BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146567.1 - phase: 0
(178 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC232788 similar to UP|Q9ZVU9 (Q9ZVU9) T5A14.6 protein, partial ... 280 3e-76
TC213118 30 0.46
TC227300 29 1.3
TC222938 29 1.3
BG359560 28 2.3
TC207817 similar to UP|Q8S8K1 (Q8S8K1) Predicted protein, partia... 28 2.3
BU549476 28 3.0
TC215110 similar to UP|Q9SKU1 (Q9SKU1) Expressed protein (At2g20... 28 3.0
TC226280 similar to UP|EF1G_PRUAV (Q9FUM1) Elongation factor 1-g... 27 3.9
TC230525 27 3.9
BI317183 homologue to GP|14190381|gb| AT5g11200/F2I11_90 {Arabid... 27 5.1
TC230434 similar to UP|Q6TUF4 (Q6TUF4) LRRGT00090, partial (8%) 27 5.1
CO984131 27 5.1
TC203761 similar to UP|Q9FXD7 (Q9FXD7) F12A21.14, partial (22%) 27 5.1
TC224997 homologue to UP|Q7Y199 (Q7Y199) 40S subunit ribosomal p... 27 6.7
TC205570 homologue to UP|Q93VJ8 (Q93VJ8) AT5g11200/F2I11_90 (AT5... 27 6.7
TC226144 weakly similar to UP|Q803P8 (Q803P8) Wu:fb74a06 protein... 26 8.7
TC213341 26 8.7
TC211322 similar to UP|Q8RY22 (Q8RY22) AT3g03380/T21P5_20, parti... 26 8.7
>TC232788 similar to UP|Q9ZVU9 (Q9ZVU9) T5A14.6 protein, partial (39%)
Length = 1069
Score = 280 bits (715), Expect = 3e-76
Identities = 142/163 (87%), Positives = 153/163 (93%)
Frame = +3
Query: 10 LLGSLGASYNGVSLINLAISLFALVAIESSSQSLGRTYAFLLFCSILLDISWFILFTHEI 69
L+GSLGASYNGVSL+NLAI+LFALVAIESSSQSLGRTYAFLLFC+ILLDISWFILF +EI
Sbjct: 3 LIGSLGASYNGVSLVNLAIALFALVAIESSSQSLGRTYAFLLFCAILLDISWFILFANEI 182
Query: 70 WNISSDDYAKFFIFSVKLTLAMQIIGFIVRLSSSLLWIQIYRLGASYVDTASRAADFDLR 129
WNIS+ Y FF FSVKLTLAMQI+GF VRLSSSLLWIQIYRLGASYV+ ASRAAD DLR
Sbjct: 183 WNISTKYYTTFFTFSVKLTLAMQIVGFTVRLSSSLLWIQIYRLGASYVNAASRAADSDLR 362
Query: 130 SSFLSPVTPAVARQISNSNEILGGSIYDPAYYSSLFEDSQENK 172
SSFLSP+TPAVARQ SNSNEILGGSIYDP+YYSSLFED+QENK
Sbjct: 363 SSFLSPMTPAVARQCSNSNEILGGSIYDPSYYSSLFEDAQENK 491
>TC213118
Length = 690
Score = 30.4 bits (67), Expect = 0.46
Identities = 20/76 (26%), Positives = 38/76 (49%)
Frame = -3
Query: 25 NLAISLFALVAIESSSQSLGRTYAFLLFCSILLDISWFILFTHEIWNISSDDYAKFFIFS 84
NL L + + +SQ + + Y +L+ ILL++ FI TH + + Y +
Sbjct: 628 NLPYFLVI*ILLYITSQIIFQVYVYLVSLIILLNLYLFINLTH-FYFVFYKAYNMIL*IN 452
Query: 85 VKLTLAMQIIGFIVRL 100
+K ++ M +I F++ L
Sbjct: 451 LKYSITMMLIYFLITL 404
>TC227300
Length = 2097
Score = 28.9 bits (63), Expect = 1.3
Identities = 15/60 (25%), Positives = 31/60 (51%)
Frame = +2
Query: 113 GASYVDTASRAADFDLRSSFLSPVTPAVARQISNSNEILGGSIYDPAYYSSLFEDSQENK 172
G Y+ + D L ++ L V A + + ++ ++ S+Y P Y ++ E+SQ++K
Sbjct: 44 GDVYMWDGKKGKDKPLVATRLHGVKKATSVSVGETHLLIVASLYHPVYPPNMIENSQKSK 223
>TC222938
Length = 578
Score = 28.9 bits (63), Expect = 1.3
Identities = 26/86 (30%), Positives = 34/86 (39%), Gaps = 7/86 (8%)
Frame = -1
Query: 8 CALLGSLGASYNGVSLINLAISLFAL-VAIESSSQSLGRTYAFLLFCSILLDISWFILFT 66
C LL NG ++ +S F L + S SLG LL+ +L + LF
Sbjct: 278 CPLLLFFSP*LNGPPIVGPLLSYFFLSYTLRPLSLSLGL*GFLLLYTLVLGQTKSYCLFL 99
Query: 67 HEIWNISSDDYAK------FFIFSVK 86
H +W S K FFIF K
Sbjct: 98 HSLW*FCSMGLFKLRKCFIFFIFKYK 21
