BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146329.5 + phase: 0
(639 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC205643 homologue to UP|FTHS_SPIOL (P28723) Formate--tetrahydro... 669 0.0
TC205644 homologue to UP|FTHS_SPIOL (P28723) Formate--tetrahydro... 551 e-157
TC205642 similar to UP|FTHS_SPIOL (P28723) Formate--tetrahydrofo... 350 1e-96
TC209908 similar to UP|Q945B7 (Q945B7) Leucine zipper-containing... 94 1e-19
TC214360 similar to UP|Q9MBF3 (Q9MBF3) Glycine-rich RNA-binding ... 38 0.012
TC215068 similar to UP|FER1_LYCES (Q43517) Ferredoxin I, chlorop... 30 2.5
BE800973 similar to SP|P12468|RBS4 Ribulose bisphosphate carboxy... 30 3.3
TC218424 weakly similar to UP|Q6NLA4 (Q6NLA4) RH74182p, partial ... 30 4.3
TC214419 UP|PRP3_SOYBN (P15642) Repetitive proline-rich cell wal... 30 4.3
TC218750 29 5.6
AW733596 29 7.4
TC222171 weakly similar to UP|Q6ZLF2 (Q6ZLF2) 1-phosphatidylinos... 28 9.6
TC209599 similar to UP|P93698 (P93698) CPRD46 protein , partial ... 28 9.6
TC232195 homologue to UP|Q94F14 (Q94F14) Glutamate dehydrogenase... 28 9.6
>TC205643 homologue to UP|FTHS_SPIOL (P28723) Formate--tetrahydrofolate
ligase (Formyltetrahydrofolate synthetase) (FHS)
(FTHFS) , partial (56%)
Length = 1338
Score = 669 bits (1727), Expect = 0.0
Identities = 329/355 (92%), Positives = 347/355 (97%)
Frame = +2
Query: 285 DMRERLGKMVIGNSKSGDPVTADDLGIGGALTVLMKDAIHPTLMQTLEGTPVLVHAGPFA 344
DMRERLGKMVIGNSKSGDPVTADDLG+GGALTVLMKDAIHPTLMQTLEGTPVLVHAGPFA
Sbjct: 5 DMRERLGKMVIGNSKSGDPVTADDLGVGGALTVLMKDAIHPTLMQTLEGTPVLVHAGPFA 184
Query: 345 NIAHGNSSIVADKIALKLVGPGGFVVTEAGFGADIGTEKFMNIKCRYSGLTPQCAIIVAT 404
NIAHGNSSIVADKIALKLVGPGGFVVTEAGFGADIG EKFMNIKCRYSGLTPQCAIIVAT
Sbjct: 185 NIAHGNSSIVADKIALKLVGPGGFVVTEAGFGADIGAEKFMNIKCRYSGLTPQCAIIVAT 364
Query: 405 IRALKMHGGGPAVVAGKPLDHAYLTENVALVEAGCANLARHILNSKAYGANVIVAINKFS 464
IRALKMHGGGPAVVAGKPLDHAYLTENVALVEAGC N+ARHI N+K+YG NV+VAINKFS
Sbjct: 365 IRALKMHGGGPAVVAGKPLDHAYLTENVALVEAGCVNMARHISNTKSYGVNVVVAINKFS 544
Query: 465 TDTDAELNAVKKAALDAGAYDAVICSHHAHGGRGAVDLGIAVQKACENATQPLKFLYPLD 524
TDT+AELNAV+ AAL AGAYDAVIC+HHA+GG+GAVDLGIAVQKACEN TQPLKFLYP+D
Sbjct: 545 TDTEAELNAVQSAALAAGAYDAVICTHHANGGKGAVDLGIAVQKACENVTQPLKFLYPVD 724
Query: 525 IGIKEKIEAIAKSYGASGVEYSEQAEKKIELYTKQGFSGLPICMAKTQYSFSDNAAAKGA 584
+ IKEKIEAIAKSYGASGVEYSEQAEK+IE+Y+KQGFSGLPICMAKTQYSFSDNAAAKGA
Sbjct: 725 LSIKEKIEAIAKSYGASGVEYSEQAEKQIEMYSKQGFSGLPICMAKTQYSFSDNAAAKGA 904
