BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146333.5 + phase: 0
(501 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC222199 108 6e-24
TC216808 62 3e-21
BG508280 58 1e-08
BI317622 54 2e-07
BE611176 47 3e-05
BI315644 39 0.004
TC221593 similar to UP|Q6NN02 (Q6NN02) At4g14450, partial (30%) 32 0.86
TC218751 similar to UP|Q6W6U5 (Q6W6U5) Olfactory receptor family... 31 1.1
TC205325 similar to UP|Q8L7S4 (Q8L7S4) At1g68060/T23K23_9, parti... 30 2.5
BI426606 30 2.5
CD391123 30 2.5
TC234562 similar to UP|HT22_ARATH (P46604) Homeobox-leucine zipp... 29 5.6
TC234236 similar to UP|Q9LW21 (Q9LW21) Arabidopsis thaliana geno... 29 5.6
TC222651 homologue to UP|Q9B8W1 (Q9B8W1) NADH dehydrogenase subu... 28 7.3
TC221337 similar to UP|Q9SD57 (Q9SD57) RNA-binding protein-like ... 28 7.3
TC231195 weakly similar to UP|Q94JR2 (Q94JR2) AT5g45310/K9E15_9,... 28 9.6
>TC222199
Length = 691
Score = 108 bits (270), Expect = 6e-24
Identities = 72/228 (31%), Positives = 114/228 (49%), Gaps = 22/228 (9%)
Frame = +2
Query: 91 DFQLVPTLEAYSHLLDSPIAEKTPFTGPGASLTPLVIAKDLYLKTSDVSKHLTTKSHIRG 150
DFQLVPT+E + +L P+ E+ P+ G + I + + + T++ I G
Sbjct: 5 DFQLVPTIEEFEEILGCPLGERKPYLSSGCLPSLSRIPTMVKDSARGLDRVKQTRNGIAG 184
Query: 151 FTSKYLLEQANLETTCQDALEA--ILALLVYGLILFPNLDNFVDMNAIEIF----HSR-N 203
KYL ++A D + +LALL++G++LFPN+D VD+ AI+ F HS+ +
Sbjct: 185 LPRKYLEDKARGIANQGDWVPFMDVLALLIFGVVLFPNVDGLVDLAAIDAFLAYHHSKES 364
Query: 204 PVPTLLADMYHAIHDRTLKGRVYILCCVPLLYRWFISHL---------PSSFHDNSE--- 251
PV +LAD++ + R K I+CC+P L W +SHL P H +
Sbjct: 365 PVVAVLADLFDTFNRRYEKSSARIICCLPALCVWLVSHLFQQDTRRPCPLLSHRSCTEKR 544
Query: 252 --DWSYSQRMMALSPNEVVWITPAAQVKE-IITGCGDFLNVPLLGTRG 296
DW Q + + + W + KE ++ CG + N+PL+GTRG
Sbjct: 545 RIDW--DQLLAGIGGRTISWFPRWKEGKEGVLFSCGRYPNIPLVGTRG 682
>TC216808
Length = 1144
Score = 61.6 bits (148), Expect(2) = 3e-21
Identities = 37/138 (26%), Positives = 68/138 (48%), Gaps = 2/138 (1%)
Frame = +2
Query: 25 KKTKSYKFKEVDLVSLRDLA--LKVKNQTGFRLRYGGLLTILRTNVEEKLVHTLVQFYDP 82
K+ K K +++ SL +L + + FR YG + + R V + + +L Q+YD
Sbjct: 146 KRFYQVKIKSLEVTSLGELGQLMGQLQR*AFRKAYGKIWDLARVEVSMEPIASLSQYYDQ 325
Query: 83 SFRCFTFPDFQLVPTLEAYSHLLDSPIAEKTPFTGPGASLTPLVIAKDLYLKTSDVSKHL 142
RCFTF DFQLVPT++ + + + P+ G + +AK + + ++++
Sbjct: 326 PLRCFTFEDFQLVPTVKELEEIRGCQLGGRKPYLFSGFYPSTTRVAKVVKISAQELNRVK 505
Query: 143 TTKSHIRGFTSKYLLEQA 160
++ + G K L E+A
