BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146343.8 + phase: 0 /pseudo
(379 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC216575 similar to UP|Q6K739 (Q6K739) Bile acid:sodium symporte... 464 e-131
CD400764 192 4e-59
BI968138 146 2e-43
CO984735 108 4e-24
TC219322 weakly similar to GB|CAA16569.1|2827561|ATT12H17 predic... 85 6e-17
TC219321 similar to GB|CAA16569.1|2827561|ATT12H17 predicted pro... 47 1e-05
TC230657 35 0.056
TC233819 weakly similar to GB|CAA16569.1|2827561|ATT12H17 predic... 35 0.073
TC216218 homologue to UP|Q9M7P3 (Q9M7P3) GTP-binding protein, co... 32 0.62
TC228915 31 0.81
TC207588 weakly similar to UP|Q9SHL8 (Q9SHL8) Expressed protein,... 30 2.3
TC214303 similar to UP|Q9T0K5 (Q9T0K5) Extensin-like protein, pa... 29 3.1
BQ610089 homologue to SP|Q9FKC0|R1AD_ 60S ribosomal protein L13a... 29 3.1
TC218038 similar to UP|Q9FJM0 (Q9FJM0) GTP binding protein-like,... 29 4.0
TC214085 similar to UP|Q93X25 (Q93X25) 2-Cys peroxiredoxin, part... 28 5.2
TC210019 similar to UP|Q84KK5 (Q84KK5) S-adenosyl-L-methionine: ... 28 5.2
TC234924 28 5.2
CD392268 similar to GP|21324025|dbj Predicted Na+-dependent tran... 28 6.8
TC219071 similar to GB|AAP21342.1|30102848|BT006534 At3g49420 {A... 28 6.8
TC214163 similar to UP|Q9FE12 (Q9FE12) Peroxiredoxin precursor, ... 28 6.8
>TC216575 similar to UP|Q6K739 (Q6K739) Bile acid:sodium symporter-like,
partial (73%)
Length = 1829
Score = 464 bits (1194), Expect = e-131
Identities = 259/344 (75%), Positives = 284/344 (82%), Gaps = 7/344 (2%)
Frame = +3
Query: 1 MASIPVFSPFTPMKQKHKPLSFQPLLRTRSSFSLQRVSK------GCSISSFACSTSKLH 54
MASIPV P Q PL FQ R+RS+ S RV + G + SSFACSTS+
Sbjct: 228 MASIPVACSTHPRLQA-SPL-FQSHARSRSA-SFWRVQQRGSMGGGGAASSFACSTSQFR 398
Query: 55 GG-RVGFNHREGKTSFLKFGVDESVVGGGGNGDVGERDWSQILSALLPFVVAVTAVAALS 113
GG F+ R+GK S L FG D+ G G+ ERD SQ+LSALLP VVA TAVAAL+
Sbjct: 399 GGFGFRFHRRQGKFSLLSFGGDDD--GSAVGGEARERDLSQLLSALLPVVVAATAVAALA 572
Query: 114 QPSTFTWVSKELYAPALGGIMLSIGIGLSMEDFALAFKRPLPLSIGFIAQYVLKPVLGVL 173
+PSTFTWVSKELYAPALGGIMLSIGI LS++DFALAFKRPLPL+IG IAQYVLKPVLG+L
Sbjct: 573 KPSTFTWVSKELYAPALGGIMLSIGIRLSVDDFALAFKRPLPLTIGLIAQYVLKPVLGIL 752
Query: 174 IAKAFGLPRMFYAGFVLTACVSGAQLSSYASFISKGDVALCIVLTSYTTIASVIVTPLLT 233
+AKAFGL RMFYAGFVLTACVSGAQLSSYA+F+SKGDVAL I+LTSYTTIASVIVTPLLT
Sbjct: 753 VAKAFGLSRMFYAGFVLTACVSGAQLSSYANFLSKGDVALGILLTSYTTIASVIVTPLLT 932