Query: 585 PTGFILPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTTTGQVIGLS 639
P+GF+LPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTTTG+VIGLS
Sbjct: 905 PSGFVLPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTTTGKVIGLS 1069
>TC205644 homologue to UP|FTHS_SPIOL (P28723) Formate--tetrahydrofolate
ligase (Formyltetrahydrofolate synthetase) (FHS)
(FTHFS) , partial (46%)
Length = 1287
Score = 551 bits (1421), Expect = e-157
Identities = 273/295 (92%), Positives = 286/295 (96%)
Frame = +1
Query: 5 SSSSVMRKLDVVSPVPSDIDIANSVQPIHISEIANHLNLTPNHYDLYGKYKAKVLLSALD 64
SSS+ +RKL VVSPVP+DIDIANSV+P+HIS+IA LNL+PNHYDLYGKYKAKVLLS LD
Sbjct: 163 SSSTTVRKLQVVSPVPADIDIANSVEPVHISQIAKDLNLSPNHYDLYGKYKAKVLLSVLD 342
Query: 65 ELQESKDGYYVVVGGITPTPLGEGKSTTTVGLCQALGAFLDKKVVTCIRQPSQGPTFGIK 124
ELQ S+DGYYVVVGGITPTPLGEGKSTTTVGLCQALGAFLDKKVVTC+RQPSQGPTFGIK
Sbjct: 343 ELQGSEDGYYVVVGGITPTPLGEGKSTTTVGLCQALGAFLDKKVVTCLRQPSQGPTFGIK 522
Query: 125 GGAAGGGYSQVIPMDEFNLHLTGDIHAITAANNLLAAAIDTRIFHESTQSDKALFNRLCP 184
GGAAGGGYSQVIPMDEFNLHLTGDIHAITAANNLLAAAIDTRIFHESTQSDKALFNRLCP
Sbjct: 523 GGAAGGGYSQVIPMDEFNLHLTGDIHAITAANNLLAAAIDTRIFHESTQSDKALFNRLCP 702
Query: 185 PNKEGKRSFSDVMFRRLKKFGISKTNPDDLTPEEVNKFARLDIDPDSITWRRVMDINDRF 244
PNKEGKRSFSDVMFRRLKK GISKTNPDDLTPEEVNKFARLDIDP+SITWRRVMDINDRF
Sbjct: 703 PNKEGKRSFSDVMFRRLKKLGISKTNPDDLTPEEVNKFARLDIDPNSITWRRVMDINDRF 882
Query: 245 LRKITVGQGPDEKGMVRETAFDISVASEIMAVLALTTSLTDMRERLGKMVIGNSK 299
LRKIT+GQGPDEKGMVRET FDISVASEIMAVLALTTSL DMRERLGKMVIGN++
Sbjct: 883 LRKITIGQGPDEKGMVRETGFDISVASEIMAVLALTTSLADMRERLGKMVIGNTR 1047
>TC205642 similar to UP|FTHS_SPIOL (P28723) Formate--tetrahydrofolate ligase
(Formyltetrahydrofolate synthetase) (FHS) (FTHFS) ,
partial (32%)
Length = 772
Score = 350 bits (898), Expect = 1e-96
Identities = 170/201 (84%), Positives = 185/201 (91%)
Frame = +1
Query: 439 CANLARHILNSKAYGANVIVAINKFSTDTDAELNAVKKAALDAGAYDAVICSHHAHGGRG 498
C N ARHI N+K YG N AINKFSTDT+AELNAV+ AAL AGAYDAVIC+HHAHGG+
Sbjct: 1 CVNXARHISNTKXYGVNXXXAINKFSTDTEAELNAVQSAALAAGAYDAVICTHHAHGGKX 180
Query: 499 AVDLGIAVQKACENATQPLKFLYPLDIGIKEKIEAIAKSYGASGVEYSEQAEKKIELYTK 558
AVDLGIAVQKAC+N TQPLKFLYP+++ IKEKIEAIAKSYGA GVEYSEQAEK+IE+Y+K
Sbjct: 181 AVDLGIAVQKACKNVTQPLKFLYPVELSIKEKIEAIAKSYGARGVEYSEQAEKQIEMYSK 360
Query: 559 QGFSGLPICMAKTQYSFSDNAAAKGAPTGFILPIRDVRASIGAGFIYPLVGTMSTMPGLP 618
QGFSGLPICMAKTQYSFSDNAAAKGAP+GF+LPIRDVRASIGAGFIYPLVGTMSTMPGLP
Sbjct: 361 QGFSGLPICMAKTQYSFSDNAAAKGAPSGFVLPIRDVRASIGAGFIYPLVGTMSTMPGLP 540
Query: 619 TRPCFYDIDLDTTTGQVIGLS 639
