BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146570.6 + phase: 0
(101 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC221966 similar to UP|Q9M9A2 (Q9M9A2) F27J15.21 (At1g49000), pa... 63 2e-11
TC219290 similar to UP|Q9M9A2 (Q9M9A2) F27J15.21 (At1g49000), pa... 60 2e-10
BG509584 similar to GP|7770334|gb|A F27J15.21 {Arabidopsis thali... 48 7e-07
TC212945 weakly similar to UP|Q9M9H2 (Q9M9H2) F14O23.12, partial... 46 3e-06
TC210888 similar to UP|Q41051 (Q41051) Proline- and leucine-rich... 35 0.006
TC204050 homologue to UP|RR18_ARATH (P56807) Chloroplast 30S rib... 29 0.32
TC214255 weakly similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, part... 28 0.93
TC209998 27 1.2
TC211724 similar to UP|Q8LLE5 (Q8LLE5) Coiled-coil protein (Frag... 27 2.1
TC205781 similar to UP|Q93WR8 (Q93WR8) MEK map kinase kinsae, pa... 27 2.1
TC224715 homologue to UP|Q39882 (Q39882) Nodulin-26, partial (98%) 27 2.1
TC209906 26 2.7
TC211950 similar to UP|PNK1_ARATH (Q8L5Y9) Probable pantothenate... 26 2.7
TC211013 similar to UP|Q27529 (Q27529) DNA topoisomerase I (Dna ... 26 3.5
BU080965 26 3.5
TC205782 similar to UP|Q93WR8 (Q93WR8) MEK map kinase kinsae, pa... 25 4.6
TC207677 25 4.6
TC207604 similar to UP|P93378 (P93378) Tumor-related protein, pa... 25 4.6
TC221831 UP|Q8LJS1 (Q8LJS1) No apical meristem-like protein, com... 25 4.6
TC225216 homologue to UP|Q6PW04 (Q6PW04) Membrane-anchored endo-... 25 4.6
>TC221966 similar to UP|Q9M9A2 (Q9M9A2) F27J15.21 (At1g49000), partial (29%)
Length = 773
Score = 63.2 bits (152), Expect = 2e-11
Identities = 34/70 (48%), Positives = 47/70 (66%), Gaps = 7/70 (10%)
Frame = +3
Query: 34 KKIYKAIHCKIL---KMKKQEDD---KCLWKKTILMGEKCQPLEFPGAIFYDSEGNQLSE 87
KK+ I K L K K+ E+D +W+K ILMG+KC+PL+F G I+YDS G Q++E
Sbjct: 105 KKLLSNISSKTLPFRKKKRGEEDWGKGGVWQKAILMGDKCEPLDFSGVIYYDSNGKQVNE 284
Query: 88 PP-RTPRSSP 96
P R+PR+SP
Sbjct: 285 MPLRSPRASP 314
>TC219290 similar to UP|Q9M9A2 (Q9M9A2) F27J15.21 (At1g49000), partial (32%)
Length = 685
Score = 60.1 bits (144), Expect = 2e-10
Identities = 39/99 (39%), Positives = 52/99 (52%), Gaps = 18/99 (18%)
Frame = +2
Query: 16 SLKTKKLEELSTSSKNIIKKIYKAIHCKILKMKKQEDDKCL-----------------WK 58
++K K +EL SS+ KK+ I K L K+ + K L W+
Sbjct: 164 AVKASKEDELKKSSRP--KKLLSNISSKALFGHKKNNKKQLRSVEETQQAQGWGNGGVWQ 337
Query: 59 KTILMGEKCQPLEFPGAIFYDSEGNQLSE-PPRTPRSSP 96
K ILMG KC+PL F G I+YD G QLS+ P R+PR+SP
Sbjct: 338 KEILMGGKCEPLNFSGVIYYDGNGKQLSQIPLRSPRASP 454
>BG509584 similar to GP|7770334|gb|A F27J15.21 {Arabidopsis thaliana},
partial (23%)
Length = 327
Score = 48.1 bits (113), Expect = 7e-07
Identities = 20/34 (58%), Positives = 28/34 (81%), Gaps = 1/34 (2%)
Frame = +2
Query: 64 GEKCQPLEFPGAIFYDSEGNQLSEPP-RTPRSSP 96
G+KC+PL+F G I+YDS G Q++E P R+PR+SP
Sbjct: 2 GDKCEPLDFSGVIYYDSNGKQVNEMPLRSPRASP 103
>TC212945 weakly similar to UP|Q9M9H2 (Q9M9H2) F14O23.12, partial (15%)
Length = 625
Score = 46.2 bits (108), Expect = 3e-06
Identities = 29/84 (34%), Positives = 41/84 (48%), Gaps = 22/84 (26%)
Frame = +2
