BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146553.6 + phase: 0 /pseudo
(799 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC230382 similar to UP|Q8RY23 (Q8RY23) AT5g18860/F17K4_110, part... 180 e-106
TC229142 similar to UP|Q8RY23 (Q8RY23) AT5g18860/F17K4_110, part... 132 1e-63
BF425902 104 2e-34
TC227677 51 2e-06
CD408835 41 0.002
BE657185 40 0.003
TC216452 similar to UP|O81398 (O81398) Fructose-6-phosphate 2-ki... 32 1.1
>TC230382 similar to UP|Q8RY23 (Q8RY23) AT5g18860/F17K4_110, partial (26%)
Length = 1047
Score = 180 bits (456), Expect(3) = e-106
Identities = 90/116 (77%), Positives = 100/116 (85%), Gaps = 2/116 (1%)
Frame = +1
Query: 480 FFAMNQSN--FSPVGDCKYVKAIPHGNGGFLDSDTLFGLARDLPRSPRRYTAENSKKFGA 537
FF MNQS+ F G+CKYVKAIPHGNGGFLDSDTL+GLARDLPRSPRRYT ENS KF
Sbjct: 163 FFPMNQSDPIFPTAGNCKYVKAIPHGNGGFLDSDTLYGLARDLPRSPRRYTLENSMKFET 342
Query: 538 PRDTDHPELRQPQAMEIWESILQTLKPGSKVTVLTNGPLTNLAKVVSIKNISSIIQ 593
++TDH ELRQP AMEIWESI+QTL+P SK+TVLTNGPLT LAKVVS+KNISS I+
Sbjct: 343 SQNTDHLELRQPLAMEIWESIMQTLEPESKITVLTNGPLTTLAKVVSMKNISSRIE 510
Score = 142 bits (358), Expect(3) = e-106
Identities = 73/103 (70%), Positives = 81/103 (77%)
Frame = +3
Query: 602 GGLCNGRTYQQE*Q*QRKCIFCSFQQVCRIQYVLGSFSSKDSVSIRS*HHTHSTRYPAQS 661
GGLCNG T+QQE *QRK IFCSFQ +CRI YV GSFSS+DSVSIRS*HHTHST+ P +S
Sbjct: 510 GGLCNGGTHQQECL*QRKYIFCSFQPICRI*YVPGSFSSQDSVSIRS*HHTHSTQCPTES 689
Query: 662 KFIFKYFKLA*SDRKNTRSSVFQASSVKATPFEENPPQISPYG 704
KFIF Y L DR NT + VFQA ++KAT E NPPQISPYG
Sbjct: 690 KFIFTYVMLVA*DRTNT*NCVFQACAIKATTSEANPPQISPYG 818
Score = 103 bits (257), Expect(3) = e-106
Identities = 51/56 (91%), Positives = 53/56 (94%)
Frame = +3
Query: 427 LALSYLLKVPVEVINLKAIIVSPTGWANAATIDVIYDLLHMMGRDDIKVGIGDFFA 482
LAL YLLKV VEVINLKAIIVSPTGWANAATIDVIYDLLHMMGRDDI VG+GDFF+
Sbjct: 3 LALFYLLKVDVEVINLKAIIVSPTGWANAATIDVIYDLLHMMGRDDIPVGLGDFFS 170
Score = 79.7 bits (195), Expect(2) = 2e-16
Identities = 59/176 (33%), Positives = 84/176 (47%), Gaps = 20/176 (11%)
Frame = +1
Query: 40 TAGYCRYRQAIPVGFGGRLDIDTNLGIRKAFLPQGKRKYT----------------PLRQ 83
TAG C+Y +AIP G GG LD DT G+ + LP+ R+YT LRQ
Sbjct: 199 TAGNCKYVKAIPHGNGGFLDSDTLYGLARD-LPRSPRRYTLENSMKFETSQNTDHLELRQ 375
Query: 84 PTTQQVLIDKISA----GPITLIVTGAHTNLAIFLMNNPHLKKNVEHVYIMGGVIRSKTC 139
P ++ + IT++ G T LA +++ ++ +E VY+MGG I C
Sbjct: 376 PLAMEIWESIMQTLEPESKITVLTNGPLTTLAK-VVSMKNISSRIEEVYVMGGHISRNVC 552
Query: 140 CTKNASSSCIPSKCGDTGNVLTNYNANPYAEYNIFGDPFAAYKVIHSGIPITLVPL 195
D GN+ + +N YAE+N+F DP AA V S + ITL+PL
Sbjct: 553 ---------------DKGNIFS-VPSNQYAEFNMFLDPLAAKTVFQSEVNITLIPL 672
Score = 25.0 bits (53), Expect(2) = 2e-16
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +3
Query: 1 MMGRDDIAVGVG 12
MMGRDDI VG+G
Sbjct: 123 MMGRDDIPVGLG 158
