BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145372.5 - phase: 0
(369 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC216144 UP|Q6PP99 (Q6PP99) Mitochondrial pyruvate dehydrogenase... 674 0.0
TC216145 similar to UP|Q6PP98 (Q6PP98) Mitochondrial pyruvate de... 239 2e-63
AW100899 similar to GP|4049632|gb| pyruvate dehydrogenase kinase... 231 3e-61
BM094512 homologue to GP|4049632|gb| pyruvate dehydrogenase kina... 178 3e-51
BG882912 33 0.27
TC216028 homologue to UP|Q9ZSQ0 (Q9ZSQ0) Ethylene response senso... 32 0.35
BQ610568 similar to GP|19911203|db glucosyltransferase-10 {Vigna... 30 2.3
TC216811 similar to UP|WR11_ARATH (Q9SV15) Probable WRKY transcr... 29 3.9
TC216027 homologue to UP|Q9ZSQ0 (Q9ZSQ0) Ethylene response senso... 29 3.9
TC221580 29 3.9
BQ740318 28 6.6
TC210435 similar to UP|Q39224 (Q39224) SRG1 protein (F6I1.30/F6I... 28 8.6
>TC216144 UP|Q6PP99 (Q6PP99) Mitochondrial pyruvate dehydrogenase kinase
isoform 1 , complete
Length = 1536
Score = 674 bits (1740), Expect = 0.0
Identities = 327/369 (88%), Positives = 353/369 (95%)
Frame = +2
Query: 1 MAAKKAIETFSKSLIEEVHKWGCFKQTGVSLRYMMDFGSKPTSKNLLISAQFLHKELAIR 60
MAAKKA ETFSKSLIEEV++WGC KQTGVSLRYMM+FGSKPT+KNLLISAQFLHKELAIR
Sbjct: 182 MAAKKACETFSKSLIEEVNRWGCLKQTGVSLRYMMEFGSKPTNKNLLISAQFLHKELAIR 361
Query: 61 IARRAVELERLPCDLSQKPAVLKVRDWYLDSFRDIRSFSEIKNMNDEKEFTEVIKAIKVR 120
IARRAVELE LP LSQKPAVLKVRDWY+DSFRD+R+F +IKN+NDE+EFTE+IKAIKVR
Sbjct: 362 IARRAVELENLPYGLSQKPAVLKVRDWYVDSFRDVRAFPDIKNVNDEREFTEMIKAIKVR 541
Query: 121 HNNVVPTMALGVQQLKKDVDPKIINQDFVEIHQFLDRFYMSRIGIRMLIGQHVELHSPNP 180
HNNVVPTMA+GVQQLKK +DPKI+ +D VEIHQFLDRFYMSRIGIRMLIGQHVELH+PNP
Sbjct: 542 HNNVVPTMAMGVQQLKKGMDPKIVYEDLVEIHQFLDRFYMSRIGIRMLIGQHVELHNPNP 721
Query: 181 PPHVVGYIHTRMSPVEVARNAVDDARSICSRQYGSAPDVHIYGDPDFTFPYVPAHLHLMV 240
PPHVVGYIHT+MSPVEVARNA +DARSIC R+YGSAPDVHIYGDP+FTFPYVPAHLHLMV
Sbjct: 722 PPHVVGYIHTKMSPVEVARNASEDARSICCREYGSAPDVHIYGDPNFTFPYVPAHLHLMV 901
Query: 241 FELVKNSLRAVEERYMNSDKDPPPIRIIVADGLEDVTIKISDEGGGIPRSGLRKIFTYLY 300
FELVKNSLRAV+ER+MNSDK PPIRIIVADG+EDVTIK+SDEGGGI RSGL KIFTYLY
Sbjct: 902 FELVKNSLRAVQERFMNSDKVAPPIRIIVADGIEDVTIKVSDEGGGIARSGLPKIFTYLY 1081
Query: 301 STARNPLDEHADLGVGDNVTMAGYGYGLPISRLYARYFGGDLQIISMENYGTDAYLHLSR 360
