BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146552.2 - phase: 0 /pseudo
(392 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC204391 homologue to UP|Q9ASR1 (Q9ASR1) At1g56070/T6H22_13 (Elo... 229 2e-60
TC230334 similar to UP|Q8RWP3 (Q8RWP3) GTP-binding protein LepA-... 104 8e-23
TC216233 GB|CAA50573.1|402753|GMFUSA translation elongation fact... 100 9e-22
TC220357 homologue to UP|Q6SYB1 (Q6SYB1) GTP-binding protein Typ... 97 1e-20
TC214026 similar to UP|Q6G0R7 (Q6G0R7) GTP-binding protein typA,... 81 9e-16
TC214405 homologue to UP|EFT1_SOYBN (Q43467) Elongation factor T... 70 1e-12
TC214319 homologue to UP|EFT1_SOYBN (Q43467) Elongation factor T... 70 2e-12
TC210299 homologue to UP|EFT2_SOYBN (P46280) Elongation factor T... 67 2e-11
TC217724 homologue to UP|EFTM_ARATH (Q9ZT91) Elongation factor T... 60 2e-09
TC232370 weakly similar to UP|Q9LS91 (Q9LS91) Elongation factor ... 45 7e-05
BF009693 similar to SP|P74751|LEPA_ GTP-binding protein lepA. [s... 44 1e-04
AW707054 similar to GP|15450763|gb| elongation factor EF-2 {Arab... 41 8e-04
TC220356 similar to UP|Q6SYB1 (Q6SYB1) GTP-binding protein TypA,... 40 0.002
BU926992 homologue to GP|11181616|gb| translational elongation f... 39 0.003
TC204393 homologue to UP|Q8RVT3 (Q8RVT3) Elongation factor EF-2 ... 39 0.005
BF068251 similar to GP|15450763|gb| elongation factor EF-2 {Arab... 35 0.045
TC233828 similar to PIR|T01917|T01917 translation initiation fac... 32 0.38
BM095025 32 0.65
TC206442 similar to GB|AAR04332.2|37813067|AY423375 eukaryotic t... 31 0.84
BI785638 similar to SP|O81481|IFE1_ Eukaryotic translation initi... 31 0.84
>TC204391 homologue to UP|Q9ASR1 (Q9ASR1) At1g56070/T6H22_13 (Elongation
factor EF-2), complete
Length = 2941
Score = 229 bits (584), Expect = 2e-60
Identities = 137/369 (37%), Positives = 198/369 (53%), Gaps = 16/369 (4%)
Frame = +1
Query: 11 RKKIRNICILAHVDHGKTTLADQLIAAASGGMVHPKVAGKVRFMDYLDEEQRRAITMKSS 70
+ IRN+ ++AHVDHGK+TL D L+AAA G++ +VAG VR D +E R IT+KS+
Sbjct: 166 KHNIRNMSVIAHVDHGKSTLTDSLVAAA--GIIAQEVAGDVRMTDTRADEAERGITIKST 339
Query: 71 SISLHY----------------NHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVE 114
ISL+Y N + +NLIDSPGH+DF EV+ A R++DGAL++VD VE
Sbjct: 340 GISLYYEMTDEALKSFKGERNGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVVDCVE 519
Query: 115 GVHIQTHAVLRQCWTEMLEPCLVLNKMDRLITELNLTPLEAYTRLLRIVHEVNGIWSAYN 174
GV +QT VLRQ E + P L +NKMDR EL + EAY R++ N I + Y
Sbjct: 520 GVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFQRVIENANVIMATY- 696
Query: 175 SEKYLSDVDALLAGGTAAGGEVMEDYDDVEDKFQPQKGNVVFACALDGWGFGIHEFAEIY 234
+ L DV + P+KG V F+ L GW F + FA++Y
Sbjct: 697 EDPLLGDV-----------------------QVYPEKGTVAFSAGLHGWAFTLTNFAKMY 807
Query: 235 ASKLGGSASVGALLRALWGPWYYNPKTKMIVGKKGISGSKARPMFVQFVLEPLWQVYQGA 294
ASK G G ++ LWG +++P TK K S + R FVQF EP+ Q+
