BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146548.14 - phase: 0
(180 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CD399491 166 7e-42
TC207846 similar to UP|Q9STU0 (Q9STU0) ABC-type transport-like p... 110 4e-25
BM309608 74 5e-14
TC218437 similar to UP|Q84M24 (Q84M24) AtABCA1, partial (12%) 64 5e-11
BQ273466 similar to GP|10176867|dbj contains similarity to ABC t... 59 2e-09
TC234765 similar to UP|Q84M24 (Q84M24) AtABCA1, partial (9%) 57 4e-09
AI988351 similar to GP|10176867|dbj contains similarity to ABC t... 57 5e-09
CF808489 55 2e-08
TC210950 similar to UP|Q9FMU0 (Q9FMU0) Similarity to ABC transpo... 48 3e-06
CD403377 47 5e-06
TC218418 homologue to UP|Q6X4V5 (Q6X4V5) ABC transporter, partia... 46 1e-05
TC222300 similar to GB|AAP37786.1|30725528|BT008427 At1g51560 {A... 45 2e-05
TC215951 similar to UP|Q9SXT3 (Q9SXT3) Multidrug resistance prot... 44 4e-05
TC207756 similar to UP|Q8RY46 (Q8RY46) Transporter associated wi... 44 5e-05
CA820193 similar to PIR|T04229|T04 ABC-type transport protein F1... 43 9e-05
TC208899 similar to UP|Q9LHD1 (Q9LHD1) Multidrug resistance p-gl... 42 2e-04
BI699796 similar to PIR|G85167|G85 ABC transporter like protein ... 42 2e-04
TC208020 similar to UP|Q76CU2 (Q76CU2) PDR-type ABC transporter ... 42 2e-04
TC207596 similar to UP|Q7GBY1 (Q7GBY1) Tonoplast ABC transporter... 42 2e-04
AW317336 similar to GP|4586576|dbj| multidrug resistance protein... 40 8e-04
>CD399491
Length = 635
Score = 166 bits (419), Expect = 7e-42
Identities = 79/95 (83%), Positives = 89/95 (93%)
Frame = -2
Query: 86 YGGTYVFAMTTSSDHEKDVENMVQRLTPNANKIYHLSGTQKFELPKEEIKMANVFRAIEA 145
YGGTYVF MTTSSDHEKDVE MVQ+LTPNANKIYHLSGTQKFELPK ++++ +VF+A++A
Sbjct: 634 YGGTYVFTMTTSSDHEKDVEYMVQKLTPNANKIYHLSGTQKFELPKGDVRITDVFQAVDA 455
Query: 146 AKRNFTVFAWGLSDTTLEDVFIKVAREAQAFDTLT 180
AKRNFTV AWGL DTTLEDVFIKVAREAQAFDTL+
Sbjct: 454 AKRNFTVSAWGLVDTTLEDVFIKVAREAQAFDTLS 350
>TC207846 similar to UP|Q9STU0 (Q9STU0) ABC-type transport-like protein,
partial (22%)
Length = 1078
Score = 110 bits (275), Expect = 4e-25
Identities = 65/175 (37%), Positives = 101/175 (57%), Gaps = 15/175 (8%)
Frame = +1
Query: 15 PKVVYMDEPSTGLDPASRKCLWNVIKLAKQDRAIILTTHSMEEADALCDRLGIFVDGSLQ 74
PK+V +DEP+TG+DP +R+ +W++I+ AK+ RAI+LTTHSMEEAD L DR+GI GSL+
Sbjct: 1 PKLVILDEPTTGMDPITRRHVWDIIENAKRGRAIVLTTHSMEEADILSDRIGIMAKGSLR 180
Query: 75 CVGYPKELKGRYGGTYVFAMT---TSSDHEKDVENMVQRLTPNANKIY---HLSGTQK-- 126
