3g12630 {Arabidopsis
thaliana;} , partial (66%)
Length = 995
Score = 29.3 bits (64), Expect = 9.8
Identities = 15/38 (39%), Positives = 21/38 (54%)
Frame = -1
Query: 1021 FKTHIRDFLIQSKEFSAQDNKDLYAEEAAAQRERERQR 1058
F +H DFL KE ++ +DL + RERER+R
Sbjct: 167 FLSHGLDFLFFHKERKKENYEDLKGFDEIVMRERERER 54
>AI736889
Length = 364
Score = 29.3 bits (64), Expect = 9.8
Identities = 19/64 (29%), Positives = 32/64 (49%)
Frame = +2
Query: 188 SLNSEFQLIHELCLFVLSVSQRTELIRATLSTLHAFLSWIPLGYIFESPLLETLLKFFTM 247
SL + H LCL + + + T LH++L W+ + LL+TLL+ F++
Sbjct: 8 SLENCVSASHVLCLRSSCWTHTSTTLLITRPHLHSYLLWV-------AGLLDTLLQPFSL 166
Query: 248 QAYR 251
Q +R
Sbjct: 167 QLWR 178
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.136 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,200,846
Number of Sequences: 63676
Number of extensions: 555745
Number of successful extensions: 2972
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 2957
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2970
length of query: 1078
length of database: 12,639,632
effective HSP length: 107
effective length of query: 971
effective length of database: 5,826,300
effective search space: 5657337300
effective search space used: 5657337300
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 64 (29.3 bits)
Medicago: description of AC146575.8