Sbjct: 808 ASKFG--VDEGKMMERLWGENFFDPATKKWTSKNTGSATCKRG-FVQFCYEPIKQIINTC 978
Query: 295 LGGGKGMVEKVIKSFNLQIQARELQNKDSKVVLQAVMSRWLPLSDAILSMVLKCLPDPVE 354
+ K + +++ + +++ E ++ K +++ VM WLP S A+L M++ LP P
Sbjct: 979 MNDQKDKLWPMLQKLGVTMKSEE-KDLMGKALMKRVMQTWLPASSALLEMMIFHLPSPST 1155
Query: 355 GQKSRISRL 363
QK R+ L
Sbjct: 1156AQKYRVENL 1182
>TC230334 similar to UP|Q8RWP3 (Q8RWP3) GTP-binding protein LepA-like
protein, partial (27%)
Length = 820
Score = 104 bits (259), Expect = 8e-23
Identities = 60/135 (44%), Positives = 84/135 (61%), Gaps = 4/135 (2%)
Frame = +2
Query: 12 KKIRNICILAHVDHGKTTLADQLIAAASGGMVHPKVAGKVRFMDYLDEEQRRAITMKSSS 71
+ IRN CI+AH+DHGK+TLAD+L+ G VH + K +F+D +D E+ R IT+K +
Sbjct: 308 RNIRNFCIIAHIDHGKSTLADKLLQVT--GTVHQREM-KDQFLDNMDLERERGITIKLQA 478
Query: 72 ISLHY----NHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVEGVHIQTHAVLRQC 127
+ Y + +NLID+PGH+DF EVS + +GALL+VDA +GV QT A +
Sbjct: 479 ARMRYVFENEPYCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQTLANVYLA 658
Query: 128 WTEMLEPCLVLNKMD 142
LE VLNK+D
Sbjct: 659 LENNLEIIPVLNKID 703
>TC216233 GB|CAA50573.1|402753|GMFUSA translation elongation factor EF-G
{Glycine max;} , complete
Length = 2424
Score = 100 bits (250), Expect = 9e-22
Identities = 53/133 (39%), Positives = 79/133 (58%)
Frame = +1
Query: 12 KKIRNICILAHVDHGKTTLADQLIAAASGGMVHPKVAGKVRFMDYLDEEQRRAITMKSSS 71
K RNI I+AH+D GKTT ++++ +V MD++++EQ R IT+ S++
Sbjct: 40 KDYRNIGIMAHIDAGKTTTTERILYYTGRNYKIGEVHEGTATMDWMEQEQERGITITSAA 219
Query: 72 ISLHYNHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVEGVHIQTHAVLRQCWTEM 131
+ +N H +N+ID+PGH+DF EV A R+ DGA+ L D+V GV Q+ V RQ
Sbjct: 220 TTTFWNKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAGVEPQSETVWRQADKYG 399
Query: 132 LEPCLVLNKMDRL 144
+ +NKMDRL
Sbjct: 400 VPRICFVNKMDRL 438
>TC220357 homologue to UP|Q6SYB1 (Q6SYB1) GTP-binding protein TypA, partial
(25%)
Length = 668
Score = 97.1 bits (240), Expect = 1e-20
Identities = 57/150 (38%), Positives = 84/150 (56%), Gaps = 7/150 (4%)
Frame = +2
Query: 1 MEESTSDNNDRKK-------IRNICILAHVDHGKTTLADQLIAAASGGMVHPKVAGKVRF 53
+ E+T ++K+ +RNI I+AHVDHGKTTL D ++ + + R
Sbjct: 170 VSEATEPKTEKKRQLLRRGDVRNIAIVAHVDHGKTTLVDAMLKQTK--VFRDNQFVQERI 343
Query: 54 MDYLDEEQRRAITMKSSSISLHYNHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAV 113
MD D E+ R IT+ S + S+ Y +N+ID+PGH DF GEV + +G LL+VD+V
Sbjct: 344 MDSNDLERERGITILSKNTSVTYKDAKINIIDTPGHSDFGGEVERILNMVEGILLVVDSV 523
Query: 114 EGVHIQTHAVLRQCWTEMLEPCLVLNKMDR 143
EG QT VL++ +V+NK+DR
Sbjct: 524 EGPMPQTRFVLKKALEFGHSVVVVVNKIDR 613
>TC214026 similar to UP|Q6G0R7 (Q6G0R7) GTP-binding protein typA, partial
(14%)
Length = 501
Score = 80.9 bits (198), Expect = 9e-16
Identities = 38/104 (36%), Positives = 62/104 (59%)
Frame = +1
Query: 4 STSDNNDRKKIRNICILAHVDHGKTTLADQLIAAASGGMVHPKVAGKVRFMDYLDEEQRR 63
+T+ + D ++RN+ ++AHVDHGKTTL D+L+ + H R MD + E+ R