C+G LK R+G ++ ++ + +H + + A K + HL K
Sbjct: 181 CIGTSIRLKSRFGTGFIANISFNGNNIEHSPANGDAISTERREAVKKFFKNHLDVVPKEE 360
Query: 127 ------FELPKE-EIKMANVFRAIEAAKRNFTVFAWGLSDTTLEDVFIKVAREAQ 174
F +P + E M N F ++ + F + L TTLE+VF+ +AR+A+
Sbjct: 361 NHNFLTFVIPHDREALMTNFFSELQDREEEFGISDIQLGLTTLEEVFLNIARQAE 525
>BM309608
Length = 432
Score = 73.6 bits (179), Expect = 5e-14
Identities = 48/141 (34%), Positives = 69/141 (48%), Gaps = 16/141 (11%)
Frame = +2
Query: 46 RAIILTTHSMEEADALCDRLGIFVDGSLQCVGYPKELKGRYGGTYVFAMTTSSDHEKDVE 105
R I+LTTHSM+EAD L DR+ I +GSL+C G LK YG Y + S+
Sbjct: 8 RIILLTTHSMDEADELGDRIAIMANGSLKCCGSSLFLKHHYGVGYTLTLVKSAPTASIAG 187
Query: 106 NMVQRLTPNANKIYHLSGTQKFELP-KEEIKMANVFRAIEAA---------------KRN 149
++V R P+A + + F LP +FR IE K +
Sbjct: 188 DIVYRHVPSATCVSEVGTEISFRLPMASSSAFERMFREIEGCMKKTVSNMELSGNGDKDS 367
Query: 150 FTVFAWGLSDTTLEDVFIKVA 170
+ ++G+S TTLE+VF++VA
Sbjct: 368 LGIESYGISVTTLEEVFLRVA 430
>TC218437 similar to UP|Q84M24 (Q84M24) AtABCA1, partial (12%)
Length = 1235
Score = 63.5 bits (153), Expect = 5e-11
Identities = 29/65 (44%), Positives = 41/65 (62%)
Frame = +2
Query: 47 AIILTTHSMEEADALCDRLGIFVDGSLQCVGYPKELKGRYGGTYVFAMTTSSDHEKDVEN 106
A+ILTTHSM EA ALC R+GI V G L+C+G P+ LK R+G + + D++N
Sbjct: 5 AVILTTHSMNEAQALCTRIGIMVGGRLRCIGSPQHLKTRFGNHLELEVKPTEVSSADLQN 184
Query: 107 MVQRL 111
+ Q +
Sbjct: 185 LCQAI 199
>BQ273466 similar to GP|10176867|dbj contains similarity to ABC
transporter~gene_id:MAC9.5 {Arabidopsis thaliana},
partial (3%)
Length = 424
Score = 58.5 bits (140), Expect = 2e-09
Identities = 38/98 (38%), Positives = 45/98 (45%), Gaps = 50/98 (51%)
Frame = +2
Query: 35 LWNVIKLAKQDRAIILTT------------------------------------------ 52
LWNV+K AKQ+RAIILT+
Sbjct: 2 LWNVVKHAKQNRAIILTSILSLFCGF*FRSTLFCSFYLDILSLLSSHANINILSLSTILI 181
Query: 53 --------HSMEEADALCDRLGIFVDGSLQCVGYPKEL 82
HSMEEA+ALCDRLGIFV+G+LQCVG KE+
Sbjct: 182ILGFMLSAHSMEEAEALCDRLGIFVNGNLQCVGNAKEV 295
>TC234765 similar to UP|Q84M24 (Q84M24) AtABCA1, partial (9%)
Length = 535
Score = 57.4 bits (137), Expect = 4e-09
Identities = 23/38 (60%), Positives = 34/38 (88%)
Frame = +3
Query: 2 KRRLSVAISLIGDPKVVYMDEPSTGLDPASRKCLWNVI 39
KR+LSVAI++IGDP +V +DEPSTG+DP +++ +W+VI
Sbjct: 390 KRKLSVAIAMIGDPPIVILDEPSTGMDPIAKRFMWDVI 503
>AI988351 similar to GP|10176867|dbj contains similarity to ABC
transporter~gene_id:MAC9.5 {Arabidopsis thaliana},
