BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145109.3 - phase: 0
(190 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC215777 similar to UP|OM20_SOLTU (P92792) Mitochondrial import ... 134 2e-32
TC215778 similar to UP|OM20_SOLTU (P92792) Mitochondrial import ... 87 6e-18
TC218992 similar to UP|OM20_SOLTU (P92792) Mitochondrial import ... 77 4e-15
AW459102 61 4e-10
AW707097 similar to EGAD|168613|CPn Lon ATP-dependent protease {... 56 1e-08
AW756028 similar to SP|P92792|OM20_ Mitochondrial import recepto... 54 3e-08
TC226163 similar to UP|Q8S4W7 (Q8S4W7) GAI-like protein 1, parti... 30 0.88
TC235093 similar to UP|Q8MZ21 (Q8MZ21) RE13473p, partial (8%) 28 2.6
TC227399 UP|Q6R0G3 (Q6R0G3) MYB transcription factor, partial (8%) 28 2.6
TC204789 weakly similar to UP|Q6YIA0 (Q6YIA0) Disease resistance... 28 2.6
TC210760 weakly similar to UP|Q9EPR8 (Q9EPR8) Cdk3-binding prote... 28 3.3
TC218782 similar to UP|Q41042 (Q41042) Pisum sativum L. (clone n... 27 4.4
TC218052 similar to UP|2A5D_YEAST (P38903) Serine/threonine prot... 27 5.7
TC218050 weakly similar to UP|Q7PC82 (Q7PC82) PDR14 ABC transpor... 27 5.7
TC229119 27 5.7
AW164266 27 7.4
TC229988 27 7.4
TC227652 similar to UP|Q8LPN7 (Q8LPN7) AT3g19950/MPN9_19, partia... 27 7.4
TC207996 UP|Q6T2Z5 (Q6T2Z5) WD-repeat cell cycle regulatory prot... 27 7.4
>TC215777 similar to UP|OM20_SOLTU (P92792) Mitochondrial import receptor
subunit TOM20 (Translocase of outer membrane 20 kDa
subunit), partial (95%)
Length = 1035
Score = 134 bits (338), Expect = 2e-32
Identities = 83/189 (43%), Positives = 107/189 (55%), Gaps = 13/189 (6%)
Frame = +3
Query: 1 MDYPLRKFKFHLPMKHECILAEQRYNKNPHDAENLTRWGVALLRLSQAHSFPDSLHTIQV 60
MD +F L +H AE Y KNP DA+NLTRWG ALL LSQ SFP+S Q
Sbjct: 165 MDLQQSEFDRLLFFEHARKAAEAEYEKNPLDADNLTRWGGALLELSQFQSFPESKKMTQE 344
Query: 61 SISKLEEAFSLNPNNPDVHWLLGMALTMQALLTPDSHDAKLHFDSADVYFKRAFRQDPSD 120
++SKLEEA ++NP D W LG A T QA L PD +AK++FD A VYF++A +DPS+
Sbjct: 345 AVSKLEEALAVNPKKHDTLWCLGNAHTSQAFLIPDQEEAKVYFDKAAVYFQQAVDEDPSN 524
Query: 121 PTYQISLELPDTKDHEQHPKIVNHGLGQQ--------SKGSSSATKVIK-----HYKFLV 167
Y+ SLE+ K E H +I G GQQ S S+S TK K K+ +
Sbjct: 525 ELYRKSLEVA-AKAPELHVEIHKQGFGQQQQAAATAGSSTSASGTKTQKKKKSSDLKYDI 701
Query: 168 FLFTLLVIG 176
F + +L +G
Sbjct: 702 FGWIILAVG 728
>TC215778 similar to UP|OM20_SOLTU (P92792) Mitochondrial import receptor
subunit TOM20 (Translocase of outer membrane 20 kDa
