BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146561.5 + phase: 2 /pseudo/partial
(263 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC213648 weakly similar to UP|Q7XHN9 (Q7XHN9) Tetratricopeptide ... 442 e-125
BM085131 248 2e-66
CD399839 233 7e-62
CO983443 137 5e-33
AW306578 127 7e-30
CA937139 59 2e-09
BG157401 47 7e-06
TC228958 similar to UP|MCCB_ARATH (Q9LDD8) Methylcrotonyl-CoA ca... 28 4.3
BE805007 homologue to GP|27355823|dbj glycolate oxidase iron-sul... 28 4.3
BE609719 27 7.3
TC224268 27 9.5
TC219732 similar to UP|PEAM_SPIOL (Q9M571) Phosphoethanolamine N... 27 9.5
>TC213648 weakly similar to UP|Q7XHN9 (Q7XHN9) Tetratricopeptide
repeat(TPR)-containing protein-like, partial (19%)
Length = 727
Score = 442 bits (1137), Expect = e-125
Identities = 206/234 (88%), Positives = 222/234 (94%)
Frame = +2
Query: 27 YERTLDIAKTVMKERSYVHGKTDQIIHLSNDGKLEEIMHAKSCSDLYKVVGEDFWSSTWC 86
+ERTL+IAKTVMKERS+VH KTD+IIHLS DGKLEEIMHAK CSDLYKVVGEDFW +TWC
Sbjct: 2 HERTLEIAKTVMKERSFVHSKTDKIIHLSKDGKLEEIMHAKLCSDLYKVVGEDFWLATWC 181
Query: 87 NSTAFEGKQLEGTRVTLVKMGQHGFDFAIRTPCTPARWEDYDAEMAMAWEALCNAYCGEN 146
NSTAFEGKQLEGTR+ LVKMG+HGFDFAI+TPCTPARWED+D EMA+AWE LCNAYCGEN
Sbjct: 182 NSTAFEGKQLEGTRINLVKMGEHGFDFAIKTPCTPARWEDFDEEMAVAWETLCNAYCGEN 361
Query: 147 YGSTDFDVLENVRDAILRMTYYWYNFMPLSRGTAAVGFAVMLGLLLAANMEFTGSIPQGF 206
YGSTDFD LENV DAILRMTYYWYNFMPLSRG+A VGF VMLGLLLAANMEFTGSIPQGF
Sbjct: 362 YGSTDFDTLENVLDAILRMTYYWYNFMPLSRGSAVVGFIVMLGLLLAANMEFTGSIPQGF 541
Query: 207 QVDWEAILNLDPNSFVDSVKSWLYPSLKVTTSWKDYHDVASTFATTGSVVAALS 260
QVDWEAILNLDPNSFVDSVK+WLYPSLKVTTSWKDY D+ASTFATTGSV++AL+
Sbjct: 542 QVDWEAILNLDPNSFVDSVKTWLYPSLKVTTSWKDYPDIASTFATTGSVISALN 703
>BM085131
Length = 370
Score = 248 bits (633), Expect = 2e-66
Identities = 115/123 (93%), Positives = 120/123 (97%)
Frame = +2
Query: 82 SSTWCNSTAFEGKQLEGTRVTLVKMGQHGFDFAIRTPCTPARWEDYDAEMAMAWEALCNA 141
SSTWCNSTAFEGKQLEGTR+TLVKMG++GFDFAIRTPCTPARWEDYDAEMAMAWEALCNA
Sbjct: 2 SSTWCNSTAFEGKQLEGTRITLVKMGENGFDFAIRTPCTPARWEDYDAEMAMAWEALCNA 181
Query: 142 YCGENYGSTDFDVLENVRDAILRMTYYWYNFMPLSRGTAAVGFAVMLGLLLAANMEFTGS 201
YCG+ YGSTDFDVLENVRDAILRMTYYWYNFMPLSRG+A VGF VMLGLLLAANMEFTGS
Sbjct: 182 YCGDFYGSTDFDVLENVRDAILRMTYYWYNFMPLSRGSAVVGFVVMLGLLLAANMEFTGS 361
Query: 202 IPQ 204
IPQ
Sbjct: 362 IPQ 370
>CD399839
Length = 528
Score = 233 bits (594), Expect = 7e-62
Identities = 111/129 (86%), Positives = 120/129 (92%), Gaps = 1/129 (0%)
Frame = -3
Query: 136 EALCNAYCGENYGSTDFDVLENVRDAILRMTYYWYNFMPLSRGTAAVGFAVMLGLLLAAN 195
+ LC+AYCGENYGSTDFD LENVRDAILRMTYYWYNFMPLSRG+ VGF VMLGLLLAAN