Sbjct: 506 QNRNGVVGVPRKCLEERA 559
Score = 58.5 bits (140), Expect(2) = 3e-21
Identities = 32/75 (42%), Positives = 45/75 (59%), Gaps = 5/75 (6%)
Frame = +3
Query: 173 ILALLVYGLILFPNLDNFVDMNAIEIF----HSR-NPVPTLLADMYHAIHDRTLKGRVYI 227
ILALL++G++LFPN++ VD+ AI+ F H + +PV +LA MY K I
Sbjct: 603 ILALLIFGVVLFPNMEGLVDLAAIDAFLAFHHGKESPVVAILA-MYDTFDRGCEKSSARI 779
Query: 228 LCCVPLLYRWFISHL 242
+CC LY W +SHL
Sbjct: 780 VCCALALYVWLVSHL 824
>BG508280
Length = 418
Score = 57.8 bits (138), Expect = 1e-08
Identities = 39/111 (35%), Positives = 61/111 (54%), Gaps = 4/111 (3%)
Frame = +1
Query: 279 IITGCGDFLNVPLLGTRGGINYNPELAMRQFGFPMKTKPINLATSPEF---FYYSNAPTG 335
++ CG F NVPL+GTRG I+YNP LA++Q G+PM P+ +P F +N T
Sbjct: 70 VLISCGGFPNVPLMGTRGCISYNPVLAIKQLGYPM*GVPLEDELAPVISRGFNKTNVETL 249
Query: 336 QREAFTRAWSKVRWKSVKHLGVRSGIAHEAYTQWVINRAEEIGLPY-PAMR 385
Q+ +AW ++ K + G +G Y +W+ +A GL + P++R
Sbjct: 250 QK--VRKAWEVLQKKDKELRGSNNG-PIGGYRKWL--KAHVQGLDWLPSLR 387
>BI317622
Length = 370
Score = 53.9 bits (128), Expect = 2e-07
Identities = 38/125 (30%), Positives = 53/125 (42%), Gaps = 20/125 (16%)
Frame = -2
Query: 188 DNFVDMNAIEIF-----HSRNPVPTLLADMYHAIHDRTLKGRVYILCCVPLLYRWFISHL 242
D VD+ AI F + P+ +LAD+Y + R K I+CC P LY W +SHL
Sbjct: 369 DGLVDLAAIGAFLAYHDYKEIPIVAMLADLYDTFNRRCEKSSTRIVCCTPALYVWLVSHL 190
Query: 243 ---------PSSFH-----DNSEDWSYSQRMMALSPNEVVWITPAAQVK-EIITGCGDFL 287
P H E+W Q + V W + + ++ CG FL
Sbjct: 189 FRQEVRHACPLESHCSCTEKGGENW--GQLLANKEGASVNWFPRWKEGRTRVLISCGVFL 16
Query: 288 NVPLL 292
NVPL+
Sbjct: 15 NVPLM 1
>BE611176
Length = 424
Score = 46.6 bits (109), Expect = 3e-05
Identities = 28/97 (28%), Positives = 44/97 (44%), Gaps = 2/97 (2%)
Frame = -2
Query: 25 KKTKSYKFKEVDLVSLRDLA--LKVKNQTGFRLRYGGLLTILRTNVEEKLVHTLVQFYDP 82
K+ K K +D+ S+++L + FR Y +L + + + + +L Q+YD
Sbjct: 360 KRFYQVKIKGLDITSIKELGRLMGPLQMQAFRKAYEKILDLTIAEISTEAIASLTQYYD* 181
Query: 83 SFRCFTFPDFQLVPTLEAYSHLLDSPIAEKTPFTGPG 119
RCFTF DFQL L+ D + E PG
Sbjct: 180 PLRCFTFGDFQLDRPLKNLRRF*DVLLGEGNRIFSPG 70
>BI315644
Length = 421
Score = 39.3 bits (90), Expect = 0.004
Identities = 17/31 (54%), Positives = 23/31 (73%)
Frame = +3
Query: 294 TRGGINYNPELAMRQFGFPMKTKPINLATSP 324
TRG INYNP LA+RQ G+PM+ P + + +P
Sbjct: 276 TRGCINYNPVLAIRQLGYPMRGVPSDESITP 368
>TC221593 similar to UP|Q6NN02 (Q6NN02) At4g14450, partial (30%)
Length = 514
Score = 31.6 bits (70), Expect = 0.86
