BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146559.4 - phase: 0 /pseudo
(159 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC205690 weakly similar to UP|Q6K3E9 (Q6K3E9) F-box family prote... 152 9e-38
TC206024 weakly similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific le... 148 1e-36
TC232233 weakly similar to GB|AAT41865.1|48310676|BT014882 At1g8... 139 4e-34
TC217151 similar to UP|Q6NPT8 (Q6NPT8) At2g02230, partial (6%) 136 4e-33
TC230781 weakly similar to UP|Q6K3E9 (Q6K3E9) F-box family prote... 127 2e-30
TC228247 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific le... 114 2e-26
TC217152 similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-li... 100 4e-22
BI426662 99 1e-21
BG652227 96 6e-21
BQ610780 96 6e-21
TC217153 similar to GB|AAS76702.1|45773796|BT012215 At1g56240 {A... 90 4e-19
BE803206 73 7e-14
TC228790 67 3e-12
AI440677 62 1e-10
TC207016 56 6e-09
TC207581 56 8e-09
TC207017 55 1e-08
TC228791 49 8e-07
TC228248 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific le... 44 3e-05
TC221119 similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-li... 37 0.003
>TC205690 weakly similar to UP|Q6K3E9 (Q6K3E9) F-box family protein-like,
partial (33%)
Length = 1360
Score = 152 bits (383), Expect = 9e-38
Identities = 79/125 (63%), Positives = 90/125 (71%)
Frame = +2
Query: 15 LPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSLF 74
LPEGCIA ILS T P D CR S+V K F SAA+SD VW RFLPSD SIIS SPS +
Sbjct: 215 LPEGCIASILSRTTPADVCRFSVVSKIFRSAAESDAVWKRFLPSDYHSIISQSPSPLNY- 391
Query: 75 STSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISIALGDTPQFWDWPI 134
PSKK LYL LSD PI+I+ GKKSFQLEK+SG+K YML+AR +SI GDT Q+W+W
Sbjct: 392 ---PSKKELYLALSDRPIIIDQGKKSFQLEKKSGKKCYMLAARALSIIWGDTEQYWNWTT 562
Query: 135 LPESR 139
SR
Sbjct: 563 DTNSR 577
>TC206024 weakly similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-like
protein, partial (47%)
Length = 954
Score = 148 bits (374), Expect = 1e-36
Identities = 78/128 (60%), Positives = 92/128 (70%)
Frame = +3
Query: 12 IEVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSAS 71
++ LPEGCIAKILS+T PVD CRLSLV K F SAA+SDTVWD FL SD SII S
Sbjct: 27 LQDLPEGCIAKILSYTTPVDVCRLSLVSKAFRSAAESDTVWDCFLLSDFTSIIPIS---- 194
Query: 72 SLFSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISIALGDTPQFWD 131
S SKK LY TLSDHP +I G+KS QL+K++G+K MLSAR+++I GDT Q W+
Sbjct: 195 -----STSKKDLYFTLSDHPTIIHQGRKSVQLDKRTGKKCCMLSARNLTIIWGDTVQHWE 359
Query: 132 WPILPESR 139
W LPESR
Sbjct: 360 WTSLPESR 383
>TC232233 weakly similar to GB|AAT41865.1|48310676|BT014882 At1g80110
{Arabidopsis thaliana;} , partial (47%)
Length = 687
Score = 139 bits (351), Expect = 4e-34
Identities = 70/125 (56%), Positives = 91/125 (72%)