Sbjct: 508 QTLCHAYCGENYGSTDFDPLENVRDAILRMTYYWYNFMPLSRGSPGVGFIVMLGLLLAAN 329
Query: 196 MEFTGSIPQGFQVDWEAILNLDPNSFVDSVKSWLYPSLKVTTSWKDYHDVASTFATTGSV 255
MEFTGSIPQG QVDWEA+LNLDPNSFVDSVK+WLYPSLKVTTSWKDY D+ASTFATTGS
Sbjct: 328 MEFTGSIPQGLQVDWEALLNLDPNSFVDSVKTWLYPSLKVTTSWKDYPDIASTFATTGSA 149
Query: 256 VAALS-SYD 263
+AAL+ S+D
Sbjct: 148 LAALNFSFD 122
>CO983443
Length = 779
Score = 137 bits (345), Expect = 5e-33
Identities = 83/163 (50%), Positives = 91/163 (54%)
Frame = -3
Query: 98 GTRVTLVKMGQHGFDFAIRTPCTPARWEDYDAEMAMAWEALCNAYCGENYGSTDFDVLEN 157
G + K G+ GFDFAIRTPCTPARWEDYDAEM MAWE +A
Sbjct: 738 GDKDNTCKNGELGFDFAIRTPCTPARWEDYDAEMPMAWEV*LHA---------------- 607
Query: 158 VRDAILRMTYYWYNFMPLSRGTAAVGFAVMLGLLLAANMEFTGSIPQGFQVDWEAILNLD 217
+ R+ SR GF + P F WE+ILNLD
Sbjct: 606 ---TVPRIC--------CSRICCHAGFVTCC*YGVHRKH------PTRFSG*WESILNLD 478
Query: 218 PNSFVDSVKSWLYPSLKVTTSWKDYHDVASTFATTGSVVAALS 260
PN DSVKSWLYPSLKVTTSWK+YHDVASTFATTGSVVAALS
Sbjct: 477 PNCLGDSVKSWLYPSLKVTTSWKEYHDVASTFATTGSVVAALS 349
Score = 102 bits (254), Expect = 2e-22
Identities = 64/129 (49%), Positives = 70/129 (53%), Gaps = 3/129 (2%)
Frame = -1
Query: 84 TWCNSTAFEGKQLEGTRVTLVKMGQHGFDFAIRTPCTPARWEDYDAEMAMAWEALCNAYC 143
TWCNSTAFEGKQLEGTR+TLVKMG + + E L +
Sbjct: 779 TWCNSTAFEGKQLEGTRITLVKMG--------------------SLVLTLQLEHLVHLLD 660
Query: 144 GENYGSTDFDVLENVRDAILRMTYYW---YNFMPLSRGTAAVGFAVMLGLLLAANMEFTG 200
G I+ W YNFM LSRG+A VGF VMLGLLLAANMEFTG
Sbjct: 659 GR----------------IMMQKCQWHGKYNFMLLSRGSAVVGFVVMLGLLLAANMEFTG 528
Query: 201 SIPQGFQVD 209
SIPQGFQVD
Sbjct: 527 SIPQGFQVD 501
>AW306578
Length = 425
Score = 127 bits (318), Expect = 7e-30
Identities = 60/62 (96%), Positives = 61/62 (97%)
Frame = +3
Query: 202 IPQGFQVDWEAILNLDPNSFVDSVKSWLYPSLKVTTSWKDYHDVASTFATTGSVVAALSS 261
IPQGFQVDWEA+LNLDPNSFVDSVKSWLYPSLKVTTSWKDYHDVASTFATTGSVVAALSS
Sbjct: 6 IPQGFQVDWEAVLNLDPNSFVDSVKSWLYPSLKVTTSWKDYHDVASTFATTGSVVAALSS 185
Query: 262 YD 263
D
Sbjct: 186 SD 191
>CA937139
Length = 422
Score = 58.9 bits (141), Expect = 2e-09
Identities = 29/57 (50%), Positives = 41/57 (71%)
Frame = +1
Query: 8 GSHTPMILGQAKVVRYFPNYERTLDIAKTVMKERSYVHGKTDQIIHLSNDGKLEEIM 64
G + +++G + + + RTLDIAKTV+KE+SYV+ KTDQII L DGKLEE++
Sbjct: 142 GHYL*ILVGSSSGMLILFKHHRTLDIAKTVIKEKSYVYSKTDQIICLFKDGKLEEVV 312
>BG157401
Length = 404
Score = 47.4 bits (111), Expect = 7e-06
Identities = 23/24 (95%), Positives = 23/24 (95%)
Frame = +2
Query: 240 KDYHDVASTFATTGSVVAALSSYD 263
KDYHDVASTFATTGSVVAALSS D
Sbjct: 5 KDYHDVASTFATTGSVVAALSSSD 76
