BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146553.5 - phase: 1 /pseudo
(469 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC220155 weakly similar to UP|FUT2_ARATH (O81053) Probable fucos... 306 1e-83
TC218856 weakly similar to UP|FUT4_ARATH (Q9SJP2) Probable fucos... 293 9e-80
BE823653 219 3e-57
TC222677 similar to UP|FUT1_PEA (Q9M5Q1) Galactoside 2-alpha-L-f... 197 1e-50
TC235117 107 1e-46
CD412213 weakly similar to SP|Q9M5Q1|FUT1_ Galactoside 2-alpha-L... 107 9e-34
AW508697 135 4e-32
AW704030 similar to SP|Q9M5Q1|FUT1 Galactoside 2-alpha-L-fucosyl... 119 2e-27
TC232667 homologue to UP|FUT1_PEA (Q9M5Q1) Galactoside 2-alpha-L... 115 4e-26
BG047146 homologue to SP|Q9M5Q1|FUT1_ Galactoside 2-alpha-L-fuco... 52 4e-07
BE474032 similar to SP|Q9M5Q1|FUT1_ Galactoside 2-alpha-L-fucosy... 42 8e-04
TC223203 similar to PIR|H88465|H88465 protein B0244.3 [imported]... 30 3.0
TC225018 homologue to UP|Q39865 (Q39865) Hydroxyproline-rich gly... 30 3.0
TC214588 homologue to UP|Q71SQ1 (Q71SQ1) MYC1, partial (35%) 29 5.2
TC205695 29 5.2
BF424530 weakly similar to GP|15451136|gb| periaxin-like protein... 28 8.8
BG881719 similar to SP|P59049|OMT1_ Quercetin 3-O-methyltransfer... 28 8.8
TC234692 weakly similar to UP|Q6Z5K3 (Q6Z5K3) Cellulose synthase... 28 8.8
>TC220155 weakly similar to UP|FUT2_ARATH (O81053) Probable
fucosyltransferase 2 (AtFUT2) , partial (22%)
Length = 1005
Score = 306 bits (785), Expect = 1e-83
Identities = 153/248 (61%), Positives = 187/248 (74%), Gaps = 6/248 (2%)
Frame = +1
Query: 228 MFPEITSTFHHLGRYLFHPSNEAWELITSFYQQNLAKANERIGLQIRVVDPKLTPHQVVM 287
MFPE FHHLGRYLFHPSN+AWELI+S+YQ +LA A+ERIGLQIRV + TP Q +M
Sbjct: 22 MFPERDIIFHHLGRYLFHPSNDAWELISSYYQAHLASASERIGLQIRVFNAATTPKQAIM 201
Query: 288 NQILNCTLGNKLLPKVLGTKNIYLSSSDKNKKIMKAVLVSSLYRHYGENLKMTYMNKSTV 347
N + CTL K+LP+V +SS+ KN +KAVLV+SL++ YG+NL+ Y+ K TV
Sbjct: 202 NLVFFCTLQQKILPEV--DLKTSVSSAGKNMTTVKAVLVASLHKEYGDNLRSMYLKKPTV 375
Query: 348 SGEVIEVYQPSGEEQQKFNDNKHNMKAWVDIYLLSLSDVLVTTYQSTFGYVAKALGNSRP 407
SGEVIEVYQPS E +QKFNDN HN+KAW D+YLLSLSDVLVTT STFGYVA+ LGN +P
Sbjct: 376 SGEVIEVYQPSHEGKQKFNDNGHNLKAWTDMYLLSLSDVLVTTSLSTFGYVAQGLGNLKP 555
Query: 408 WILYNPV--YSNEI--CEREFTLEPCYHYPPLHYCNGKPIEDVGSSFPYIRHCKDYTFGV 463
