BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146549.14 + phase: 0 /pseudo/partial
(221 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC209363 similar to UP|Q6NLD7 (Q6NLD7) At1g64640, partial (46%) 34 0.059
TC229078 similar to UP|O82458 (O82458) Rac GTPase activating pro... 33 0.10
TC224628 similar to UP|Q9ZST2 (Q9ZST2) Pyrophosphate-dependent p... 32 0.29
TC221191 similar to UP|O04862 (O04862) Folate synthesis protein ... 30 0.86
TC229535 homologue to UP|Q8LK24 (Q8LK24) SOS2-like protein kinas... 30 1.1
TC224758 similar to UP|PFPA_RICCO (Q41140) Pyrophosphate--fructo... 30 1.1
BE023588 28 2.5
BF596932 28 2.5
TC231062 weakly similar to UP|Q6N8I3 (Q6N8I3) CycJ cytochrome-c ... 27 5.5
TC210711 weakly similar to UP|Q6NLG7 (Q6NLG7) At4g18590, partial... 27 5.5
TC208881 UP|P93159 (P93159) Beta-glucan-elicitor receptor, complete 27 7.2
CO982496 27 9.5
TC214023 similar to GB|BAA23769.1|2668492|D89981 metal-transport... 27 9.5
TC220069 similar to UP|Q9SSY8 (Q9SSY8) Hsr203J homolog, partial ... 27 9.5
TC214393 similar to UP|Q7Y1T5 (Q7Y1T5) Oxygen-evolving enhancer ... 27 9.5
TC215348 similar to PIR|T00795|T00795 26S proteasome regulatory ... 27 9.5
>TC209363 similar to UP|Q6NLD7 (Q6NLD7) At1g64640, partial (46%)
Length = 1016
Score = 33.9 bits (76), Expect = 0.059
Identities = 20/84 (23%), Positives = 41/84 (48%)
Frame = +3
Query: 77 LLRAHRRGMVMDTTRRKKLKWGWYQLEPVNQWLLLLLSMQLKCLRHTHICRILSIRSSHR 136
+L ++ G + + + + P LL + S + +CL HTHI + S+ H+
Sbjct: 48 ILNRNQDGQFFKSPQNVGVSLAYVDPNPSQGVLLPIQSGRSRCLGHTHISKSTSL---HK 218
Query: 137 FIISTLCHRANLRFLSMPSLNRYN 160
+ + H L F+S+P+ +R++
Sbjct: 219 MVQIS*SHNWRLPFISIPTKSRFS 290
>TC229078 similar to UP|O82458 (O82458) Rac GTPase activating protein 1,
partial (70%)
Length = 1579
Score = 33.1 bits (74), Expect = 0.10
Identities = 17/54 (31%), Positives = 31/54 (56%), Gaps = 1/54 (1%)
Frame = -1
Query: 98 GWYQLEPVNQWLLLLLSMQLKCLRHTHICRI-LSIRSSHRFIISTLCHRANLRF 150
G+++L ++ L+ ++S QLK + C I L + S ++ + C RANLR+
Sbjct: 1087 GYWKLNHLSGTLVRVISAQLKAFAPANFCLIPLCLPGSEATVLKSNCQRANLRY 926
>TC224628 similar to UP|Q9ZST2 (Q9ZST2) Pyrophosphate-dependent
phosphofructokinase alpha subunit , complete
Length = 2315
Score = 31.6 bits (70), Expect = 0.29
Identities = 14/30 (46%), Positives = 19/30 (62%)
Frame = -2
Query: 90 TRRKKLKWGWYQLEPVNQWLLLLLSMQLKC 119
TR KLKW W ++ +++ L LLS QL C
Sbjct: 1777 TRTIKLKWTWISVDIIHKKALCLLSQQLIC 1688
>TC221191 similar to UP|O04862 (O04862) Folate synthesis protein precursor
[Includes:
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
pyrophosphokinase (HPPK); dihydropteroate synthase
(DHPS)] , partial (15%)
Length = 571
Score = 30.0 bits (66), Expect = 0.86
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = +3
