BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146554.7 - phase: 0 /pseudo
(1014 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC212032 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, part... 191 2e-61
BQ628592 110 3e-24
BI498328 108 1e-23
TC223727 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, part... 101 1e-21
CF922488 89 7e-18
NP334778 reverse transcriptase [Glycine max] 77 4e-14
AW184779 59 8e-09
TC220664 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial... 56 7e-08
TC233372 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial... 53 8e-07
TC233837 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, part... 51 2e-06
NP395547 reverse transcriptase [Glycine max] 37 0.034
NP395548 reverse transcriptase [Glycine max] 35 0.22
TC203464 similar to GB|AAF35920.1|7025820|AC005892 L505.8 {Leish... 34 0.37
TC219663 similar to GB|AAA36308.1|386949|HUMMHRD5 MHC HLA-RD pro... 30 7.0
TC209955 29 9.1
>TC212032 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, partial (3%)
Length = 803
Score = 191 bits (486), Expect(2) = 2e-61
Identities = 94/194 (48%), Positives = 130/194 (66%)
Frame = +2
Query: 798 IEEAIDLRIKKIVIYGDLALVINQIKGEWETRHPGLIPYRDYARRLLTFFNKVELHHVPR 857
++ AID +K + +YGD ALVI+Q++GE ETR P LIPY+ Y + L FF+++ HHV
Sbjct: 206 VQAAIDSNVKLLKVYGDSALVIHQLRGECETRDPNLIPYQAYIKELAGFFDEISFHHVA* 385
Query: 858 DENQMADALATLSSMINVNGHNTVLVINVQFLDRPAYVFAAEAVDDDKPWYHDIQVFVQT 917
+ENQMADALATL SM + H + I + RPA+ E D KPWY DI+ +V++
Sbjct: 386 EENQMADALATLVSMFQLTPHGDLPYIEFRCRGRPAHCCLVEEERDGKPWYFDIKRYVES 565
Query: 918 QKYPPGASNKDKKTLRKLSSRFFLNEDVLYKRNFDGVLLRCVNKHEAGKLMHEIHEGSFG 977
++YP AS+ DK+ R+L++ FF++ +LYKRN D VLL CVN E ++ E+HEGSFG
Sbjct: 566 KEYPLEASDNDKRRKRRLAAGFFMSGSILYKRNHDMVLLHCVNGKEVENMLGEVHEGSFG 745
Query: 978 THSCGHAKL*RSLR 991
THS GHA + LR
Sbjct: 746 THSNGHAMARKILR 787
Score = 64.3 bits (155), Expect(2) = 2e-61
Identities = 27/46 (58%), Positives = 34/46 (73%)
Frame = +1
Query: 751 FDGAVNVYGSGIGAVLITPKGTHIPFTARIQFDCTNNIVEYEACIM 796
FD A NV G G+GA+L++P IPFT R+ FDCTNN+ EYEAC +
Sbjct: 64 FDRASNVLGHGVGAILVSPDNQCIPFTTRLGFDCTNNMAEYEACAL 201
>BQ628592
Length = 423
Score = 110 bits (275), Expect = 3e-24
Identities = 56/106 (52%), Positives = 70/106 (65%)
Frame = -3
Query: 582 RKAVDHVFDCVR*FYGLCAWSTRRDWKERACHLLLE*EVH*L*VPIFCT*ENLLRFAWAA 641
RK V D VR YG+ S RR W++ HLL + EV+ L + WA+
Sbjct: 319 RKTFSPVHDYVRRVYGMRVGSARRLWEKGTSHLLFKQEVYRLKMNYSMLERTCCTLVWAS 140
Query: 642 KHLRHYMVNHTTWLMSKMDPIKYIFEKPALTGRIARWQMLLSEYDI 687
LR YM++HTTWL+SKMDP+KYIFEKPALTGRIARWQ+LLSE++I
