BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144893.1 + phase: 0 /pseudo
(311 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC232778 similar to UP|Q9LNG5 (Q9LNG5) F21D18.16, partial (5%) 86 3e-17
BQ576456 80 2e-15
TC211858 52 1e-12
BI470376 weakly similar to GP|14090338|dbj P0638D12.5 {Oryza sat... 52 3e-07
BE475635 47 1e-05
CO979307 40 0.001
TC211545 weakly similar to UP|Q7XHQ1 (Q7XHQ1) Calcineurin-like p... 36 0.020
AI495080 33 0.13
TC208196 32 0.48
TC208195 30 1.4
BE805433 29 2.4
AI959929 29 3.1
BG839790 similar to PIR|D96521|D96 protein F21D18.16 [imported] ... 28 5.3
AI899862 28 5.3
TC204009 homologue to UP|Q9XQB6 (Q9XQB6) Chlorophyll a/b-binding... 28 5.3
AI973900 27 9.1
TC218035 27 9.1
TC211576 weakly similar to UP|Q7Q720 (Q7Q720) EbiP7196 (Fragment... 27 9.1
>TC232778 similar to UP|Q9LNG5 (Q9LNG5) F21D18.16, partial (5%)
Length = 746
Score = 85.5 bits (210), Expect = 3e-17
Identities = 61/182 (33%), Positives = 93/182 (50%)
Frame = -1
Query: 26 WLQDTSYIMIDHGLIYAFVEMWHEETSSFHLPFEEITVTLDDVTYLLHIPIDDMLLSHGF 85
W+ Y+ I+ LI AF+E W ET +FHL E T+TL DV LL + D L G
Sbjct: 521 WIMKMGYLKINAALISAFIERWRPETHTFHLRCGEATITLQDVLILLGLRTDGAPLI-GS 345
Query: 86 ISRDEAVELMEEYLESDTGDALIEVDKTKGAHCRFSYLEKIFKECLKEQRDLAAEYGVTE 145
+ D A +L EE L + IE G+ + S+L F + + G E
Sbjct: 344 TNLDWA-DLCEELLGVRPQEGEIE-----GSVVKLSWLAHYFSH-------INIDEGNVE 204
Query: 146 EVERLQDQTVRIYLLYLVGITIFTDKSQWAVDVVYLKFFRNIDLVSSFSWGVAALAHLY* 205
+++R +R ++L +G IF +KS V + YL+F R+ + S+++WG A LA+LY
Sbjct: 203 QLQRF----IRAWILRFIGGVIFVNKSSSRVSLRYLQFLRDFE*CSTYAWGPAMLAYLYR 36
Query: 206 EL 207
E+
Sbjct: 35 EM 30
>BQ576456
Length = 424
Score = 79.7 bits (195), Expect = 2e-15
Identities = 50/145 (34%), Positives = 77/145 (52%)
Frame = +1
Query: 60 EITVTLDDVTYLLHIPIDDMLLSHGFISRDEAVELMEEYLESDTGDALIEVDKTKGAHCR 119
E+T+TLDD+ LLH+PI L + DEAV L+ E LE +A ++ + A+ R
Sbjct: 34 ELTITLDDMACLLHLPITGALHRFEPLGVDEAVLLLMELLEVSREEARAKIVRAHRAYVR 213
Query: 120 FSYLEKIFKECLKEQRDLAAEYGVTEEVERLQDQTVRIYLLYLVGITIFTDKSQWAVDVV 179
S+L ++++ + + + A VR YL +LV T+F +KS V VV
Sbjct: 214 LSWLREVYQSRCQARCWIVA---------------VRAYLFHLVDCTLFANKSATHVHVV 348
Query: 180 YLKFFRNIDLVSSFSWGVAALAHLY 204
+LK F ++ + WGVAAL H+Y
Sbjct: 349 HLKGF*DLC*SGGYGWGVAALVHMY 423
>TC211858
Length = 524
Score = 52.0 bits (123), Expect(3) = 1e-12
Identities = 24/49 (48%), Positives = 33/49 (66%)
Frame = -1
Query: 176 VDVVYLKFFRNIDLVSSFSWGVAALAHLY*ELNNAARWNCSQMAGYLTL 224