STARNPLDEH+DLG+GDNVTMAGYGYGLPISRLYARYFGGDLQIISME YGTDAYLHLSR
Sbjct: 1082STARNPLDEHSDLGIGDNVTMAGYGYGLPISRLYARYFGGDLQIISMEGYGTDAYLHLSR 1261
Query: 361 LGDSQEPLP 369
LGDSQEPLP
Sbjct: 1262LGDSQEPLP 1288
>TC216145 similar to UP|Q6PP98 (Q6PP98) Mitochondrial pyruvate dehydrogenase
kinase isoform 2 , partial (39%)
Length = 685
Score = 239 bits (609), Expect = 2e-63
Identities = 117/150 (78%), Positives = 130/150 (86%), Gaps = 3/150 (2%)
Frame = +1
Query: 223 GDPDFTFPYVPAHLHLMVFELVKNSLRAVEERYMNSDKDPPPIRIIVADGLEDVTIKISD 282
GD DFTF YVP HLHLMVFELVKNSLRAV+ER+M+SD+ PPIRII+ADG+EDVTIK+ D
Sbjct: 1 GDXDFTFXYVPXHLHLMVFELVKNSLRAVQERFMDSDEVAPPIRIIIADGIEDVTIKVXD 180
Query: 283 EGGGIPRSGLRKIFTYLYSTARNPLD---EHADLGVGDNVTMAGYGYGLPISRLYARYFG 339
EGGGIPRSGL +IFTY STA+N E +D+G +NVTMAGYGYGLPI RLYARYFG
Sbjct: 181 EGGGIPRSGLPRIFTYXXSTAKNSSSVEHEPSDIGTMENVTMAGYGYGLPICRLYARYFG 360
Query: 340 GDLQIISMENYGTDAYLHLSRLGDSQEPLP 369
GDLQ+ISME YGTDAYLHLSRLGDSQEPLP
Sbjct: 361 GDLQVISMEGYGTDAYLHLSRLGDSQEPLP 450
>AW100899 similar to GP|4049632|gb| pyruvate dehydrogenase kinase
{Arabidopsis thaliana}, partial (34%)
Length = 430
Score = 231 bits (590), Expect = 3e-61
Identities = 114/149 (76%), Positives = 129/149 (86%)
Frame = +1
Query: 125 VPTMALGVQQLKKDVDPKIINQDFVEIHQFLDRFYMSRIGIRMLIGQHVELHSPNPPPHV 184
VPTMALGVQQLK + +D EI +FLDRFYMSRIGIRMLIGQHVELH+PNPPP+
Sbjct: 1 VPTMALGVQQLKN------VFEDPDEIDEFLDRFYMSRIGIRMLIGQHVELHNPNPPPNC 162
Query: 185 VGYIHTRMSPVEVARNAVDDARSICSRQYGSAPDVHIYGDPDFTFPYVPAHLHLMVFELV 244
VGYIHT MSPV VARNA +DARS+C R+YGSA +V IYGDPDFTFPYVPAHLHLMVFELV
Sbjct: 163 VGYIHTNMSPVNVARNASEDARSMCYREYGSAAEVRIYGDPDFTFPYVPAHLHLMVFELV 342
Query: 245 KNSLRAVEERYMNSDKDPPPIRIIVADGL 273
KNSLRAV+ER+M+SD+ PPIRII+ADG+
Sbjct: 343 KNSLRAVQERFMDSDEVAPPIRIIIADGI 429
>BM094512 homologue to GP|4049632|gb| pyruvate dehydrogenase kinase
{Arabidopsis thaliana}, partial (30%)
Length = 426
Score = 178 bits (451), Expect(2) = 3e-51
Identities = 85/95 (89%), Positives = 91/95 (95%)
Frame = +1
Query: 250 AVEERYMNSDKDPPPIRIIVADGLEDVTIKISDEGGGIPRSGLRKIFTYLYSTARNPLDE 309
AV+ER+MNSDK PPIRIIVADG+EDVTIK+SDEGGGI RSGL KIFTYLYSTARNPLDE
Sbjct: 142 AVQERFMNSDKVAPPIRIIVADGIEDVTIKVSDEGGGIARSGLPKIFTYLYSTARNPLDE 321
Query: 310 HADLGVGDNVTMAGYGYGLPISRLYARYFGGDLQI 344
H+DLG+GDNVTMAGYGYGLPISRLYARYFGGDLQI
