BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145331.1 - phase: 0
(520 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC232849 224 9e-59
TC212853 117 2e-26
TC233610 72 8e-13
BE347362 33 0.31
TC214296 similar to UP|Q9VH98 (Q9VH98) CG8348-PA, partial (5%) 32 0.68
BE608619 31 1.2
TC226533 similar to GB|AAG53613.1|12407658|AF285832 eukaryotic i... 31 1.5
TC217238 30 2.6
TC228450 30 2.6
AW830717 29 5.8
TC206116 UP|CC48_SOYBN (P54774) Cell division cycle protein 48 h... 29 5.8
TC231751 homologue to GB|BAB08420.1|9757998|AB025622 cell divisi... 28 7.6
TC225681 homologue to UP|Q9SZD4 (Q9SZD4) 26S proteasome subunit ... 28 9.9
TC225683 homologue to UP|Q9SZD4 (Q9SZD4) 26S proteasome subunit ... 28 9.9
TC213495 28 9.9
>TC232849
Length = 408
Score = 224 bits (570), Expect = 9e-59
Identities = 112/136 (82%), Positives = 123/136 (90%)
Frame = +1
Query: 173 EERYKKEVQMKHILPDVADKFFLDREGEEKGANILCFDEIQTVDVFAIVALSGILSRLLS 232
EERYK+EVQMK+ILP VAD FFLD E EKGA+ILCFDEIQTVDVFAIVALSGILSRLLS
Sbjct: 1 EERYKQEVQMKNILPAVADMFFLDGEENEKGASILCFDEIQTVDVFAIVALSGILSRLLS 180
Query: 233 SGTIIVATSNRAPKDLNEANMVPEFFQNLLSNLEEHCEKVLVGSEIDYRRFIAQRSENRV 292
SGTIIVATSNRAPKDLNEA M E FQ L+S LEEHCEKVL+GSEIDYRRFIAQ+SEN+V
Sbjct: 181 SGTIIVATSNRAPKDLNEAGMQKEIFQKLVSKLEEHCEKVLIGSEIDYRRFIAQKSENQV 360
Query: 293 NYLWPIERETINKFEK 308
+Y WPIE+E +N+FEK
Sbjct: 361 HYFWPIEKEAMNEFEK 408
>TC212853
Length = 499
Score = 117 bits (292), Expect = 2e-26
Identities = 50/65 (76%), Positives = 60/65 (91%)
Frame = +2
Query: 292 VNYLWPIERETINKFEKKWQDATGRFGGKVISNTISVMFGRTLEVPESCEGVARFTFDYL 351
V+Y WPIE+E +N+FEKKW D TGRFGG++ISNTISVMFGRTLEVP+S +GVARFTF+YL
Sbjct: 80 VHYFWPIEKEAMNEFEKKWHDVTGRFGGRIISNTISVMFGRTLEVPQSFDGVARFTFEYL 259
Query: 352 CGRPL 356
CGRP+
Sbjct: 260 CGRPV 274
>TC233610
Length = 629
Score = 71.6 bits (174), Expect = 8e-13
Identities = 45/149 (30%), Positives = 71/149 (47%)
Frame = +1
Query: 342 GVARFTFDYLCGRPLGAADYIAVAENYHTVFISDIPMMSMRIRDKARRFITLIDELYNHH 401
G A F F+ +C RPLGAADY + + +HT+ + IP+ + + A RF+TL+D +Y +
Sbjct: 16 GCAYFPFEEICDRPLGAADYFGLFKKFHTLVLDGIPIFGLHNKSAAHRFVTLVDVMYENK 195
Query: 402 SCLCCLASSSIDELFQGTEEGTLFDLESFQFETEAEGSKLRRDVLAEGNVGSGGTPVGIT 461
+ L C A S +LF E +EA+ R + N S
Sbjct: 196 ARLLCTAEGSPKDLF-----------EKIVTVSEAKNLAPRTSSRSRKNDDS-------- 318
Query: 462 SILSGQEELFTFQRAVSRLIEMQTQLYLD 490
++ E F R +SRL E+ ++ YL+
Sbjct: 319 NLCVDNELGFAKDRTISRLTEINSKEYLE 405
>BE347362
Length = 448
Score = 33.1 bits (74), Expect = 0.31
Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = +1
Query: 92 LYIYGNVGSGKTMLMDMFYSATEGIVKHRR-RYHFHEAMLRINEHMHKTWKKQMEEKPLQ 150
++ YG G+GKT M EG +HR Y E + RI E H T K ++ L+
Sbjct: 166 IFAYGQTGTGKTFTM-------EGTPEHRGVNYRTLEELFRITEERHGTMKYELSVSMLE 324
>TC214296 similar to UP|Q9VH98 (Q9VH98) CG8348-PA, partial (5%)
Length = 1491
Score = 32.0 bits (71), Expect = 0.68
Identities = 21/81 (25%), Positives = 32/81 (38%)
Frame = +2
Query: 12 EKKRENERRRILMDEVEKQQNDKDWWKRLNNKITERWTNSRKRPENVDPGVGKWVSYLKR 71
E++RE ER R E E+++ + +R+ + + P P G VSY
Sbjct: 341 ERERERERERERERERERERERERERERITSHSISMYQQQGSDPTKQSPATGFPVSYSNS 520
Query: 72 EKKLDSLVGRRPTAPPAPKGL 92
+ P PP PK L
Sbjct: 521 TTYSTNEASYAPVPPPQPKPL 583
>BE608619
Length = 256
Score = 31.2 bits (69), Expect = 1.2
Identities = 15/59 (25%), Positives = 29/59 (48%)
Frame = +1
Query: 9 SNWEKKRENERRRILMDEVEKQQNDKDWWKRLNNKITERWTNSRKRPENVDPGVGKWVS 67
S WE+K R L ++V K + +K W K+ ++ E ++ E +D +W++
Sbjct: 64 SPWEEKSAVAR---LANKVNKSKRNKLWRKKKRKRVVEMLAKEHEQFEQIDREADEWIA 231
>TC226533 similar to GB|AAG53613.1|12407658|AF285832 eukaryotic initiation
factor 3E subunit {Arabidopsis thaliana;} , partial
(98%)
Length = 1619