V VVYL FR++ ++WGVAAL H+Y +L+ A+R Q+ GYLTL
Sbjct: 470 VHVVYLDAFRDLGQSGGYAWGVAALVHMYNQLDEASRTTTRQIVGYLTL 324
Score = 32.3 bits (72), Expect(3) = 1e-12
Identities = 26/84 (30%), Positives = 37/84 (43%), Gaps = 4/84 (4%)
Frame = -3
Query: 232 YTWLSLVQAWVFHHFPGMGSNEVWENYVENQYPRAMLFLP----LSGLGTVDHYINYLDA 287
Y L + W++ HFP + + + Y E PRA +L + G+ T Y DA
Sbjct: 258 YVNLIFL*CWIYEHFPSVHQCVIDDTY*ETS-PRASRWLTSKAHMKGI-TGAPYRARCDA 85
Query: 288 LDLTRVVMAQYGVHRQARQFEQVS 311
L + V Y HR R FE +S
Sbjct: 84 LTVIDVSWLPYTEHRGVRAFELIS 13
Score = 25.0 bits (53), Expect(3) = 1e-12
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = -2
Query: 158 YLLYLVGITIFTDKS 172
YLL+LVG T+F +KS
Sbjct: 523 YLLHLVGCTLFANKS 479
>BI470376 weakly similar to GP|14090338|dbj P0638D12.5 {Oryza sativa
(japonica cultivar-group)}, partial (5%)
Length = 429
Score = 52.0 bits (123), Expect = 3e-07
Identities = 30/82 (36%), Positives = 44/82 (53%)
Frame = -1
Query: 33 IMIDHGLIYAFVEMWHEETSSFHLPFEEITVTLDDVTYLLHIPIDDMLLSHGFISRDEAV 92
+ ++ L+ AF+E W ET +FHL + E T+TL DV+ LL IP+D L + +
Sbjct: 351 LKVNGALVNAFIERWRPETHTFHLKYGEATITLQDVSVLLGIPVDGRPLIGN--TNIDWF 178
Query: 93 ELMEEYLESDTGDALIEVDKTK 114
EL E L DA I+ + K
Sbjct: 177 ELFHELLGVMPDDAAIDGNSIK 112
>BE475635
Length = 412
Score = 46.6 bits (109), Expect = 1e-05
Identities = 23/61 (37%), Positives = 39/61 (63%)
Frame = +3
Query: 162 LVGITIFTDKSQWAVDVVYLKFFRNIDLVSSFSWGVAALAHLY*ELNNAARWNCSQMAGY 221
L+G T+F +KS V V++L FR++ S++ V AL H+Y LN+A + + ++AGY
Sbjct: 3 LLGCTLFANKSATHVYVIFLDAFRDLSQSGSYA*VVVALVHMYDHLNDACKSDDRKLAGY 182
Query: 222 L 222
+
Sbjct: 183 I 185
>CO979307
Length = 553
Score = 40.0 bits (92), Expect = 0.001
Identities = 35/114 (30%), Positives = 50/114 (43%)
Frame = -3
Query: 161 YLVGITIFTDKSQWAVDVVYLKFFRNIDLVSSFSWGVAALAHLY*ELNNAARWNCSQMAG 220
+L+ IF DKS V V YL + N+ GVAAL +LY L+ + + Q
Sbjct: 551 HLISSMIFADKSLAHVHVAYL*YLSNLTACHK*V*GVAALVYLYDHLSYVSLDDNKQCGD 372
Query: 221 YLTLFVYGLYIYTWLSLVQAWVFHHFPGMGSNEVWENYVENQYPRAMLFLPLSG 274
Y+T L+ A VF H P +G + E+Y + P + PL G
Sbjct: 371 YMT-------------LLMA*VFEHLPSVGYSNP-EDYSDGD-PLNTRWNPLGG 255
>TC211545 weakly similar to UP|Q7XHQ1 (Q7XHQ1) Calcineurin-like
phosphoesterase-like, partial (11%)
Length = 1127
Score = 36.2 bits (82), Expect = 0.020
Identities = 17/46 (36%), Positives = 27/46 (57%)
Frame = +3
Query: 32 YIMIDHGLIYAFVEMWHEETSSFHLPFEEITVTLDDVTYLLHIPID 77
Y+ I+ LI +E +T +FH+ E T+TL DV+ LL + +D
