TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC146564.3 - phase: 0 /pseudo
         (281 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC216233 GB|CAA50573.1|402753|GMFUSA translation elongation fact...   261  7e-75
TC204391 homologue to UP|Q9ASR1 (Q9ASR1) At1g56070/T6H22_13 (Elo...    52  4e-07
TC216234 GB|CAA50573.1|402753|GMFUSA translation elongation fact...    34  0.065
TC229425 similar to UP|Q9FNM5 (Q9FNM5) GTP-binding protein LepA ...    28  3.6
TC228872 similar to UP|SEC8_ARATH (Q93YU5) Probable exocyst comp...    28  3.6
TC223035 similar to UP|Q73IE1 (Q73IE1) Elongation factor Tu fami...    28  4.6

>TC216233 GB|CAA50573.1|402753|GMFUSA translation elongation factor EF-G
            {Glycine max;} , complete
          Length = 2424

 Score =  261 bits (667), Expect(2) = 7e-75
 Identities = 150/267 (56%), Positives = 179/267 (66%), Gaps = 17/267 (6%)
 Frame = +1

Query: 2    DIVTLVGLKDTIAGETLCDPKGPFVLDQMDFPVHVIKIAIEPKTKADMEKMEAGLIKLAE 61
            DI+ L GLKDTI GETLCDP  P VL++MDFP  VIK+AIEPKTKAD++KM  GLIKLA+
Sbjct: 1138 DIIALAGLKDTITGETLCDPDNPIVLERMDFPDPVIKVAIEPKTKADVDKMATGLIKLAQ 1317

Query: 62   EDPSFRVSQDKE-DRTIIQGTGELHLETIVDRLKREFKVEANVGAPQANYRESISEVTE- 119
            EDPSF  S+D+E ++T+I+G GELHLE IVDRLKREFKVEANVGAPQ NYRESIS++   
Sbjct: 1318 EDPSFHFSRDEEINQTVIEGMGELHLEIIVDRLKREFKVEANVGAPQVNYRESISKICRS 1497

Query: 120  -------VRYVFNKPYILNP--------WTQVVDTNLRIKSKKKHFQKNSLYWGF*RIGK 164
                   +R+  +      P        W  V + N R    K+        W   RIG+
Sbjct: 1498 EVCAQETIRWTGSVCRYHCPV*THGPR*WI*VQE*NQRRCCTKRIHS-----WCDERIGR 1662

Query: 165  LHEQWCSSRLSGCWCTRSTCG*YLP*CGFKCEGIPVGSKPSFYGRNEKSQTINA*TYNET 224
            ++EQWC+  LSG  CT ST   +LP C FKC G PVGSK SF GRN+KS T +A* YNE 
Sbjct: 1663 VYEQWCACWLSGR*CTSSTHRWFLPRCRFKCVGFPVGSKRSF*GRNKKSWTKDA*AYNEG 1842

Query: 225  *SCYS*RTL*RCTW*SQQKKRPNHKCW 251
            *SCYS*RT   C W*SQ KKR + + W
Sbjct: 1843 *SCYS*RTSR*CNW*SQLKKRTDQQFW 1923



 Score = 37.4 bits (85), Expect(2) = 7e-75
 Identities = 16/28 (57%), Positives = 22/28 (78%)
 Frame = +2

Query: 253  LTEGRASYSMQFDRFDTVSRNIQNELTT 280
            +T+GRASY+MQ   FD V ++IQN+L T
Sbjct: 2006 MTKGRASYTMQLAMFDVVPQHIQNQLAT 2089


>TC204391 homologue to UP|Q9ASR1 (Q9ASR1) At1g56070/T6H22_13 (Elongation factor
            EF-2), complete
          Length = 2941

 Score = 51.6 bits (122), Expect = 4e-07
 Identities = 37/119 (31%), Positives = 62/119 (52%), Gaps = 6/119 (5%)
 Frame = +1

Query: 4    VTLVGLKDTIA-GETLCDPK--GPFVLDQMDFPVH-VIKIAIEPKTKADMEKMEAGLIKL 59
            V +VGL   I    TL + K      +  M F V  V+++A++ K  +D+ K+  GL +L
Sbjct: 1474 VAMVGLDQFITKNATLTNEKEVDAHPIRAMKFSVSPVVRVAVQCKVASDLPKLVEGLKRL 1653

Query: 60   AEEDPSFRVSQDKEDRTIIQGTGELHLETIVDRLKREFKVEANV--GAPQANYRESISE 116
            A+ DP    + ++    I+ G GELHLE  +  L+ +F   A +    P  ++RE++ E
Sbjct: 1654 AKSDPMVVCTIEESGEHIVAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVLE 1830


>TC216234 GB|CAA50573.1|402753|GMFUSA translation elongation factor EF-G
           {Glycine max;} , partial (5%)
          Length = 402

 Score = 34.3 bits (77), Expect = 0.065
 Identities = 15/25 (60%), Positives = 19/25 (76%)
 Frame = +3

Query: 256 GRASYSMQFDRFDTVSRNIQNELTT 280
           GRASY+MQ   FD V ++IQN+L T
Sbjct: 18  GRASYTMQLAMFDVVPQHIQNQLAT 92


>TC229425 similar to UP|Q9FNM5 (Q9FNM5) GTP-binding protein LepA homolog,
           partial (38%)
          Length = 1009

 Score = 28.5 bits (62), Expect = 3.6
 Identities = 12/30 (40%), Positives = 18/30 (60%)
 Frame = +2

Query: 82  GELHLETIVDRLKREFKVEANVGAPQANYR 111
           G LH+E + +RL+RE+ +     AP   YR
Sbjct: 11  GLLHMEIVQERLEREYNLSLITTAPSVVYR 100


>TC228872 similar to UP|SEC8_ARATH (Q93YU5) Probable exocyst complex
           component Sec8, partial (23%)
          Length = 1260

 Score = 28.5 bits (62), Expect = 3.6
 Identities = 10/19 (52%), Positives = 13/19 (67%)
 Frame = +3

Query: 169 WCSSRLSGCWCTRSTCG*Y 187
           WCS+ LS C+C R+  G Y
Sbjct: 564 WCSANLSKCYCIRTGIGSY 620


>TC223035 similar to UP|Q73IE1 (Q73IE1) Elongation factor Tu family protein,
           partial (13%)
          Length = 443

 Score = 28.1 bits (61), Expect = 4.6
 Identities = 17/64 (26%), Positives = 31/64 (47%)
 Frame = +2

Query: 56  LIKLAEEDPSFRVSQDKEDRTIIQGTGELHLETIVDRLKREFKVEANVGAPQANYRESIS 115
           L+  AE + +  V     +   +QG GEL L  +++ ++RE   E +V  P+  Y+    
Sbjct: 17  LMAEAETNLAINVLPGLSESFEVQGRGELQLGILIENMRRE-GFELSVSPPKVMYKTESG 193

Query: 116 EVTE 119
           +  E
Sbjct: 194 QKLE 205


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.334    0.145    0.477 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,720,836
Number of Sequences: 63676
Number of extensions: 170784
Number of successful extensions: 1480
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1458
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1478
length of query: 281
length of database: 12,639,632
effective HSP length: 96
effective length of query: 185
effective length of database: 6,526,736
effective search space: 1207446160
effective search space used: 1207446160
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 58 (26.9 bits)