Query: 234 GLLIGSVVPVDAVAMSKSILQVVLAPVTLGLLLNTYAKPVVSILRPVMPFVAMICTSLCI 293
GLLIGSVVPVDAVAMSKSILQVVL PVTLGLLLNTYAK VVS+L+PVMPFVAMICTSLCI
Sbjct: 933 GLLIGSVVPVDAVAMSKSILQVVLVPVTLGLLLNTYAKSVVSVLQPVMPFVAMICTSLCI 1112
Query: 294 GSPLAINRSQILSGEGLRLVAPVLIFHAAAFTLGYWFSNLPSLR 337
GSPLA+NRSQIL+GEGLRLV PVL FH AFTLGYWFS +PSLR
Sbjct: 1113GSPLALNRSQILTGEGLRLVFPVLTFHVVAFTLGYWFSKIPSLR 1244
Score = 56.6 bits (135), Expect = 2e-08
Identities = 24/44 (54%), Positives = 30/44 (67%)
Frame = +2
Query: 336 LRFLTSITINTLSQAGRTGEQDNFIMHWNAELHTCRPSSNTIFG 379
L F + +++ +AGRTGEQD+F MHWNAE H CR N IFG
Sbjct: 1202 LHFRILVLKDSILEAGRTGEQDDFTMHWNAEFHPCRTPCNAIFG 1333
>CD400764
Length = 551
Score = 192 bits (488), Expect(2) = 4e-59
Identities = 100/110 (90%), Positives = 107/110 (96%)
Frame = -2
Query: 200 SSYASFISKGDVALCIVLTSYTTIASVIVTPLLTGLLIGSVVPVDAVAMSKSILQVVLAP 259
SSYA+F+SKGDVAL I+LTSYTTIASVIVTPLLTGLLIGSVVPVDAVAMSKSILQVVL P
Sbjct: 550 SSYANFLSKGDVALGILLTSYTTIASVIVTPLLTGLLIGSVVPVDAVAMSKSILQVVLVP 371
Query: 260 VTLGLLLNTYAKPVVSILRPVMPFVAMICTSLCIGSPLAINRSQILSGEG 309
VTLGLLLNTYAK VVS+L+PVMPFVAMICTSLCIGSPLA+NRSQIL+GEG
Sbjct: 370 VTLGLLLNTYAKSVVSVLQPVMPFVAMICTSLCIGSPLALNRSQILTGEG 221
Score = 54.3 bits (129), Expect(2) = 4e-59
Identities = 22/32 (68%), Positives = 25/32 (77%)
Frame = -3
Query: 348 SQAGRTGEQDNFIMHWNAELHTCRPSSNTIFG 379
++AGRTGEQD+F MHWNAE H CR N IFG
Sbjct: 228 AKAGRTGEQDDFTMHWNAEFHPCRTPCNAIFG 133
>BI968138
Length = 554
Score = 146 bits (369), Expect(2) = 2e-43
Identities = 74/102 (72%), Positives = 86/102 (83%)
Frame = -2
Query: 219 SYTTIASVIVTPLLTGLLIGSVVPVDAVAMSKSILQVVLAPVTLGLLLNTYAKPVVSILR 278
SY +A+VIVTPL G+ I SVV VDAVAMSKSIL+VVL PV LG+L+NTYAK S+L+
Sbjct: 547 SYXAVAAVIVTPLXXGVXIXSVVXVDAVAMSKSILRVVLVPVALGILVNTYAKSXXSVLQ 368
Query: 279 PVMPFVAMICTSLCIGSPLAINRSQILSGEGLRLVAPVLIFH 320
PVMPFVAMICTSLCIGSP A++RSQ+ +GEGLRLV PVL FH
Sbjct: 367 PVMPFVAMICTSLCIGSPRALSRSQVSTGEGLRLVFPVLTFH 242
Score = 47.8 bits (112), Expect(2) = 2e-43
Identities = 22/45 (48%), Positives = 27/45 (59%)
Frame = -3
Query: 335 SLRFLTSITINTLSQAGRTGEQDNFIMHWNAELHTCRPSSNTIFG 379
SL F + +++ +AGRT EQ F MHWNAE H CR N I G
Sbjct: 237 SLHFRIVVLKDSILEAGRTEEQVAFTMHWNAEFHPCRTPCNAIVG 103
Score = 27.7 bits (60), Expect = 8.9
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = -1
Query: 324 FTLGYWFSNLPSLR 337
FTLG WFS +PSLR
