BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145330.4 + phase: 0
(257 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC216640 similar to GB|AAS56609.1|45270456|AY558283 YHR049W {Sac... 440 e-124
TC207059 230 5e-61
BU964784 173 6e-44
BI968880 168 2e-42
BI787795 73 1e-13
BG155252 51 6e-07
TC219559 similar to UP|Q9SXN4 (Q9SXN4) SLL2-S9-protein, partial ... 28 3.1
CA853145 weakly similar to GP|27901709|gb| gag-pol polyprotein {... 28 3.1
AI495642 weakly similar to GP|10178140|dbj exonuclease-like prot... 27 7.0
TC208602 similar to GB|AAF86996.1|9295690|AC005292 F26F24.7 {Ara... 27 7.0
BQ094881 similar to GP|15982870|gb At1g21880/T26F17_5 {Arabidops... 27 9.2
TC211772 27 9.2
TC225390 homologue to UP|Q84T14 (Q84T14) Vacuolar ATPase subunit... 27 9.2
>TC216640 similar to GB|AAS56609.1|45270456|AY558283 YHR049W {Saccharomyces
cerevisiae;} , partial (9%)
Length = 976
Score = 440 bits (1131), Expect = e-124
Identities = 214/269 (79%), Positives = 237/269 (87%), Gaps = 12/269 (4%)
Frame = +2
Query: 1 MEGENGRKLKILCLHGFRTSGSFIKKQISKWDPSIFSQFHLEFPDGKFPAGGKSDIEGIF 60
MEGENG+KLKILCLHGFRTSGSF+KKQISKWDPS+F QF + FPDGK+PAGGKSDIEGIF
Sbjct: 29 MEGENGKKLKILCLHGFRTSGSFLKKQISKWDPSLFIQFDMVFPDGKYPAGGKSDIEGIF 208
Query: 61 PPPYFEWFQFDKDFTVYTNLDECISYLTEYIIANGPFDGFLGFSQGATLSALLIGYQAQG 120
PPPY+EWFQF+KDFTVY NL+ECISYL +YI ANGPFDGFLGFSQGATLSALLIGYQAQG
Sbjct: 209 PPPYYEWFQFEKDFTVYFNLEECISYLCDYITANGPFDGFLGFSQGATLSALLIGYQAQG 388
Query: 121 KLLKEHPPIKFLVSISGSKFRDPSICDVAYKDPIKAKSVHFIGEKDWLKIPSEELASAFD 180
K+LKEHPPIKF +SISGSKFRDPSICDVAYKDPIKAKSVHFIGEKDWLK+PSE+LASAFD
Sbjct: 389 KVLKEHPPIKFFISISGSKFRDPSICDVAYKDPIKAKSVHFIGEKDWLKLPSEDLASAFD 568
Query: 181 KPLIIRHPQGHTVPRLDEVSTGQLQNFVA-EILSQPKVGVSICEHE-----------SKV 228
KP IIRHPQGHTVPRLDEV+ QL+ ++ E+L QPKV VS+CE E +K+
Sbjct: 569 KPFIIRHPQGHTVPRLDEVAISQLRKWITEEVLCQPKVEVSVCERETDHEEKMSEKTTKI 748
Query: 229 EVDGTNGDKGVNGVEINQGNAETVEVVQA 257
E GTN DKGVNGVEIN+G +ETVEV QA
Sbjct: 749 EPCGTNQDKGVNGVEINKG-SETVEVAQA 832
>TC207059
Length = 1080
Score = 230 bits (586), Expect = 5e-61
Identities = 107/211 (50%), Positives = 139/211 (65%)
Frame = +2
Query: 1 MEGENGRKLKILCLHGFRTSGSFIKKQISKWDPSIFSQFHLEFPDGKFPAGGKSDIEGIF 60
M + RK + LCLHGFRTSG +K Q+ KW S+ L F D FP GKSD+EGIF
Sbjct: 113 MGSDGVRKPRFLCLHGFRTSGEILKTQLHKWPQSVLDNLDLVFVDAPFPCLGKSDVEGIF 292
Query: 61 PPPYFEWFQFDKDFTVYTNLDECISYLTEYIIANGPFDGFLGFSQGATLSALLIGYQAQG 120
PPY+EWFQF+K+FT YTN DEC+ Y+ E +I GP DG LGFSQG+ LSA L G Q +G
Sbjct: 293 DPPYYEWFQFNKEFTEYTNFDECLQYIEECMIKYGPIDGLLGFSQGSILSAALPGLQEKG 472
Query: 121 KLLKEHPPIKFLVSISGSKFRDPSICDVAYKDPIKAKSVHFIGEKDWLKIPSEELASAFD 180
L + P +KFL+ + G+KFR PS+ D AY I S+HFIGE D+L EL +
Sbjct: 473 VALTKVPKVKFLIIVGGAKFRSPSVMDKAYSSSISCPSLHFIGETDFLNKYGAELLESCV 652
Query: 181 KPLIIRHPQGHTVPRLDEVSTGQLQNFVAEI 211
+P++I HP+GHT+PRLD+ S + +F+ I
Sbjct: 653 EPVVIHHPKGHTIPRLDDKSLKTMMDFIERI 745