Sbjct: 208 TTASSLDPSRLRNLAVIAHVDHGKTTLMDRLLRQCGADLPHE------RAMDSISLERER 369
Query: 64 AITMKSSSISLHYNHHTVNLIDSPGHIDFCGEVSTAARLSDGAL 107
IT+ S S+ + + +N++D+PGH DF GEV + +GA+
Sbjct: 370 GITISSKVTSVSWKENELNMVDTPGHADFGGEVERVVGMVEGAI 501
>TC214405 homologue to UP|EFT1_SOYBN (Q43467) Elongation factor Tu,
chloroplast precursor (EF-Tu), complete
Length = 2050
Score = 70.5 bits (171), Expect = 1e-12
Identities = 66/208 (31%), Positives = 101/208 (47%), Gaps = 15/208 (7%)
Frame = +3
Query: 10 DRKKIR-NICILAHVDHGKTTLADQL-IAAASGGMVHPKVAGKVRFMDYLDEEQRRAITM 67
+RKK NI + HVDHGKTTL L +A A+ G PK K +D EE+ R IT+
Sbjct: 252 ERKKPHVNIGTIGHVDHGKTTLTAALTMALAALGNSAPK---KYDEIDAAPEERARGITI 422
Query: 68 KSSSISLHYNHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVEGVHIQT--HAVLR 125
++++ + +D PGH D+ + T A DGA+L+V +G QT H +L
Sbjct: 423 NTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLA 602
Query: 126 QCWTEMLEPCLV--LNKMDRLITELNLTPLEAYTRLLRIVHEVNGIWSAYNSEKYLSDVD 183
+ ++ P +V LNK D++ E L +E R L +E G + S L ++
Sbjct: 603 K---QVGVPNMVVFLNKQDQVDDEELLQLVELEVRDLLTSYEFPGDDTPIVSGSALLALE 773
Query: 184 ALLAGGTAAGGE---------VMEDYDD 202
AL+A G+ +M++ DD
Sbjct: 774 ALMANPAIKRGDNEWVDKIYKLMDEVDD 857
>TC214319 homologue to UP|EFT1_SOYBN (Q43467) Elongation factor Tu,
chloroplast precursor (EF-Tu), complete
Length = 1583
Score = 69.7 bits (169), Expect = 2e-12
Identities = 63/192 (32%), Positives = 95/192 (48%), Gaps = 6/192 (3%)
Frame = +1
Query: 10 DRKKIR-NICILAHVDHGKTTLADQL-IAAASGGMVHPKVAGKVRFMDYLDEEQRRAITM 67
+RKK NI + HVDHGKTTL L +A A+ G PK K +D EE+ R IT+
Sbjct: 229 ERKKPHVNIGTIGHVDHGKTTLTAALTMALAALGNSAPK---KYDEIDAAPEERARGITI 399
Query: 68 KSSSISLHYNHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVEGVHIQT--HAVLR 125
++++ + +D PGH D+ + T A DGA+L+V +G QT H +L
Sbjct: 400 NTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLA 579
Query: 126 QCWTEMLEPCLV--LNKMDRLITELNLTPLEAYTRLLRIVHEVNGIWSAYNSEKYLSDVD 183
+ ++ P +V LNK D++ E L +E R L +E G + S L ++
Sbjct: 580 K---QVGVPNMVVFLNKQDQVDDEELLQLVELEVRDLLSSYEFPGDDTPIVSGSALLALE 750
Query: 184 ALLAGGTAAGGE 195
AL+A G+
Sbjct: 751 ALMANPAIKRGD 786
>TC210299 homologue to UP|EFT2_SOYBN (P46280) Elongation factor Tu,
chloroplast precursor (EF-Tu), complete
Length = 1615
Score = 66.6 bits (161), Expect = 2e-11
Identities = 56/153 (36%), Positives = 80/153 (51%), Gaps = 8/153 (5%)
Frame = +1
Query: 10 DRKKIR-NICILAHVDHGKTTLADQL-IAAASGGMVHPKVAGKVRFMDYLDEEQRRAITM 67
+RKK NI + HVDHGKTTL L +A AS G PK K +D EE+ R IT+
Sbjct: 229 ERKKPHVNIGTIGHVDHGKTTLTAALTMALASLGNSAPK---KYDEIDAAPEERARGITI 399
Query: 68 KSSSISLHYNHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVEGVHIQT--HAVLR 125
++++ + +D PGH D+ + T A DGA+L+V +G QT H +L
Sbjct: 400 NTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLA 579
Query: 126 QCWTEMLEPCLV--LNKMDRLITE--LNLTPLE 154