Query: 20 KKLEELSTSSKN-----IIKKIYKAIHCKILKMKKQ-----------------EDDKCLW 57
K+ E+ +T S N +I K+ I+ + L M K +D++ LW
Sbjct: 164 KEAEKATTRSSNSKEHKLISKLNSNINNRALSMVKMISWRKVREGEVEGDYSDQDEEALW 343
Query: 58 KKTILMGEKCQPLEFPGAIFYDSE 81
+K ILMGEKC+PL F G DSE
Sbjct: 344 RKNILMGEKCRPLNFSGKFENDSE 415
>TC210888 similar to UP|Q41051 (Q41051) Proline- and leucine-rich protein,
partial (12%)
Length = 771
Score = 35.0 bits (79), Expect = 0.006
Identities = 14/32 (43%), Positives = 20/32 (61%)
Frame = +3
Query: 56 LWKKTILMGEKCQPLEFPGAIFYDSEGNQLSE 87
+W++ IL GEKC+ F G I YD +G L +
Sbjct: 501 VWQRPILRGEKCELPRFSGLILYDEKGRLLRD 596
>TC204050 homologue to UP|RR18_ARATH (P56807) Chloroplast 30S ribosomal
protein S18, partial (95%)
Length = 1669
Score = 29.3 bits (64), Expect = 0.32
Identities = 18/54 (33%), Positives = 31/54 (57%), Gaps = 3/54 (5%)
Frame = -1
Query: 32 IIKKIYKAIH---CKILKMKKQEDDKCLWKKTILMGEKCQPLEFPGAIFYDSEG 82
I+ IY +H C+ L+ KK+E +C+WKKTI + ++ + P + +Y G
Sbjct: 418 ILNWIYNLLHPFPCQ*LE*KKREY-QCIWKKTIDLYQETRQSPEP*STYYWCHG 260
>TC214255 weakly similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (19%)
Length = 3445
Score = 27.7 bits (60), Expect = 0.93
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +2
Query: 8 KAIISYIISLKTKKLEELSTSSKNIIKKIYK 38
++I S SLK KK+++L+ K +IKKI K
Sbjct: 665 QSINSVNCSLKKKKIKDLNAKRKAVIKKIEK 757
>TC209998
Length = 834
Score = 27.3 bits (59), Expect = 1.2
Identities = 13/30 (43%), Positives = 18/30 (59%), Gaps = 3/30 (10%)
Frame = -2
Query: 64 GEKCQPL---EFPGAIFYDSEGNQLSEPPR 90
G C+PL P ++ + S+G LSEPPR
Sbjct: 287 GNICRPLLQLVLPSSLHHGSKGKVLSEPPR 198
>TC211724 similar to UP|Q8LLE5 (Q8LLE5) Coiled-coil protein (Fragment),
partial (12%)
Length = 1086
Score = 26.6 bits (57), Expect = 2.1
Identities = 19/62 (30%), Positives = 33/62 (52%), Gaps = 3/62 (4%)
Frame = +3
Query: 5 KINKAIISYIISLKTKKLEELSTSSKNIIKKIYK--AIHCK-ILKMKKQEDDKCLWKKTI 61
K KAI+ + L ++EEL + +++ +KI K A+ K +K K ED+ K +
Sbjct: 450 KAKKAIVESTLKLTQTEVEELISQIRSLEEKIQKERALSAKNSIKWGKLEDELSKMKHKV 629
Query: 62 LM 63
L+
Sbjct: 630 LV 635
>TC205781 similar to UP|Q93WR8 (Q93WR8) MEK map kinase kinsae, partial (47%)
Length = 935
Score = 26.6 bits (57), Expect = 2.1
Identities = 10/21 (47%), Positives = 15/21 (70%)
Frame = -1
Query: 79 DSEGNQLSEPPRTPRSSPSSS 99
D + +S+PPR RSSP+S+
Sbjct: 152 DPSSDSVSDPPRXQRSSPASA 90
>TC224715 homologue to UP|Q39882 (Q39882) Nodulin-26, partial (98%)
Length = 1270
Score = 26.6 bits (57), Expect = 2.1
Identities = 16/49 (32%), Positives = 20/49 (40%), Gaps = 5/49 (10%)
Frame = +2
Query: 56 LWKKTILMGEKCQPLEFPGAIFYDSEGNQL-----SEPPRTPRSSPSSS 99
LW + C P + P + S L S PP +P SPSSS
Sbjct: 569 LWLSSSQPSSSCSPAQAPASPTTSSPTTALPPLPASSPPPSPMHSPSSS 715
>TC209906
Length = 538
Score = 26.2 bits (56), Expect = 2.7
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = -3
Query: 9 AIISYIISLKTKKLEELSTSSKNIIKKIYKAIHCKILK 46
AI+ ++ L+ K ++ S KN+ K + IHC LK
Sbjct: 308 AILCCLVHLEQAKELRINASYKNVRGKFGRDIHCNDLK 195
>TC211950 similar to UP|PNK1_ARATH (Q8L5Y9) Probable pantothenate kinase 1