>TC229142 similar to UP|Q8RY23 (Q8RY23) AT5g18860/F17K4_110, partial (17%)
Length = 1062
Score = 132 bits (333), Expect(2) = 1e-63
Identities = 64/78 (82%), Positives = 68/78 (87%)
Frame = +3
Query: 516 LARDLPRSPRRYTAENSKKFGAPRDTDHPELRQPQAMEIWESILQTLKPGSKVTVLTNGP 575
LARDLPRSPRRYTAENS KFGAPRDTDHPELRQP AME+W S+LQ KP SK+TVLTNGP
Sbjct: 3 LARDLPRSPRRYTAENSVKFGAPRDTDHPELRQPLAMEVWNSVLQRTKPRSKITVLTNGP 182
Query: 576 LTNLAKVVSIKNISSIIQ 593
LT L KVVS+KNI S IQ
Sbjct: 183 LTXLVKVVSVKNIRSTIQ 236
Score = 130 bits (326), Expect(2) = 1e-63
Identities = 66/118 (55%), Positives = 87/118 (72%)
Frame = +2
Query: 599 NVSGGLCNGRTYQQE*Q*QRKCIFCSFQQVCRIQYVLGSFSSKDSVSIRS*HHTHSTRYP 658
N SGG+C+G T++Q+ Q*QR+ +F SF+ +CRIQYV GSFSS+DSV IRS*HHTH T++
Sbjct: 227 NNSGGICSGGTHKQQCQ*QRRYLFSSFEPICRIQYVPGSFSSQDSV*IRS*HHTHPTQHT 406
Query: 659 AQSKFIFKYFKLA*SDRKNTRSSVFQASSVKATPFEENPPQISPYGAVVLANGHSSLL 716
AQSK IF +++ + KNTR +F AS+VKA PF+ NP QIS YG ++ N S L
Sbjct: 407 AQSKIIFNHYRRVA*NTKNTRGCIF*ASAVKAIPFKANP*QISAYGHILGGNSRRSCL 580
Score = 128 bits (321), Expect = 1e-29
Identities = 63/88 (71%), Positives = 74/88 (83%)
Frame = +3
Query: 704 GAVVLANGHSSLLDAKFELKSVKLLAEGIESTDGKMVVDEKYGKLVRILRHVDAKTYHEI 763
GAVVLA HS L + KFE K+VK+LA+G ES+DGK VVDEK GKL+RIL VDAK YH +
Sbjct: 567 GAVVLAESHSGL-NPKFEAKAVKVLADGNESSDGKTVVDEKGGKLMRILSSVDAKAYHSL 743
Query: 764 YAKRLGDPNQSAKVGSFKEQKRKWSHPH 791
YAK+LGD NQSAK+GSF+EQ+RKWSHPH
Sbjct: 744 YAKKLGDENQSAKIGSFEEQRRKWSHPH 827
Score = 53.9 bits (128), Expect = 3e-07
Identities = 49/208 (23%), Positives = 87/208 (41%), Gaps = 20/208 (9%)
Frame = +3
Query: 71 LPQGKRKYTP----------------LRQPTTQQVLIDKISA----GPITLIVTGAHTNL 110
LP+ R+YT LRQP +V + IT++ G T L
Sbjct: 15 LPRSPRRYTAENSVKFGAPRDTDHPELRQPLAMEVWNSVLQRTKPRSKITVLTNGPLTXL 194
Query: 111 AIFLMNNPHLKKNVEHVYIMGGVIRSKTCCTKNASSSCIPSKCGDTGNVLTNYNANPYAE 170
+ +++ +++ ++ VY++GG I S D G++ + +N YAE
Sbjct: 195 -VKVVSVKNIRSTIQEVYVVGGHISSNV---------------NDKGDIFS-VPSNQYAE 323
Query: 171 YNIFGDPFAAYKVIHSGIPITLVPLDATNTIPISEEFFDEFEKSQDTYEAQYCFKSLKMA 230
+N+F DP AA V S + ITL+PL+ + E ++ T EA + + L
Sbjct: 324 FNMFLDPLAAKIVFESEVNITLIPLNTQRRVRSFSTIIGELRRTPRTPEAVFSERLLSRL 503
Query: 231 HDTWFDNQFYTSYFMWDSFTSGVAVSIM 258
+ Q + Y D+F + +++
Sbjct: 504 YRL---KQTHNRYQHMDTFLGEILGAVV 578
>BF425902
Length = 391
Score = 104 bits (260), Expect(2) = 2e-34
Identities = 54/76 (71%), Positives = 65/76 (85%)
Frame = +3
Query: 704 GAVVLANGHSSLLDAKFELKSVKLLAEGIESTDGKMVVDEKYGKLVRILRHVDAKTYHEI 763
GA+VLA+G SSL + KFE+K +K+LAEG ES DG+MVVDE++GKLVRIL HV+ K YHE
Sbjct: 165 GAIVLADGPSSLKE-KFEIKGIKVLAEGDESIDGEMVVDEEHGKLVRILSHVNVKAYHEK 341
Query: 764 YAKRLGDPNQSAKVGS 779
Y+KRLGD NQSAKVGS
Sbjct: 342 YSKRLGDWNQSAKVGS 389
Score = 60.8 bits (146), Expect(2) = 2e-34
Identities = 30/53 (56%), Positives = 36/53 (67%)
Frame = +2
