BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145061.27 - phase: 0 /pseudo
(615 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NP005379 ORF (334 AA) 251 7e-67
CF922321 182 3e-46
TC233500 similar to UP|Q6I8M7 (Q6I8M7) Soybean Tgm5 transposable... 151 9e-37
CF922798 134 9e-32
TC233466 similar to UP|Q6I8M7 (Q6I8M7) Soybean Tgm5 transposable... 72 6e-13
TC212179 49 9e-06
BE440417 42 0.001
BI425208 39 0.009
TC234090 31 1.4
TC207572 similar to UP|Q8AVP1 (Q8AVP1) Rpf1-pending-prov protein... 31 1.4
BE806169 31 1.8
AW200904 31 1.8
TC226889 homologue to UP|Q9SXP4 (Q9SXP4) DNA-binding protein NtW... 30 3.2
TC229259 weakly similar to UP|YPL6_ARATH (Q9FN32) Yippee-like pr... 30 4.1
TC224833 homologue to UP|UBCA_ARATH (P35133) Ubiquitin-conjugati... 30 4.1
TC224915 homologue to UP|UBCA_ARATH (P35133) Ubiquitin-conjugati... 29 5.4
TC213207 weakly similar to GB|AAQ65132.1|34365641|BT010509 At3g4... 29 5.4
TC210762 28 9.2
AW705803 PIR|B86141|B86 protein T25K16.3 [imported] - Arabidopsi... 28 9.2
>NP005379 ORF (334 AA)
Length = 1002
Score = 251 bits (641), Expect = 7e-67
Identities = 140/329 (42%), Positives = 193/329 (58%), Gaps = 5/329 (1%)
Frame = +1
Query: 195 IMRDTTPVKDNLPLSFYEAQKLVEKLGLEVKTTDCCVNGCMLFYDNEFGKNDGALVACKF 254
I+ D P + LP S+Y+A+K++ +G+E + C N C+L Y +EF + + C
Sbjct: 40 IVHDLLPQDNTLPKSYYQAKKILCPMGMEYQKIHACPNDCIL-YRHEFEE----MSKCPR 204
Query: 255 CNAPRYEVCDD----AGSQKKKRVSIKSMFYLPIIPRLQRLFASTHTADKMTWHYYNKPN 310
C A RY+V DD + KK K ++YLPIIPR +RLFA+ A +TWH + +
Sbjct: 205 CGASRYKVKDDEDCSSDENSKKGPPAKVLWYLPIIPRFKRLFANEDDAKDLTWHANGRKS 384
Query: 311 SGVMRHPCDGVAWKHFDQVHRDFAEDPHNVRLGLCSDGFIPYIQASATPYSCWPILLTPY 370
G++RHP D WK D ++ +F ++ N+RLGL SDG PY S T +S WP+LL Y
Sbjct: 385 DGMVRHPADCSQWKKIDSLYPNFGKEARNLRLGLASDGMNPYGNLS-TQHSSWPVLLVIY 561
Query: 371 NVPLEMCMSKPYLFLSCIVPGPTSSLDGIDVYIQPLIDDLNRLWN-GVSTYDIARKKIFR 429
N P +CM + Y+ LS ++ GP + IDVY+ PLI+DL +LW+ GV +D RK+ F+
Sbjct: 562 NFPPWLCMKRKYMMLSMMISGPRQPGNDIDVYLSPLIEDLRKLWDEGVLVFDGFRKETFQ 741
Query: 430 MRAALMWTINDFPAYGMLS*WSTHGRLACPHCMEQTKAIWFHHGRKHS*FDCHRPFLPAT 489
MRA L TINDFPAYG LS +S G LACP C E T I HGRK + + HR FL A
Sbjct: 742 MRAMLFCTINDFPAYGNLSGYSVKGHLACPICEEDTSYIQLKHGRK-TVYTRHRVFLKAH 918
Query: 490 HEFRKKKNLFTKGKTEKDGPPPRTTSEEV 518
H +R+ K F G E + T E+V
Sbjct: 919 HPYRRLKKAF-NGSQEHEIRRTPLTGEQV 1002
>CF922321
Length = 742
Score = 182 bits (463), Expect = 3e-46