subunit), partial (72%)
Length = 657
Score = 86.7 bits (213), Expect = 6e-18
Identities = 47/111 (42%), Positives = 65/111 (58%)
Frame = +1
Query: 47 QAHSFPDSLHTIQVSISKLEEAFSLNPNNPDVHWLLGMALTMQALLTPDSHDAKLHFDSA 106
Q SF + Q ++S EEA ++NP D W LG A T QA L PD +AK++FD A
Sbjct: 1 QXXSFXEXKKMTQEAVSXXEEALAVNPKKHDTLWCLGNAHTSQAFLIPDQDEAKVYFDKA 180
Query: 107 DVYFKRAFRQDPSDPTYQISLELPDTKDHEQHPKIVNHGLGQQSKGSSSAT 157
YF++A +DPS+ Y+ SLE+ K E H +I HG GQQ + ++AT
Sbjct: 181 AEYFQQAVDEDPSNELYRKSLEVA-AKAPELHVEIHKHGFGQQQQQQAAAT 330
>TC218992 similar to UP|OM20_SOLTU (P92792) Mitochondrial import receptor
subunit TOM20 (Translocase of outer membrane 20 kDa
subunit), partial (59%)
Length = 561
Score = 77.4 bits (189), Expect = 4e-15
Identities = 47/123 (38%), Positives = 67/123 (54%), Gaps = 8/123 (6%)
Frame = +3
Query: 62 ISKLEEAFSLNPNNPDVHWLLGMALTMQALLTPDSHDAKLHFDSADVYFKRAFRQDPSDP 121
+SKLEE+ +NP D W LG A T A L PD +AK +FD A YF++A +DP +
Sbjct: 3 LSKLEESLLINPTKHDTLWCLGNANTFYAFLIPDITEAKGYFDKALEYFQKAAEEDPEND 182
Query: 122 TYQISLELPDTKDHEQHPKIVNHGLGQQSKGSSSATKVIKH--------YKFLVFLFTLL 173
Y+ SL++ K E H +I +GLG S SSAT K +K+ +F + +L
Sbjct: 183 LYRKSLQVA-VKAPELHMEIHKNGLGLMSNAGSSATSKEKESKKQKSNDFKYDIFGWIIL 359
Query: 174 VIG 176
+G
Sbjct: 360 AVG 368
>AW459102
Length = 428
Score = 60.8 bits (146), Expect = 4e-10
Identities = 33/75 (44%), Positives = 46/75 (61%)
Frame = +3
Query: 83 GMALTMQALLTPDSHDAKLHFDSADVYFKRAFRQDPSDPTYQISLELPDTKDHEQHPKIV 142
G A T QA L PD +AK++FD A YF++A +DPS+ Y+ SLE+ K H +I
Sbjct: 9 GNAHTSQAFLIPDQDEAKVYFDKAAXYFQQAVDEDPSNELYRKSLEVA-AKAPXLHVEIH 185
Query: 143 NHGLGQQSKGSSSAT 157
HG GQQ + ++AT
Sbjct: 186 KHGFGQQQQQXAAAT 230
>AW707097 similar to EGAD|168613|CPn Lon ATP-dependent protease {Chlamydia
pneumoniae}, partial (2%)
Length = 379
Score = 55.8 bits (133), Expect = 1e-08
Identities = 39/126 (30%), Positives = 56/126 (43%)
Frame = +2
Query: 2 DYPLRKFKFHLPMKHECILAEQRYNKNPHDAENLTRWGVALLRLSQAHSFPDSLHTIQVS 61
D KF L H E +Y KNP A NLT+ + +R+ F S
Sbjct: 2 DLQQSKFNHLLFFDHTHKATETKY*KNPLYANNLTK*K*SFIRIISISEFS*IEENNPKS 181
Query: 62 ISKLEEAFSLNPNNPDVHWLLGMALTMQALLTPDSHDAKLHFDSADVYFKRAFRQDPSDP 121
K ++NP + L T QA L PD++ K++FD A YF++A ++PS+
Sbjct: 182 *FKARRTLTINPKXHNTL*YLENTHTSQAFLIPDANKTKVYFDKAAKYFQQAINKNPSNK 361
Query: 122 TYQISL 127
Y SL
Sbjct: 362 LYHKSL 379
>AW756028 similar to SP|P92792|OM20_ Mitochondrial import receptor subunit
TOM20 (Translocase of outer membrane 20 kDa subunit).