>BU964784
Length = 456
Score = 173 bits (439), Expect = 6e-44
Identities = 78/137 (56%), Positives = 103/137 (74%)
Frame = +2
Query: 4 ENGRKLKILCLHGFRTSGSFIKKQISKWDPSIFSQFHLEFPDGKFPAGGKSDIEGIFPPP 63
E+ RK++ILCLHGFRTSG +K+ + +W + + L F DG FPA G+SD+EGIF PP
Sbjct: 35 ESQRKVRILCLHGFRTSGEILKQLVLRWPEPVLQKLDLVFLDGPFPAQGRSDVEGIFDPP 214
Query: 64 YFEWFQFDKDFTVYTNLDECISYLTEYIIANGPFDGFLGFSQGATLSALLIGYQAQGKLL 123
YFEWFQ +++FT YTN +EC++Y+ +Y++ NGPFDGFL FSQGA L+A L G QAQG L
Sbjct: 215 YFEWFQANEEFTEYTNFEECLAYIEDYMLKNGPFDGFLSFSQGAILAAALPGMQAQGVAL 394
Query: 124 KEHPPIKFLVSISGSKF 140
+ IKFL+ ISG+KF
Sbjct: 395 GKVDKIKFLIEISGAKF 445
>BI968880
Length = 675
Score = 168 bits (426), Expect = 2e-42
Identities = 89/139 (64%), Positives = 104/139 (74%), Gaps = 12/139 (8%)
Frame = -2
Query: 131 FLVSISGSKFRDPSICDVAYKDPIKAKSVHFIGEKDWLKIPSEELASAFDKPLIIRHPQG 190
F G+ P+ DVAYK IKAKSVHFIGEKDWLK+PSE+L SAFDKPLIIRHPQG
Sbjct: 623 FSYQYQGAXSEIPAYEDVAYKGAIKAKSVHFIGEKDWLKLPSEDLTSAFDKPLIIRHPQG 444
Query: 191 HTVPRLDEVSTGQLQNFVA-EILSQPKVGVSICEHE-----------SKVEVDGTNGDKG 238
HTVPRLDEV+T QL+ ++ E+L QPKVG+S+CEHE +K+E GTN DKG
Sbjct: 443 HTVPRLDEVATSQLRKWITEEVLCQPKVGISVCEHETDHEEKKSEKGTKLEPCGTNQDKG 264
Query: 239 VNGVEINQGNAETVEVVQA 257
VNGVEIN+G +ETVEV QA
Sbjct: 263 VNGVEINKG-SETVEVAQA 210
Score = 42.0 bits (97), Expect = 3e-04
Identities = 21/32 (65%), Positives = 23/32 (71%)
Frame = -1
Query: 114 IGYQAQGKLLKEHPPIKFLVSISGSKFRDPSI 145
IG AQGK L EH IKFL+SISG R+PSI
Sbjct: 675 IGXXAQGKXLXEHXXIKFLISISGCXXRNPSI 580
>BI787795
Length = 420
Score = 73.2 bits (178), Expect = 1e-13
Identities = 41/73 (56%), Positives = 52/73 (71%), Gaps = 12/73 (16%)
Frame = +1
Query: 197 DEVSTGQLQNFVAE-ILSQPKVGVSICEHES-----------KVEVDGTNGDKGVNGVEI 244
DEV+T QL+ ++ E +L QPKVG+S+CEHE+ K+E GTN DKGVNGVEI
Sbjct: 181 DEVATSQLRKWITEEVLCQPKVGISVCEHETDHEEKKSEKGTKLEPCGTNQDKGVNGVEI 360
Query: 245 NQGNAETVEVVQA 257
N+G +ETVEV QA
Sbjct: 361 NKG-SETVEVAQA 396
Score = 70.5 bits (171), Expect = 7e-13
Identities = 36/71 (50%), Positives = 45/71 (62%)
Frame = +2
Query: 165 KDWLKIPSEELASAFDKPLIIRHPQGHTVPRLDEVSTGQLQNFVAEILSQPKVGVSICEH 224
KDWLK+PSE+L SAFDKPLIIRHPQGHTVPRL ++ V Q ++ +S C
Sbjct: 2 KDWLKLPSEDLTSAFDKPLIIRHPQGHTVPRLGMLTLSSPCFLVMFFTLQTELSLSYC*Q 181
Query: 225 ESKVEVDGTNG 235
+ V+ NG
Sbjct: 182 MKWLPVN*ENG 214
>BG155252
Length = 341
Score = 50.8 bits (120), Expect = 6e-07
Identities = 21/39 (53%), Positives = 27/39 (68%)
Frame = -1
Query: 67 WFQFDKDFTVYTNLDECISYLTEYIIANGPFDGFLGFSQ 105
+F ++FT YTN DEC+ Y+ E +I GP DG LGFSQ
Sbjct: 314 FFMVKQEFTEYTNFDECLQYIEECMIKYGPIDGLLGFSQ 198
>TC219559 similar to UP|Q9SXN4 (Q9SXN4) SLL2-S9-protein, partial (86%)
Length = 1124
Score = 28.5 bits (62), Expect = 3.1
Identities = 20/76 (26%), Positives = 34/76 (44%)
Frame = +3
Query: 124 KEHPPIKFLVSISGSKFRDPSICDVAYKDPIKAKSVHFIGEKDWLKIPSEELASAFDKPL 183
K+ P + + S+ G + S D AY D + H + W + E+ +++ K L