Identities = 102/243 (41%), Positives = 141/243 (57%), Gaps = 1/243 (0%)
Frame = -1
Query: 284 IIPRLQRLFASTHTADKMTWHYYNKPNSGVMRHPCDGVAWKHFDQVHRDFAEDPHNVRLG 343
IIPRL+RLFA+ A +TWH + G++R WK D+++ D ++ N+RLG
Sbjct: 742 IIPRLKRLFANGDDAKDLTWHANGRNCDGMIRRLAVSSQWKKIDRLYLDLGKEASNLRLG 563
Query: 344 LCSDGFIPYIQASATPYSCWPILLTPYNVPLEMCMSKPYLFLSCIVPGPTSSLDGIDVYI 403
L SDG PY +S+T +S WP+LL YN+P +CM + Y+ LS ++ P + IDVY+
Sbjct: 562 LASDGMNPY-GSSST*HSSWPVLLVIYNLPPWLCMKRKYMMLSMMISNPRQPGNDIDVYL 386
Query: 404 QPLIDDLNRLWN-GVSTYDIARKKIFRMRAALMWTINDFPAYGMLS*WSTHGRLACPHCM 462
LI+DL +LW+ G ++ + F MRA L TINDFPAYG LS ++ G +CP C
Sbjct: 385 SSLIEDLTKLWDEGFLAFNGFWNETFPMRAMLFCTINDFPAYGNLSGYNVKGHHSCPICE 206
Query: 463 EQTKAIWFHHGRKHS*FDCHRPFLPATHEFRKKKNLFTKGKTEKDGPPPRTTSEEVYRRV 522
E T I HGRK + + HR FL H +R+ K F G D P T+EEVY+RV
Sbjct: 205 ENTSYIQLKHGRK-TVYSRHRRFLTPNHPYRRLKKAF-NGSQGHDIVPIPLTAEEVYQRV 32
Query: 523 NEL 525
L
Sbjct: 31 QHL 23
>TC233500 similar to UP|Q6I8M7 (Q6I8M7) Soybean Tgm5 transposable element
(Fragment), partial (65%)
Length = 1033
Score = 151 bits (381), Expect = 9e-37
Identities = 83/222 (37%), Positives = 125/222 (55%), Gaps = 1/222 (0%)
Frame = +2
Query: 301 MTWHYYNKPNSGVMRHPCDGVAWKHFDQVHRDFAEDPHNVRLGLCSDGFIPYIQASATPY 360
+TWH + ++RH D + WK D+++ DF ++ N+ LGL +D PY +T +
Sbjct: 8 LTWHANGRNCDRMLRHLADSLQWKKIDRLYPDFGKETKNLTLGLATDAMNPY-GCLSTQH 184
Query: 361 SCWPILLTPYNVPLEMCMSKPYLFLSCIVPGPTSSLDGIDVYIQPLIDDLNRLWN-GVST 419
+ WP+LL YN+P +CM + Y+ LS ++ GP + I+VY+ PLI+DL +L + GV
Sbjct: 185 NSWPVLLVIYNLPHWLCMKRKYMMLSMMITGPRQPGNDINVYLNPLIEDLTKL*DEGVLV 364
Query: 420 YDIARKKIFRMRAALMWTINDFPAYGMLS*WSTHGRLACPHCMEQTKAIWFHHGRKHS*F 479
+D R + F + A L TINDFP Y LS +S G ACP C E T I HGRK + +
Sbjct: 365 FDGFRNETFNLCAMLFCTINDFPTYENLSGYSVKGHRACPICEEDTSYIQQKHGRK-TVY 541
Query: 480 DCHRPFLPATHEFRKKKNLFTKGKTEKDGPPPRTTSEEVYRR 521
HR FL H +R+ K F + E + T ++V++R
Sbjct: 542 TRHRRFLKPHHPYRRLKKAFNE-S*EHENALVLLTGDQVFQR 664
>CF922798
Length = 617
Score = 134 bits (338), Expect = 9e-32
Identities = 80/203 (39%), Positives = 113/203 (55%), Gaps = 1/203 (0%)
Frame = -2
Query: 327 DQVHRDFAEDPHNVRLGLCSDGFIPYIQASATPYSCWPILLTPYNVPLEMCMSKPYLFLS 386
D ++ DF+++ N+RLGL +DG PY S T YS W +LL YN+ +CM+K Y+ LS
Sbjct: 613 DHLYLDFSKEAINLRLGLATDGMNPYGSLS-TQYSLWSVLLVIYNLSPWLCMNKKYMMLS 437
Query: 387 CIVPGPTSSLDGIDVYIQPLIDDLNRLWN-GVSTYDIARKKIFRMRAALMWTINDFPAYG 445
++ P + IDVY+ PLI+DL L + V +D + + F + A L TINDFPAYG