Sbjct: 233 FTLG*WFSKIPSLR 192
>CO984735
Length = 730
Score = 108 bits (270), Expect = 4e-24
Identities = 62/166 (37%), Positives = 100/166 (59%)
Frame = -1
Query: 186 AGFVLTACVSGAQLSSYASFISKGDVALCIVLTSYTTIASVIVTPLLTGLLIGSVVPVDA 245
AG +L C G S+ +++++ +VAL +++T+ +TI+++I+TPLLT L G V VDA
Sbjct: 727 AGIILLGCCPGGTASNIITYLARANVALSVIMTTASTISAMIMTPLLTAKLAGKYVAVDA 548
Query: 246 VAMSKSILQVVLAPVTLGLLLNTYAKPVVSILRPVMPFVAMICTSLCIGSPLAINRSQIL 305
+ S LQVVL PV G LN + +P+V + P+MP +A+I ++ G+ + + S IL
Sbjct: 547 PGLLISTLQVVLVPVLAGAFLNQFFQPIVKFVSPLMPPIAVITVAILCGNAIGQSSSAIL 368
Query: 306 SGEGLRLVAPVLIFHAAAFTLGYWFSNLPSLRFLTSITINTLSQAG 351
+ G ++A L+ A+ F GY F+ L L +S TI+ SQ G
Sbjct: 367 ASGGQVILATFLL-QASGFFFGYLFARLLRLDVSSSRTIS--SQVG 239
>TC219322 weakly similar to GB|CAA16569.1|2827561|ATT12H17 predicted protein
{Arabidopsis thaliana;} , partial (37%)
Length = 768
Score = 84.7 bits (208), Expect = 6e-17
Identities = 47/135 (34%), Positives = 77/135 (56%), Gaps = 2/135 (1%)
Frame = +3
Query: 228 VTPLLTGLLIGSVVPVDAVAMSKSILQVVLAPVTLGLLLNTYAKPVVSILRPVMPFVAMI 287
VTPLL LLIG +P+D M SI Q+V+ P+ GLLLN + + +++RP +P ++++
Sbjct: 12 VTPLLLLLLIGKKLPIDVRGMVYSITQIVVVPIAAGLLLNRFYPRICNVIRPFLPPLSVL 191
Query: 288 CTSLCIGSPLAINRSQILSGEGLRLVAPVLIFHAAAFTLGYWFSNLPSLRFLTSITINTL 347
S+C G+PLA N + S G+ ++ V+ FH ++F GY S F S+ + L
Sbjct: 192 VASICAGAPLAFNVETMKSPLGVAILLLVVAFHLSSFIAGYILSGFV---FRDSLDVKAL 362
Query: 348 SQ--AGRTGEQDNFI 360
+ + TG Q + +
Sbjct: 363 QRTISFETGMQSSLL 407
>TC219321 similar to GB|CAA16569.1|2827561|ATT12H17 predicted protein
{Arabidopsis thaliana;} , partial (26%)
Length = 693
Score = 47.0 bits (110), Expect = 1e-05
Identities = 25/90 (27%), Positives = 50/90 (54%), Gaps = 2/90 (2%)
Frame = +3
Query: 273 VVSILRPVMPFVAMICTSLCIGSPLAINRSQILSGEGLRLVAPVLIFHAAAFTLGYWFSN 332
+ +++RP +P ++++ S+C G+PLA+N + S G+ ++ V+ FH ++F GY S
Sbjct: 9 ICNVIRPFLPPLSVLVASICAGAPLALNVETMKSPLGVAILLLVVAFHLSSFIAGYILSG 188
Query: 333 LPSLRFLTSITINTLSQ--AGRTGEQDNFI 360
F S+ + L + + TG Q + +
Sbjct: 189 FV---FRDSLDVKALQRTISFETGMQSSLL 269
>TC230657
Length = 700
Score = 35.0 bits (79), Expect = 0.056
Identities = 25/122 (20%), Positives = 58/122 (47%), Gaps = 12/122 (9%)
Frame = +2
Query: 160 FIAQYVLKPVLGVLIAKAFGLPRMFYA----------GFVLTACVSGA--QLSSYASFIS 207
F +Y++ PV G+++ A + + V TA GA + ++Y
Sbjct: 29 FTPRYII-PVAGMMVGNAMTVTGVTMKRLRDDIKAQMNLVETALALGASPRQATYEQVKR 205