Frame = +1
Query: 15 LPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSLF 74
LPEGCIA ILS T+P D CRLSL+ F SAA+SD VW++FLPSD +I+S S S S
Sbjct: 25 LPEGCIANILSFTSPRDVCRLSLLSSTFRSAAQSDAVWNKFLPSDFHTILSQSSSLSL-- 198
Query: 75 STSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISIALGDTPQFWDWPI 134
PSKK L+L L P++I++GKKS+QL+K G+K YMLSAR++ I GDTP++W W
Sbjct: 199 ---PSKKDLFLYLCQKPLLIDDGKKSYQLDKVYGKKCYMLSARNLFIVWGDTPRYWRWTS 369
Query: 135 LPESR 139
LP++R
Sbjct: 370 LPDAR 384
>TC217151 similar to UP|Q6NPT8 (Q6NPT8) At2g02230, partial (6%)
Length = 514
Score = 136 bits (343), Expect = 4e-33
Identities = 75/130 (57%), Positives = 88/130 (67%), Gaps = 5/130 (3%)
Frame = +3
Query: 15 LPEGCIAKILSHTA-PVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSL 73
LPEGC+A ILS+ P D RLSLV K F SAA DTVWDRF+PSD S IS
Sbjct: 36 LPEGCVAHILSYICTPEDIVRLSLVSKAFYSAADYDTVWDRFIPSDFSSTISP------- 194
Query: 74 FSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISI----ALGDTPQF 129
S+S SKK LY TLSD P +I+ G+KSFQLEK++ +K YMLSARDISI G+ Q+
Sbjct: 195 LSSSNSKKDLYFTLSDRPTIIDQGRKSFQLEKRTAKKCYMLSARDISITWAPTQGEASQY 374
Query: 130 WDWPILPESR 139
W+W LPESR
Sbjct: 375 WEWKSLPESR 404
>TC230781 weakly similar to UP|Q6K3E9 (Q6K3E9) F-box family protein-like,
partial (29%)
Length = 971
Score = 127 bits (320), Expect = 2e-30
Identities = 68/113 (60%), Positives = 78/113 (68%)
Frame = +2
Query: 27 TAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSLFSTSPSKKSLYLT 86
T D CRLSLV K F SAA+++TVWD FLPSDL SIIS S S S K LYL
Sbjct: 2 TTRADVCRLSLVSKAFHSAAEANTVWDCFLPSDLSSIISPSSSVPPFRSI----KDLYLY 169
Query: 87 LSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISIALGDTPQFWDWPILPESR 139
LSD P +I+ G KSFQLEK++G K YMLSARD+SI GDT +W+W LPESR
Sbjct: 170 LSDRPTIIDQGIKSFQLEKRTGNKCYMLSARDLSIIWGDTTHYWEWTTLPESR 328
>TC228247 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin
PP2-like protein, partial (46%)
Length = 1124
Score = 114 bits (286), Expect = 2e-26
Identities = 58/128 (45%), Positives = 85/128 (66%)
Frame = +3
Query: 12 IEVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSAS 71
++ LPE C++KILS+T+P D+CR S+V S+A SD +W F PSD I+S + +
Sbjct: 72 MDTLPEDCVSKILSYTSPPDACRFSMVSSTLRSSADSDLLWRTFFPSDYSDIVSRALNPL 251
Query: 72 SLFSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISIALGDTPQFWD 131
SL S+S S K L+ L HP++++ G SF+L+K SG+K Y+LSAR +SI + P +W
Sbjct: 252 SLNSSS-SYKHLFYALC-HPLLLDGGNMSFKLDKSSGKKSYILSARQLSITWSNDPLYWS 425
Query: 132 WPILPESR 139
W +PESR
Sbjct: 426 WRPVPESR 449
>TC217152 similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-like protein,
partial (8%)
Length = 909
Score = 100 bits (248), Expect = 4e-22
Identities = 52/93 (55%), Positives = 63/93 (66%), Gaps = 4/93 (4%)
