BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146343.12 + phase: 0 /pseudo
(176 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC205856 homologue to UP|Q6SPR3 (Q6SPR3) Ribosomal protein S6, c... 342 5e-95
TC203633 homologue to UP|Q6SPR3 (Q6SPR3) Ribosomal protein S6, c... 338 6e-94
TC203798 homologue to UP|Q6SPR3 (Q6SPR3) Ribosomal protein S6, c... 337 1e-93
AW472434 298 1e-81
AW704982 266 3e-72
BG352914 homologue to SP|Q9M3V8|RS6_A 40S ribosomal protein S6. ... 162 1e-40
BG239336 159 5e-40
BE807460 similar to GP|9931636|gb| ribosomal protein s6 RPS6-2 {... 116 5e-27
TC203351 homologue to UP|Q6SPR3 (Q6SPR3) Ribosomal protein S6, p... 80 6e-16
AI856824 similar to SP|Q9M3V8|RS6_A 40S ribosomal protein S6. [G... 69 1e-12
BI785648 homologue to GP|22296425|dbj 40S ribosomal protein S6 {... 48 2e-06
CA938560 homologue to GP|22296425|dbj 40S ribosomal protein S6 {... 48 3e-06
BF070100 30 0.45
TC226462 homologue to UP|PCNA_PEA (O82134) Proliferating cell nu... 30 0.77
TC203554 homologue to UP|RL38_ARATH (O22860) 60S ribosomal prote... 29 1.3
TC235033 29 1.3
AW597075 similar to PIR|T47436|T474 protein kinase-like protein ... 28 2.2
TC223944 similar to GB|AAP68218.1|31711724|BT008779 At5g51910 {A... 28 2.9
TC226950 weakly similar to UP|Q7XCK3 (Q7XCK3) Mucin-like protein... 27 5.0
TC208776 similar to UP|SGPL_ARATH (Q9C509) Sphingosine-1-phospha... 27 5.0
>TC205856 homologue to UP|Q6SPR3 (Q6SPR3) Ribosomal protein S6, complete
Length = 1037
Score = 342 bits (877), Expect = 5e-95
Identities = 164/174 (94%), Positives = 170/174 (97%)
Frame = +2
Query: 1 FNVANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFP 60
FN+ANPTTGCQKKLEIDDDQKLRAFWDKRISQEV+GDALGEEFKGYVFKITGGCDKQGFP
Sbjct: 86 FNIANPTTGCQKKLEIDDDQKLRAFWDKRISQEVSGDALGEEFKGYVFKITGGCDKQGFP 265
Query: 61 MKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEND 120
MKQGVLTPGRVRLLLHRGTPCFRG+GRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEND
Sbjct: 266 MKQGVLTPGRVRLLLHRGTPCFRGYGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEND 445
Query: 121 LPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDGAKLPR 174
LPGLTD EKPRMRGPKRASKIRKLFNLSK+DDVRKYVNTYRRTFTTK G K+ +
Sbjct: 446 LPGLTDAEKPRMRGPKRASKIRKLFNLSKEDDVRKYVNTYRRTFTTKAGKKVSK 607
>TC203633 homologue to UP|Q6SPR3 (Q6SPR3) Ribosomal protein S6, complete
Length = 1053
Score = 338 bits (868), Expect = 6e-94
Identities = 163/174 (93%), Positives = 170/174 (97%)
Frame = +2
Query: 1 FNVANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFP 60
FN+ANPTTGCQKKLEIDDD KLRAFWDKRISQEV GDALGEEFKGYVFKITGGCDKQGFP
Sbjct: 65 FNIANPTTGCQKKLEIDDDLKLRAFWDKRISQEVVGDALGEEFKGYVFKITGGCDKQGFP 244
Query: 61 MKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEND 120
MKQGVLTPGRVRLLLHRGTPCFRG+GRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGE+D
Sbjct: 245 MKQGVLTPGRVRLLLHRGTPCFRGYGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGESD 424
Query: 121 LPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDGAKLPR 174