>TC228958 similar to UP|MCCB_ARATH (Q9LDD8) Methylcrotonyl-CoA carboxylase
beta chain, mitochondrial precursor
(3-Methylcrotonyl-CoA carboxylase 2) (MCCase beta
subunit) (3-methylcrotonyl-CoA:carbon dioxide ligase
beta subunit) , partial (63%)
Length = 1226
Score = 28.1 bits (61), Expect = 4.3
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +1
Query: 117 TPCTPARWEDYDAEMAMAWE 136
TPCT A WE+Y+ E A WE
Sbjct: 280 TPCTCA-WEEYN*EFAYGWE 336
>BE805007 homologue to GP|27355823|dbj glycolate oxidase iron-sulfur subunit
{Bradyrhizobium japonicum USDA 110}, partial (4%)
Length = 411
Score = 28.1 bits (61), Expect = 4.3
Identities = 17/48 (35%), Positives = 23/48 (47%), Gaps = 2/48 (4%)
Frame = -2
Query: 216 LDPNSFVDSVKSWLYPSLKVTTSWKDYHDVASTFATT--GSVVAALSS 261
LD V +SWL S SW Y +T ATT +++A L+S
Sbjct: 146 LDMRQMVGLARSWLLDSASSMGSWPGYAWAEATRATTNRAAIMAVLTS 3
>BE609719
Length = 337
Score = 27.3 bits (59), Expect = 7.3
Identities = 12/48 (25%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = +3
Query: 48 TDQIIHLSNDGKLEEIMHAK-SCSDLYKVVGEDFWSSTWCNSTAFEGK 94
T + ++ S++ + + + +C L+++VGE WS C S G+
Sbjct: 183 TQECVNNSDEARAPSLQSCR*NCPKLFEIVGEKPWSKRVCVSPCLGGE 326
>TC224268
Length = 789
Score = 26.9 bits (58), Expect = 9.5
Identities = 19/67 (28%), Positives = 32/67 (47%)
Frame = -3
Query: 175 LSRGTAAVGFAVMLGLLLAANMEFTGSIPQGFQVDWEAILNLDPNSFVDSVKSWLYPSLK 234
+ R +++GF L + +F G+ V + LDP+S V SVK+ L S +
Sbjct: 472 MDRWKSSLGFPE----LASTTTQFPGTTVDSSAVSLSDMAELDPSSVVASVKNLLEESKR 305
Query: 235 VTTSWKD 241
+ +S D
Sbjct: 304 MGSSLGD 284
>TC219732 similar to UP|PEAM_SPIOL (Q9M571) Phosphoethanolamine
N-methyltransferase , partial (48%)
Length = 812
Score = 26.9 bits (58), Expect = 9.5
Identities = 15/55 (27%), Positives = 23/55 (41%)
Frame = +1
Query: 28 ERTLDIAKTVMKERSYVHGKTDQIIHLSNDGKLEEIMHAKSCSDLYKVVGEDFWS 82
+RT KT+ +E + +H K D I ++ EI + GE WS
Sbjct: 526 DRTNLFMKTLQQELNALHSKKDDFIDDFSEEDYNEITERWKAKQMRGADGEQIWS 690
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.134 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,755,052
Number of Sequences: 63676
Number of extensions: 175434
Number of successful extensions: 830
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 825
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 830
length of query: 263
length of database: 12,639,632
effective HSP length: 95
effective length of query: 168
effective length of database: 6,590,412
effective search space: 1107189216
effective search space used: 1107189216
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146561.5