[Potato], partial (25%)
Length = 450
Score = 54.3 bits (129), Expect = 3e-08
Identities = 29/54 (53%), Positives = 33/54 (60%)
Frame = +1
Query: 1 MDYPLRKFKFHLPMKHECILAEQRYNKNPHDAENLTRWGVALLRLSQAHSFPDS 54
MD +F L +H AE Y KNP DA+NLTRWG ALL LSQ SFP+S
Sbjct: 79 MDLQQSEFDRLLFFEHARKAAEAEYEKNPLDADNLTRWGGALLELSQFQSFPES 240
>TC226163 similar to UP|Q8S4W7 (Q8S4W7) GAI-like protein 1, partial (20%)
Length = 859
Score = 29.6 bits (65), Expect = 0.88
Identities = 16/34 (47%), Positives = 21/34 (61%)
Frame = +1
Query: 42 LLRLSQAHSFPDSLHTIQVSISKLEEAFSLNPNN 75
L RLS +H FP+SLH +I L E +NPN+
Sbjct: 43 LYRLSLSHGFPESLHYYS-TILDLLEGSPVNPND 141
>TC235093 similar to UP|Q8MZ21 (Q8MZ21) RE13473p, partial (8%)
Length = 967
Score = 28.1 bits (61), Expect = 2.6
Identities = 19/79 (24%), Positives = 36/79 (45%)
Frame = -1
Query: 11 HLPMKHECILAEQRYNKNPHDAENLTRWGVALLRLSQAHSFPDSLHTIQVSISKLEEAFS 70
H P+KH I R++ H +N+ + L +S + D + I VSI + +
Sbjct: 676 HQPLKHTSISLHIRFHSLIHHLQNVLTSFLHKLNISSSSKTLDK-NRIGVSIGRDPRSQH 500
Query: 71 LNPNNPDVHWLLGMALTMQ 89
+ N + WL G+ + ++
Sbjct: 499 AHVNLESLFWLSGVGVGLE 443
>TC227399 UP|Q6R0G3 (Q6R0G3) MYB transcription factor, partial (8%)
Length = 1088
Score = 28.1 bits (61), Expect = 2.6
Identities = 15/39 (38%), Positives = 18/39 (45%)
Frame = +1
Query: 118 PSDPTYQISLELPDTKDHEQHPKIVNHGLGQQSKGSSSA 156
P P SL+ D Q PK V G+G S GSS +
Sbjct: 121 PEQPMKSNSLKSSDFDQENQSPKSVLSGVGSDSLGSSDS 237
>TC204789 weakly similar to UP|Q6YIA0 (Q6YIA0) Disease resistance
protein-like protein MsR1, partial (76%)
Length = 2649
Score = 28.1 bits (61), Expect = 2.6
Identities = 16/47 (34%), Positives = 20/47 (42%)
Frame = +2
Query: 38 WGVALLRLSQAHSFPDSLHTIQVSISKLEEAFSLNPNNPDVHWLLGM 84
W + LSQ HS DS + KL NPNN + LG+
Sbjct: 896 WQKMVEELSQGHSILDSNIELLTCFQKLLHVLEDNPNNKECFMDLGL 1036
>TC210760 weakly similar to UP|Q9EPR8 (Q9EPR8) Cdk3-binding protein ik3-1,
partial (4%)
Length = 826
Score = 27.7 bits (60), Expect = 3.3
Identities = 30/118 (25%), Positives = 46/118 (38%), Gaps = 1/118 (0%)
Frame = +2
Query: 53 DSLHTIQVSISKLEEAFSLNPNNPDVHWLLGMALTMQALLTPDSHDAKLH-FDSADVYFK 111
++LH +Q S L L+P+N H+ LTP + ++H FD A
Sbjct: 194 NALHKLQNSHPILRSKIHLDPSNNTFHF-----------LTPPTPTVQIHPFDLASTAHI 340
Query: 112 RAFRQDPSDPTYQISLELPDTKDHEQHPKIVNHGLGQQSKGSSSATKVIKHYKFLVFL 169
+ D DP + + LE HE + + +A I H +F VFL
Sbjct: 341 IQCQSDGHDP-FHVLLE------HEMNQEDTWRNYAADVDVLHAAVYSISHDRFAVFL 493
>TC218782 similar to UP|Q41042 (Q41042) Pisum sativum L. (clone na-481-5),
partial (20%)
Length = 925
Score = 27.3 bits (59), Expect = 4.4
Identities = 15/43 (34%), Positives = 21/43 (47%), Gaps = 2/43 (4%)
Frame = -2
Query: 11 HLPMKHECILAEQRYNKNPHDAEN--LTRWGVALLRLSQAHSF 51
HLP H C+ +E+ + PH L + LL SQ+ SF
Sbjct: 864 HLPSPHPCLFSEEFWRVPPHFHPRCLLRNYHCFLLDFSQSFSF 736
>TC218052 similar to UP|2A5D_YEAST (P38903) Serine/threonine protein
phosphatase 2A, 56 kDa regulatory subunit, delta isoform
(PP2A, B subunit, B' delta isoform) (RTS1 protein) (SCS1
protein), partial (3%)
Length = 1248
Score = 26.9 bits (58), Expect = 5.7