Sbjct: 15 YLKINASLITVLIER*RPKTHTFHMRCRECTITLQDVSVLLGMRVD 152
>AI495080
Length = 407
Score = 33.5 bits (75), Expect = 0.13
Identities = 21/78 (26%), Positives = 39/78 (49%), Gaps = 4/78 (5%)
Frame = +2
Query: 234 WLSLVQAWVFHHFPGMGSNEVWENYVENQYPRAMLFL----PLSGLGTVDHYINYLDALD 289
+++L+Q W++ HFP + + +Y E PRA ++ + + T Y L+ L
Sbjct: 17 YITLLQCWIYEHFPSVAESTAD*DYDEAS-PRACRWIATKKTVKSIRT-PSYRERLNRLR 190
Query: 290 LTRVVMAQYGVHRQARQF 307
++ V YG HR+ + F
Sbjct: 191 ISDVCWILYGEHREVQDF 244
>TC208196
Length = 566
Score = 31.6 bits (70), Expect = 0.48
Identities = 22/81 (27%), Positives = 41/81 (50%), Gaps = 6/81 (7%)
Frame = +1
Query: 82 SHGFISRDEAVELMEEYLESDTGDALI---EVDKTKGAHCRFSYLEKIFKE---CLKEQR 135
S G++ +D E++ +DT +A+I + HC SYLE++ ++ C E+R
Sbjct: 10 SVGYLLKDSQREVV-----ADTVNAMILSANPNMKDSKHCLHSYLERLLRQLTACCLERR 174
Query: 136 DLAAEYGVTEEVERLQDQTVR 156
L E G +++R+ + R
Sbjct: 175 SLNGEQGEAFQLQRVLSSSRR 237
>TC208195
Length = 797
Score = 30.0 bits (66), Expect = 1.4
Identities = 21/81 (25%), Positives = 41/81 (49%), Gaps = 6/81 (7%)
Frame = +2
Query: 82 SHGFISRDEAVELMEEYLESDTGDALI---EVDKTKGAHCRFSYLEKIFKE---CLKEQR 135
S G++ +D E++ +DT +A+I ++ HC SYLE++ ++ C +R
Sbjct: 317 SVGYLLKDSQREVV-----ADTVNAMILSTNLNMKDSKHCLHSYLERLLRQLTACCLVRR 481
Query: 136 DLAAEYGVTEEVERLQDQTVR 156
L E G +++R+ + R
Sbjct: 482 SLNGEQGEAFQLQRVLSSSRR 544
>BE805433
Length = 420
Score = 29.3 bits (64), Expect = 2.4
Identities = 18/68 (26%), Positives = 33/68 (48%)
Frame = +3
Query: 184 FRNIDLVSSFSWGVAALAHLY*ELNNAARWNCSQMAGYLTLFVYGLYIYTWLSLVQAWVF 243
FR +++ S W + + L W+C M L L + L ++ +L +++ +F
Sbjct: 15 FRRLEMK*SSLWTMMVIWPKCILLRRKGEWSCHFMEISLWLDINQLMVHPFL--LRSLLF 188
Query: 244 HHFPGMGS 251
HH P +GS
Sbjct: 189 HHLPILGS 212
>AI959929
Length = 474
Score = 28.9 bits (63), Expect = 3.1
Identities = 16/40 (40%), Positives = 22/40 (55%)
Frame = -1
Query: 43 FVEMWHEETSSFHLPFEEITVTLDDVTYLLHIPIDDMLLS 82
F+ +W E TS FH +EI ++ VTY L +D L S
Sbjct: 243 FISLWIEPTS-FHRMDKEICASISFVTYRLRYGLDTYLKS 127
>BG839790 similar to PIR|D96521|D96 protein F21D18.16 [imported] -
Arabidopsis thaliana, partial (2%)
Length = 504
Score = 28.1 bits (61), Expect = 5.3
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -1
Query: 31 SYIMIDHGLIYAFVEMWHEETSSFHL 56
SY+ I+ L+ +E W ET +FH+
Sbjct: 222 SYLKINASLMTVLIERWRPETHTFHM 145
>AI899862
Length = 428
Score = 28.1 bits (61), Expect = 5.3
Identities = 16/38 (42%), Positives = 19/38 (49%), Gaps = 2/38 (5%)
Frame = -2