+ ++ P +V LNK D++ E L L LE
Sbjct: 580 K---QVGVPNIVVFLNKQDQVDDEELLQLVELE 669
>TC217724 homologue to UP|EFTM_ARATH (Q9ZT91) Elongation factor Tu,
mitochondrial precursor, partial (87%)
Length = 1679
Score = 59.7 bits (143), Expect = 2e-09
Identities = 48/139 (34%), Positives = 71/139 (50%), Gaps = 12/139 (8%)
Frame = +2
Query: 16 NICILAHVDHGKTTLADQLIAAASGGMVHPKVAGKVRFMDYLD---EEQRRAITMKSSSI 72
N+ + HVDHGKTTL AA ++ +V K D +D EE++R IT+ ++ +
Sbjct: 308 NVGTIGHVDHGKTTL-----TAAITRVLADEVKAKAVAFDEIDKAPEEKKRGITIATAHV 472
Query: 73 SL-----HYNHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVEGVHIQT--HAVLR 125
HY H +D PGH D+ + T A DG +L+V A +G QT H +L
Sbjct: 473 EYETAKRHYAH-----VDCPGHADYVKNMITGAAQMDGGILVVSAPDGPMPQTKEHILLA 637
Query: 126 QCWTEMLEPCLV--LNKMD 142
+ ++ P LV LNK+D
Sbjct: 638 R---QVGVPSLVCFLNKVD 685
>TC232370 weakly similar to UP|Q9LS91 (Q9LS91) Elongation factor EF-2,
partial (3%)
Length = 455
Score = 44.7 bits (104), Expect = 7e-05
Identities = 21/30 (70%), Positives = 24/30 (80%)
Frame = -2
Query: 87 PGHIDFCGEVSTAARLSDGALLLVDAVEGV 116
P HIDF EVSTAARL+D LLLV+ VEG+
Sbjct: 157 PDHIDFYNEVSTAARLNDDVLLLVNVVEGI 68
>BF009693 similar to SP|P74751|LEPA_ GTP-binding protein lepA. [strain PCC
6803] {Synechocystis sp.}, partial (10%)
Length = 187
Score = 43.9 bits (102), Expect = 1e-04
Identities = 24/56 (42%), Positives = 35/56 (61%)
Frame = +2
Query: 12 KKIRNICILAHVDHGKTTLADQLIAAASGGMVHPKVAGKVRFMDYLDEEQRRAITM 67
+ IRN CI+AH+DHGK+TLA L+ G VH + K + D +D E+ R +T+
Sbjct: 8 RNIRNWCIIAHMDHGKSTLACNLLEVT--GTVHQREM-KDHWRDNMDLERERGMTI 166
>AW707054 similar to GP|15450763|gb| elongation factor EF-2 {Arabidopsis
thaliana}, partial (7%)
Length = 193
Score = 41.2 bits (95), Expect = 8e-04
Identities = 19/42 (45%), Positives = 29/42 (68%)
Frame = +2
Query: 14 IRNICILAHVDHGKTTLADQLIAAASGGMVHPKVAGKVRFMD 55
IRN+ ++AHVDH K+TL D L+AAA G++ ++ +R D
Sbjct: 53 IRNMSVIAHVDHRKSTLTDSLVAAA--GIIAQEITENMRMTD 172
>TC220356 similar to UP|Q6SYB1 (Q6SYB1) GTP-binding protein TypA, partial
(9%)
Length = 432
Score = 39.7 bits (91), Expect = 0.002
Identities = 18/42 (42%), Positives = 27/42 (63%), Gaps = 7/42 (16%)
Frame = +3
Query: 1 MEESTSDNNDRKK-------IRNICILAHVDHGKTTLADQLI 35
+ E+T ++K+ +RNI I+AHVDHGKTTL D ++
Sbjct: 261 VSEATEPKTEKKRQLLRRGDVRNIAIVAHVDHGKTTLVDAML 386
>BU926992 homologue to GP|11181616|gb| translational elongation factor EF-TuM
{Zea mays}, partial (20%)
Length = 444
Score = 39.3 bits (90), Expect = 0.003
Identities = 28/81 (34%), Positives = 40/81 (48%), Gaps = 7/81 (8%)
Frame = +1
Query: 16 NICILAHVDHGKTTLADQLIAAASGGMVHPKVAGKVRF--MDYLDEEQRRAITMKSSSIS 73
N+ + HVDHGKTTL AA + + A V F +D EE++R IT+ ++ +
Sbjct: 226 NVGTIGHVDHGKTTLT----AAITKVLADEGKAKAVAFDEIDKAPEEKKRGITIATAHVE 393
Query: 74 L-----HYNHHTVNLIDSPGH 89
HY H +D PGH
Sbjct: 394 YETAKRHYAH-----VDCPGH 441
>TC204393 homologue to UP|Q8RVT3 (Q8RVT3) Elongation factor EF-2 (Fragment),