W+LY V +S + CER+F+ EPC+H PP HYCNG+P+ D+ SSFP +R CKD FGV
Sbjct: 556 WLLYRLVGNHSGDFPRCERDFSTEPCFHMPPKHYCNGEPMNDIVSSFPNLRECKDLRFGV 735
Query: 464 KL--VNAS 469
KL VN S
Sbjct: 736 KLAAVNGS 759
>TC218856 weakly similar to UP|FUT4_ARATH (Q9SJP2) Probable
fucosyltransferase 4 (AtFUT4) , partial (27%)
Length = 1581
Score = 293 bits (751), Expect = 9e-80
Identities = 144/275 (52%), Positives = 192/275 (69%), Gaps = 3/275 (1%)
Frame = +1
Query: 196 LLSNIPLLFLEAGQYFVPSFFMTPIFKKELNKMFPEITSTFHHLGRYLFHPSNEAWELIT 255
LL IP+L L + QYFVPS FM P F+++L+KMFPE + FHHLGRYL HPSNEAWE+I
Sbjct: 4 LLQRIPVLILWSDQYFVPSLFMIPSFRQDLSKMFPEKDTVFHHLGRYLLHPSNEAWEIIR 183
Query: 256 SFYQQNLAKANERIGLQIRVVDPKLTPHQVVMNQILNCTLGNKLLPKVLGTKNIYLSSSD 315
FY+ +LAKANERIGLQ+RV + PHQ ++N+I+ CTL +KLLP ++ S++
Sbjct: 184 KFYEAHLAKANERIGLQVRVFNTHRAPHQTIINEIIACTLQHKLLPDF----DMQKSATS 351
Query: 316 KNKKIMKAVLVSSLYRHYGENLKMTYMNKSTVSGEVIEVYQPSGEEQQKFNDNKHNMKAW 375
KK KAVLV+SL+ YG+ L+ Y +TV+ EVI VYQPS EE+QK N++ HN+KAW
Sbjct: 352 PLKKPSKAVLVASLFSEYGQKLRTMYQANTTVTREVIRVYQPSHEERQKSNNDMHNIKAW 531
Query: 376 VDIYLLSLSDVLVTTYQSTFGYVAKALGNSRPWIL---YNPVYSNEICEREFTLEPCYHY 432
+IYLLSL D LVT+ +STFGYVA +LG +PWIL Y + C R ++EPC+HY
Sbjct: 532 TEIYLLSLCDALVTSPKSTFGYVAHSLGGLKPWILQRAYGETIPDPPCRRAKSMEPCFHY 711
Query: 433 PPLHYCNGKPIEDVGSSFPYIRHCKDYTFGVKLVN 467
PP + C D S F +++HC+D + G++LVN
Sbjct: 712 PPKYDCRANSTVDFTSIFHHMKHCEDVSSGLRLVN 816
>BE823653
Length = 721
Score = 219 bits (557), Expect = 3e-57
Identities = 107/184 (58%), Positives = 136/184 (73%), Gaps = 4/184 (2%)
Frame = -2
Query: 290 ILNCTLGNKLLPKVLGTKNIYLSSSDKNKKIMKAVLVSSLYRHYGENLKMTYMNKSTVSG 349
+L+CTL +K+L V + K + +KAVLV+SLYR YG+ LK Y T+SG
Sbjct: 720 VLSCTLKHKILXXVX-----LQTXXXKXQTTVKAVLVASLYREYGDXLKRMYRKNPTLSG 556
Query: 350 EVIEVYQPSGEEQQKFNDNKHNMKAWVDIYLLSLSDVLVTTYQSTFGYVAKALGNSRPWI 409
EVI+VYQPS EE QK+NDNKHNMKAW+D+YLLSLSD LVTT STFGYVA+ LGN +PW+
Sbjct: 555 EVIKVYQPSHEEHQKYNDNKHNMKAWIDMYLLSLSDELVTTSLSTFGYVAQGLGNLKPWL 376
Query: 410 LYNPVYSNEI----CEREFTLEPCYHYPPLHYCNGKPIEDVGSSFPYIRHCKDYTFGVKL 465
LY V +NE CER+F+ EPCYH+PP HYCNG+P++D+ SSFPY+R CKD+ G+K+
Sbjct: 375 LYKLV-NNETHFPPCERDFSSEPCYHFPPKHYCNGEPLKDIVSSFPYLRPCKDFRVGLKM 199
Query: 466 VNAS 469