Query: 144 HRANLRFLSMPSLNRYNINCRFNNNNKISKLGLLSLRYRCYMLSC 188
H N L P N FNN++ I + +LS+ Y + L+C
Sbjct: 360 HM*NTTMLPKPEQRSIKYNVAFNNHHTIVRFSILSILYLFHSLTC 494
>TC229535 homologue to UP|Q8LK24 (Q8LK24) SOS2-like protein kinase, partial
(34%)
Length = 969
Score = 29.6 bits (65), Expect = 1.1
Identities = 19/63 (30%), Positives = 29/63 (45%)
Frame = -2
Query: 47 PRTTFQRWSLWEPVLRKPSGMESLCLRKLNLLRAHRRGMVMDTTRRKKLKWGWYQLEPVN 106
P +T RW P+LR + S+ L + L+ H +V ++ L G+ QL
Sbjct: 194 PSSTCFRWQFLPPILRLAWPLNSVFLSRKPLISIH--SLVQPGPKQTTLPVGFSQLLNRT 21
Query: 107 QWL 109
QWL
Sbjct: 20 QWL 12
>TC224758 similar to UP|PFPA_RICCO (Q41140) Pyrophosphate--fructose
6-phosphate 1-phosphotransferase alpha subunit (PFP)
(6-phosphofructokinase, pyrophosphate dependent)
(Pyrophosphate-dependent 6-phosphofructose-1-kinase)
(PPi-PFK) , partial (29%)
Length = 1043
Score = 29.6 bits (65), Expect = 1.1
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = -2
Query: 94 KLKWGWYQLEPVNQWLLLLLSMQLKC 119
KLKW W ++ +++ L LLS QL C
Sbjct: 382 KLKWTWISVDVIHKKALCLLSQQLIC 305
>BE023588
Length = 424
Score = 28.5 bits (62), Expect = 2.5
Identities = 19/60 (31%), Positives = 30/60 (49%), Gaps = 6/60 (10%)
Frame = -3
Query: 109 LLLLLSMQLKCLRHTHICRILSIRSSHRFIISTLCHRANLR------FLSMPSLNRYNIN 162
+ LLL+ K +++ CR S++SS I LCH +NL L P+L N++
Sbjct: 305 ITLLLNHPQKLIQNCLGCRCSSLKSSSSKISIRLCHSSNLHDSHKTIELQFPTLGLNNLS 126
>BF596932
Length = 420
Score = 28.5 bits (62), Expect = 2.5
Identities = 19/62 (30%), Positives = 32/62 (50%), Gaps = 2/62 (3%)
Frame = -3
Query: 151 LSMPSLNRYNINCRFN--NNNKISKLGLLSLRYRCYMLSCFQLYSREGIVQLDRVSPHLI 208
+++ LN Y+ +C + ++LG L + ++SC YSREG+ +S HL
Sbjct: 211 IALKKLNTYSFSCWSK*ISACACNRLGKLPTKESRTLVSCT*RYSREGVTTCTLLS*HL- 35
Query: 209 RC 210
RC
Sbjct: 34 RC 29
>TC231062 weakly similar to UP|Q6N8I3 (Q6N8I3) CycJ cytochrome-c biosynthesis
protein precursor, partial (41%)
Length = 837
Score = 27.3 bits (59), Expect = 5.5
Identities = 20/56 (35%), Positives = 27/56 (47%), Gaps = 1/56 (1%)
Frame = +3
Query: 45 SLPRTTFQRWSLWEPVLRKPSGMESLCLRK-LNLLRAHRRGMVMDTTRRKKLKWGW 99
+ PR + +++ P PS M S+CLRK L LLR RR + M W W
Sbjct: 411 TFPRRRGR*SAIFRPRRFSPSMMRSICLRKLLLLLRRIRRLLRMLPLPPPPPPWPW 578
>TC210711 weakly similar to UP|Q6NLG7 (Q6NLG7) At4g18590, partial (62%)
Length = 606
Score = 27.3 bits (59), Expect = 5.5
Identities = 17/40 (42%), Positives = 20/40 (49%)
Frame = +3
Query: 154 PSLNRYNINCRFNNNNKISKLGLLSLRYRCYMLSCFQLYS 193
P +R ++ CR N I LSL Y C CFQLYS
Sbjct: 468 PQRSRAHVLCR---NCFIISYVRLSLAYVCI*FHCFQLYS 578
>TC208881 UP|P93159 (P93159) Beta-glucan-elicitor receptor, complete
Length = 2267
Score = 26.9 bits (58), Expect = 7.2
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -1
Query: 168 NNKISKLGLLSLRYRCYMLSCFQLYSREGIVQLD 201
N+ + ++ + Y Y C + Y R+G+VQLD
