BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146527.10 - phase: 0 /pseudo
(129 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NP005445 cytochrome oxidase subunit I 32 0.063
TC203405 similar to GB|AAP21170.1|30102504|BT006362 At3g19170/MV... 32 0.11
TC223055 30 0.31
BQ630369 30 0.41
TC225764 similar to UP|Q8VWX2 (Q8VWX2) Gamma-tocopherol methyltr... 29 0.53
TC222915 homologue to UP|Q84P29 (Q84P29) Seed calcium dependent ... 28 0.91
BF596367 28 0.91
TC214837 similar to UP|O82346 (O82346) Expressed protein (At2g46... 27 2.0
TC214836 similar to UP|O82346 (O82346) Expressed protein (At2g46... 27 2.0
TC217213 similar to UP|Q9SE42 (Q9SE42) D-ribulose-5-phosphate 3-... 27 3.4
BF067922 similar to GP|15005009|dbj APG1 {Arabidopsis thaliana},... 26 4.5
TC214263 homologue to UP|Q8L949 (Q8L949) Plastid protein, partia... 26 5.9
TC207176 26 5.9
TC218886 homologue to GB|AAB63540.1|1871180|U90439 expressed pro... 26 5.9
TC217212 similar to UP|Q9SE42 (Q9SE42) D-ribulose-5-phosphate 3-... 26 5.9
TC229412 similar to GB|AAM19958.1|20466073|AY098948 At5g04300/At... 25 7.7
TC207236 25 7.7
BF071643 weakly similar to SP|O82528|RL15_ 60S ribosomal protein... 25 7.7
TC232258 similar to UP|Q9SBS1 (Q9SBS1) Ran GTPase activating pro... 25 7.7
TC213119 similar to UP|O49682 (O49682) N-acetylornithine deacety... 25 7.7
>NP005445 cytochrome oxidase subunit I
Length = 1584
Score = 32.3 bits (72), Expect = 0.063
Identities = 16/51 (31%), Positives = 29/51 (56%)
Frame = +1
Query: 63 FSNWNSLGPIGSYVPIIDIHDLHLSVRDVLSAPGNHTQVLYTNLPSAVENH 113
++ WN+L GSY+ ++ I + V + S+ GN+ + N+P AVE +
Sbjct: 1345 YAGWNALSSFGSYISVVGIRRFFVVV-TITSSSGNN--ITRANIPWAVEQN 1488
>TC203405 similar to GB|AAP21170.1|30102504|BT006362 At3g19170/MVI11_8
{Arabidopsis thaliana;} , partial (19%)
Length = 878
Score = 31.6 bits (70), Expect = 0.11
Identities = 19/66 (28%), Positives = 31/66 (46%)
Frame = -3
Query: 62 WFSNWNSLGPIGSYVPIIDIHDLHLSVRDVLSAPGNHTQVLYTNLPSAVENHINNIHIQF 121
WF W L +V ++ H H + V SA +H ++ LPS +NH N+H
Sbjct: 417 WFQLWLLLKCRHLFVVLLRSHQFH-HIHQVSSANWDHDKIRRRTLPSEYQNH-RNLHKLH 244
Query: 122 NDNIED 127
+ ++ D
Sbjct: 243 H*HVHD 226
>TC223055
Length = 321
Score = 30.0 bits (66), Expect = 0.31
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2
Query: 46 LKEGYTWRIGSGSSSFWFSNW 66
L+E + +R+G G SFWF W
Sbjct: 5 LREAFKFRVGRGDVSFWFDLW 67
>BQ630369
Length = 389
Score = 29.6 bits (65), Expect = 0.41
Identities = 13/32 (40%), Positives = 17/32 (52%)
Frame = +1
Query: 60 SFWFSNWNSLGPIGSYVPIIDIHDLHLSVRDV 91
SFWFSNW PI + I HL++ +V
Sbjct: 223 SFWFSNWLGSWPICLDENFVHIEHAHLTISEV 318
>TC225764 similar to UP|Q8VWX2 (Q8VWX2) Gamma-tocopherol methyltransferase,
partial (38%)
Length = 551
Score = 29.3 bits (64), Expect = 0.53
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +2
Query: 52 WRIGSGSSSFWFSNWNSLGPIGSY 75
WRI SGSS+ W N +++ P GS+
Sbjct: 23 WRISSGSSTRWHHNNSNMVPQGSW 94