Frame = +3
Query: 51 VWDRFLPSDLISIISDSPSASSLFSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRK 110
VWDRF+PSD S IS S+S SKK LY TLSD P +I+ G+KSFQLEK++ +K
Sbjct: 3 VWDRFIPSDFSSTISP-------LSSSNSKKDLYFTLSDRPTIIDQGRKSFQLEKRTAKK 161
Query: 111 IYMLSARDISI----ALGDTPQFWDWPILPESR 139
YMLSARDISI G+ Q+W+W LPESR
Sbjct: 162 CYMLSARDISITWAPTQGEASQYWEWKSLPESR 260
>BI426662
Length = 421
Score = 98.6 bits (244), Expect = 1e-21
Identities = 62/136 (45%), Positives = 78/136 (56%), Gaps = 27/136 (19%)
Frame = +3
Query: 13 EVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASS 72
E LPEGCIA I+S T P D+C LSLV F SA+ +D VW+RFLPSD +IIS S S+
Sbjct: 15 EHLPEGCIANIVSFTTPPDACVLSLVSSSFRSASVTDFVWERFLPSDYQAIISQSSKPST 194
Query: 73 LFSTSPSKKSLYLTLSDHPIVIENGKK---------------------------SFQLEK 105
L + S SKK LYL L +P++I+ GKK SF L+K
Sbjct: 195 LTNYS-SKKDLYLHLCHNPLLIDAGKKVLQIKSFFLSIIIHVYLSLIGKLINHQSFALDK 371
Query: 106 QSGRKIYMLSARDISI 121
+G+ YMLSAR +SI
Sbjct: 372 LNGKICYMLSARSLSI 419
>BG652227
Length = 416
Score = 96.3 bits (238), Expect = 6e-21
Identities = 62/136 (45%), Positives = 72/136 (52%), Gaps = 33/136 (24%)
Frame = +3
Query: 15 LPEGCIAKILSHTA-PVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSL 73
LPEGC+A ILS+ P D RLSLV K F SAA DTVWDRF+PSD S IS
Sbjct: 30 LPEGCVAHILSYICTPEDIVRLSLVSKAFYSAADYDTVWDRFIPSDFSSTISP------- 188
Query: 74 FSTSPSKKSLYLTLSDHPIVIENGKK--------------------------------SF 101
S+S SKK LY TLSD P +I+ G+K SF
Sbjct: 189 LSSSNSKKDLYFTLSDRPTIIDQGRKVRTLFLLACSDVFYGIPEMKCVVYVASLKFLQSF 368
Query: 102 QLEKQSGRKIYMLSAR 117
QLEK++ +K YMLSAR
Sbjct: 369 QLEKRTAKKCYMLSAR 416
>BQ610780
Length = 447
Score = 96.3 bits (238), Expect = 6e-21
Identities = 55/100 (55%), Positives = 65/100 (65%)
Frame = +3
Query: 12 IEVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSAS 71
++ LPEGCIAKILS+T PVD+CRLS V F SAA+SDTVWD FL SD S I S
Sbjct: 6 LQDLPEGCIAKILSYTTPVDACRLS-VSIAFRSAAESDTVWDCFLLSDFTSFIPPS---- 170
Query: 72 SLFSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKI 111
S SK LY TLSD P +++ G+KS QL K GR +
Sbjct: 171 -----STSKNDLYFTLSDLPTIMDQGRKSVQLAKGPGRSV 275
Score = 51.2 bits (121), Expect = 2e-07
Identities = 22/47 (46%), Positives = 34/47 (71%)
Frame = +2
Query: 96 NGKKSFQLEKQSGRKIYMLSARDISIALGDTPQFWDWPILPESRCVN 142
+G K + K++G+K YMLSAR+++I GDT + W+W LPES+ V+
Sbjct: 227 SG*KECSIGKRTGKKCYMLSARNLAIIWGDTVRHWEWTSLPESKLVD 367
>TC217153 similar to GB|AAS76702.1|45773796|BT012215 At1g56240 {Arabidopsis
thaliana;} , partial (13%)
Length = 635
Score = 90.1 bits (222), Expect = 4e-19
Identities = 48/89 (53%), Positives = 59/89 (65%), Gaps = 4/89 (4%)
Frame = +3
Query: 55 FLPSDLISIISDSPSASSLFSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYML 114