Query: 658 PAQSKFIFKYFKLA*SDRKNTRSSVFQASSVKATPFEENPPQISPYGAVVLAN 710
P +SKFIF Y L DR NT + VFQA ++KAT E NPPQISPYG ++ N
Sbjct: 2 PTKSKFIFTYVMLVA*DRTNT*NCVFQACAIKATTSEANPPQISPYGHILRGN 160
>TC227677
Length = 1543
Score = 50.8 bits (120), Expect = 2e-06
Identities = 40/156 (25%), Positives = 69/156 (43%), Gaps = 2/156 (1%)
Frame = +3
Query: 65 GIRKAFLPQGKRKYTPLRQPTTQQVLIDKISA--GPITLIVTGAHTNLAIFLMNNPHLKK 122
G+ FLP K + + + + L++K+S G ++++ G TNLA+ + +
Sbjct: 471 GLGNTFLPPPKGEKI---EKSASEFLVEKVSEYPGEVSVLALGPLTNLALAIKRDSSFAS 641
Query: 123 NVEHVYIMGGVIRSKTCCTKNASSSCIPSKCGDTGNVLTNYNANPYAEYNIFGDPFAAYK 182
V+ + I+GG + GNV NP AE NI GDP AA
Sbjct: 642 KVKRIVILGGAFFA-------------------LGNV------NPAAEANIHGDPEAADI 746
Query: 183 VIHSGIPITLVPLDATNTIPISEEFFDEFEKSQDTY 218
V SG I ++ ++ T + ++ E ++S+ Y
Sbjct: 747 VFTSGADIVVIGINITTQVQFTDADLLELKESKGKY 854
>CD408835
Length = 644
Score = 41.2 bits (95), Expect = 0.002
Identities = 25/66 (37%), Positives = 35/66 (52%)
Frame = -3
Query: 162 NYNANPYAEYNIFGDPFAAYKVIHSGIPITLVPLDATNTIPISEEFFDEFEKSQDTYEAQ 221
N N NP AE N FGDP AA V SG + V ++ T+ + ++E ++ S + AQ
Sbjct: 633 NGNVNPAAEANTFGDPDAADVVFTSGADVLAVGINVTHQVVLTESDREKLASSNGKF-AQ 457
Query: 222 YCFKSL 227
Y K L
Sbjct: 456 YLNKIL 439
>BE657185
Length = 637
Score = 40.4 bits (93), Expect = 0.003
Identities = 24/64 (37%), Positives = 34/64 (52%)
Frame = -1
Query: 164 NANPYAEYNIFGDPFAAYKVIHSGIPITLVPLDATNTIPISEEFFDEFEKSQDTYEAQYC 223
N NP AE NIFGDP AA V SG + V ++ T+ + ++E ++ S + QY
Sbjct: 637 NVNPAAEANIFGDPEAADVVFTSGADVLAVGINVTHQVVLTESDREKLASSNGKF-VQYL 461
Query: 224 FKSL 227
K L
Sbjct: 460 NKIL 449
>TC216452 similar to UP|O81398 (O81398) Fructose-6-phosphate
2-kinase/fructose-2,6-bisphosphatase (Fragment) ,
partial (68%)
Length = 1249
Score = 32.0 bits (71), Expect = 1.1
Identities = 20/67 (29%), Positives = 31/67 (45%)
Frame = +3
Query: 546 LRQPQAMEIWESILQTLKPGSKVTVLTNGPLTNLAKVVSIKNISSIIQA*QFQNVSGGLC 605
L+ +A IW S LQ T+LT GP+ K IQ ++ G+C
Sbjct: 582 LKSERAACIWTSTLQR-------TILTAGPIVGFPK----------IQWRALDEINAGVC 710
Query: 606 NGRTYQQ 612
+G+TY++
Sbjct: 711 DGKTYEE 731
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.139 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,854,609
Number of Sequences: 63676
Number of extensions: 466271
Number of successful extensions: 2152
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 2130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2147
length of query: 799
length of database: 12,639,632
effective HSP length: 105
effective length of query: 694
effective length of database: 5,953,652
effective search space: 4131834488
effective search space used: 4131834488
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 63 (28.9 bits)
Medicago: description of AC146553.6