>BG359560
Length = 351
Score = 28.1 bits (61), Expect = 2.3
Identities = 12/41 (29%), Positives = 23/41 (55%)
Frame = -2
Query: 44 GRTYAFLLFCSILLDISWFILFTHEIWNISSDDYAKFFIFS 84
G ++ FLL+ + +S +I+ E+W ++ FF+FS
Sbjct: 269 GLSFIFLLYPYFPI*VSLYIVLPMEVWEVNVSLLRLFFLFS 147
>TC207817 similar to UP|Q8S8K1 (Q8S8K1) Predicted protein, partial (38%)
Length = 861
Score = 28.1 bits (61), Expect = 2.3
Identities = 15/48 (31%), Positives = 27/48 (56%)
Frame = +1
Query: 48 AFLLFCSILLDISWFILFTHEIWNISSDDYAKFFIFSVKLTLAMQIIG 95
+ L CS+ + F LF ++ ++SSD Y+++ + + T A IIG
Sbjct: 700 SILRICSLFFFLFKFQLFCLKL*HLSSDVYSQYILHASLFTWANLIIG 843
>BU549476
Length = 506
Score = 27.7 bits (60), Expect = 3.0
Identities = 28/98 (28%), Positives = 42/98 (42%), Gaps = 4/98 (4%)
Frame = -1
Query: 48 AFLLFCSILLDISWFILFTHEIWNISSDD----YAKFFIFSVKLTLAMQIIGFIVRLSSS 103
AFL+ ++L+ +S+ FT SSDD +FS K T + I I+ + SS
Sbjct: 416 AFLIVAALLMTVSFTAAFTVPGGVYSSDDPNPKIRGTAVFSGKPTFWIFTIVNIITMYSS 237
Query: 104 LLWIQIYRLGASYVDTASRAADFDLRSSFLSPVTPAVA 141
L + +G SR F L L+ + VA
Sbjct: 236 ALACGLLSMGIFRQSELSRTISFPLLCMMLAFLIAPVA 123
>TC215110 similar to UP|Q9SKU1 (Q9SKU1) Expressed protein
(At2g20760/F5H14.27), partial (47%)
Length = 1225
Score = 27.7 bits (60), Expect = 3.0
Identities = 22/54 (40%), Positives = 23/54 (41%)
Frame = +2
Query: 118 DTASRAADFDLRSSFLSPVTPAVARQISNSNEILGGSIYDPAYYSSLFEDSQEN 171
DT S DF SSF S P + S EI G S P Y S FE EN
Sbjct: 140 DTFSGYPDF---SSFPSDDVPVDYADTAASPEIFGFSDPGPVYSQSPFEQVHEN 292
>TC226280 similar to UP|EF1G_PRUAV (Q9FUM1) Elongation factor 1-gamma
(EF-1-gamma) (eEF-1B gamma), complete
Length = 1703
Score = 27.3 bits (59), Expect = 3.9
Identities = 19/59 (32%), Positives = 30/59 (50%)
Frame = -2
Query: 94 IGFIVRLSSSLLWIQIYRLGASYVDTASRAADFDLRSSFLSPVTPAVARQISNSNEILG 152
IGFI+R S LL I +LGA+ T +A + +FL + R +S + ++ G
Sbjct: 319 IGFILRNSGVLLETPISKLGANSTWTPLYSAAIRVLKAFLFVLVACRIRAMSWTKQVSG 143
>TC230525
Length = 551
Score = 27.3 bits (59), Expect = 3.9
Identities = 18/44 (40%), Positives = 23/44 (51%), Gaps = 5/44 (11%)
Frame = +2
Query: 6 IGCALLGSLGASYN-----GVSLINLAISLFALVAIESSSQSLG 44
+ C L S G S G SL + + SLF+L+ I SS SLG
Sbjct: 110 LSCVSLFSSGVSLFFIFLCGASLFSFSCSLFSLILIWSSHTSLG 241
>BI317183 homologue to GP|14190381|gb| AT5g11200/F2I11_90 {Arabidopsis
thaliana}, partial (14%)
Length = 420
Score = 26.9 bits (58), Expect = 5.1
Identities = 13/44 (29%), Positives = 23/44 (51%)
Frame = -3
Query: 24 INLAISLFALVAIESSSQSLGRTYAFLLFCSILLDISWFILFTH 67
+N I+L A++ SS +G + FLL L + + + F+H
Sbjct: 286 VNSNIALLGSFAVDFSSGGVGGLFVFLLVIEKLFLVCFVLCFSH 155
>TC230434 similar to UP|Q6TUF4 (Q6TUF4) LRRGT00090, partial (8%)
Length = 958
Score = 26.9 bits (58), Expect = 5.1
Identities = 17/45 (37%), Positives = 24/45 (52%)
Frame = +2
Query: 51 LFCSILLDISWFILFTHEIWNISSDDYAKFFIFSVKLTLAMQIIG 95
LFC+ LL ISW + FT + SS + I S+ LT + +G
Sbjct: 449 LFCAFLLHISWLV*FT----DFSSSGFV-V*ILSLTLTSGLACLG 568
>CO984131
Length = 896
Score = 26.9 bits (58), Expect = 5.1
Identities = 16/50 (32%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