Sbjct: 139 HRLRQYMLSHTTWLISKMDPVKYIFEKPALTGRIARWQVLLSEFNI 2
>BI498328
Length = 335
Score = 108 bits (270), Expect = 1e-23
Identities = 56/118 (47%), Positives = 80/118 (67%), Gaps = 1/118 (0%)
Frame = +1
Query: 691 TQKAIKGSILANHLAHQPIEDYQPIKFDFPDKEIMYL-KMKDCDEPVFGEGPDPESE*GL 749
TQKA+KGS LA++LA P++ Y+P+ +FPD++IM L + K E + ++ +
Sbjct: 4 TQKAVKGSALADYLAQ*PLQGYRPMHPEFPDEDIMALFEEKRTHEDI--------NKWIV 159
Query: 750 IFDGAVNVYGSGIGAVLITPKGTHIPFTARIQFDCTNNIVEYEACIMGIEEAIDLRIK 807
FDGA N G G+GAVL++P IPFTAR+ FDCTNN+ EYEAC +G++ AID +K
Sbjct: 160 CFDGASNALGHGVGAVLVSPDDQCIPFTARLGFDCTNNMAEYEACALGVQAAIDFDVK 333
>TC223727 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, partial (9%)
Length = 843
Score = 101 bits (252), Expect = 1e-21
Identities = 49/113 (43%), Positives = 72/113 (63%)
Frame = +1
Query: 891 RPAYVFAAEAVDDDKPWYHDIQVFVQTQKYPPGASNKDKKTLRKLSSRFFLNEDVLYKRN 950
+PA+ E D KPWY DI+ +V +++Y P ++ DK+TLR+L++ FF++ +LYKRN
Sbjct: 91 KPAHCCQVEEERDGKPWYFDIKRYVISKEYLPEIADNDKRTLRRLAAGFFMSGSILYKRN 270
Query: 951 FDGVLLRCVNKHEAGKLMHEIHEGSFGTHSCGHAKL*RSLRGEESFLWWCFNS 1003
D LRCV+ EA ++ E+HEGSFGTH+ GHA + LR + W S
Sbjct: 271 HDMKPLRCVDAREANHMIEEVHEGSFGTHANGHAMARKILRA--GYYWLTMES 423
>CF922488
Length = 741
Score = 89.4 bits (220), Expect = 7e-18
Identities = 76/238 (31%), Positives = 120/238 (49%)
Frame = +2
Query: 337 WQSQNVCGLPGLEQG*SEG*FPFTSH*CIG**HC*KQGFLLHGQFLRLQSDQDGS*R*RK 396
W+ NV GL + S+G* FT++ *H LLHG RL D+D + K
Sbjct: 17 WEGVNVRGLSRSKLSQSKG*VSFTAYQRSRG*HDQFFPILLHGWIFRL*PDKDSTRGYGK 196
Query: 397 DVFHHTLGCFLLCGDAIWVDKCRCHLPKGYDQKFS*HDSQRNRGICG*YDCQIRH*RKTC 456
D FH+++G LL G +W ++C ++P G+ HD+Q +RG+ G*+D +I++ T
Sbjct: 197 DNFHYSMGNLLL*GYVVWAEECWGNIPAGHGGIILGHDAQGDRGLHG*HDREIKNGGGTP 376
Query: 457 *VFVEDVPTVEKVQTPFES*QVYLRC*IRQTPGLYCQPERY*SRSQQS*SYQGNACSKD* 516
F + V +Q ES +VY+ I++ LY ER +Q S + +
Sbjct: 377 CQFAKVV*ATT*IQVETESRKVYV*GKIQKVARLY**LERNRGGFEQGESDP*DGQATYR 556
Query: 517 EAS*RFFRKVELYLQIHLSYDCHMWADIQVTQ*RSKDRLDRRLPESF**HPRVFVRTS 574
EAS RF +VEL+ +IH+ +CH+ A + S ++ L S * + VF ++S
Sbjct: 557 EASPRFLGEVELHRKIHIIVNCHLRASFHIVVQESVCQMGP*LLSSI*EN*TVFNKSS 730
>NP334778 reverse transcriptase [Glycine max]
Length = 431
Score = 77.0 bits (188), Expect = 4e-14
Identities = 52/142 (36%), Positives = 77/142 (53%)
Frame = +2
Query: 340 QNVCGLPGLEQG*SEG*FPFTSH*CIG**HC*KQGFLLHGQFLRLQSDQDGS*R*RKDVF 399
++VCGL + S+G*F FT+H *H L HG L SD+DG+ KD F
Sbjct: 2 EDVCGLSRSKPSQSKG*FSFTAHRHSHG*HGQFCPILFHGWIFGL*SDKDGTRGYGKDNF 181
Query: 400 HHTLGCFLLCGDAIWVDKCRCHLPKGYDQKFS*HDSQRNRGICG*YDCQIRH*RKTC*VF 459
H+++G LL GD +W ++ +LP G+ HD+Q +RG+CG* D +I++ T F