Query: 208 KGDVALCIVLTSYTTIASVIVTPLLTGLLIGSVVPVDAVAMSKSILQVVLAPVTLGLLLN 267
+AL V+ + T+ + + +TGL++G P++A+ + ++ +++ T+ +++
Sbjct: 206 ALVIALSPVVDNAKTVGLISLPGAMTGLIMGGASPLEAIQLQIVVMNMLIGASTISSIMS 385
Query: 268 TY 269
TY
Sbjct: 386 TY 391
>TC233819 weakly similar to GB|CAA16569.1|2827561|ATT12H17 predicted protein
{Arabidopsis thaliana;} , partial (8%)
Length = 449
Score = 34.7 bits (78), Expect = 0.073
Identities = 15/38 (39%), Positives = 23/38 (60%)
Frame = +2
Query: 97 SALLPFVVAVTAVAALSQPSTFTWVSKELYAPALGGIM 134
++ LP V+ + + AL P + TW + YAPALG +M
Sbjct: 335 NSYLPHVLIASILLALIYPPSLTWFTSRYYAPALGFLM 448
>TC216218 homologue to UP|Q9M7P3 (Q9M7P3) GTP-binding protein, complete
Length = 1545
Score = 31.6 bits (70), Expect = 0.62
Identities = 19/55 (34%), Positives = 27/55 (48%)
Frame = -3
Query: 296 PLAINRSQILSGEGLRLVAPVLIFHAAAFTLGYWFSNLPSLRFLTSITINTLSQA 350
P +N I+S V P ++ AAFT S+ S F+TS IN LS++
Sbjct: 1240 PETLNLKNIMSPSWTTYVFPSCLYFPAAFTADSLPSSFRSSNFITSAQINPLSKS 1076
>TC228915
Length = 1239
Score = 31.2 bits (69), Expect = 0.81
Identities = 26/65 (40%), Positives = 34/65 (52%), Gaps = 3/65 (4%)
Frame = +2
Query: 145 DFALAFKRPLPLSIGFIAQYVLKPVLGVLIAK---AFGLPRMFYAGFVLTACVSGAQLSS 201
D+AL F PLPL IG +A Y L LGV +A FG+ + +L A S A S+
Sbjct: 323 DYALVFGIPLPL-IG-MAAYGLVAALGVQLASKNFRFGIEKPTAEAVLLGATTSMAAASA 496
Query: 202 YASFI 206
Y +I
Sbjct: 497 YFLYI 511
>TC207588 weakly similar to UP|Q9SHL8 (Q9SHL8) Expressed protein, partial
(35%)
Length = 1119
Score = 29.6 bits (65), Expect = 2.3
Identities = 26/92 (28%), Positives = 41/92 (44%), Gaps = 7/92 (7%)
Frame = +2
Query: 152 RPLPLSIGFIAQYVLKPVLGVLIAKA---FGL----PRMFYAGFVLTACVSGAQLSSYAS 204
+PL L+ IA+ L PV+G+ I +A GL P Y + A +S+ A
Sbjct: 308 KPLTLTSIIIARLFLLPVIGLFIVRAAANLGLLPVDPLFQYVLVMQYAMPPAMNISTVAQ 487
Query: 205 FISKGDVALCIVLTSYTTIASVIVTPLLTGLL 236
G+ ++L T A++ +T T LL
Sbjct: 488 LFEVGNEECSVILLWTYTAAAIALTAWSTFLL 583
>TC214303 similar to UP|Q9T0K5 (Q9T0K5) Extensin-like protein, partial (18%)
Length = 1014
Score = 29.3 bits (64), Expect = 3.1
Identities = 16/53 (30%), Positives = 29/53 (54%)
Frame = -2
Query: 210 DVALCIVLTSYTTIASVIVTPLLTGLLIGSVVPVDAVAMSKSILQVVLAPVTL 262
+VA+ +V+ +Y + V+V ++ + SVV V V +++VV VTL
Sbjct: 371 EVAVMVVVVNYIELGEVVVMVMVADGKVQSVVMVVVVNALHKVVEVVAEEVTL 213
>BQ610089 homologue to SP|Q9FKC0|R1AD_ 60S ribosomal protein L13a-4.