>TC222915 homologue to UP|Q84P29 (Q84P29) Seed calcium dependent protein
kinase a, partial (45%)
Length = 688
Score = 28.5 bits (62), Expect = 0.91
Identities = 14/32 (43%), Positives = 19/32 (58%), Gaps = 3/32 (9%)
Frame = -2
Query: 36 WSSIIRAKNILKEGYTWRIGSGS---SSFWFS 64
W +I+RA N + +G+TW I G SSF S
Sbjct: 174 WCNIVRASNNVTKGFTWLIKHGEPKISSFELS 79
>BF596367
Length = 419
Score = 28.5 bits (62), Expect = 0.91
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +3
Query: 35 TWSSIIRAKNILKEGYTWRIGSGSS 59
+WSS+ +K++ + G WRIG G S
Sbjct: 213 SWSSV**SKDLAEHGAIWRIGDGRS 287
>TC214837 similar to UP|O82346 (O82346) Expressed protein
(At2g46230/T3F17.12), partial (80%)
Length = 1080
Score = 27.3 bits (59), Expect = 2.0
Identities = 16/61 (26%), Positives = 31/61 (50%)
Frame = +2
Query: 43 KNILKEGYTWRIGSGSSSFWFSNWNSLGPIGSYVPIIDIHDLHLSVRDVLSAPGNHTQVL 102
KN++KE + + SS+ +F +LGP Y ++D + ++ S+++ L L
Sbjct: 215 KNLMKEKLPRNVPTHSSALFFQYNTALGP--PYRVLVDTNFINFSIQNKLDLEKGMMDCL 388
Query: 103 Y 103
Y
Sbjct: 389 Y 391
>TC214836 similar to UP|O82346 (O82346) Expressed protein
(At2g46230/T3F17.12), partial (64%)
Length = 526
Score = 27.3 bits (59), Expect = 2.0
Identities = 16/61 (26%), Positives = 31/61 (50%)
Frame = +1
Query: 43 KNILKEGYTWRIGSGSSSFWFSNWNSLGPIGSYVPIIDIHDLHLSVRDVLSAPGNHTQVL 102
KN++KE + + SS+ +F +LGP Y ++D + ++ S+++ L L
Sbjct: 238 KNLMKEKLPRNVPTHSSALFFQYNTALGP--PYRVLVDTNFINFSIQNKLDLEKGMMDCL 411
Query: 103 Y 103
Y
Sbjct: 412 Y 414
>TC217213 similar to UP|Q9SE42 (Q9SE42) D-ribulose-5-phosphate 3-epimerase ,
partial (74%)
Length = 646
Score = 26.6 bits (57), Expect = 3.4
Identities = 15/43 (34%), Positives = 21/43 (47%)
Frame = -2
Query: 43 KNILKEGYTWRIGSGSSSFWFSNWNSLGPIGSYVPIIDIHDLH 85
K I T +I S F+NW++ IG +PI D+H H
Sbjct: 213 KRICNHKVTIQISLCVLSQTFNNWSANS*IGDKMPIHDVHVEH 85
>BF067922 similar to GP|15005009|dbj APG1 {Arabidopsis thaliana}, partial
(21%)
Length = 422
Score = 26.2 bits (56), Expect = 4.5
Identities = 9/27 (33%), Positives = 19/27 (70%)
Frame = -3
Query: 4 STNKLWVSLLSNKYTGGHNTLLASASS 30
+TN++W+ + S ++ GGH + +AS+
Sbjct: 111 ATNEVWILMSSIEH*GGHGLIYGNASN 31
>TC214263 homologue to UP|Q8L949 (Q8L949) Plastid protein, partial (47%)
Length = 581
Score = 25.8 bits (55), Expect = 5.9
Identities = 15/64 (23%), Positives = 29/64 (44%)
Frame = -1
Query: 54 IGSGSSSFWFSNWNSLGPIGSYVPIIDIHDLHLSVRDVLSAPGNHTQVLYTNLPSAVENH 113
+ +G + F+ W L + VP SV V+S H+Q++ + P +++ H
Sbjct: 371 LDAGHTLFYHCIWVCLDAFVAVVP-------PFSVFQVISERSLHSQIIQRHSPCSLDQH 213
Query: 114 INNI 117
N +
Sbjct: 212 TNPV 201
>TC207176
Length = 1616
Score = 25.8 bits (55), Expect = 5.9
Identities = 19/60 (31%), Positives = 28/60 (46%)
Frame = +3
Query: 10 VSLLSNKYTGGHNTLLASASSNSSPTWSSIIRAKNILKEGYTWRIGSGSSSFWFSNWNSL 69
+ LL ++ TG T +S SS+ S KN GY+ R+G S + W+SL
Sbjct: 993 LELLGDETTGTGLTSWSSFSSSCSSD-EEFCSGKNWKLGGYSARVGRRSEAIVCYRWSSL 1169
>TC218886 homologue to GB|AAB63540.1|1871180|U90439 expressed protein
