BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146567.12 + phase: 0 /pseudo
(307 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC208820 similar to UP|Q6NL07 (Q6NL07) At1g13820, partial (83%) 341 e-111
BU546722 69 3e-12
TC233337 similar to GB|AAO63446.1|28951045|BT005382 At1g64670 {A... 59 3e-09
TC207684 weakly similar to UP|Q8YXK7 (Q8YXK7) All1205 protein, p... 44 9e-05
TC226960 similar to UP|Q9ZP87 (Q9ZP87) Epoxide hydrolase, partia... 43 2e-04
TC210122 similar to GB|AAP42732.1|30984538|BT008719 At4g36530 {A... 42 3e-04
BI973866 weakly similar to GP|1354849|gb| epoxide hydrolase {Nic... 42 5e-04
TC231214 similar to UP|Q9SD45 (Q9SD45) Epoxide hydrolase-like pr... 41 6e-04
TC203901 weakly similar to GB|AAP42732.1|30984538|BT008719 At4g3... 41 8e-04
TC228751 similar to GB|AAO63388.1|28950929|BT005324 At5g19850 {A... 40 0.002
AW278730 37 0.009
TC204880 similar to UP|Q93ZN4 (Q93ZN4) AT4g12830/T20K18_180, par... 37 0.015
AI900813 weakly similar to PIR|T45731|T457 epoxide hydrolase-lik... 37 0.015
BI424408 similar to PIR|T45731|T45 epoxide hydrolase-like protei... 35 0.043
BE210363 35 0.056
TC232364 weakly similar to GB|AAL11601.1|15983466|AF424607 At1g3... 34 0.073
TC214636 similar to UP|Q39856 (Q39856) Epoxide hydrolase, partia... 34 0.073
TC216299 similar to UP|Q9SSQ3 (Q9SSQ3) F6D8.27 protein, partial ... 34 0.095
BQ453305 33 0.12
TC218968 similar to GB|AAD39677.1|5103847|F9L1 Contains PF|00561... 33 0.12
>TC208820 similar to UP|Q6NL07 (Q6NL07) At1g13820, partial (83%)
Length = 1422
Score = 341 bits (874), Expect(2) = e-111
Identities = 166/205 (80%), Positives = 179/205 (86%)
Frame = +2
Query: 26 FLPKDVNRIKDPFARTLATRIQRLPVSVKFSENPIMSSCVKPLVQNKENPIVLLHGFDSS 85
F P++ K FAR A RIQRLPV V+FSENPIMSSCVKPLVQ KE P+VLLHGFDSS
Sbjct: 305 FSPENFTPFKTLFARKFALRIQRLPVPVRFSENPIMSSCVKPLVQTKETPVVLLHGFDSS 484
Query: 86 CLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSKREHFYQFWKSYIKKPMIL 145
CLEWRY PLLEE+GIETWAIDILGWGFSDLEKLP CDVVSKR+HFYQFWKSYI++PMIL
Sbjct: 485 CLEWRYVLPLLEESGIETWAIDILGWGFSDLEKLPPCDVVSKRDHFYQFWKSYIRRPMIL 664
Query: 146 VGPSLGSAVAIDFAINYPEAVEKLVLIDASVYTEGTGNLATLPRAAAYAGVYLLKSVPLR 205
VGPSLGSAVAIDFA+NYPEAV+KLVLI ASVY EGTG LATLPRA AYAGV LLKS+PLR
Sbjct: 665 VGPSLGSAVAIDFAVNYPEAVKKLVLIGASVYAEGTGKLATLPRAVAYAGVNLLKSLPLR 844
Query: 206 VYANYLSFTNISFSTSLDWTNATML 230
+YA YL+FT ISFSTSLDWTN L
Sbjct: 845 LYATYLTFTKISFSTSLDWTNVGRL 919
Score = 79.3 bits (194), Expect(2) = e-111
Identities = 47/80 (58%), Positives = 57/80 (70%)
Frame = +3
Query: 228 TMLLRR*KW*SRKHS*FGGRTTA*SAISLLCNCTVNYLMQLYAKYLIVVTYLMLKDQILL 287
TM L *+ *+RKH *+G + TA SAISL C+ TVNYLMQLYAKY IV +L+ KDQILL