Sbjct: 436 MMILSPRQPGNDIDVYLSPLIEDLTML*DEEVLVFDGFQNETFHLCAMLFCTINDFPAYG 257
Query: 446 MLS*WSTHGRLACPHCMEQTKAIWFHHGRKHS*FDCHRPFLPATHEFRKKKNLFTKGKTE 505
LS +S ACP C E T I HGRK + + H+ FL H +R+ K F G E
Sbjct: 256 NLSGYSVKSHRACPICEEDTSYIQLKHGRK-TIYTRHQRFLKPHHLYRRLKKEF-NGSQE 83
Query: 506 KDGPPPRTTSEEVYRRVNELGLL 528
+ P + VY++V L +
Sbjct: 82 HETTPILLVGDHVYQQVQHLNTI 14
>TC233466 similar to UP|Q6I8M7 (Q6I8M7) Soybean Tgm5 transposable element
(Fragment), partial (37%)
Length = 790
Score = 72.4 bits (176), Expect = 6e-13
Identities = 39/114 (34%), Positives = 63/114 (55%), Gaps = 4/114 (3%)
Frame = +2
Query: 195 IMRDTTPVKDNLPLSFYEAQKLVEKLGLEVKTTDCCVNGCMLFYDNEFGKNDGALVACKF 254
++ D P ++ LP S+Y+A+K++ + +E C N C+L Y +EF + + C
Sbjct: 455 VVHDVLPEENTLPKSYYQAKKILCSMDMEY*KIHACPNDCIL-YRHEFEE----MSKCPR 619
Query: 255 CNAPRYEVCDD----AGSQKKKRVSIKSMFYLPIIPRLQRLFASTHTADKMTWH 304
C RY+V DD + KK K ++YLPI+P+ +RLFA+ A +TWH
Sbjct: 620 CGVSRYKVKDDEECSSDENSKKGPLAKVLWYLPIVPKFKRLFANGDDAKDLTWH 781
Score = 40.0 bits (92), Expect = 0.003
Identities = 23/78 (29%), Positives = 32/78 (40%), Gaps = 4/78 (5%)
Frame = +3
Query: 28 DGVRDFVAYAMAQDAFQLEGGIRCSCVKCTCRLIRSPKDVLNHLKDLGFMENYYVWIYHG 87
+GV F+ A + G C C+ C + D+ +HL NY WI+HG
Sbjct: 9 EGVEQFLQLASERSRPDENGKFFCPCINCLNGRRQKVNDIRDHLLCDEIKRNYTTWIWHG 188
Query: 88 E----QEPTNNTEFDVNM 101
E Q + FDV M
Sbjct: 189 EMADMQSGPQSEPFDVEM 242
>TC212179
Length = 764
Score = 48.5 bits (114), Expect = 9e-06
Identities = 26/93 (27%), Positives = 41/93 (43%), Gaps = 4/93 (4%)
Frame = +1
Query: 13 DRKYPGKRKLKAAFVDGVRDFVAYAMAQDAFQLEGGIRCSCVKCTCRLIRSPKDVLNHLK 72
DR + + ++ + +GV F+ +A + +G C C+ C + D+ HL
Sbjct: 34 DRSWMNQSRISPEYEEGVEQFLQFASQRGQPDEDGKYYCPCINCLNGRRQILDDIREHLL 213
Query: 73 DLGFMENYYVWIYHGE----QEPTNNTEFDVNM 101
G NY WI+HGE Q + FDV M
Sbjct: 214 CDGIKRNYTTWIWHGEMTDMQSGQQSEPFDVEM 312
>BE440417
Length = 192
Score = 41.6 bits (96), Expect = 0.001
Identities = 23/55 (41%), Positives = 28/55 (50%)
Frame = +2
Query: 464 QTKAIWFHHGRKHS*FDCHRPFLPATHEFRKKKNLFTKGKTEKDGPPPRTTSEEV 518
Q K + FH K FD H FLP H +R+ K F KG E PPP +S +V
Sbjct: 11 QFKTLNFH---KICYFDFHHQFLPLNHAYRRNKKSFKKGWVETSPPPPCLSSLDV 166
>BI425208
Length = 413
Score = 38.5 bits (88), Expect = 0.009
Identities = 17/63 (26%), Positives = 32/63 (49%)
Frame = -3
Query: 26 FVDGVRDFVAYAMAQDAFQLEGGIRCSCVKCTCRLIRSPKDVLNHLKDLGFMENYYVWIY 85
++ G+ DF+ ++ + + + IRC C +C ++ +V+ L F +NY W