Sbjct: 182 HYSMGNLLL*GDVVWAEEFWGNLPSGHGGIIPGHDAQGDRGLCG*NDREIKNGGGTPSQF 361
Query: 460 VEDVPTVEKVQTPFES*QVYLR 481
+ V +Q ES +V +R
Sbjct: 362 AKFVWATT*IQVETESQEVCVR 427
>AW184779
Length = 432
Score = 59.3 bits (142), Expect = 8e-09
Identities = 28/51 (54%), Positives = 38/51 (73%)
Frame = +1
Query: 941 LNEDVLYKRNFDGVLLRCVNKHEAGKLMHEIHEGSFGTHSCGHAKL*RSLR 991
L+ ++LYKRN D VLLRCV+ EA +++ E+HEGSFGTH+ HA + LR
Sbjct: 1 LSRNILYKRNHDMVLLRCVDAREAEQMLVEVHEGSFGTHANIHAMAQKILR 153
>TC220664 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial (31%)
Length = 724
Score = 56.2 bits (134), Expect = 7e-08
Identities = 32/86 (37%), Positives = 46/86 (53%)
Frame = +1
Query: 785 TNNIVEYEACIMGIEEAIDLRIKKIVIYGDLALVINQIKGEWETRHPGLIPYRDYARRLL 844
TNN EY A I+G++ A+ I I GD LV QI G W+ ++ L + A+ L
Sbjct: 64 TNNAAEYRAMILGMKYALKKGFTGIRIQGDSKLVCMQIDGSWKVKNENLSTLYNVAKELK 243
Query: 845 TFFNKVELHHVPRDENQMADALATLS 870
F+ ++ HV R+ N ADA A L+
Sbjct: 244 DKFSSFQISHVLRNFNSDADAQANLA 321
>TC233372 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial (12%)
Length = 561
Score = 52.8 bits (125), Expect = 8e-07
Identities = 27/81 (33%), Positives = 43/81 (52%)
Frame = +1
Query: 790 EYEACIMGIEEAIDLRIKKIVIYGDLALVINQIKGEWETRHPGLIPYRDYARRLLTFFNK 849
EY + I+G+ K I++ GD LV NQI+G W+ ++ + A+ L F
Sbjct: 4 EYRSLILGLXHXXKKGYKXIIVQGDSLLVCNQIQGLWKIKNQNMGTLCXEAKELKDKFLS 183
Query: 850 VELHHVPRDENQMADALATLS 870
++ H+PR+ N ADA A L+
Sbjct: 184 FKISHIPREYNSEADAQANLA 246
>TC233837 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, partial (6%)
Length = 402
Score = 51.2 bits (121), Expect = 2e-06
Identities = 40/130 (30%), Positives = 69/130 (52%)
Frame = +1
Query: 375 FLLHGQFLRLQSDQDGS*R*RKDVFHHTLGCFLLCGDAIWVDKCRCHLPKGYDQKFS*HD 434
FL++G FL + S+ DG+*R R+D F H +G L D + +K +L + *+D
Sbjct: 1 FLIYGWFLGV*SNIDGT*RCREDHFRHPMGDIQL*SDGLRAEKYWGNLSACHGGVVP*YD 180
Query: 435 SQRNRGICG*YDCQIRH*RKTC*VFVEDVPTVEKVQTPFES*QVYLRC*IRQTPGLYCQP 494
+ NRG+ *+DCQ+ * +T V V V ++ + Q+++ + + +Y +
Sbjct: 181 A*GNRGLRR*HDCQVSD*DRTPCQSV*AVWKVAEIPIKTKPNQMHIWGEVGEAARIYRKS 360
Query: 495 ERY*SRSQQS 504
ER RS++S
Sbjct: 361 ERDRDRSRES 390
>NP395547 reverse transcriptase [Glycine max]
Length = 762
Score = 37.4 bits (85), Expect = 0.034
Identities = 24/67 (35%), Positives = 34/67 (49%)
Frame = +3
Query: 379 GQFLRLQSDQDGS*R*RKDVFHHTLGCFLLCGDAIWVDKCRCHLPKGYDQKFS*HDSQRN 438
G+ LRLQSD GS RK+ F+ + CF L A+ C + + YD F *H +
Sbjct: 279 GRILRLQSDCSGSSGSRKNSFYMSFQCFCLSPHAVRFM*CLYYFSEMYDGNF**HGREMY 458
Query: 439 RGICG*Y 445
+ G*+
Sbjct: 459 *SLYG*F 479