Sbjct: 978 TMFLH**ER*NRKH**YGVKMTASSAISLQCDSTVNYLMQLYAKYQIVDIFLIWKDQILL 1157
Query: 288 *NSLRNLFRRIPRQ*INMFH 307
N L NL + R *+N+ H
Sbjct: 1158 SN*LLNLCKERARH*VNVLH 1217
Score = 38.9 bits (89), Expect = 0.003
Identities = 15/28 (53%), Positives = 22/28 (78%)
Frame = +1
Query: 15 VAETDADSFPSFLPKDVNRIKDPFARTL 42
VA DAD FPSFLP++++ I+DPF + +
Sbjct: 271 VASADADGFPSFLPRELHTIQDPFCQKI 354
>BU546722
Length = 611
Score = 68.9 bits (167), Expect = 3e-12
Identities = 38/65 (58%), Positives = 48/65 (73%)
Frame = -2
Query: 243 *FGGRTTA*SAISLLCNCTVNYLMQLYAKYLIVVTYLMLKDQILL*NSLRNLFRRIPRQ* 302
*+G + TA SAISL C+ TV+Y+MQLYAKYLIV +L+LKDQILL N L NL + R
Sbjct: 328 *YGVKMTASSAISLQCDSTVSYVMQLYAKYLIVDIFLILKDQILLSN*LLNLCKERVRHS 149
Query: 303 INMFH 307
+N+ H
Sbjct: 148 VNVLH 134
>TC233337 similar to GB|AAO63446.1|28951045|BT005382 At1g64670 {Arabidopsis
thaliana;} , partial (42%)
Length = 658
Score = 58.9 bits (141), Expect = 3e-09
Identities = 44/154 (28%), Positives = 75/154 (48%), Gaps = 6/154 (3%)
Frame = +2
Query: 60 IMSSCVKPLVQNKENPIVLLHGFDSSCLEWRYT-YPLLEEAGIETWAIDILGWGFSDLEK 118
+ S C P +V +HGF SS L W T +P L ++ +A+D+LG+G S
Sbjct: 62 LKSQC--PTTDEAGEDVVFIHGFISSSLFWSETVFPNLSKSCYRLFAVDLLGFGRS---P 226
Query: 119 LPSCDVVSKREHFYQFWKSYIK----KPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDA 174
P+ + + REH +S ++ K +V SLG +A+ A+ +P++V+ L L+
Sbjct: 227 KPNSSLYTLREHLEMIERSVLEAHKVKSFHIVAHSLGCILALALAVKHPQSVKSLTLLAP 406
Query: 175 SVYTEGTGNLATLPRAAAYAGVYLLKSV-PLRVY 207
Y +P+ A Y+++ V P RV+
Sbjct: 407 PFY--------PVPKGETQATQYVMRKVAPRRVW 484
>TC207684 weakly similar to UP|Q8YXK7 (Q8YXK7) All1205 protein, partial (25%)
Length = 1322
Score = 43.9 bits (102), Expect = 9e-05
Identities = 31/108 (28%), Positives = 56/108 (51%), Gaps = 1/108 (0%)
Frame = +1
Query: 69 VQNKENPIVLL-HGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSK 127
V ++ P +LL HGF + +R L E+G WAI ILG+G S+ + +++
Sbjct: 130 VAGEDGPAILLVHGFGAFWEHYRDNIHGLAESGNRVWAITILGFGKSEKPNVVYTELL-W 306
Query: 128 REHFYQFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDAS 175
E F + +P+ LVG S+G + A + + ++ +VLI+++
Sbjct: 307 AELLRDFIVDIVGEPVHLVGNSIGGYLVAIVARVWSDLIKSIVLINSA 450
>TC226960 similar to UP|Q9ZP87 (Q9ZP87) Epoxide hydrolase, partial (72%)
Length = 1201
Score = 42.7 bits (99), Expect = 2e-04
Identities = 31/103 (30%), Positives = 45/103 (43%), Gaps = 4/103 (3%)
Frame = +1
Query: 74 NPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSD----LEKLPSCDVVSKRE 129
N +V LHGF WR+ L AG + D G+G SD +K D++S
Sbjct: 130 NAVVFLHGFPEIWYSWRHQMIALAGAGFRAVSFDYRGYGLSDPPPEPDKTSWSDLLSDLL 309
Query: 130 HFYQFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLI 172
H + + LVG G+ A F+I +PE V +V +