Frame = -1
Query: 40 SQSLGRTYAF-LLFCSILLDISWFILFTHEIWNISSDDYAKFFIFSVKLT 88
S + G +F ++F SIL +SW + + N+S + Y K IF+ K++
Sbjct: 359 SMAFGFVISFWVVFGSILFKLSWRHAYFKFLNNLSDNIYVKVAIFANKIS 210
>TC203761 similar to UP|Q9FXD7 (Q9FXD7) F12A21.14, partial (22%)
Length = 1380
Score = 26.9 bits (58), Expect = 5.1
Identities = 8/23 (34%), Positives = 14/23 (60%)
Frame = +3
Query: 48 AFLLFCSILLDISWFILFTHEIW 70
++ + +L+ + W ILFTH W
Sbjct: 1122 SYCCYSFVLVSVVWHILFTHPFW 1190
>TC224997 homologue to UP|Q7Y199 (Q7Y199) 40S subunit ribosomal protein,
partial (27%)
Length = 992
Score = 26.6 bits (57), Expect = 6.7
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = -3
Query: 53 CSILLDISWFILFTHEIWNISSDDYAKFFIFSVKLTLAMQIIG 95
CS LL I +L W IS+D+ K +FS+ L +M +G
Sbjct: 678 CS-LLGIILTVLVFFPSWRISTDNNQKHSLFSICLFFSMIYVG 553
>TC205570 homologue to UP|Q93VJ8 (Q93VJ8) AT5g11200/F2I11_90
(AT5g11170/F2I11_60), partial (98%)
Length = 1745
Score = 26.6 bits (57), Expect = 6.7
Identities = 14/44 (31%), Positives = 22/44 (49%)
Frame = -3
Query: 24 INLAISLFALVAIESSSQSLGRTYAFLLFCSILLDISWFILFTH 67
+N I+L A++ SS +G FLL L + + + FTH
Sbjct: 252 VNSNIALLGSFAVDFSSGGVGGLVVFLLVIEKLFLVCFVLSFTH 121
>TC226144 weakly similar to UP|Q803P8 (Q803P8) Wu:fb74a06 protein, partial
(18%)
Length = 647
Score = 26.2 bits (56), Expect = 8.7
Identities = 10/23 (43%), Positives = 15/23 (64%)
Frame = +3
Query: 65 FTHEIWNISSDDYAKFFIFSVKL 87
FTH+IWN+S + + F VK+
Sbjct: 144 FTHKIWNVS*NSQTRSRSFQVKM 212
>TC213341
Length = 546
Score = 26.2 bits (56), Expect = 8.7
Identities = 10/28 (35%), Positives = 17/28 (60%), Gaps = 2/28 (7%)
Frame = +1
Query: 56 LLDISWFILFTHE--IWNISSDDYAKFF 81
L D++W HE W ++++DY +FF
Sbjct: 31 LCDVAWIDHTGHEETYWRLATEDYIRFF 114
>TC211322 similar to UP|Q8RY22 (Q8RY22) AT3g03380/T21P5_20, partial (18%)
Length = 661
Score = 26.2 bits (56), Expect = 8.7
Identities = 28/141 (19%), Positives = 58/141 (40%)
Frame = +1
Query: 8 CALLGSLGASYNGVSLINLAISLFALVAIESSSQSLGRTYAFLLFCSILLDISWFILFTH 67
C L G + G +I LVA++ ++ ++ + L F + ++I ++F H
Sbjct: 190 CMLDGVHSQHFFGTGVIIYHSQDMGLVAVDKNTVAISASDVMLSFAAFPVEIPGEVVFLH 369
Query: 68 EIWNISSDDYAKFFIFSVKLTLAMQIIGFIVRLSSSLLWIQIYRLGASYVDTASRAADFD 127
+ N + + S + + G +VR + L + R + Y+ SR+
Sbjct: 370 PVHN--------YALISYDPSALGPVGGSVVRAAELLPEPALRRGDSVYLVGLSRSLQAT 525
Query: 128 LRSSFLSPVTPAVARQISNSN 148
R S ++ P A I +++
Sbjct: 526 SRKSVVT--NPCAALNIGSAD 582
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.324 0.137 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,217,587
Number of Sequences: 63676
Number of extensions: 113057
Number of successful extensions: 841
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 841
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 841
length of query: 178
length of database: 12,639,632
effective HSP length: 91
effective length of query: 87
effective length of database: 6,845,116
effective search space: 595525092
effective search space used: 595525092
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146567.1