LPGLTDVEKPRMRGPKRASKIRKLFNLSK+DDVRKYVNTYRRTFTTK+G K+ +
Sbjct: 425 LPGLTDVEKPRMRGPKRASKIRKLFNLSKEDDVRKYVNTYRRTFTTKNGKKVSK 586
>TC203798 homologue to UP|Q6SPR3 (Q6SPR3) Ribosomal protein S6, complete
Length = 1012
Score = 337 bits (865), Expect = 1e-93
Identities = 163/174 (93%), Positives = 169/174 (96%)
Frame = +1
Query: 1 FNVANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFP 60
FN+ANPTTGCQKKLEIDDD KLRAFWDKRISQEV GDALGEEFKGYVFKITGGCDKQGFP
Sbjct: 64 FNIANPTTGCQKKLEIDDDLKLRAFWDKRISQEVLGDALGEEFKGYVFKITGGCDKQGFP 243
Query: 61 MKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEND 120
MKQGVLTPGRVRLLLHRGTPCFRG+GRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGE+D
Sbjct: 244 MKQGVLTPGRVRLLLHRGTPCFRGYGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGESD 423
Query: 121 LPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDGAKLPR 174
LPGLTDVEKPRMRGPKRASKIRKLFNLSK+DDVRKYVNTYRRTFTTK G K+ +
Sbjct: 424 LPGLTDVEKPRMRGPKRASKIRKLFNLSKEDDVRKYVNTYRRTFTTKAGKKVSK 585
>AW472434
Length = 475
Score = 298 bits (762), Expect = 1e-81
Identities = 141/157 (89%), Positives = 153/157 (96%)
Frame = +2
Query: 1 FNVANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFP 60
FN+A+PTTGCQKKLEIDDDQKLRAFWDKRISQEV+GDALGEEFKGYVF+ITGGCDKQGFP
Sbjct: 5 FNMASPTTGCQKKLEIDDDQKLRAFWDKRISQEVSGDALGEEFKGYVFRITGGCDKQGFP 184
Query: 61 MKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEND 120
MKQGVLTPGRVRLLLHRGTPCFRG+GRR+GERRRKSVRGCI SPDLSVLNLVIVKKGEND
Sbjct: 185 MKQGVLTPGRVRLLLHRGTPCFRGYGRRDGERRRKSVRGCIGSPDLSVLNLVIVKKGEND 364
Query: 121 LPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYV 157
+PGLTD EKPRMRGPKRA K+R+LFNL ++DDVRK+V
Sbjct: 365 VPGLTDAEKPRMRGPKRACKMRELFNLCEEDDVRKWV 475
>AW704982
Length = 538
Score = 266 bits (681), Expect = 3e-72
Identities = 132/165 (80%), Positives = 142/165 (86%)
Frame = +2
Query: 1 FNVANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFP 60
FN+ANPTTGCQKKLEIDDD KLRAFWDKRISQEV GDAL EEFKGYVF+ITG CD Q FP
Sbjct: 5 FNIANPTTGCQKKLEIDDDLKLRAFWDKRISQEVIGDALCEEFKGYVFRITGCCDTQ*FP 184
Query: 61 MKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGEND 120
MK GVLTPGRVRLLLHRGTPC+RG GR GER RKSVRGCIVSP LSVLNLVI KK END
Sbjct: 185 MKHGVLTPGRVRLLLHRGTPCYRGSGRLYGERMRKSVRGCIVSPHLSVLNLVIAKKSEND 364
Query: 121 LPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFT 165
LPGLTDVEKP MR P+RA ++R LFNLS +DDVR+ V+ RT+T
Sbjct: 365 LPGLTDVEKPTMRRPERAYRLRNLFNLSNEDDVRENVDILPRTWT 499
>BG352914 homologue to SP|Q9M3V8|RS6_A 40S ribosomal protein S6. [Garden
asparagus] {Asparagus officinalis}, partial (31%)
Length = 481
Score = 162 bits (409), Expect = 1e-40
Identities = 75/78 (96%), Positives = 78/78 (99%)
Frame = +3
Query: 1 FNVANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFP 60
FN+A+PTTGCQKKLEIDDDQKLRAFWDKRISQEV+GDALGEEFKGYVFKITGGCDKQGFP
Sbjct: 30 FNIASPTTGCQKKLEIDDDQKLRAFWDKRISQEVSGDALGEEFKGYVFKITGGCDKQGFP 209
Query: 61 MKQGVLTPGRVRLLLHRG 78
MKQGVLTPGRVRLLLHRG