V S
Sbjct: 198 VKDS 187
>TC222677 similar to UP|FUT1_PEA (Q9M5Q1) Galactoside
2-alpha-L-fucosyltransferase (Xyloglucan
alpha-(1,2)-fucosyltransferase) (PsFT1) , partial (41%)
Length = 737
Score = 197 bits (500), Expect = 1e-50
Identities = 98/229 (42%), Positives = 143/229 (61%), Gaps = 5/229 (2%)
Frame = +1
Query: 80 ATTCKYIIWLPANGLGNQIISMASSFLYALLTGRVMLVQFGKDKEGLFCEPFLNSTWLLP 139
++ CKY++W+ +GLGN+I+++AS+FLYALLT RV+LV G D LFCEPF +S+WLLP
Sbjct: 28 SSECKYVVWISFSGLGNRILTLASAFLYALLTNRVLLVDPGADMVDLFCEPFPDSSWLLP 207
Query: 140 EKSPFWNAEKVQTYQSTIKMGGAN-----TLNEDFPSALHVNLGYSPTSEERFFHCDHNQ 194
P NA+ Q++ G + S +++++ + ++ F CD Q
Sbjct: 208 SDFPL-NAQFNNFSQNSDHCYGKMLKSKAVTDSTVASFVYLHIAHDYDDHDKLFFCDEEQ 384
Query: 195 FLLSNIPLLFLEAGQYFVPSFFMTPIFKKELNKMFPEITSTFHHLGRYLFHPSNEAWELI 254
L +P L ++ YFVPS F+ P F++ELN +FP + FH L RYLFHP+N W L+
Sbjct: 385 RFLQIVPWLIMKTDNYFVPSVFLMPSFEQELNDLFPNKETVFHFLSRYLFHPTNSVWGLV 564
Query: 255 TSFYQQNLAKANERIGLQIRVVDPKLTPHQVVMNQILNCTLGNKLLPKV 303
+YQ L+KA+ER+G+QIRV D + P Q V++QIL CTL +LP V
Sbjct: 565 VRYYQAYLSKADERVGIQIRVFDTEPGPFQHVLDQILACTLKKNILPDV 711
>TC235117
Length = 457
Score = 107 bits (267), Expect(2) = 1e-46
Identities = 51/71 (71%), Positives = 55/71 (76%)
Frame = +1
Query: 25 QSHFYHKPSPHKPSPYLISKLRKYEELHRRCGPNTRAYNEDMKKITKSKKNGTSAATTCK 84
+SH Y K SPHKPSPYLISKLRKYEE+HRRCGPNTR Y++ MKKI SK NG AAT CK
Sbjct: 22 ESHLYRKDSPHKPSPYLISKLRKYEEIHRRCGPNTRDYDKSMKKIVSSKNNG--AATKCK 195
Query: 85 YIIWLPANGLG 95
YIIW A G
Sbjct: 196YIIWNTAIWFG 228
Score = 98.2 bits (243), Expect(2) = 1e-46
Identities = 44/67 (65%), Positives = 56/67 (82%), Gaps = 2/67 (2%)
Frame = +2
Query: 93 GLGNQIISMASSFLYALLTGRVMLVQFGKDKEGLFCEPFLNSTWLLPEKSPF--WNAEKV 150
GLGNQ+IS+A++FLYA+LT R +LV+F KDK GLFCEPFLNSTW+LP+KSPF WN
Sbjct: 221 GLGNQMISIAATFLYAILTDRGLLVKFNKDKHGLFCEPFLNSTWILPQKSPFSSWNEHNT 400
Query: 151 QTYQSTI 157
+TYQ+ +
Sbjct: 401 ETYQTML 421
>CD412213 weakly similar to SP|Q9M5Q1|FUT1_ Galactoside
2-alpha-L-fucosyltransferase (EC 2.4.1.69) (Xyloglucan
alpha-(1 2)-fucosyltransferase), partial (14%)
Length = 573
Score = 107 bits (266), Expect(2) = 9e-34
Identities = 51/108 (47%), Positives = 69/108 (63%), Gaps = 3/108 (2%)
Frame = -2
Query: 363 QKFNDNKHNMKAWVDIYLLSLSDVLVTTYQSTFGYVAKALGNSRPWIL---YNPVYSNEI 419