partial (3%)
Length = 221
Score = 57.0 bits (136), Expect = 5e-09
Identities = 27/33 (81%), Positives = 31/33 (93%)
Frame = +1
Query: 148 RNFTVFAWGLSDTTLEDVFIKVAREAQAFDTLT 180
R+FTV AWGL+DTTLEDVFIKVAR AQAF+TL+
Sbjct: 1 RSFTVSAWGLADTTLEDVFIKVARGAQAFNTLS 99
>CF808489
Length = 636
Score = 54.7 bits (130), Expect = 2e-08
Identities = 24/43 (55%), Positives = 31/43 (71%)
Frame = +1
Query: 49 ILTTHSMEEADALCDRLGIFVDGSLQCVGYPKELKGRYGGTYV 91
+LTTHSMEEAD L DR+GI GSL+C+G LK R+G ++
Sbjct: 1 VLTTHSMEEADILSDRIGIMAKGSLRCIGTSIRLKSRFGTGFI 129
>TC210950 similar to UP|Q9FMU0 (Q9FMU0) Similarity to ABC transporter,
partial (51%)
Length = 803
Score = 47.8 bits (112), Expect = 3e-06
Identities = 22/63 (34%), Positives = 40/63 (62%)
Frame = +2
Query: 2 KRRLSVAISLIGDPKVVYMDEPSTGLDPASRKCLWNVIKLAKQDRAIILTTHSMEEADAL 61
KRRL++AI L+ P ++ +DEP GLD +R + ++K K++ +++ +H + E +L
Sbjct: 245 KRRLALAIQLVQTPDLLILDEPLAGLDWKARADVVKLLKHLKKELTVLVVSHDLREFASL 424
Query: 62 CDR 64
DR
Sbjct: 425 VDR 433
>CD403377
Length = 639
Score = 47.0 bits (110), Expect = 5e-06
Identities = 33/131 (25%), Positives = 63/131 (47%), Gaps = 19/131 (14%)
Frame = -1
Query: 63 DRLGIFVDGSLQCVGYPKELKGRYGGTYVFAMTTS---------------SDHEKDVENM 107
DR+GI GSL+C+G LK R+G ++ ++ + ++H + V+ +
Sbjct: 636 DRIGIMAKGSLRCIGTSIRLKSRFGAGFIANISFNGNNIECSPASGDAIFTEHHEAVKKL 457
Query: 108 VQR---LTPNANKIYHLSGTQKFELPKE-EIKMANVFRAIEAAKRNFTVFAWGLSDTTLE 163
+ + P L+ F +P + E + N F ++ ++ F + L TTLE
Sbjct: 456 FKNHLDVVPKEENNNFLT----FVIPHDREALLKNFFSELQDREKEFGISDIQLGLTTLE 289
Query: 164 DVFIKVAREAQ 174
+VF+ +AR+A+
Sbjct: 288 EVFLNIARQAE 256
>TC218418 homologue to UP|Q6X4V5 (Q6X4V5) ABC transporter, partial (49%)
Length = 1220
Score = 45.8 bits (107), Expect = 1e-05
Identities = 26/82 (31%), Positives = 46/82 (55%), Gaps = 2/82 (2%)
Frame = +1
Query: 2 KRRLSVAISLIGDPKVVYMDEPSTGLDPASRKCLWNVIK-LAKQDRAIILTTHS-MEEAD 59
KRR+S+A+ ++ P+++++DEP++GLD AS + ++ LA+ R +I + H E
Sbjct: 685 KRRVSIALEILMRPRLLFLDEPTSGLDSASAFFVTQTLRALARDGRTVIASIHQPSSEVF 864
Query: 60 ALCDRLGIFVDGSLQCVGYPKE 81
L D+L + G G E
Sbjct: 865 ELFDQLYLLSSGKTVYFGQASE 930
>TC222300 similar to GB|AAP37786.1|30725528|BT008427 At1g51560 {Arabidopsis
thaliana;} , partial (37%)
Length = 801
Score = 44.7 bits (104), Expect = 2e-05
Identities = 26/81 (32%), Positives = 48/81 (59%), Gaps = 2/81 (2%)
Frame = +1
Query: 2 KRRLSVAISLIGDPKVVYMDEPSTGLDPASRKCLWNVIK-LAKQDRAIILTTHSME-EAD 59