Sbjct: 210 MKQGVLTPGRVRLLLHRG 263
>BG239336
Length = 386
Score = 159 bits (403), Expect = 5e-40
Identities = 75/78 (96%), Positives = 76/78 (97%)
Frame = +1
Query: 1 FNVANPTTGCQKKLEIDDDQKLRAFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFP 60
FN+ANPTTGCQKKLEIDDD KLRAFWDKRISQEV GDALGEEFKGYVFKITGGCDKQGFP
Sbjct: 25 FNIANPTTGCQKKLEIDDDLKLRAFWDKRISQEVVGDALGEEFKGYVFKITGGCDKQGFP 204
Query: 61 MKQGVLTPGRVRLLLHRG 78
MKQGVLTPGRVRLLLHRG
Sbjct: 205 MKQGVLTPGRVRLLLHRG 258
>BE807460 similar to GP|9931636|gb| ribosomal protein s6 RPS6-2 {Zea mays},
partial (47%)
Length = 400
Score = 116 bits (291), Expect = 5e-27
Identities = 57/65 (87%), Positives = 61/65 (93%)
Frame = +2
Query: 108 VLNLVIVKKGENDLPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTK 167
VLNL IVKKGE+DLPGLTDVEKPRMRGPKRASKIRKLFNLSK+DDVRKYVNTYRRTFTT
Sbjct: 2 VLNLGIVKKGESDLPGLTDVEKPRMRGPKRASKIRKLFNLSKEDDVRKYVNTYRRTFTTN 181
Query: 168 DGAKL 172
+ K+
Sbjct: 182 NWVKV 196
>TC203351 homologue to UP|Q6SPR3 (Q6SPR3) Ribosomal protein S6, partial (46%)
Length = 564
Score = 79.7 bits (195), Expect = 6e-16
Identities = 37/43 (86%), Positives = 41/43 (95%)
Frame = +1
Query: 132 MRGPKRASKIRKLFNLSKDDDVRKYVNTYRRTFTTKDGAKLPR 174
MRGPKRASKIRKLFNLSK+DDVRKYVNTYRRTFTTK+G K+ +
Sbjct: 1 MRGPKRASKIRKLFNLSKEDDVRKYVNTYRRTFTTKNGKKVSK 129
>AI856824 similar to SP|Q9M3V8|RS6_A 40S ribosomal protein S6. [Garden
asparagus] {Asparagus officinalis}, partial (17%)
Length = 151
Score = 68.9 bits (167), Expect = 1e-12
Identities = 33/50 (66%), Positives = 38/50 (76%)
Frame = +2
Query: 24 AFWDKRISQEVNGDALGEEFKGYVFKITGGCDKQGFPMKQGVLTPGRVRL 73
+FWDKRI+ E G ALGE+ KGYVFKI G DKQG PMKQGVL PG + +
Sbjct: 2 SFWDKRITHEDLGAALGEDPKGYVFKIYRGRDKQGLPMKQGVLNPGSMHV 151
>BI785648 homologue to GP|22296425|dbj 40S ribosomal protein S6 {Oryza
sativa (japonica cultivar-group)}, partial (9%)
Length = 422
Score = 48.1 bits (113), Expect = 2e-06
Identities = 21/25 (84%), Positives = 23/25 (92%)
Frame = +1
Query: 1 FNVANPTTGCQKKLEIDDDQKLRAF 25
FN+ANPTTGCQKKLEIDDD KL +F
Sbjct: 19 FNIANPTTGCQKKLEIDDDLKL*SF 93
>CA938560 homologue to GP|22296425|dbj 40S ribosomal protein S6 {Oryza
sativa (japonica cultivar-group)}, partial (9%)
Length = 444
Score = 47.8 bits (112), Expect = 3e-06
Identities = 20/22 (90%), Positives = 22/22 (99%)
Frame = +2
Query: 1 FNVANPTTGCQKKLEIDDDQKL 22
FN+A+PTTGCQKKLEIDDDQKL
Sbjct: 77 FNIASPTTGCQKKLEIDDDQKL 142
>BF070100
Length = 412
Score = 30.4 bits (67), Expect = 0.45
Identities = 22/61 (36%), Positives = 29/61 (47%), Gaps = 4/61 (6%)
Frame = +2
Query: 60 PMKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKS----VRGCIVSPDLSVLNLVIVK 115
P + G P R ++ RGT G GRR +RRR+ RGC V LS L V+
Sbjct: 89 PRRPGRTKPKRNIIITARGT----GRGRRGEQRRRRPRVIPARGCSVRIGLSPFKLSRVR 256
Query: 116 K 116
+
Sbjct: 257 R 259
>TC226462 homologue to UP|PCNA_PEA (O82134) Proliferating cell nuclear
antigen, complete
Length = 1202
Score = 29.6 bits (65), Expect = 0.77
Identities = 17/49 (34%), Positives = 23/49 (46%)
Frame = +1