Q ND+ HN+KAW +IYLLSL + LVT+ +STFGYVA +LG +PWIL Y +
Sbjct: 413 QNSNDDMHNIKAWTEIYLLSLCNALVTSPRSTFGYVAHSLGGLKPWILQGVYGKTILDPP 234
Query: 420 CEREFTLEPCYHYPPLHYCNGKPIEDVGSSFPYIRHCKDYTFGVKLVN 467
C+R +EPC+ YPP + C D S F +I+HC D + G++LVN
Sbjct: 233 CQRAKYMEPCFQYPPEYDCRANKTIDFPSIFNHIKHCDDVSSGLRLVN 90
Score = 55.1 bits (131), Expect(2) = 9e-34
Identities = 27/50 (54%), Positives = 34/50 (68%)
Frame = -1
Query: 312 SSSDKNKKIMKAVLVSSLYRHYGENLKMTYMNKSTVSGEVIEVYQPSGEE 361
++S K+ KAVLV SLY YGE L+ Y +TV+G+VI VYQPS EE
Sbjct: 570 ATSPLKKQTSKAVLVVSLYSEYGEKLRTMYQENTTVTGDVIRVYQPSHEE 421
>AW508697
Length = 448
Score = 135 bits (340), Expect = 4e-32
Identities = 63/91 (69%), Positives = 70/91 (76%)
Frame = +3
Query: 1 DEKEKLLDGLLVSGFDEASCVSRLQSHFYHKPSPHKPSPYLISKLRKYEELHRRCGPNTR 60
D+ +KLL GLL SGFDE SC+SR+QSH Y K SPHKPSPYLISKLR YEE+H RCGPNTR
Sbjct: 180 DDNDKLLGGLLTSGFDEESCISRIQSHLYRKASPHKPSPYLISKLRNYEEIHTRCGPNTR 359
Query: 61 AYNEDMKKITKSKKNGTSAATTCKYIIWLPA 91
AY+ M I SK G AAT CKY+IW PA
Sbjct: 360 AYHRSMTMIEHSKNKG--AATLCKYLIWTPA 446
>AW704030 similar to SP|Q9M5Q1|FUT1 Galactoside 2-alpha-L-fucosyltransferase
(EC 2.4.1.69) (Xyloglucan alpha-(1
2)-fucosyltransferase), partial (22%)
Length = 415
Score = 119 bits (299), Expect = 2e-27
Identities = 57/110 (51%), Positives = 77/110 (69%)
Frame = +2
Query: 2 EKEKLLDGLLVSGFDEASCVSRLQSHFYHKPSPHKPSPYLISKLRKYEELHRRCGPNTRA 61
+K+KLL GLL GFDE SC+SR S Y K PS YLIS+LRKYE LH++CGP T +
Sbjct: 92 QKDKLLGGLLADGFDEQSCLSRYHSATYSKGLSGNPSSYLISRLRKYEALHKKCGPYTES 271
Query: 62 YNEDMKKITKSKKNGTSAATTCKYIIWLPANGLGNQIISMASSFLYALLT 111
YN+ +K + + S + CKY++W+ +GLGN+I+++AS FLYALLT
Sbjct: 272 YNKTVKDL---RSGHVSESPECKYVVWISYSGLGNRILTLASVFLYALLT 412
>TC232667 homologue to UP|FUT1_PEA (Q9M5Q1) Galactoside
2-alpha-L-fucosyltransferase (Xyloglucan
alpha-(1,2)-fucosyltransferase) (PsFT1) , partial (19%)
Length = 468
Score = 115 bits (288), Expect = 4e-26
Identities = 50/103 (48%), Positives = 73/103 (70%), Gaps = 3/103 (2%)
Frame = +3
Query: 370 HNMKAWVDIYLLSLSDVLVTTYQSTFGYVAKALGNSRPWILYNP---VYSNEICEREFTL 426
HN KAW ++YLLSL+D+LVT+ STFGYVA+ LG +PWILY P + C+R ++
Sbjct: 30 HNQKAWAEMYLLSLTDMLVTSSWSTFGYVAQGLGGLKPWILYKPENGTAPDPPCQRAMSM 209