Sbjct: 698 NSLVFRIRIRQQSYPDYARKCIRSYLRKGVVQLD 597
>CO982496
Length = 596
Score = 26.6 bits (57), Expect = 9.5
Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 3/29 (10%)
Frame = -2
Query: 49 TTFQRWSLWEPVLRKPSGM---ESLCLRK 74
T QRW W +L KP GM ++ C++K
Sbjct: 445 TLDQRWWRWRFLLHKPGGM*KQKTTCMKK 359
>TC214023 similar to GB|BAA23769.1|2668492|D89981 metal-transporting P-type
ATPase {Arabidopsis thaliana;} , partial (21%)
Length = 828
Score = 26.6 bits (57), Expect = 9.5
Identities = 19/56 (33%), Positives = 27/56 (47%), Gaps = 14/56 (25%)
Frame = +3
Query: 107 QWLLLLLSMQLKCLRHTHI------------CRIL--SIRSSHRFIISTLCHRANL 148
QWL++ L+MQL L H + C +L SSHR ++ TL +NL
Sbjct: 522 QWLVMELTMQLPWLLHMLVLH*VEVSGLLVRCLLLY*CATSSHRSLLFTLIFSSNL 689
>TC220069 similar to UP|Q9SSY8 (Q9SSY8) Hsr203J homolog, partial (88%)
Length = 1071
Score = 26.6 bits (57), Expect = 9.5
Identities = 20/60 (33%), Positives = 29/60 (48%)
Frame = +2
Query: 52 QRWSLWEPVLRKPSGMESLCLRKLNLLRAHRRGMVMDTTRRKKLKWGWYQLEPVNQWLLL 111
QRWS +P R+P+ M S L K L+ + KL+W W Q+ + + LLL
Sbjct: 779 QRWSTTKP*-RRPTKMWSCLLTKERLI----------VSISTKLRWIWTQILVLKRRLLL 925
>TC214393 similar to UP|Q7Y1T5 (Q7Y1T5) Oxygen-evolving enhancer protein 3,
partial (55%)
Length = 1709
Score = 26.6 bits (57), Expect = 9.5
Identities = 19/71 (26%), Positives = 35/71 (48%), Gaps = 10/71 (14%)
Frame = -1
Query: 131 IRSSHRFIISTLCH-------RANLRFLSMPSLNRYNINCRFNNNNKISKLGLLSLRY-- 181
IR+ H +++++ + R+ F S+ S R+ +CR + KI L+ L+Y
Sbjct: 1307 IRARHHPLLASISYWHSH*NCRSGSHFFSVVS--RHEASCRIRHEEKIFPHHLIFLKYQS 1134
Query: 182 -RCYMLSCFQL 191
+C SC +L
Sbjct: 1133 IKCLRKSCTKL 1101
>TC215348 similar to PIR|T00795|T00795 26S proteasome regulatory subunit
[imported] - Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (34%)
Length = 1564
Score = 26.6 bits (57), Expect = 9.5
Identities = 22/77 (28%), Positives = 32/77 (40%), Gaps = 7/77 (9%)
Frame = +1
Query: 143 CHRANLRFLSMPSLNRY-------NINCRFNNNNKISKLGLLSLRYRCYMLSCFQLYSRE 195
CH +L FL+ PSL + N F KLGL L+ R ML L +
Sbjct: 529 CHMQSLHFLNTPSLLLFPPLLQP*NFPLLFCQPQLRLKLGLRKLKNRRLMLKFHLLPTLH 708
Query: 196 GIVQLDRVSPHLIRCLQ 212
+ Q+ L+R ++
Sbjct: 709 QLSQVVEKESRLVRRME 759
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.340 0.147 0.491
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,839,722
Number of Sequences: 63676
Number of extensions: 223259
Number of successful extensions: 2078
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 2069
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2078
length of query: 221
length of database: 12,639,632
effective HSP length: 94
effective length of query: 127
effective length of database: 6,654,088
effective search space: 845069176
effective search space used: 845069176
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146549.14