{Arabidopsis thaliana;} , partial (26%)
Length = 895
Score = 25.8 bits (55), Expect = 5.9
Identities = 11/21 (52%), Positives = 17/21 (80%)
Frame = -1
Query: 26 ASASSNSSPTWSSIIRAKNIL 46
+S+SSNSSP SS++R ++ L
Sbjct: 178 SSSSSNSSPMLSSLLRVEHFL 116
>TC217212 similar to UP|Q9SE42 (Q9SE42) D-ribulose-5-phosphate 3-epimerase ,
partial (94%)
Length = 843
Score = 25.8 bits (55), Expect = 5.9
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = -2
Query: 43 KNILKEGYTWRIGSGSSSFWFSNWNSLGPIGSYVPIIDIH 82
K I T +I S F+NW++ IG +PI D+H
Sbjct: 263 KRICNHKVTIQISLCVLSQTFNNWSANS*IGDKMPIHDVH 144
>TC229412 similar to GB|AAM19958.1|20466073|AY098948 At5g04300/At5g04300
{Arabidopsis thaliana;} , partial (37%)
Length = 925
Score = 25.4 bits (54), Expect = 7.7
Identities = 16/55 (29%), Positives = 24/55 (43%)
Frame = -3
Query: 55 GSGSSSFWFSNWNSLGPIGSYVPIIDIHDLHLSVRDVLSAPGNHTQVLYTNLPSA 109
G W ++W L + S P+ LHL + + S+ NH+ LY P A
Sbjct: 362 GQFEQRIWLAHWWPLRTVHSAPPLRVQRTLHLPLCPLPSSNSNHS-TLYRLCPRA 201
>TC207236
Length = 677
Score = 25.4 bits (54), Expect = 7.7
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +1
Query: 52 WRIGSGSSSFWFSNWN 67
W+ G G FW S+WN
Sbjct: 496 WKTGYGIHGFWPSSWN 543
>BF071643 weakly similar to SP|O82528|RL15_ 60S ribosomal protein L15.
[Petunia] {Petunia hybrida}, partial (32%)
Length = 331
Score = 25.4 bits (54), Expect = 7.7
Identities = 13/22 (59%), Positives = 14/22 (63%)
Frame = -1
Query: 74 SYVPIIDIHDLHLSVRDVLSAP 95
S V II HDL + VRDVL P
Sbjct: 253 STVGIIQHHDLKIFVRDVLIVP 188
>TC232258 similar to UP|Q9SBS1 (Q9SBS1) Ran GTPase activating protein,
partial (48%)
Length = 985
Score = 25.4 bits (54), Expect = 7.7
Identities = 12/28 (42%), Positives = 16/28 (56%)
Frame = +2
Query: 10 VSLLSNKYTGGHNTLLASASSNSSPTWS 37
V L+S + GGH L+ S +S TWS
Sbjct: 464 VVLISKAF*GGHGQLIEVDLSTNSITWS 547
>TC213119 similar to UP|O49682 (O49682) N-acetylornithine deacetylase-like
protein, (Fragment), partial (27%)
Length = 479
Score = 25.4 bits (54), Expect = 7.7
Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 2/36 (5%)
Frame = -1
Query: 76 VPIIDI--HDLHLSVRDVLSAPGNHTQVLYTNLPSA 109
+PI+ I HD+H++ + GN VL+ +PSA
Sbjct: 368 IPIVGIGGHDVHVASDEREDFAGNGAGVLHDEVPSA 261
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.314 0.130 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,367,027
Number of Sequences: 63676
Number of extensions: 113656
Number of successful extensions: 544
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 544
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 544
length of query: 129
length of database: 12,639,632
effective HSP length: 86
effective length of query: 43
effective length of database: 7,163,496
effective search space: 308030328
effective search space used: 308030328
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 53 (25.0 bits)
Medicago: description of AC146527.10