Sbjct: 310 HILD---ALALSKVFLVGKDFGARPAYLFSILHPERVLGVVTL 429
>TC210122 similar to GB|AAP42732.1|30984538|BT008719 At4g36530 {Arabidopsis
thaliana;} , partial (21%)
Length = 534
Score = 42.4 bits (98), Expect = 3e-04
Identities = 19/44 (43%), Positives = 30/44 (68%)
Frame = +1
Query: 68 LVQNKENPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGW 111
+VQ + +PIVL+HGF +S WRY P L + + +A+D+LG+
Sbjct: 406 VVQGEGSPIVLIHGFGASAFHWRYNIPELAKKH-KVYALDLLGF 534
>BI973866 weakly similar to GP|1354849|gb| epoxide hydrolase {Nicotiana
tabacum}, partial (33%)
Length = 420
Score = 41.6 bits (96), Expect = 5e-04
Identities = 30/103 (29%), Positives = 45/103 (43%), Gaps = 4/103 (3%)
Frame = +1
Query: 74 NPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDL----EKLPSCDVVSKRE 129
N +V LHGF WR+ L +AG + D G+G SD K D+++
Sbjct: 88 NAVVFLHGFPEIWYSWRHQMIALADAGFRAVSFDYRGYGLSDPPPPGNKATWFDLLNDLL 267
Query: 130 HFYQFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLI 172
H + + LVG G+ A F+I +PE V +V +
Sbjct: 268 HILD---ALALSKVFLVGKDFGARPAHFFSILHPERVLGVVTL 387
>TC231214 similar to UP|Q9SD45 (Q9SD45) Epoxide hydrolase-like protein
(AT3g51000/F24M12_40), partial (73%)
Length = 1104
Score = 41.2 bits (95), Expect = 6e-04
Identities = 29/110 (26%), Positives = 45/110 (40%), Gaps = 8/110 (7%)
Frame = +2
Query: 76 IVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSC--------DVVSK 127
++LLHGF + WR+ L G A D+ G+G SD PS D++
Sbjct: 146 VLLLHGFPETWYAWRHQINFLAHHGYHVVAPDLRGYGDSDSPIDPSSYTIHHLVGDIIGF 325
Query: 128 REHFYQFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDASVY 177
+HF Q +VG G+ + ++ PE V+ V + Y
Sbjct: 326 LDHFGQ-------HQAFIVGSDWGAVIGWHLSLFRPERVKGFVALSVPYY 454
>TC203901 weakly similar to GB|AAP42732.1|30984538|BT008719 At4g36530
{Arabidopsis thaliana;} , partial (20%)
Length = 1851
Score = 40.8 bits (94), Expect = 8e-04
Identities = 31/105 (29%), Positives = 48/105 (45%), Gaps = 11/105 (10%)
Frame = +1
Query: 108 ILGWGFSDLEKLPSCDVVSKREHFYQFWKSYIK--------KPMILVGPSLGSAVAIDFA 159
+ WGF D K +K + W+ ++ +P+ LVG SLG VA+ FA
Sbjct: 724 VSSWGFGDETK----PWATKLVYSVDLWQDQVRCFI*EVIGEPVYLVGNSLGGLVALYFA 891
Query: 160 INYPEAVEKLVLIDASVYTEGTGNLATLPRAA---AYAGVYLLKS 201
N P V+ + L++A+ + N PR A +AG + L S
Sbjct: 892 ANNPHLVKGVALLNATPFWGFLPNPIKSPRLAKIFPWAGTFPLPS 1026
>TC228751 similar to GB|AAO63388.1|28950929|BT005324 At5g19850 {Arabidopsis
thaliana;} , partial (81%)
Length = 1381
Score = 39.7 bits (91), Expect = 0.002
Identities = 25/109 (22%), Positives = 53/109 (47%), Gaps = 8/109 (7%)
Frame = +2
Query: 76 IVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSKREHFYQFW 135
+VL+HGF ++ WR +L ++ ++ID++G+G+SD K + + ++ W