Sbjct: 186 YLKGINDFLEFSFSYS--KNKNTIRCPCCRCNAIYYKTRDEVIGDLLKSRFQQNYEHWES 13
Query: 86 HGE 88
HGE
Sbjct: 12 HGE 4
>TC234090
Length = 420
Score = 31.2 bits (69), Expect = 1.4
Identities = 30/103 (29%), Positives = 49/103 (47%), Gaps = 5/103 (4%)
Frame = -1
Query: 479 FDCHRPFLPATHEFRKKKNLFTKGKTEKDGPPPRTTSEEVYRRVNELGLLKFTDV----G 534
F CH FLP + +KKN + + P + EE++ R ++L + V G
Sbjct: 390 FYCHYHFLPR*CSWERKKNKKLRDIVLRSLSP--LSGEEIWPRFSQLSFIYGNFVRSYLG 217
Query: 535 KRVRHPKYGNEHHWTKRSIFWDLPYWK-DNLLRHNFDVMHIEK 576
R+ G +RS+F +L + + NLL N +VMHI++
Sbjct: 216 IRMVETIIG------QRSVF*NLLH*RMHNLLHDNINVMHIKR 106
>TC207572 similar to UP|Q8AVP1 (Q8AVP1) Rpf1-pending-prov protein, partial
(37%)
Length = 1183
Score = 31.2 bits (69), Expect = 1.4
Identities = 24/89 (26%), Positives = 42/89 (46%), Gaps = 3/89 (3%)
Frame = +3
Query: 466 KAIWFHHGRKHS*FDCHRPFLPATHEFRKKKNLFTKGKTEKDGPPPRTTSEEVYRRVNEL 525
+ + FH+ R F HR ++ T E +K ++ KGK +KDG ++ R+ E
Sbjct: 639 RVVTFHNQRDFIFFRHHR-YIFETKEVKKAES---KGKKDKDGESENVPEQKTIARLQEC 806
Query: 526 GLLKFTDVGKRVRHPKY---GNEHHWTKR 551
G +FT ++H + G E+ W +
Sbjct: 807 G-PRFTLKLISLQHGTFDTKGGEYEWVHK 890
>BE806169
Length = 396
Score = 30.8 bits (68), Expect = 1.8
Identities = 20/58 (34%), Positives = 30/58 (51%)
Frame = +1
Query: 138 EETIPNEKALKFYKMMQEVNKPLFEGSSDSKLSMSVRLLAAASDWSVAEEGSECYTDI 195
+E + +K + + + ++V K L GS S LLA S +S A +GSE Y DI
Sbjct: 43 DEQLKQKKKISYVRRRKDVFKTLGPGSEVESAS----LLAFGSLFSAAYKGSELYVDI 204
>AW200904
Length = 174
Score = 30.8 bits (68), Expect = 1.8
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = -1
Query: 438 INDFPAYGMLS*WSTHGRLACPHCMEQTKAIWFHHGRKHS 477
+N FPA+ +ST R HC E++K IWF H+
Sbjct: 162 LNSFPAH-----FSTLPRRCADHCCERSKTIWFTXQEIHA 58
>TC226889 homologue to UP|Q9SXP4 (Q9SXP4) DNA-binding protein NtWRKY3,
partial (11%)
Length = 471
Score = 30.0 bits (66), Expect = 3.2
Identities = 15/48 (31%), Positives = 30/48 (62%), Gaps = 1/48 (2%)
Frame = +1
Query: 548 WTKRSIFW-DLPYWKDNLLRHNFDVMHIEKNFCDNIIHIVMNVSAKTK 594
WTKR ++W D ++ + L N+++++++ FC +I I ++S K K
Sbjct: 313 WTKRVVYWMDKDFYVN--LTKNYEILYLDMFFCSYLIDI-HSLSTKQK 447
>TC229259 weakly similar to UP|YPL6_ARATH (Q9FN32) Yippee-like protein
At5g53940, partial (83%)
Length = 882
Score = 29.6 bits (65), Expect = 4.1
Identities = 16/37 (43%), Positives = 18/37 (48%), Gaps = 7/37 (18%)
Frame = +3
Query: 229 CCVNGCMLFYDNEFGKN-------DGALVACKFCNAP 258
CCV C FY++EF DG CKFCN P