>NP395548 reverse transcriptase [Glycine max]
Length = 762
Score = 34.7 bits (78), Expect = 0.22
Identities = 30/100 (30%), Positives = 43/100 (43%)
Frame = +3
Query: 345 LPGLEQG*SEG*FPFTSH*CIG**HC*KQGFLLHGQFLRLQSDQDGS*R*RKDVFHHTLG 404
LP ++ E FP T H C + LL G L + SD +D H
Sbjct: 177 LPQAQRSHKERPFPSTLHGSDVGETCRTRLLLLLGCILWI*SDCCRPQGSGEDGLHMPFW 356
Query: 405 CFLLCGDAIWVDKCRCHLPKGYDQKFS*HDSQRNRGICG* 444
C L D+IWV +C H+P + F + +++R I G*
Sbjct: 357 CLCL*TDSIWVVQCTYHIPNVHVGHFCRYSGEKHRSIHG* 476
>TC203464 similar to GB|AAF35920.1|7025820|AC005892 L505.8 {Leishmania
major;} , partial (8%)
Length = 1386
Score = 33.9 bits (76), Expect = 0.37
Identities = 22/80 (27%), Positives = 41/80 (50%), Gaps = 2/80 (2%)
Frame = +2
Query: 790 EYEACIMGIEEAIDLRIKKIVIYGDLALVINQIKGEWETRHP--GLIPYRDYARRLLTFF 847
E EA + G++ A ++ +KK+++ D V++ ++ + HP G++ RR F
Sbjct: 653 ELEAILTGLKVASEMNVKKLIVESDSDSVVSMVENGVKPNHPDYGVVELIRTKRR--RFD 826
Query: 848 NKVELHHVPRDENQMADALA 867
+V V N++AD LA
Sbjct: 827 WEVRFVSVSNKANRVADRLA 886
>TC219663 similar to GB|AAA36308.1|386949|HUMMHRD5 MHC HLA-RD protein {Homo
sapiens;} , partial (14%)
Length = 1176
Score = 29.6 bits (65), Expect = 7.0
Identities = 22/79 (27%), Positives = 34/79 (42%), Gaps = 4/79 (5%)
Frame = +1
Query: 748 GLIFDGAVNVYGSGIGAVLITPKGTHIPFTARIQFDCTNNIVEYEACIMGIEEAID---- 803
G++ G + +GIG T G I I+ + IV ++GIE ID
Sbjct: 355 GIVIIGRIGTTETGIGTGTETETGKEIEIVGVIEIEHGTVIVSEGGTVIGIENMIDIVRG 534
Query: 804 LRIKKIVIYGDLALVINQI 822
+ I K+V + A VI +
Sbjct: 535 IEIMKLVTLIEDAHVIGSL 591
>TC209955
Length = 819
Score = 29.3 bits (64), Expect = 9.1
Identities = 13/36 (36%), Positives = 18/36 (49%)
Frame = -1
Query: 948 KRNFDGVLLRCVNKHEAGKLMHEIHEGSFGTHSCGH 983
+RNF LLRC KH G ++ G ++C H
Sbjct: 303 QRNFFPFLLRCKPKHFDGSSRSQVQSGYSTKYNCSH 196
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.352 0.157 0.561
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,209,276
Number of Sequences: 63676
Number of extensions: 721228
Number of successful extensions: 6935
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 5114
Number of HSP's successfully gapped in prelim test: 176
Number of HSP's that attempted gapping in prelim test: 1705
Number of HSP's gapped (non-prelim): 5470
length of query: 1014
length of database: 12,639,632
effective HSP length: 107
effective length of query: 907
effective length of database: 5,826,300
effective search space: 5284454100
effective search space used: 5284454100
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (22.0 bits)
S2: 64 (29.3 bits)
Medicago: description of AC146554.7