Sbjct: 311 LVLVHGFGANSDHWRNNISVLAQSH-RVYSIDLIGYGYSD--KPNPRQIGDHSFYTFETW 481
Query: 136 KS--------YIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDASV 176
+ IK + S+G V + A+ P + ++L++ S+
Sbjct: 482 ATQLNEFCLDVIKDEAFFICNSIGGVVGLQAAVLAPHICQGIILLNISL 628
>AW278730
Length = 425
Score = 37.4 bits (85), Expect = 0.009
Identities = 26/88 (29%), Positives = 43/88 (48%), Gaps = 5/88 (5%)
Frame = +1
Query: 105 AIDILGWGFSDLEKLPSCDVVSKRE-----HFYQFWKSYIKKPMILVGPSLGSAVAIDFA 159
AID LGWG S +C + E F ++ K+ IL+G S G VA +A
Sbjct: 130 AIDQLGWGGSSRPDF-TCRSTEETEAWFVDSFEEWRKAKNLSNFILLGHSFGGYVASKYA 306
Query: 160 INYPEAVEKLVLIDASVYTEGTGNLATL 187
+ +PE V+ L+L+ + ++ + + L
Sbjct: 307 LKHPEHVKHLILVGPAGFSSESERITKL 390
>TC204880 similar to UP|Q93ZN4 (Q93ZN4) AT4g12830/T20K18_180, partial (83%)
Length = 1213
Score = 36.6 bits (83), Expect = 0.015
Identities = 39/142 (27%), Positives = 62/142 (43%), Gaps = 9/142 (6%)
Frame = +1
Query: 73 ENP-IVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSKREHF 131
+NP ++L+HGF S +R P+L + A D LG+GFSD + P + +
Sbjct: 205 DNPSVILIHGFPSQAYSYRKVLPVLSK-DYHVIAFDWLGFGFSD-KPQPRYGFDYTLDEY 378
Query: 132 YQFWKSYIKK----PMILVGPSLGSAVAIDFAINYPEAVEKLVLIDASVYTEGTGNLATL 187
+S I + + LV S V +A + E V L+L++ + T A L
Sbjct: 379 VSSLESLIDELAATKVSLVVQGYFSPVVAKYASIHQEKVRNLILLNPPL----TAQHAKL 546
Query: 188 PRAAAYAGVYLLKSV----PLR 205
P + +LL + PLR
Sbjct: 547 PPTLSIFSNFLLGEIFSQDPLR 612
>AI900813 weakly similar to PIR|T45731|T457 epoxide hydrolase-like protein -
Arabidopsis thaliana, partial (28%)
Length = 406
Score = 36.6 bits (83), Expect = 0.015
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +2
Query: 76 IVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSD 115
+++LHGF +C WR+ L G A D++G+G SD
Sbjct: 71 VLMLHGFPQTCDAWRHQINFLAHHGYHVVAPDLIGYGDSD 190
>BI424408 similar to PIR|T45731|T45 epoxide hydrolase-like protein -
Arabidopsis thaliana, partial (33%)
Length = 421
Score = 35.0 bits (79), Expect = 0.043
Identities = 21/66 (31%), Positives = 30/66 (44%), Gaps = 8/66 (12%)
Frame = +2
Query: 76 IVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSC--------DVVSK 127
++LLHGF + WR+ L G A D+ G+G SD PS D++
Sbjct: 146 VLLLHGFPETWYAWRHQINFLAHHGYHVVAPDLRGYGDSDSPIDPSSYTIHHLVGDIIGF 325
Query: 128 REHFYQ 133
+HF Q
Sbjct: 326 LDHFGQ 343
>BE210363
Length = 400
Score = 34.7 bits (78), Expect = 0.056
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +3
Query: 69 VQNKENPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSD 115
V + + +V LHGF WR+ L +AG + D G+G SD
Sbjct: 174 VSSGGSAVVFLHGFPEIWYSWRHQMIALADAGFRAVSFDYRGYGLSD 314
>TC232364 weakly similar to GB|AAL11601.1|15983466|AF424607
At1g33990/F12G12_220 {Arabidopsis thaliana;} , partial