K+RLS+A+ ++ P+++++DEP++GLD AS + ++ +A+ +R +I + H + E
Sbjct: 97 KKRLSIAL*ILTRPRLLFLDEPTSGLDSASAFFVVQTLRNVARDERTVIYSIHQPDSEVF 276
Query: 60 ALCDRLGIFVDGSLQCVGYPK 80
AL D L + G G K
Sbjct: 277 ALYDDLFLLSGGETVYFGEAK 339
>TC215951 similar to UP|Q9SXT3 (Q9SXT3) Multidrug resistance protein
(Fragment), partial (84%)
Length = 1226
Score = 43.9 bits (102), Expect = 4e-05
Identities = 25/82 (30%), Positives = 50/82 (60%), Gaps = 2/82 (2%)
Frame = +2
Query: 3 RRLSVAISLIGDPKVVYMDEPSTGLDPASRKCLWN-VIKLAK-QDRAIILTTHSMEEADA 60
+R+++A +L P+V+ +DEP++ LDP S + + + ++KL K Q +I+ +HS+++
Sbjct: 647 QRVALARTLANSPQVLLLDEPTSALDPISTENIEDALVKLNKNQGMTVIMVSHSIKQIQR 826
Query: 61 LCDRLGIFVDGSLQCVGYPKEL 82
+ + + VDG + V P L
Sbjct: 827 IAHIVCLLVDGEIVEVLNPHNL 892
>TC207756 similar to UP|Q8RY46 (Q8RY46) Transporter associated with antigen
processing-like protein, partial (17%)
Length = 785
Score = 43.5 bits (101), Expect = 5e-05
Identities = 24/84 (28%), Positives = 48/84 (56%), Gaps = 3/84 (3%)
Frame = +3
Query: 2 KRRLSVAISLIGDPKVVYMDEPSTGLDPASRKCLWNVIKLAKQD---RAIILTTHSMEEA 58
K+R+++A +L+ DPK++ +DE ++ LD S + V++ + D R++I+ H +
Sbjct: 60 KQRIAIARALLRDPKILILDEATSALDAESEHNVKGVLRSVRSDSATRSVIVIAHRLSTI 239
Query: 59 DALCDRLGIFVDGSLQCVGYPKEL 82
A DR+ + G + +G +EL
Sbjct: 240 QA-ADRIVVMDGGEIVEMGSHREL 308
>CA820193 similar to PIR|T04229|T04 ABC-type transport protein F14M19.30 -
Arabidopsis thaliana, partial (23%)
Length = 423
Score = 42.7 bits (99), Expect = 9e-05
Identities = 24/75 (32%), Positives = 42/75 (56%), Gaps = 3/75 (4%)
Frame = +1
Query: 2 KRRLSVAISLIGDPKVVYMDEPSTGLDPASRKCLWNVIK--LAKQDRAIILTTHSMEEAD 59
+RR+S+ + L+ DP V+ +DEP++GLD S + ++K ++R IIL+ H
Sbjct: 91 RRRVSIGLCLLHDPAVLLLDEPTSGLDSTSAFKVMRILKQTCVSRNRTIILSIHQPSFKI 270
Query: 60 ALC-DRLGIFVDGSL 73
C DR+ + G +
Sbjct: 271 LACIDRILLLSKGQV 315
>TC208899 similar to UP|Q9LHD1 (Q9LHD1) Multidrug resistance p-glycoprotein,
partial (14%)
Length = 983
Score = 42.0 bits (97), Expect = 2e-04
Identities = 24/91 (26%), Positives = 47/91 (51%), Gaps = 2/91 (2%)
Frame = +3
Query: 2 KRRLSVAISLIGDPKVVYMDEPSTGLDPASRKCLWNVIKLAKQDRAIILTTHSMEEADAL 61
K+R+++A +++ +PKV+ +DE ++ LD S K + + + + R ++ H +
Sbjct: 270 KQRIAIARAILKNPKVLLLDEATSALDGPSEKVVQDTLMRVMRGRTGVVVAHRLSTIHN- 446
Query: 62 CDRLGIFVDGSLQCVGYPKEL--KGRYGGTY 90
CD +G+ G + +G L KG G Y
Sbjct: 447 CDVIGVLEKGRVVEIGTHSSLLAKGSCGAYY 539
>BI699796 similar to PIR|G85167|G85 ABC transporter like protein [imported] -