Sbjct: 322 HSDLGIGDNVTMAGYGYGLPISRLYARYFGGDLQI 426
Score = 42.0 bits (97), Expect(2) = 3e-51
Identities = 20/25 (80%), Positives = 21/25 (84%)
Frame = +2
Query: 224 DPDFTFPYVPAHLHLMVFELVKNSL 248
+P F YVPAHLHLMVFELVKNSL
Sbjct: 65 NPLFLSLYVPAHLHLMVFELVKNSL 139
>BG882912
Length = 412
Score = 32.7 bits (73), Expect = 0.27
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = +1
Query: 1 MAAKKAIETFSKSLIEE 17
MAAKKA ETFSKSLIEE
Sbjct: 361 MAAKKACETFSKSLIEE 411
>TC216028 homologue to UP|Q9ZSQ0 (Q9ZSQ0) Ethylene response sensor, partial
(78%)
Length = 1745
Score = 32.3 bits (72), Expect = 0.35
Identities = 30/135 (22%), Positives = 51/135 (37%), Gaps = 18/135 (13%)
Frame = +2
Query: 230 PYVPAH-------LHLMVFELVKNSLRAVEERYMN-----------SDKDPPPIRIIVAD 271
P +P H L + +V N+++ +E Y++ D PP +D
Sbjct: 914 PDLPTHAIGDEKRLTQTLLNVVGNAVKFTKEGYVSIRVSVAKPESLQDWRPPEFYPASSD 1093
Query: 272 GLEDVTIKISDEGGGIPRSGLRKIFTYLYSTARNPLDEHADLGVGDNVTMAGYGYGLPIS 331
G + +++ D G GIP + +FT + P +G G GL I
Sbjct: 1094 GHFYIRVQVKDSGCGIPPQEIPHLFTKFAQSRSGPAR-----------PSSGAGLGLAIC 1240
Query: 332 RLYARYFGGDLQIIS 346
+ + GG + I S
Sbjct: 1241 KRFVNLMGGHIWIES 1285
>BQ610568 similar to GP|19911203|db glucosyltransferase-10 {Vigna angularis},
partial (28%)
Length = 461
Score = 29.6 bits (65), Expect = 2.3
Identities = 27/104 (25%), Positives = 46/104 (43%), Gaps = 5/104 (4%)
Frame = +1
Query: 187 YIHTRMSPVEVARNAVDDARSICSRQYGSAPDVHIYGDPDFTFPYVPAHLHLMVFELVKN 246
Y H R+ + D + + S ++ + PD G P+ P V A H+ L +
Sbjct: 181 YTHKRL----LKSRGPDSIKGLPSFRFETIPD----GLPE---PLVDATQHIP--SLCDS 321
Query: 247 SLRAVEERYMN-----SDKDPPPIRIIVADGLEDVTIKISDEGG 285
+ R + N +D D PP+ IV+DG+ T+ ++E G
Sbjct: 322 TRRTCLPHFRNLLTKINDSDAPPVSCIVSDGVMSFTLDAAEELG 453
>TC216811 similar to UP|WR11_ARATH (Q9SV15) Probable WRKY transcription
factor 11 (WRKY DNA-binding protein 11), partial (29%)
Length = 686
Score = 28.9 bits (63), Expect = 3.9
Identities = 28/98 (28%), Positives = 38/98 (38%), Gaps = 14/98 (14%)
Frame = -1
Query: 172 HVELHSPNPPPHVVGYIHTRMSPVEVARNAV--DDARSICSRQYGSAPDVHI-----YGD 224
HV+ + PP + I R SP V N V ARS C +H+ YGD
Sbjct: 395 HVDSNPKPTPPEMFSCIECRCSPSYVTINIVGSSGARSTCFLAGHPLTLLHL*YPLGYGD 216
Query: 225 PDFTFPYVPAHLH-------LMVFELVKNSLRAVEERY 255
P + P LH +FEL+ +L R+
Sbjct: 215 P--LIGFCPYFLHEYSSGGISAIFELITGTLTVFFTRF 108