(38%)
Length = 476
Score = 34.3 bits (77), Expect = 0.073
Identities = 32/107 (29%), Positives = 48/107 (43%), Gaps = 7/107 (6%)
Frame = +3
Query: 13 KVVAETDADSFPSFLPKDVNRIKDPFARTLATRI---QRLPVSVKFSENPIMSSCVKPLV 69
K+V+E + + +++ F +++ RI +R VS FS N K +
Sbjct: 90 KLVSEEELHLQALSMALQQHQLSQRFEGSMSRRIGSSRRHAVSESFSAN-------KQVP 248
Query: 70 QNKEN----PIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWG 112
N EN VL+HG W T LLEEAG+ A+D+ G G
Sbjct: 249 VNLENIKIKKFVLIHGEGFGAWCWYKTVALLEEAGLLPVALDLTGSG 389
>TC214636 similar to UP|Q39856 (Q39856) Epoxide hydrolase, partial (58%)
Length = 778
Score = 34.3 bits (77), Expect = 0.073
Identities = 21/57 (36%), Positives = 27/57 (46%), Gaps = 1/57 (1%)
Frame = +3
Query: 68 LVQNKENPIVL-LHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCD 123
+ + E P+VL LHGF WR+ L G A D+ G+G D E PS D
Sbjct: 111 IAEKGEGPVVLFLHGFPELWYSWRHQILSLSSLGYRAVAPDLRGYG--DTEAPPSID 275
>TC216299 similar to UP|Q9SSQ3 (Q9SSQ3) F6D8.27 protein, partial (84%)
Length = 1455
Score = 33.9 bits (76), Expect = 0.095
Identities = 15/44 (34%), Positives = 23/44 (52%)
Frame = +3
Query: 72 KENPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSD 115
+ I+ LHG + +R L +AG +A D +G+GFSD
Sbjct: 435 RRGTIIFLHGAPTQSFSYRVVMSQLSDAGFHCFAPDWIGFGFSD 566
>BQ453305
Length = 418
Score = 33.5 bits (75), Expect = 0.12
Identities = 17/40 (42%), Positives = 25/40 (62%), Gaps = 2/40 (5%)
Frame = +2
Query: 185 ATLPRAAAYAGVYLLKSVP--LRVYANYLSFTNISFSTSL 222
AT+ A Y ++L +S P L +YAN+L N+SFS +L
Sbjct: 62 ATVEVATLYVSLFLFRSTPNLLSLYANFLVSVNVSFSRTL 181
>TC218968 similar to GB|AAD39677.1|5103847|F9L1 Contains PF|00561 alpha/beta
hydrolase fold. {Arabidopsis thaliana;} , partial (47%)
Length = 1706
Score = 33.5 bits (75), Expect = 0.12
Identities = 19/52 (36%), Positives = 25/52 (47%), Gaps = 5/52 (9%)
Frame = +1
Query: 68 LVQNKEN----PIVLLHGFDSSCLEWRYTY-PLLEEAGIETWAIDILGWGFS 114
L QN E+ IVL+HGF WR+ PL ++ A D GWG +
Sbjct: 547 LEQNSEDIGQVGIVLIHGFGGGVFSWRHVMTPLARQSNCTVAAFDRPGWGLT 702
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.335 0.145 0.460
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,485,428
Number of Sequences: 63676
Number of extensions: 192172
Number of successful extensions: 1543
Number of sequences better than 10.0: 62
Number of HSP's better than 10.0 without gapping: 1534
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1539
length of query: 307
length of database: 12,639,632
effective HSP length: 97
effective length of query: 210
effective length of database: 6,463,060
effective search space: 1357242600
effective search space used: 1357242600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146567.12