F+PSD S IS S+S SKK LY TLSD P +I+ G+KSFQLEK++ +K YML
Sbjct: 3 FIPSDFSSTISP-------LSSSNSKKDLYFTLSDRPTIIDQGRKSFQLEKRTAKKCYML 161
Query: 115 SARDISI----ALGDTPQFWDWPILPESR 139
SARDISI G+ Q+W+W LPESR
Sbjct: 162 SARDISITWAPTQGEASQYWEWKSLPESR 248
>BE803206
Length = 404
Score = 72.8 bits (177), Expect = 7e-14
Identities = 43/74 (58%), Positives = 47/74 (63%), Gaps = 1/74 (1%)
Frame = +2
Query: 15 LPEGCIAKILSHTA-PVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSL 73
LPEGC+A ILS+ P D RLSLV K F SAA DTVWDRF+PSD S IS
Sbjct: 29 LPEGCVAHILSYICTPEDIVRLSLVSKAFYSAADYDTVWDRFIPSDFSSTISP------- 187
Query: 74 FSTSPSKKSLYLTL 87
S+S SKK LY TL
Sbjct: 188LSSSNSKKDLYFTL 229
>TC228790
Length = 1215
Score = 67.4 bits (163), Expect = 3e-12
Identities = 38/125 (30%), Positives = 65/125 (51%)
Frame = +2
Query: 15 LPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSLF 74
+PE C+A + H P + C L+ + + F AA SD+VW+ LP + ++ P
Sbjct: 185 IPESCVACVFLHLTPPEICNLARLNRAFRGAASSDSVWEAKLPRNYQDLLDLVPPPER-H 361
Query: 75 STSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISIALGDTPQFWDWPI 134
S SKK ++ LS P+ ++G K L++ +G+ +SA+ + I D ++W+W
Sbjct: 362 HRSLSKKDIFALLS-RPLPFDHGHKEVWLDRVTGKVCMSISAKAMVITGIDDRRYWNWIP 538
Query: 135 LPESR 139
ESR
Sbjct: 539 TEESR 553
>AI440677
Length = 421
Score = 62.0 bits (149), Expect = 1e-10
Identities = 34/121 (28%), Positives = 62/121 (51%)
Frame = +1
Query: 15 LPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSLF 74
+PE C+A++ H P + C L+ + + F AA +D+VW LP + ++ P
Sbjct: 55 IPENCVARVFLHLTPPEICNLARLNRAFRGAASADSVWQTKLPRNYQDLLDLMPPER--- 225
Query: 75 STSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISIALGDTPQFWDWPI 134
+ SKK ++ LS + ++G K L++ + R +SA+ +SI D ++W W +
Sbjct: 226 HRNLSKKDIFALLS-RAVPFDDGNKGVWLDRVTRRVCMSISAKAMSITGIDDRRYWTWFL 402
Query: 135 L 135
L
Sbjct: 403 L 405
>TC207016
Length = 1310
Score = 56.2 bits (134), Expect = 6e-09
Identities = 34/132 (25%), Positives = 68/132 (50%)
Frame = +1
Query: 8 AKSMIEVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDS 67
+++ ++ +PE CI+ ++ + P + C L+ V K F A+ ++ VW+ LP + +++
Sbjct: 295 SETSLDDIPENCISSMMMNFDPQEICSLARVNKTFHRASSANFVWESKLPQNYKFLLNKV 474
Query: 68 PSASSLFSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISIALGDTP 127
+L S +KK +Y L P + G K L++ SG+ +S++ I D
Sbjct: 475 LGEQNL--GSMTKKEIYAKLC-QPNFFDGGTKEVWLDRSSGQVCMFISSKSFKITGIDDR 645
Query: 128 QFWDWPILPESR 139
++W++ ESR
Sbjct: 646 RYWNYIPTEESR 681
>TC207581
Length = 1562
Score = 55.8 bits (133), Expect = 8e-09
Identities = 34/125 (27%), Positives = 62/125 (49%)
Frame = +2
Query: 15 LPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSLF 74
+PE C+A +L + P D C+L+ + + F A+ +D +W+ LP + I+ + S+
Sbjct: 464 IPESCVALVLMYLDPPDICKLARLNRAFRDASSADFIWESKLPLNYKFIVEKALKDVSV- 640