TRPCFYDI LDTTT +VIGLS
Sbjct: 541 TRPCFYDIGLDTTTEKVIGLS 603
>TC209908 similar to UP|Q945B7 (Q945B7) Leucine zipper-containing protein,
partial (18%)
Length = 420
Score = 94.4 bits (233), Expect = 1e-19
Identities = 45/61 (73%), Positives = 53/61 (86%)
Frame = +1
Query: 428 LTENVALVEAGCANLARHILNSKAYGANVIVAINKFSTDTDAELNAVKKAALDAGAYDAV 487
LTENVAL+EAGC N+ARHI N+K+YG NV++A NKFSTDT+AELN + AAL AGAYDAV
Sbjct: 238 LTENVALLEAGCVNMARHISNTKSYGVNVVLAFNKFSTDTEAELNGDQSAALAAGAYDAV 417
Query: 488 I 488
I
Sbjct: 418 I 420
>TC214360 similar to UP|Q9MBF3 (Q9MBF3) Glycine-rich RNA-binding protein,
partial (44%)
Length = 630
Score = 38.1 bits (87), Expect = 0.012
Identities = 19/30 (63%), Positives = 22/30 (73%)
Frame = -3
Query: 546 SEQAEKKIELYTKQGFSGLPICMAKTQYSF 575
S QA K+IE+Y QGFSG+ I M KTQ SF
Sbjct: 499 SNQAAKQIEMYK*QGFSGISIWMTKTQ*SF 410
>TC215068 similar to UP|FER1_LYCES (Q43517) Ferredoxin I, chloroplast
precursor, partial (78%)
Length = 731
Score = 30.4 bits (67), Expect = 2.5
Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 4/46 (8%)
Frame = +3
Query: 89 KSTTTVGLCQAL--GAFLDKK-VVTCIRQ-PSQGPTFGIKGGAAGG 130
+ TT LC + +FL +K VVT ++ P+ FG+KGG GG
Sbjct: 90 RMATTAALCGTMMSSSFLRRKAVVTSLKAFPNANAVFGVKGGRGGG 227
>BE800973 similar to SP|P12468|RBS4 Ribulose bisphosphate carboxylase small
chain 4 chloroplast precursor (EC 4.1.1.39), partial
(57%)
Length = 325
Score = 30.0 bits (66), Expect = 3.3
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +2
Query: 600 GAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTTTG 633
GAG + P G + ++ GLPT+ +YDI TG
Sbjct: 50 GAGMVAPFTG-LKSLAGLPTKKTYYDITSLAYTG 148
>TC218424 weakly similar to UP|Q6NLA4 (Q6NLA4) RH74182p, partial (23%)
Length = 1034
Score = 29.6 bits (65), Expect = 4.3
Identities = 22/88 (25%), Positives = 40/88 (45%), Gaps = 2/88 (2%)
Frame = +2
Query: 205 GISKTNPDDLTPEEVNKFARLDIDPDSITWRRVMDI--NDRFLRKITVGQGPDEKGMVRE 262
G SK NP+ + + + +++ DP I++R ++D+ + R++ GQGPD R
Sbjct: 317 GGSKPNPEYRSLGDHAESVKVEYDPQLISFRELLDVFWSSHDPRQV-YGQGPDVGNQYRS 493
Query: 263 TAFDISVASEIMAVLALTTSLTDMRERL 290
F MA ++ T R +
Sbjct: 494 IIFVNGTEESRMAAVSKEQEQTRSRSSI 577
>TC214419 UP|PRP3_SOYBN (P15642) Repetitive proline-rich cell wall protein 3
precursor, partial (33%)
Length = 749
Score = 29.6 bits (65), Expect = 4.3
Identities = 14/22 (63%), Positives = 16/22 (72%)
Frame = -3
Query: 554 ELYTKQGFSGLPICMAKTQYSF 575
E+Y QGFSG+ I M KTQ SF
Sbjct: 744 EMYK*QGFSGISIWMTKTQ*SF 679
>TC218750