Query: 427 EPCYHYPPLHYCNGKPIEDVGSSFPYIRHCKDYTFGVKLVNAS 469
EPC+H PP + C K D G+ P++RHC+D ++G+KLV+++
Sbjct: 210 EPCFHAPPFYDCKAKRGTDTGALVPHVRHCEDMSWGLKLVDSN 338
>BG047146 homologue to SP|Q9M5Q1|FUT1_ Galactoside
2-alpha-L-fucosyltransferase (EC 2.4.1.69) (Xyloglucan
alpha-(1 2)-fucosyltransferase), partial (3%)
Length = 379
Score = 52.4 bits (124), Expect = 4e-07
Identities = 29/65 (44%), Positives = 41/65 (62%), Gaps = 2/65 (3%)
Frame = +2
Query: 2 EKEKLLDGLLVSGFD-EASCVSRLQ-SHFYHKPSPHKPSPYLISKLRKYEELHRRCGPNT 59
+++KLL GLL GFD E +C+SR Q + +YHK KP +LIS+L+K + CGP T
Sbjct: 86 QQDKLLGGLLADGFDDEETCLSRCQGAMYYHKWLSEKPYSHLISRLKKC*PQRKPCGPFT 265
Query: 60 RAYNE 64
N+
Sbjct: 266 ECSNK 280
>BE474032 similar to SP|Q9M5Q1|FUT1_ Galactoside 2-alpha-L-fucosyltransferase
(EC 2.4.1.69) (Xyloglucan alpha-(1
2)-fucosyltransferase), partial (10%)
Length = 418
Score = 41.6 bits (96), Expect = 8e-04
Identities = 20/39 (51%), Positives = 26/39 (66%)
Frame = +3
Query: 2 EKEKLLDGLLVSGFDEASCVSRLQSHFYHKPSPHKPSPY 40
+++KLL GLL GFDE SC+SR + +HK KPS Y
Sbjct: 309 QQDKLLGGLLADGFDEKSCLSR---YHFHKGLSEKPSSY 416
>TC223203 similar to PIR|H88465|H88465 protein B0244.3 [imported] -
Caenorhabditis elegans {Caenorhabditis elegans;} ,
partial (46%)
Length = 624
Score = 29.6 bits (65), Expect = 3.0
Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 3/41 (7%)
Frame = +2
Query: 192 HNQFLLSNIPLLFLEAGQYFVPSF---FMTPIFKKELNKMF 229
H L N PLL L +YF+P F F+ P K +L+++F
Sbjct: 2 HEALLHCNKPLLTLLKTRYFMPLFPLIFLFPFLKTDLHQVF 124
>TC225018 homologue to UP|Q39865 (Q39865) Hydroxyproline-rich glycoprotein
(Fragment), partial (19%)
Length = 434
Score = 29.6 bits (65), Expect = 3.0
Identities = 16/49 (32%), Positives = 23/49 (46%), Gaps = 2/49 (4%)
Frame = +3
Query: 27 HFYHKPSPHKPSPYL--ISKLRKYEELHRRCGPNTRAYNEDMKKITKSK 73
++YH P P P PY ++ L YEE +R + R + TK K
Sbjct: 66 YYYHSPPPPSPPPYHY*LA*LYGYEEYVQRIKESDRKGRRSASRCTKEK 212
>TC214588 homologue to UP|Q71SQ1 (Q71SQ1) MYC1, partial (35%)
Length = 837
Score = 28.9 bits (63), Expect = 5.2
Identities = 12/31 (38%), Positives = 18/31 (57%)
Frame = -1
Query: 12 VSGFDEASCVSRLQSHFYHKPSPHKPSPYLI 42
VSGF E +C +S H+PS H+ P ++
Sbjct: 345 VSGFVEVACYRPRKSRLLHQPSTHRFDPGIL 253
>TC205695