Sbjct: 264 KDEPDSEAVTSMLGFQ----SYWDSAYADELTNFREHGHTGEVWFGVDVMEVVASWTKAL 431
Query: 184 IIRHPQGHTVPRLDEV 199
+ QGH +DEV
Sbjct: 432 CVEISQGHIPNGVDEV 479
>CA853145 weakly similar to GP|27901709|gb| gag-pol polyprotein {Vitis
vinifera}, partial (29%)
Length = 563
Score = 28.5 bits (62), Expect = 3.1
Identities = 12/46 (26%), Positives = 24/46 (52%)
Frame = +3
Query: 185 IRHPQGHTVPRLDEVSTGQLQNFVAEILSQPKVGVSICEHESKVEV 230
IR + H GQL+ F+ ++Q K G++IC+ + +++
Sbjct: 39 IRDLKSHLFQHFQTKDLGQLKYFLGIEVAQSKTGIAICQRKYALDI 176
>AI495642 weakly similar to GP|10178140|dbj exonuclease-like protein
{Arabidopsis thaliana}, partial (20%)
Length = 404
Score = 27.3 bits (59), Expect = 7.0
Identities = 19/55 (34%), Positives = 29/55 (52%), Gaps = 3/55 (5%)
Frame = -2
Query: 141 RDPSICDVAYKDPIKAKSVH-FIGEKDWLKIPSE--ELASAFDKPLIIRHPQGHT 192
RD I P++ KS++ F+ KD +PSE ++ SAFD+ I+ Q T
Sbjct: 316 RDYQIVPDKVDSPVERKSLNKFLLAKDQ*DVPSETQKICSAFDRXRILADEQNDT 152
>TC208602 similar to GB|AAF86996.1|9295690|AC005292 F26F24.7 {Arabidopsis
thaliana;} , partial (67%)
Length = 734
Score = 27.3 bits (59), Expect = 7.0
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = -2
Query: 75 TVYTNLDECISYLTEYIIANGPFDGF 100
TVY + +CI+Y+TEY N P F
Sbjct: 700 TVYKSK*KCITYITEYRYLNVPLCNF 623
>BQ094881 similar to GP|15982870|gb At1g21880/T26F17_5 {Arabidopsis
thaliana}, partial (33%)
Length = 421
Score = 26.9 bits (58), Expect = 9.2
Identities = 14/44 (31%), Positives = 24/44 (53%), Gaps = 4/44 (9%)
Frame = -1
Query: 208 VAEILSQPKVGVSICEHESKVEVDGTNGDKGVNGV----EINQG 247
+A + S G++I + EV +NG +G+NG E+N+G
Sbjct: 217 IAIVNSSASHGINIHQISEGGEVPSSNGSEGINGANNIREVNRG 86
>TC211772
Length = 654
Score = 26.9 bits (58), Expect = 9.2
Identities = 12/42 (28%), Positives = 23/42 (54%)
Frame = -1
Query: 121 KLLKEHPPIKFLVSISGSKFRDPSICDVAYKDPIKAKSVHFI 162
K++ ++P + FL+ + + +IC +AYK I H+I
Sbjct: 348 KVIVKYPKLVFLIHHDKTSYHFKNICCLAYKTAI*GDISHYI 223
>TC225390 homologue to UP|Q84T14 (Q84T14) Vacuolar ATPase subunit E
(Fragment), complete
Length = 1143
Score = 26.9 bits (58), Expect = 9.2
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = -1
Query: 17 FRTSGSFIKKQISKWDPSIFSQFHLEFP 44
FR SGSF++K S+ S+FS H FP
Sbjct: 789 FRISGSFLRKTTSRRASSVFS--HTIFP 712
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.140 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,705,232
Number of Sequences: 63676
Number of extensions: 169494
Number of successful extensions: 678
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 671
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 673
length of query: 257
length of database: 12,639,632
effective HSP length: 95
effective length of query: 162
effective length of database: 6,590,412
effective search space: 1067646744
effective search space used: 1067646744
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC145330.4