[Mouse-ear cress] {Arabidopsis thaliana}, partial (44%)
Length = 415
Score = 29.3 bits (64), Expect = 3.1
Identities = 22/81 (27%), Positives = 33/81 (40%)
Frame = +2
Query: 47 ACSTSKLHGGRVGFNHREGKTSFLKFGVDESVVGGGGNGDVGERDWSQILSALLPFVVAV 106
+C + H G G + EG + + + G GG GD+ R Q +L F
Sbjct: 110 SCGRAAPHAGEAGIDRGEGTSEWAEGG------GGALRGDLHLRWARQAEDEVLAFPPQA 271
Query: 107 TAVAALSQPSTFTWVSKELYA 127
ALS+P F ++L A
Sbjct: 272 HEHQALSRPHPFPCTRQDLLA 334
>TC218038 similar to UP|Q9FJM0 (Q9FJM0) GTP binding protein-like, partial
(62%)
Length = 1529
Score = 28.9 bits (63), Expect = 4.0
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = -2
Query: 271 KPVVSILRPVMPFVAMICTSLCIGSPLAINRS 302
KP VS+ R PF ICT L +GS +A N S
Sbjct: 550 KPTVSVRRNSFPF--FICTLLVVGSKVANNLS 461
>TC214085 similar to UP|Q93X25 (Q93X25) 2-Cys peroxiredoxin, partial (84%)
Length = 1129
Score = 28.5 bits (62), Expect = 5.2
Identities = 16/36 (44%), Positives = 17/36 (46%)
Frame = +3
Query: 5 PVFSPFTPMKQKHKPLSFQPLLRTRSSFSLQRVSKG 40
P P P H P S PLLR +S LQR S G
Sbjct: 270 PFSLPHAPFSLPHAPFSLSPLLRCQS--LLQRASAG 371
>TC210019 similar to UP|Q84KK5 (Q84KK5) S-adenosyl-L-methionine: daidzein
7-0-methyltransferase, complete
Length = 1156
Score = 28.5 bits (62), Expect = 5.2
Identities = 30/100 (30%), Positives = 47/100 (47%), Gaps = 4/100 (4%)
Frame = -3
Query: 184 FYAGFVLTACVSGAQLSSYASFISKGDVALCIVLTSYTTIASVIVTPLLTGLLIGSVVPV 243
F++ L+ ++ A + S SF S V LC S+ T + +I PLL I S+
Sbjct: 992 FHSSSFLSFPLTHAMVISITSFSS---VTLCSSSFSFITTSMIITFPLLPLFEIASLQFF 822
Query: 244 DAVAMSKSI--LQVVLAPVT--LGLLLNTYAKPVVSILRP 279
+ S SI ++ L+ +T LG+ LN V +L P
Sbjct: 821 NIFMQSLSIQLCRIHLSRITSALGIDLNMSPPTYVKLLLP 702
>TC234924
Length = 506
Score = 28.5 bits (62), Expect = 5.2
Identities = 12/29 (41%), Positives = 18/29 (61%)
Frame = -2
Query: 270 AKPVVSILRPVMPFVAMICTSLCIGSPLA 298
A PV +L+P+ PF+ ++ T IG P A
Sbjct: 490 AAPVTVLLQPLDPFLILLTTESVIGGPAA 404
>CD392268 similar to GP|21324025|dbj Predicted Na+-dependent transporter
{Corynebacterium glutamicum ATCC 13032}, partial (6%)
Length = 393
Score = 28.1 bits (61), Expect = 6.8
Identities = 8/26 (30%), Positives = 22/26 (83%)
Frame = -2
Query: 202 YASFISKGDVALCIVLTSYTTIASVI 227
+ +++S+GDVAL +++T+ +T+ +++
Sbjct: 392 HEAYLSRGDVALSVIMTTASTLTAMV 315
>TC219071 similar to GB|AAP21342.1|30102848|BT006534 At3g49420 {Arabidopsis
thaliana;} , complete
Length = 669
Score = 28.1 bits (61), Expect = 6.8
Identities = 21/67 (31%), Positives = 36/67 (53%), Gaps = 2/67 (2%)
Frame = +3
Query: 130 LGGIMLSIGIGLSMEDFALA--FKRPLPLSIGFIAQYVLKPVLGVLIAKAFGLPRMFYAG 187
+ G+ L+IG+ +M+ F FK + IGF + P+LG +I +++G +F +G
Sbjct: 306 VSGVSLTIGLKSTMQFFMKRSNFKGTISFGIGFFILILGWPILG-MIVESYGFIILF-SG 479
Query: 188 FVLTACV 194
F T V
Sbjct: 480 FWPTLAV 500
>TC214163 similar to UP|Q9FE12 (Q9FE12) Peroxiredoxin precursor, partial
(48%)
Length = 548
Score = 28.1 bits (61), Expect = 6.8
Identities = 17/36 (47%), Positives = 19/36 (52%)
Frame = +1
Query: 3 SIPVFSPFTPMKQKHKPLSFQPLLRTRSSFSLQRVS 38
S P SP P H PL+ PLLR +S LQR S
Sbjct: 148 SPPQPSPHAPFSLPHAPLAHSPLLRRQS--LLQRAS 249
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.324 0.138 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,501,627
Number of Sequences: 63676
Number of extensions: 233225
Number of successful extensions: 1979
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 1936
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1977
length of query: 379
length of database: 12,639,632
effective HSP length: 99
effective length of query: 280
effective length of database: 6,335,708
effective search space: 1773998240
effective search space used: 1773998240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146343.8