>TC216027 homologue to UP|Q9ZSQ0 (Q9ZSQ0) Ethylene response sensor, partial
(30%)
Length = 869
Score = 28.9 bits (63), Expect = 3.9
Identities = 29/135 (21%), Positives = 50/135 (36%), Gaps = 18/135 (13%)
Frame = +3
Query: 230 PYVPAH-------LHLMVFELVKNSLRAVEERYMN-----------SDKDPPPIRIIVAD 271
P +P H L + +V N+++ +E Y++ D PP +D
Sbjct: 3 PDLPTHAIGDEKRLTQTLLNVVGNAVKFTKEGYVSVRVSVAKPESSQDWRPPEFYPASSD 182
Query: 272 GLEDVTIKISDEGGGIPRSGLRKIFTYLYSTARNPLDEHADLGVGDNVTMAGYGYGLPIS 331
G + +++ D G GI + +FT + P +G G GL I
Sbjct: 183 GHFYIRVQVKDSGCGILPQDIPHLFTKFAQSRSGPAR-----------PSSGAGLGLAIC 329
Query: 332 RLYARYFGGDLQIIS 346
+ + GG + I S
Sbjct: 330 KRFVNLMGGHIWIES 374
>TC221580
Length = 526
Score = 28.9 bits (63), Expect = 3.9
Identities = 11/29 (37%), Positives = 18/29 (61%)
Frame = +2
Query: 166 RMLIGQHVELHSPNPPPHVVGYIHTRMSP 194
R ++G H L SP+PPPH + + ++P
Sbjct: 128 RQILGHHHFLFSPSPPPHPPPHPSSSLTP 214
>BQ740318
Length = 426
Score = 28.1 bits (61), Expect = 6.6
Identities = 22/75 (29%), Positives = 34/75 (45%)
Frame = +3
Query: 2 AAKKAIETFSKSLIEEVHKWGCFKQTGVSLRYMMDFGSKPTSKNLLISAQFLHKELAIRI 61
+ +K T++ ++ + K V LR M DFG + L+ISA L L +
Sbjct: 90 SGQKQSITWTMETLDRIRVLA*NKDLMVILRLMKDFGRRILPSQLVISAMEL-SNLM*AV 266
Query: 62 ARRAVELERLPCDLS 76
+VE+ CDLS
Sbjct: 267 LFLSVEIYGCACDLS 311
>TC210435 similar to UP|Q39224 (Q39224) SRG1 protein (F6I1.30/F6I1.30)
(At1g17020/F6I1.30), partial (22%)
Length = 466
Score = 27.7 bits (60), Expect = 8.6
Identities = 11/26 (42%), Positives = 18/26 (68%)
Frame = +1
Query: 240 VFELVKNSLRAVEERYMNSDKDPPPI 265
V ELVK + V ++Y++ ++DPP I
Sbjct: 55 VHELVKQPITKVPDQYLHPNQDPPDI 132
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.140 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,661,266
Number of Sequences: 63676
Number of extensions: 193530
Number of successful extensions: 1012
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 993
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1010
length of query: 369
length of database: 12,639,632
effective HSP length: 99
effective length of query: 270
effective length of database: 6,335,708
effective search space: 1710641160
effective search space used: 1710641160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC145372.5