Arabidopsis thaliana, partial (13%)
Length = 421
Score = 42.0 bits (97), Expect = 2e-04
Identities = 14/39 (35%), Positives = 28/39 (70%)
Frame = +1
Query: 2 KRRLSVAISLIGDPKVVYMDEPSTGLDPASRKCLWNVIK 40
++RL++A+ L+ +P +++MDEP+TGLD + + +K
Sbjct: 268 RKRLTIAVELVANPSIIFMDEPTTGLDARAAAVVMRAVK 384
>TC208020 similar to UP|Q76CU2 (Q76CU2) PDR-type ABC transporter 1, partial
(14%)
Length = 765
Score = 41.6 bits (96), Expect = 2e-04
Identities = 15/53 (28%), Positives = 33/53 (61%), Gaps = 1/53 (1%)
Frame = +2
Query: 2 KRRLSVAISLIGDPKVVYMDEPSTGLDPASRKCLWNVIK-LAKQDRAIILTTH 53
++RL++A+ L+ +P +++MDEP++GLD + + ++ R ++ T H
Sbjct: 254 RKRLTIAVELVANPSIIFMDEPTSGLDARAAAIVMRTVRNTVDTGRTVVCTIH 412
>TC207596 similar to UP|Q7GBY1 (Q7GBY1) Tonoplast ABC transporter IDI7,
partial (35%)
Length = 1076
Score = 41.6 bits (96), Expect = 2e-04
Identities = 21/86 (24%), Positives = 46/86 (53%)
Frame = +2
Query: 2 KRRLSVAISLIGDPKVVYMDEPSTGLDPASRKCLWNVIKLAKQDRAIILTTHSMEEADAL 61
K+R+++A +L+ DPK++ +DE ++ LD S + + ++ + R +++ H +
Sbjct: 371 KQRIAIARALLMDPKILLLDEATSALDAESEYLVQDAMESLMKGRTVLVIAHRLSTVKT- 547
Query: 62 CDRLGIFVDGSLQCVGYPKELKGRYG 87
D + + DG + G +EL + G
Sbjct: 548 ADTVAVISDGQVVERGNHEELLNKNG 625
>AW317336 similar to GP|4586576|dbj| multidrug resistance protein {Cicer
arietinum}, partial (37%)
Length = 298
Score = 39.7 bits (91), Expect = 8e-04
Identities = 23/82 (28%), Positives = 49/82 (59%), Gaps = 2/82 (2%)
Frame = -1
Query: 3 RRLSVAISLIGDPKVVYMDEPSTGLDPASRKCLWN-VIKLAK-QDRAIILTTHSMEEADA 60
+R+++A +L P+V+ +DEP++ LDP S + + + ++KL+K Q +++ +HS+E
Sbjct: 262 QRVALARTLANSPQVLLLDEPTSALDPISTENIEDAMVKLSKHQGMTVLMVSHSIEHIHW 83
Query: 61 LCDRLGIFVDGSLQCVGYPKEL 82
+ + + V G + V P +
Sbjct: 82 IAHIVCMLVIGEIVQVLNPHNI 17
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.134 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,278,908
Number of Sequences: 63676
Number of extensions: 68734
Number of successful extensions: 347
Number of sequences better than 10.0: 66
Number of HSP's better than 10.0 without gapping: 344
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 345
length of query: 180
length of database: 12,639,632
effective HSP length: 91
effective length of query: 89
effective length of database: 6,845,116
effective search space: 609215324
effective search space used: 609215324
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146548.14