partial (33%)
Length = 487
Score = 38.5 bits (88), Expect = 0.005
Identities = 17/40 (42%), Positives = 24/40 (59%)
Frame = +1
Query: 324 KVVLQAVMSRWLPLSDAILSMVLKCLPDPVEGQKSRISRL 363
K +++ VM WLP S A+L M++ LP P QK R+ L
Sbjct: 46 KALMKRVMQTWLPASSALLEMMIFHLPSPSTAQKYRVENL 165
>BF068251 similar to GP|15450763|gb| elongation factor EF-2 {Arabidopsis
thaliana}, partial (5%)
Length = 139
Score = 35.4 bits (80), Expect = 0.045
Identities = 13/24 (54%), Positives = 20/24 (83%)
Frame = +2
Query: 15 RNICILAHVDHGKTTLADQLIAAA 38
+N+ ++AHVDH K+TL D ++AAA
Sbjct: 50 QNVSVIAHVDHRKSTLTDSIVAAA 121
>TC233828 similar to PIR|T01917|T01917 translation initiation factor IF-2
homolog F2P3.9 - Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (23%)
Length = 459
Score = 32.3 bits (72), Expect = 0.38
Identities = 17/64 (26%), Positives = 32/64 (49%)
Frame = +2
Query: 80 TVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVEGVHIQTHAVLRQCWTEMLEPCLVLN 139
++ +D+PGH F + A ++D +L+V A +GV QT + + + +N
Sbjct: 29 SITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMSHAKAANVPIVVAIN 208
Query: 140 KMDR 143
K D+
Sbjct: 209 KCDK 220
>BM095025
Length = 335
Score = 31.6 bits (70), Expect = 0.65
Identities = 14/39 (35%), Positives = 21/39 (52%), Gaps = 7/39 (17%)
Frame = +2
Query: 334 WLPLSDAILSMVLKCLP-------DPVEGQKSRISRLIP 365
W+P A+L V++C+P DP G S + RL+P
Sbjct: 68 WVPRGHAVLRAVIRCVPRTEASHVDPSVGDVSELDRLVP 184
>TC206442 similar to GB|AAR04332.2|37813067|AY423375 eukaryotic translation
initiation factor 4E {Pisum sativum;} , partial (78%)
Length = 982
Score = 31.2 bits (69), Expect = 0.84
Identities = 13/40 (32%), Positives = 20/40 (49%)
Frame = +1
Query: 252 WGPWYYNPKTKMIVGKKGISGSKARPMFVQFVLEPLWQVY 291
W W+ NP +K K+ GS RP++ +E W +Y
Sbjct: 262 WTFWFDNPSSK---SKQAAWGSSIRPIYTFATVEEFWSIY 372
>BI785638 similar to SP|O81481|IFE1_ Eukaryotic translation initiation factor
4E (EIF-4E) (EIF4E) (mRNA cap-binding protein), partial
(34%)
Length = 433
Score = 31.2 bits (69), Expect = 0.84
Identities = 13/40 (32%), Positives = 20/40 (49%)
Frame = +1
Query: 252 WGPWYYNPKTKMIVGKKGISGSKARPMFVQFVLEPLWQVY 291
W W+ NP +K K+ GS RP++ +E W +Y
Sbjct: 223 WTFWFDNPSSK---SKQAAWGSSIRPIYTFATVEEFWSIY 333
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.136 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,086,168
Number of Sequences: 63676
Number of extensions: 204762
Number of successful extensions: 1053
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 1036
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1041
length of query: 392
length of database: 12,639,632
effective HSP length: 99
effective length of query: 293
effective length of database: 6,335,708
effective search space: 1856362444
effective search space used: 1856362444
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146552.2