Score = 30.8 bits (68), Expect = 1.5
Identities = 25/101 (24%), Positives = 41/101 (39%), Gaps = 18/101 (17%)
Frame = +1
Query: 92 LYIYGNVGSGKTMLMDMF------------------YSATEGIVKHRRRYHFHEAMLRIN 133
L+I+ N +G+T ++D+F Y AT IV RRR F + + I
Sbjct: 745 LFIFFNHDNGRTQIIDLFNQDKYLNAIQTSAPHLLRYLATAFIVNKRRRPQFKDFIKVIQ 924
Query: 134 EHMHKTWKKQMEEKPLQSGISSWIMNLPFDTKAKEWLAAEE 174
+ H + P+ ++ +N FD K+ EE
Sbjct: 925 QEQHS------YKDPITEFLACVYVNYDFDGAQKKMRECEE 1029
>TC217238
Length = 1268
Score = 30.0 bits (66), Expect = 2.6
Identities = 14/37 (37%), Positives = 22/37 (58%), Gaps = 1/37 (2%)
Frame = -2
Query: 116 IVKHRRRYHFHEAMLRINEHM-HKTWKKQMEEKPLQS 151
+VKH +HF +A L+ +H+ H Q+E K LQ+
Sbjct: 385 VVKHNLHFHFPQATLKYYKHLEHVNCATQLEVKSLQT 275
>TC228450
Length = 739
Score = 30.0 bits (66), Expect = 2.6
Identities = 16/55 (29%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
Frame = -1
Query: 5 HVNLSNWE---KKRENERRRILMDEVEKQQNDKDWWKRLNNKITERWTNSRKRPE 56
H N+ +WE ++ ENE+ L E++K K W+ + RW + ++R E
Sbjct: 208 HRNVRDWEGLFRRSENEKHLKLKWEMKKAMKMKTNWEWRASASARRWKSRQRRGE 44
>AW830717
Length = 306
Score = 28.9 bits (63), Expect = 5.8
Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
Frame = +1
Query: 132 INEHMHKTWKKQME-EKPLQSGISSWIMN 159
++ H+H+ WK Q E KPL I SW +
Sbjct: 13 VDHHIHRIWKWQSEPNKPLSRLIKSWFFS 99
>TC206116 UP|CC48_SOYBN (P54774) Cell division cycle protein 48 homolog
(Valosin containing protein homolog) (VCP), complete
Length = 2583
Score = 28.9 bits (63), Expect = 5.8
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = +1
Query: 71 REKKLDSLVGRRPTAPPAPKGLYIYGNVGSGKTML 105
R +L +G +P PKG+ +YG GSGKT++
Sbjct: 706 RHPQLFKSIGVKP-----PKGILLYGPPGSGKTLI 795
>TC231751 homologue to GB|BAB08420.1|9757998|AB025622 cell division protein
FtsH protease-like {Arabidopsis thaliana;} , partial
(30%)
Length = 733
Score = 28.5 bits (62), Expect = 7.6
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = +1
Query: 66 VSYLKREKKLDSLVGRRPTAPPAPKGLYIYGNVGSGKTML 105
V YLK K L G+ PKG+ + G G+GKT+L
Sbjct: 139 VEYLKNPAKFTRLGGK------LPKGILLTGPPGTGKTLL 240
>TC225681 homologue to UP|Q9SZD4 (Q9SZD4) 26S proteasome subunit 4-like
protein (26S proteasome subunit AtRPT2a), partial (93%)
Length = 1433
Score = 28.1 bits (61), Expect = 9.9
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +1
Query: 89 PKGLYIYGNVGSGKTMLMDMFYSAT 113
PKG+ +YG G+GKT+L ++T
Sbjct: 571 PKGVILYGEPGTGKTLLAKAVANST 645
>TC225683 homologue to UP|Q9SZD4 (Q9SZD4) 26S proteasome subunit 4-like
protein (26S proteasome subunit AtRPT2a), partial (97%)
Length = 1513
Score = 28.1 bits (61), Expect = 9.9
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +2
Query: 89 PKGLYIYGNVGSGKTMLMDMFYSAT 113
PKG+ +YG G+GKT+L ++T
Sbjct: 674 PKGVILYGEPGTGKTLLAKAVANST 748
>TC213495
Length = 930
Score = 28.1 bits (61), Expect = 9.9
Identities = 15/35 (42%), Positives = 20/35 (56%)
Frame = +2
Query: 276 SEIDYRRFIAQRSENRVNYLWPIERETINKFEKKW 310
S ID F+ E +V LW IERE ++K K+W
Sbjct: 368 SNIDDFNFLKLHQEPQVG-LWFIERERLSKLGKQW 469
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.135 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,890,710
Number of Sequences: 63676
Number of extensions: 307015
Number of successful extensions: 1921
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 1902
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1918
length of query: 520
length of database: 12,639,632
effective HSP length: 102
effective length of query: 418
effective length of database: 6,144,680
effective search space: 2568476240
effective search space used: 2568476240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC145331.1