Length = 1332
Score = 28.9 bits (63), Expect = 5.2
Identities = 18/59 (30%), Positives = 27/59 (45%)
Frame = -2
Query: 129 EPFLNSTWLLPEKSPFWNAEKVQTYQSTIKMGGANTLNEDFPSALHVNLGYSPTSEERF 187
EP L S WL P WN+ + ++ ++ GG ED P N + T++ RF
Sbjct: 191 EPSLESPWLSPYCCC*WNSNPLH-LETALEFGGRGNKGEDTPEKERSN--QTRTTQTRF 24
>BF424530 weakly similar to GP|15451136|gb| periaxin-like protein
{Arabidopsis thaliana}, partial (12%)
Length = 386
Score = 28.1 bits (61), Expect = 8.8
Identities = 10/32 (31%), Positives = 19/32 (59%)
Frame = +2
Query: 408 WILYNPVYSNEICEREFTLEPCYHYPPLHYCN 439
W+L + ++SN+ C+ + C H+P L C+
Sbjct: 143 WLLEHAIFSNQPCQN----QKCQHFPNLMSCH 226
>BG881719 similar to SP|P59049|OMT1_ Quercetin 3-O-methyltransferase 1 (EC
2.1.1.76) (Flavonol 3-O- methyltransferase 1)., partial
(17%)
Length = 413
Score = 28.1 bits (61), Expect = 8.8
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = -1
Query: 214 SFFMTPIFKKELNKMFPEITSTFHHLGRYLFHPSN 248
+ F+TPI K L+K + +T H+ Y+F N
Sbjct: 365 ALFITPIVKYPLHKNGVSVGNTLKHISSYMFDTCN 261
>TC234692 weakly similar to UP|Q6Z5K3 (Q6Z5K3) Cellulose synthase-like
protein OsCslE2, partial (55%)
Length = 723
Score = 28.1 bits (61), Expect = 8.8
Identities = 23/97 (23%), Positives = 45/97 (45%), Gaps = 6/97 (6%)
Frame = +3
Query: 240 GRYLFHPSNEAWELI--TSFYQQNLAKANER----IGLQIRVVDPKLTPHQVVMNQILNC 293
G Y + W+++ T F + L + +E + + + DP L P +VMN +L+
Sbjct: 258 GIYWIITQSVRWKIVYQTPFKHRLLQRYDEESLPAVDIFVCTADPTLEPPCMVMNTVLSA 437
Query: 294 TLGNKLLPKVLGTKNIYLSSSDKNKKIMKAVLVSSLY 330
N K+ ++YLS ++ A+L +S++
Sbjct: 438 MAYNYPANKL----SVYLSDDGGSELTFYALLKASIF 536
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.136 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,466,085
Number of Sequences: 63676
Number of extensions: 417935
Number of successful extensions: 2401
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 2287
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2388
length of query: 469
length of database: 12,639,632
effective HSP length: 101
effective length of query: 368
effective length of database: 6,208,356
effective search space: 2284675008
effective search space used: 2284675008
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146553.5