Identities = 18/51 (35%), Positives = 27/51 (52%)
Frame = +1
Query: 371 INRAEEIGLPYPAMRHVAASAPSIPLPLPPTTQEMYQEHLAMESREKQMWK 421
INRA E + P + +A+S P PLPLPP ++ Q+ E + W+
Sbjct: 193 INRALEWNVAIPLLSPLASSPP--PLPLPPQKEDPPQQQRPTEKVVFKKWQ 339
>TC218751 similar to UP|Q6W6U5 (Q6W6U5) Olfactory receptor family 8 subfamily
S (Fragment), partial (7%)
Length = 988
Score = 31.2 bits (69), Expect = 1.1
Identities = 20/60 (33%), Positives = 33/60 (54%), Gaps = 4/60 (6%)
Frame = +2
Query: 407 QEHLAMESREKQ----MWKARYNEAENLIMTLDGKDEQKTHENLMLKKELVKVRRELEEK 462
Q +LA ES E + + ++ EAE I L+ + + T E +LKKEL ++R++ K
Sbjct: 491 QSNLANESEELKSKLSVMDSKLREAEVTITKLNEERRRNTQEKDLLKKELEVLKRQINTK 670
>TC205325 similar to UP|Q8L7S4 (Q8L7S4) At1g68060/T23K23_9, partial (26%)
Length = 1204
Score = 30.0 bits (66), Expect = 2.5
Identities = 17/68 (25%), Positives = 31/68 (45%)
Frame = +3
Query: 399 PPTTQEMYQEHLAMESREKQMWKARYNEAENLIMTLDGKDEQKTHENLMLKKELVKVRRE 458
PP+ + E WK ++ N + T+D +D +L+KE++ +R+
Sbjct: 267 PPSYSFNQSSEGTKDGEENDKWKGNSDDKPNDLPTVDTEDSVPGVLYDLLQKEVLALRKA 446
Query: 459 LEEKDELL 466
EKD+ L
Sbjct: 447 GHEKDQSL 470
>BI426606
Length = 357
Score = 30.0 bits (66), Expect = 2.5
Identities = 21/78 (26%), Positives = 39/78 (49%), Gaps = 7/78 (8%)
Frame = +3
Query: 396 LPLPPTTQEMYQEHLAMESREKQMWKAR-------YNEAENLIMTLDGKDEQKTHENLML 448
LPLPP + ++ H + R ++ R ++EAENL M +DG + ++
Sbjct: 93 LPLPPLLRPLHPHHKLLLPRHPKVQLLRHRLHLRLFSEAENLHMNIDG-------DRFLV 251
Query: 449 KKELVKVRRELEEKDELL 466
EL +R + +KD+++
Sbjct: 252 SNEL--LRSKPCDKDDII 299
>CD391123
Length = 613
Score = 30.0 bits (66), Expect = 2.5
Identities = 20/60 (33%), Positives = 32/60 (53%), Gaps = 4/60 (6%)
Frame = -3
Query: 407 QEHLAMESREKQ----MWKARYNEAENLIMTLDGKDEQKTHENLMLKKELVKVRRELEEK 462
Q +LA ES E + + ++ EAE I L+ + + T E +LKKEL ++R + K
Sbjct: 470 QSNLAHESEELKSKLSVMDSKLREAEVTITKLNEERRRNTQEKDLLKKELEVLKRPIHTK 291
>TC234562 similar to UP|HT22_ARATH (P46604) Homeobox-leucine zipper protein
HAT22 (HD-ZIP protein 22), partial (33%)
Length = 585
Score = 28.9 bits (63), Expect = 5.6
Identities = 19/67 (28%), Positives = 33/67 (48%), Gaps = 6/67 (8%)
Frame = +3
Query: 398 LPPTTQEMYQEHLAMESREKQMW------KARYNEAENLIMTLDGKDEQKTHENLMLKKE 451
L P ++ E L ++ R+ ++W + + + E L E+ T ENL LKKE
Sbjct: 366 LNPAQKQALAEQLNLKHRQVEVWFQNRRARTKLKQTEVDCEFLKKCCEKLTDENLRLKKE 545
Query: 452 LVKVRRE 458
L ++R +
Sbjct: 546 LQELRAQ 566