Query: 56 KQGFPMKQGVLTPGRVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSP 104
++GF ++QG +RLLLHR P G R R +RG P
Sbjct: 151 EEGFGIRQGARERRELRLLLHRVLPPGHGLQPRRPRRSPPPIRGLRALP 297
>TC203554 homologue to UP|RL38_ARATH (O22860) 60S ribosomal protein L38,
complete
Length = 1822
Score = 28.9 bits (63), Expect = 1.3
Identities = 15/45 (33%), Positives = 23/45 (50%)
Frame = -1
Query: 119 NDLPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNTYRRT 163
ND+P D +PR+ P+R KI L +L + +R + RT
Sbjct: 1024 NDIPAPLDFHRPRILPPRRQKKILNLVDLLRHCGLRVVSDGGERT 890
>TC235033
Length = 404
Score = 28.9 bits (63), Expect = 1.3
Identities = 21/45 (46%), Positives = 23/45 (50%), Gaps = 2/45 (4%)
Frame = -2
Query: 52 GGCDKQGFPMKQGVLTP--GRVRLLLHRGTPCFRGHGRRNGERRR 94
GGC G P+ G P GR R RG C+RG GRR RRR
Sbjct: 340 GGCGGWGTPIGSGGWRPSVGRRR---ERGACCWRGGGRR---RRR 224
>AW597075 similar to PIR|T47436|T474 protein kinase-like protein -
Arabidopsis thaliana, partial (8%)
Length = 380
Score = 28.1 bits (61), Expect = 2.2
Identities = 18/39 (46%), Positives = 20/39 (51%), Gaps = 2/39 (5%)
Frame = -2
Query: 52 GGCDKQGFPMKQGVLTP--GRVRLLLHRGTPCFRGHGRR 88
GGC G P+ G P GR R RG C+RG GRR
Sbjct: 316 GGCGGWGSPIGSGGWRPSGGRRRA---RGACCWRGGGRR 209
>TC223944 similar to GB|AAP68218.1|31711724|BT008779 At5g51910 {Arabidopsis
thaliana;} , partial (8%)
Length = 431
Score = 27.7 bits (60), Expect = 2.9
Identities = 25/72 (34%), Positives = 34/72 (46%), Gaps = 6/72 (8%)
Frame = -1
Query: 70 RVRLLLHRGTPCFRGHGRRNGERRRKSVRGCIVSPDLSVLNLVIVKKGENDL---PGLTD 126
R R G+ R H R + RR +RGC V V+ +++ +G N+ PGL D
Sbjct: 218 RSRTRARTGSNSHRAHSRTHSGRRVNGIRGCTV---*RVVVVMLHLRGGNEPRNGPGL-D 51
Query: 127 VEKP---RMRGP 135
VEK R GP
Sbjct: 50 VEKRGRCRRNGP 15
>TC226950 weakly similar to UP|Q7XCK3 (Q7XCK3) Mucin-like protein, partial
(49%)
Length = 1290
Score = 26.9 bits (58), Expect = 5.0
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +1
Query: 29 RISQEVNGDALGEEFKGYVFKITGGCD 55
R+S E + L +EF+G FK+T G D
Sbjct: 439 RVSSEKEIELLVDEFEGLKFKLTDGVD 519
>TC208776 similar to UP|SGPL_ARATH (Q9C509) Sphingosine-1-phosphate lyase
(SP-lyase) (SPL) (Sphingosine-1-phosphate aldolase) ,
partial (34%)
Length = 716
Score = 26.9 bits (58), Expect = 5.0
Identities = 11/17 (64%), Positives = 13/17 (75%)
Frame = -3
Query: 83 RGHGRRNGERRRKSVRG 99
RG GRR+GER R+ RG
Sbjct: 129 RGGGRRSGERERRGERG 79
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.141 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,103,209
Number of Sequences: 63676
Number of extensions: 89216
Number of successful extensions: 488
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 488
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 488
length of query: 176
length of database: 12,639,632
effective HSP length: 91
effective length of query: 85
effective length of database: 6,845,116
effective search space: 581834860
effective search space used: 581834860
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146343.12