Identities = 18/57 (31%), Positives = 31/57 (53%), Gaps = 3/57 (5%)
Frame = -2
Query: 121 PTYQISLEL---PDTKDHEQHPKIVNHGLGQQSKGSSSATKVIKHYKFLVFLFTLLV 174
P++ +S + P T HE HP I+ + SK + + T ++ +KFL+ L LL+
Sbjct: 569 PSHSLSKHVSVTPSTVTHEHHPPIIVR--LRFSKLTHTGTFLLIIFKFLLLLLLLLL 405
>TC218050 weakly similar to UP|Q7PC82 (Q7PC82) PDR14 ABC transporter, partial
(28%)
Length = 1436
Score = 26.9 bits (58), Expect = 5.7
Identities = 24/86 (27%), Positives = 36/86 (40%)
Frame = +2
Query: 63 SKLEEAFSLNPNNPDVHWLLGMALTMQALLTPDSHDAKLHFDSADVYFKRAFRQDPSDPT 122
S+L E F P P + A T T S +A+L D A +Y + +D +
Sbjct: 242 SRLIEYFQNIPGVPKIKDNYNPA-TWMLEATSASVEAELKIDFAQIYKESHLCRDTLELV 418
Query: 123 YQISLELPDTKDHEQHPKIVNHGLGQ 148
++S P TKD + + LGQ
Sbjct: 419 RELSEPPPGTKDLHFSTRFPQNSLGQ 496
>TC229119
Length = 455
Score = 26.9 bits (58), Expect = 5.7
Identities = 18/64 (28%), Positives = 33/64 (51%), Gaps = 14/64 (21%)
Frame = -1
Query: 96 SHDAKLHFDSADVYFKRAFRQDP------------SDPTYQISLELPDTKDHEQH--PKI 141
++ AKL +DS+ V R + +P ++PT Q SL LP + ++ + KI
Sbjct: 344 TNSAKLEYDSSQVSVDRQIQHNPCFAMHRE*GTEVTNPTNQGSLHLPFNQKYDDNWPSKI 165
Query: 142 VNHG 145
+++G
Sbjct: 164 LHYG 153
>AW164266
Length = 435
Score = 26.6 bits (57), Expect = 7.4
Identities = 18/66 (27%), Positives = 27/66 (40%)
Frame = +3
Query: 2 DYPLRKFKFHLPMKHECILAEQRYNKNPHDAENLTRWGVALLRLSQAHSFPDSLHTIQVS 61
D+ + LP I + R N + ENLT W S+ F ++ +
Sbjct: 27 DFTEELLQIELPESTSLIEEKFRSNMDEILEENLTFWLKFSAYYSEIQKFETTIKDLLTE 206
Query: 62 ISKLEE 67
+SKLEE
Sbjct: 207LSKLEE 224
>TC229988
Length = 316
Score = 26.6 bits (57), Expect = 7.4
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = -1
Query: 12 LPMKHECILAEQRYNKNPHD 31
L +KHEC L E + K P D
Sbjct: 271 LKLKHECFLCEMKKKKGPMD 212
>TC227652 similar to UP|Q8LPN7 (Q8LPN7) AT3g19950/MPN9_19, partial (35%)
Length = 1209
Score = 26.6 bits (57), Expect = 7.4
Identities = 9/35 (25%), Positives = 15/35 (42%)
Frame = -1
Query: 6 RKFKFHLPMKHECILAEQRYNKNPHDAENLTRWGV 40
R+F H H+ R+++ PH RW +
Sbjct: 825 RRFHHHRRRSHQSSTRRHRHSREPHSRNPRRRWAI 721
>TC207996 UP|Q6T2Z5 (Q6T2Z5) WD-repeat cell cycle regulatory protein,
complete
Length = 2059
Score = 26.6 bits (57), Expect = 7.4
Identities = 16/53 (30%), Positives = 25/53 (46%)
Frame = +2
Query: 120 DPTYQISLELPDTKDHEQHPKIVNHGLGQQSKGSSSATKVIKHYKFLVFLFTL 172
+PT Q+ L T+ + P S SSS++ + + + FLV LF L
Sbjct: 5 EPTQQLLSLLSHTQKERERPS-------SSSSSSSSSSSIAQEHIFLVHLFLL 142
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.136 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,647,996
Number of Sequences: 63676
Number of extensions: 131750
Number of successful extensions: 684
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 680
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 680
length of query: 190
length of database: 12,639,632
effective HSP length: 92
effective length of query: 98
effective length of database: 6,781,440
effective search space: 664581120
effective search space used: 664581120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC145109.3