Query: 18 RLHGTDL--GWLQDTSYIMIDHGLIYAFVEMWHEETSS 53
RLH T L GW +D Y I HG + FVE + S
Sbjct: 127 RLHLTRLISGW*RDERYDAIRHGRFFYFVEQYRSSDLS 14
>TC204009 homologue to UP|Q9XQB6 (Q9XQB6) Chlorophyll a/b-binding protein
CP24, complete
Length = 1041
Score = 28.1 bits (61), Expect = 5.3
Identities = 35/140 (25%), Positives = 57/140 (40%), Gaps = 19/140 (13%)
Frame = +3
Query: 177 DVVYLKFFRNIDLVSSFSWGVAALAHLY*-ELNNAARW-NCSQMAGYLTLFVYGLYIYTW 234
D +LK++R +L+ W +AA+ ++ + + W + F +G + T
Sbjct: 345 DPAFLKWYREAELIHG-RWAMAAVLGIFVGQAWSGVPWFEAGADPNAIAPFSFGTLLGTQ 521
Query: 235 LSLV-----QAWVFHHFPGMGSNEVW--------ENYV----ENQYPRAMLFLPLSGLGT 277
L L+ + WV P S E W EN+ E YP F PLS GT
Sbjct: 522 LILMGWVESKRWVDFFNPDSQSVE-WATPWSRTSENFANATGEQGYPGGKFFDPLSLAGT 698
Query: 278 VDHYINYLDALDLTRVVMAQ 297
+ + D L R+ +A+
Sbjct: 699 IKDGVYIPDTEKLERLKLAE 758
>AI973900
Length = 209
Score = 27.3 bits (59), Expect = 9.1
Identities = 16/48 (33%), Positives = 24/48 (49%), Gaps = 1/48 (2%)
Frame = +1
Query: 117 HCRFSYLEKIF-KECLKEQRDLAAEYGVTEEVERLQDQTVRIYLLYLV 163
H +F+ L +F K C E + +T ++ R QTV I LLY +
Sbjct: 52 HTQFTILPILFSKVCYVENIS*LSMQSITNQINRQYMQTVLIVLLYFI 195
>TC218035
Length = 478
Score = 27.3 bits (59), Expect = 9.1
Identities = 9/22 (40%), Positives = 15/22 (67%)
Frame = -3
Query: 35 IDHGLIYAFVEMWHEETSSFHL 56
IDHG +Y+ + W ++ + FHL
Sbjct: 398 IDHGSVYSTFQTW*QKDAEFHL 333
>TC211576 weakly similar to UP|Q7Q720 (Q7Q720) EbiP7196 (Fragment), partial
(3%)
Length = 392
Score = 27.3 bits (59), Expect = 9.1
Identities = 18/60 (30%), Positives = 30/60 (50%)
Frame = -1
Query: 55 HLPFEEITVTLDDVTYLLHIPIDDMLLSHGFISRDEAVELMEEYLESDTGDALIEVDKTK 114
+LPF ++TV D DD L +HGF+S +L L++ + +A V+ T+
Sbjct: 311 NLPFPDVTVPAHD---------DDFLPAHGFVSLSSNSDLEHLDLKNRSPEAEEVVEATE 159
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.326 0.140 0.445
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,317,488
Number of Sequences: 63676
Number of extensions: 225652
Number of successful extensions: 1334
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1324
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1332
length of query: 311
length of database: 12,639,632
effective HSP length: 97
effective length of query: 214
effective length of database: 6,463,060
effective search space: 1383094840
effective search space used: 1383094840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 59 (27.3 bits)
Medicago: description of AC144893.1