Query: 75 STSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISIALGDTPQFWDWPI 134
K+ +Y L P +NG K L+K++G +S++ + I D ++W
Sbjct: 641 -EQLGKRDIYARLC-RPNSFDNGTKEIWLDKRTGGVCLAISSQALRITGIDDRRYWSRIS 814
Query: 135 LPESR 139
ESR
Sbjct: 815 TEESR 829
>TC207017
Length = 905
Score = 55.1 bits (131), Expect = 1e-08
Identities = 34/125 (27%), Positives = 62/125 (49%)
Frame = +3
Query: 15 LPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSLF 74
+PE CI+ + + P D C+L+ V + F A+ +D VW+ LP + + ++
Sbjct: 189 IPESCISSLFMNLDPPDICKLARVNRAFHRASSADFVWESKLPPSYKFLANKVLGEENI- 365
Query: 75 STSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISIALGDTPQFWDWPI 134
+ +KK +Y L P + G K L+K SG+ +S++ + I D ++W++
Sbjct: 366 -ATMTKKEIYAKLC-LPNRFDGGTKEVWLDKCSGQVCLFMSSKSLKITGIDDRRYWNYIP 539
Query: 135 LPESR 139
ESR
Sbjct: 540 TEESR 554
>TC228791
Length = 584
Score = 49.3 bits (116), Expect = 8e-07
Identities = 31/109 (28%), Positives = 54/109 (49%), Gaps = 6/109 (5%)
Frame = +1
Query: 15 LPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSLF 74
+PE C+A + H P + C L+ + + F AA SD+VW+ LP + ++ P
Sbjct: 268 IPESCVACVFLHLTPPEICNLARLNRAFRGAASSDSVWEAKLPRNYQDLLDLVPPER--- 438
Query: 75 STSPSKKSLYLTLSDHPIVIENGKK------SFQLEKQSGRKIYMLSAR 117
S SKK ++ LS P+ ++G K L++ +G+ +SA+
Sbjct: 439 HRSLSKKDIFALLS-RPLPFDHGHKVGAALQEVWLDRVTGKVCMSISAK 582
>TC228248 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin
PP2-like protein, partial (28%)
Length = 519
Score = 43.9 bits (102), Expect = 3e-05
Identities = 18/36 (50%), Positives = 25/36 (69%)
Frame = +3
Query: 104 EKQSGRKIYMLSARDISIALGDTPQFWDWPILPESR 139
+K SG+K Y+LSAR +SI + P +W W +PESR
Sbjct: 15 DKSSGKKSYILSARQLSITWSNDPLYWSWRPVPESR 122
>TC221119 similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-like protein,
partial (6%)
Length = 971
Score = 37.4 bits (85), Expect = 0.003
Identities = 14/35 (40%), Positives = 23/35 (65%)
Frame = +3
Query: 98 KKSFQLEKQSGRKIYMLSARDISIALGDTPQFWDW 132
K+ +EK +G+ Y+LSAR +SI G++ +W W
Sbjct: 153 KQMLSIEKTTGKICYLLSARQLSITWGNSTLYWSW 257
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.133 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,623,999
Number of Sequences: 63676
Number of extensions: 106035
Number of successful extensions: 713
Number of sequences better than 10.0: 71
Number of HSP's better than 10.0 without gapping: 689
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 690
length of query: 159
length of database: 12,639,632
effective HSP length: 89
effective length of query: 70
effective length of database: 6,972,468
effective search space: 488072760
effective search space used: 488072760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146559.4