>TC234236 similar to UP|Q9LW21 (Q9LW21) Arabidopsis thaliana genomic DNA,
chromosome 3, P1 clone: MDJ14, partial (4%)
Length = 586
Score = 28.9 bits (63), Expect = 5.6
Identities = 16/39 (41%), Positives = 19/39 (48%)
Frame = -1
Query: 8 IHRRIGQIKNFVNITGMKKTKSYKFKEVDLVSLRDLALK 46
I R QIKN NIT K +YK ++ RDL K
Sbjct: 439 IMRAETQIKNVTNITNEKNKNAYKHSTAYMLGRRDLESK 323
>TC222651 homologue to UP|Q9B8W1 (Q9B8W1) NADH dehydrogenase subunit 6,
partial (8%)
Length = 1006
Score = 28.5 bits (62), Expect = 7.3
Identities = 21/85 (24%), Positives = 42/85 (48%), Gaps = 10/85 (11%)
Frame = -1
Query: 235 YRWFISHLPSSFHDNSEDWSYSQRMMA-LSPNEVVWIT---------PAAQVKEIITGCG 284
Y W I +L FH N+E+ +YS +++ ++++I+ +K I+
Sbjct: 628 YLWLIKYL*KDFHINNENQTYSYKILIYFLSTKLIFISIK*ILKTNLKIIYIK*ILKMNL 449
Query: 285 DFLNVPLLGTRGGINYNPELAMRQF 309
+ L +PL G N+N ++++R F
Sbjct: 448 ETLLLPL-----GSNFNNDISLRDF 389
>TC221337 similar to UP|Q9SD57 (Q9SD57) RNA-binding protein-like protein,
partial (77%)
Length = 1151
Score = 28.5 bits (62), Expect = 7.3
Identities = 19/65 (29%), Positives = 34/65 (52%), Gaps = 1/65 (1%)
Frame = +1
Query: 140 KHL-TTKSHIRGFTSKYLLEQANLETTCQDALEAILALLVYGLILFPNLDNFVDMNAIEI 198
KHL TK ++ TSK L + ++E LE + L + ++ PN ++ + M E
Sbjct: 667 KHLCATKYKLKDLTSKGNLSEIDMERE----LECGICLEINSKVVLPNCNHSMCMKCYED 834
Query: 199 FHSRN 203
+H+R+
Sbjct: 835 WHARS 849
>TC231195 weakly similar to UP|Q94JR2 (Q94JR2) AT5g45310/K9E15_9, partial
(16%)
Length = 634
Score = 28.1 bits (61), Expect = 9.6
Identities = 18/51 (35%), Positives = 26/51 (50%), Gaps = 7/51 (13%)
Frame = +3
Query: 407 QEHLAMESREKQMWKARYNEAENL-------IMTLDGKDEQKTHENLMLKK 450
QEH M +E++M + E E I L+GK +T+ENL LK+
Sbjct: 360 QEHFQMAVKERKMMEILLAELEEEHDMAIANIEKLEGKLRDQTNENLRLKE 512
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.136 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,788,641
Number of Sequences: 63676
Number of extensions: 324645
Number of successful extensions: 1951
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 1935
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1943
length of query: 501
length of database: 12,639,632
effective HSP length: 101
effective length of query: 400
effective length of database: 6,208,356
effective search space: 2483342400
effective search space used: 2483342400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146333.5