Sbjct: 105 CCVVLCG-FYESEFHMGRVFVLELDGRSYGCKFCNTP 212
>TC224833 homologue to UP|UBCA_ARATH (P35133) Ubiquitin-conjugating enzyme
E2-17 kDa 10/12 (Ubiquitin-protein ligase 10/12)
(Ubiquitin carrier protein 10/12) , complete
Length = 683
Score = 29.6 bits (65), Expect = 4.1
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -3
Query: 51 CSCVKCTCRLIRSPKD 66
C C+ CTC L R PKD
Sbjct: 630 CFCLSCTCGLFRGPKD 583
>TC224915 homologue to UP|UBCA_ARATH (P35133) Ubiquitin-conjugating enzyme
E2-17 kDa 10/12 (Ubiquitin-protein ligase 10/12)
(Ubiquitin carrier protein 10/12) , complete
Length = 957
Score = 29.3 bits (64), Expect = 5.4
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -1
Query: 51 CSCVKCTCRLIRSPKD 66
CSC+ CTC R PKD
Sbjct: 555 CSCLSCTCVQFRGPKD 508
>TC213207 weakly similar to GB|AAQ65132.1|34365641|BT010509 At3g48180
{Arabidopsis thaliana;} , partial (58%)
Length = 429
Score = 29.3 bits (64), Expect = 5.4
Identities = 16/57 (28%), Positives = 28/57 (49%), Gaps = 3/57 (5%)
Frame = -3
Query: 266 AGSQKKKRVSIKSMFYLPIIPRLQ---RLFASTHTADKMTWHYYNKPNSGVMRHPCD 319
+G Q++ S S + P++ Q R FA + + Y+N+P+ G+ HP D
Sbjct: 235 SGGQRRYHPSTSSHRWRPLLWSCQNPHRAFACIWRCCRCCFCYWNQPSQGLAHHPTD 65
>TC210762
Length = 857
Score = 28.5 bits (62), Expect = 9.2
Identities = 13/31 (41%), Positives = 20/31 (63%)
Frame = +3
Query: 382 YLFLSCIVPGPTSSLDGIDVYIQPLIDDLNR 412
YL L+ +VP TS + + + +QP +D LNR
Sbjct: 108 YLLLNALVPLLTSLVALVPILLQPPLDSLNR 200
>AW705803 PIR|B86141|B86 protein T25K16.3 [imported] - Arabidopsis thaliana,
partial (4%)
Length = 416
Score = 28.5 bits (62), Expect = 9.2
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +1
Query: 460 HCMEQTKAIWFHHG 473
HC E+TKAIW HG
Sbjct: 202 HCREKTKAIWGQHG 243
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.324 0.138 0.450
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,577,369
Number of Sequences: 63676
Number of extensions: 556033
Number of successful extensions: 2476
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 2449
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2462
length of query: 615
length of database: 12,639,632
effective HSP length: 103
effective length of query: 512
effective length of database: 6,081,004
effective search space: 3113474048
effective search space used: 3113474048
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 62 (28.5 bits)
Medicago: description of AC145061.27