(Pantothenic acid kinase 1) , partial (10%)
Length = 779
Score = 26.2 bits (56), Expect = 2.7
Identities = 14/53 (26%), Positives = 26/53 (48%)
Frame = +3
Query: 30 KNIIKKIYKAIHCKILKMKKQEDDKCLWKKTILMGEKCQPLEFPGAIFYDSEG 82
K I +IY+ H K+ + + +D ++ + G+K PL +F D+ G
Sbjct: 543 KGTILEIYRISHNKMQRPWRVDDFHAFKQRMLGTGDKKPPLHRRALLFVDNAG 701
>TC211013 similar to UP|Q27529 (Q27529) DNA topoisomerase I (Dna
topoisomerase) , partial (3%)
Length = 410
Score = 25.8 bits (55), Expect = 3.5
Identities = 12/38 (31%), Positives = 23/38 (59%)
Frame = +2
Query: 13 YIISLKTKKLEELSTSSKNIIKKIYKAIHCKILKMKKQ 50
Y+I +K KK ++ + ++ K+ A+H ILK ++Q
Sbjct: 8 YVIKIKLKKNKKSNNNNCFAKPKLQGAVHKMILKQREQ 121
>BU080965
Length = 422
Score = 25.8 bits (55), Expect = 3.5
Identities = 9/27 (33%), Positives = 17/27 (62%)
Frame = -3
Query: 29 SKNIIKKIYKAIHCKILKMKKQEDDKC 55
++NI+ K+ + + K+LK K +D C
Sbjct: 165 NRNIVNKVCQVMQAKMLK*KASSEDHC 85
>TC205782 similar to UP|Q93WR8 (Q93WR8) MEK map kinase kinsae, partial (91%)
Length = 1377
Score = 25.4 bits (54), Expect = 4.6
Identities = 10/21 (47%), Positives = 15/21 (70%)
Frame = -2
Query: 79 DSEGNQLSEPPRTPRSSPSSS 99
D + S+PPR+ RSSP+S+
Sbjct: 725 DPSSDSESDPPRSQRSSPASA 663
>TC207677
Length = 1373
Score = 25.4 bits (54), Expect = 4.6
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = +2
Query: 78 YDSEGNQLSEPPRTPRSSPSS 98
Y+S PPR+P SSPSS
Sbjct: 71 YESSPPWAESPPRSPPSSPSS 133
>TC207604 similar to UP|P93378 (P93378) Tumor-related protein, partial (44%)
Length = 816
Score = 25.4 bits (54), Expect = 4.6
Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 4/57 (7%)
Frame = +1
Query: 46 KMKKQEDD-KCLWKKTILMGEKCQPLEFPGAIFYDSEGNQ---LSEPPRTPRSSPSS 98
K+++ +DD K ++ T+ C+PL +F DS GNQ L++ P R PS+
Sbjct: 487 KIEEYDDDYKLVFCPTVC--NFCKPLCRNVGVFRDSNGNQRVALTDEPYKVRFQPSA 651
>TC221831 UP|Q8LJS1 (Q8LJS1) No apical meristem-like protein, complete
Length = 1216
Score = 25.4 bits (54), Expect = 4.6
Identities = 10/24 (41%), Positives = 15/24 (61%)
Frame = +2
Query: 77 FYDSEGNQLSEPPRTPRSSPSSSF 100
FY+++G QL PP P S S+ +
Sbjct: 896 FYNTQGVQLQAPPTLPLPSSSNHY 967
>TC225216 homologue to UP|Q6PW04 (Q6PW04) Membrane-anchored
endo-1,4-beta-glucanase , complete
Length = 2204
Score = 25.4 bits (54), Expect = 4.6
Identities = 11/16 (68%), Positives = 12/16 (74%)
Frame = +3
Query: 84 QLSEPPRTPRSSPSSS 99
Q S PP +P SSPSSS
Sbjct: 225 QSSSPPSSPASSPSSS 272
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.315 0.132 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,642,056
Number of Sequences: 63676
Number of extensions: 59779
Number of successful extensions: 376
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 374
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 376
length of query: 101
length of database: 12,639,632
effective HSP length: 77
effective length of query: 24
effective length of database: 7,736,580
effective search space: 185677920
effective search space used: 185677920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 51 (24.3 bits)
Medicago: description of AC146570.6