Length = 1421
Score = 29.3 bits (64), Expect = 5.6
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = +1
Query: 92 TTVGLCQALGAFLDKKVVTCIRQPSQGPTFGIKGGAAGGGYS 133
T V A G L+ +++T GP+FGI GG+ G G S
Sbjct: 517 TGVSSSNACGEVLNTEILTA--HARDGPSFGISGGSVGMGAS 636
>AW733596
Length = 410
Score = 28.9 bits (63), Expect = 7.4
Identities = 18/52 (34%), Positives = 27/52 (51%)
Frame = +1
Query: 20 PSDIDIANSVQPIHISEIANHLNLTPNHYDLYGKYKAKVLLSALDELQESKD 71
P +D N V P+H +ANHLN N DL G+ + L L+++ +D
Sbjct: 181 PRMLDYLNRV-PMHKGGLANHLN---NQMDLAGQLPGQTL*RGLEQILGRED 324
>TC222171 weakly similar to UP|Q6ZLF2 (Q6ZLF2)
1-phosphatidylinositol-3-phosphate 5-kinase-like,
partial (3%)
Length = 494
Score = 28.5 bits (62), Expect = 9.6
Identities = 25/83 (30%), Positives = 39/83 (46%), Gaps = 6/83 (7%)
Frame = +1
Query: 386 NIKCRYSGLTPQCAIIVATIRALKMHGGGPAVVAGKPLDHAYLTENVALVEAGCANLARH 445
N+ + +G P A VA +H +V K + Y T N+ +EAGC N ++
Sbjct: 25 NLSLKENGCLPPDAAAVA------VHSPVANIVTSKSNSNIY-TANIGGIEAGCTNYSKL 183
Query: 446 I---LNSKAYG---ANVIVAINK 462
+ L++K G ANV + NK
Sbjct: 184 LSKGLDTKWKGIPFANVFGSFNK 252
>TC209599 similar to UP|P93698 (P93698) CPRD46 protein , partial (20%)
Length = 706
Score = 28.5 bits (62), Expect = 9.6
Identities = 21/61 (34%), Positives = 31/61 (50%), Gaps = 4/61 (6%)
Frame = +1
Query: 88 GKSTTTVGLCQALGAFLDKKVVTCIRQPSQGPTFGIKGGA----AGGGYSQVIPMDEFNL 143
G T+ LC G F K+ C +Q + G FG + GA AGG Y++V+P+ +
Sbjct: 103 GCELRTILLC---GVF-PKQTHHCKKQHALGGPFGRRMGAVLGEAGGDYAEVLPVADSGH 270
Query: 144 H 144
H
Sbjct: 271 H 273
>TC232195 homologue to UP|Q94F14 (Q94F14) Glutamate dehydrogenase 2, partial
(93%)
Length = 732
Score = 28.5 bits (62), Expect = 9.6
Identities = 11/16 (68%), Positives = 12/16 (74%)
Frame = +3
Query: 409 KMHGGGPAVVAGKPLD 424
K HG PAVV GKP+D
Sbjct: 567 KFHGHSPAVVTGKPID 614
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.136 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,569,914
Number of Sequences: 63676
Number of extensions: 290769
Number of successful extensions: 1298
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 1292
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1298
length of query: 639
length of database: 12,639,632
effective HSP length: 103
effective length of query: 536
effective length of database: 6,081,004
effective search space: 3259418144
effective search space used: 3259418144
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)
Medicago: description of AC146329.5