
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.21 - phase: 0
(471 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC222068 similar to GB|CAA80380.1|1673365|ATTRNASYA mitochondria... 130 1e-30
BG155974 similar to GP|1673365|emb mitochondrial tRNA-Ala synthe... 122 3e-28
AI495972 similar to PIR|D96538|D965 cytosolic tRNA-Ala synthetas... 82 5e-20
BF069523 similar to GP|4689382|gb|A chlorophyll a/b binding prot... 30 1.8
NP005150 cytokinin induced message 30 1.8
TC211784 30 1.8
TC215036 similar to PIR|T06397|T06397 isoprenylated protein FP6 ... 29 4.0
TC203437 homologue to UP|RCA_CUCSA (Q01587) Ribulose bisphosphat... 29 5.2
TC220518 28 6.8
TC213750 similar to UP|Q9W3W8 (Q9W3W8) CG3203-PA (Cg3203-pb) (GM... 28 6.8
BU547384 28 8.8
>TC222068 similar to GB|CAA80380.1|1673365|ATTRNASYA mitochondrial tRNA-Ala
synthetase {Arabidopsis thaliana;} , partial (19%)
Length = 600
Score = 130 bits (328), Expect = 1e-30
Identities = 67/101 (66%), Positives = 76/101 (74%)
Frame = +1
Query: 362 LGDVFPELKQNEERIRKMIQVEEESFSRNLFKGIRKFNKAVEHVQGNILSGEATFELSHT 421
+GDVFPELKQ E IR +I+ EEESF R L KGI KF AV VQGNILSGE F L T
Sbjct: 7 MGDVFPELKQQETHIRNIIEEEEESFGRTLIKGIEKFEAAVRQVQGNILSGEEAFVLWDT 186
Query: 422 FGFPLDLTQLMAEEKKFSVDIEGFHRAMKAARERSKTAPKK 462
+GFPLDLTQLMAEEK VD++GF AM+AARERS++A K
Sbjct: 187 YGFPLDLTQLMAEEKGLVVDVKGFDSAMEAARERSRSAQTK 309
>BG155974 similar to GP|1673365|emb mitochondrial tRNA-Ala synthetase
{Arabidopsis thaliana}, partial (8%)
Length = 381
Score = 122 bits (307), Expect = 3e-28
Identities = 58/75 (77%), Positives = 64/75 (85%)
Frame = +1
Query: 1 MPETESIDIEWPAKRVRDTFAKFFEEKNHVHWKSSAVVPLNDPTLLFANAGMNQFKPIFL 60
MPETE+ D+EWPAKRVRDTF FF++KNHV+WKSS VVP NDPTLLFANAGMNQFKPIFL
Sbjct: 94 MPETETHDVEWPAKRVRDTFISFFQDKNHVNWKSSPVVPFNDPTLLFANAGMNQFKPIFL 273
Query: 61 GTVDPNTALSKLTRA 75
GT LSKL+RA
Sbjct: 274 GTGGS*HCLSKLSRA 318
Score = 47.0 bits (110), Expect = 2e-05
Identities = 21/30 (70%), Positives = 22/30 (73%)
Frame = +3
Query: 67 TALSKLTRACNIQKCIRAGGKHNDLDDVGK 96
T L + N QKCIRAGGKHNDLDDVGK
Sbjct: 291 TLLEQALSCLNTQKCIRAGGKHNDLDDVGK 380
>AI495972 similar to PIR|D96538|D965 cytosolic tRNA-Ala synthetase [imported]
- Arabidopsis thaliana, partial (6%)
Length = 180
Score = 81.6 bits (200), Expect(2) = 5e-20
Identities = 36/45 (80%), Positives = 39/45 (86%)
Frame = +1
Query: 134 LPSDRIYVTYFGGDEKTGLGPDNEARDIWLKFLPPGRVLPFGSKD 178
+ SDRIY TYFGGD+K+GL PD EARDIWLKFLPPGRVLPF KD
Sbjct: 46 ITSDRIYATYFGGDDKSGLAPDLEARDIWLKFLPPGRVLPFDCKD 180
Score = 34.3 bits (77), Expect(2) = 5e-20
Identities = 14/16 (87%), Positives = 15/16 (93%)
Frame = +2
Query: 119 EAISWAWELLTEVYNL 134
EAISWAWELLT+VY L
Sbjct: 2 EAISWAWELLTKVYKL 49
>BF069523 similar to GP|4689382|gb|A chlorophyll a/b binding protein CP29
{Vigna radiata}, partial (20%)
Length = 190
Score = 30.4 bits (67), Expect = 1.8
Identities = 14/24 (58%), Positives = 15/24 (62%)
Frame = -2
Query: 157 EARDIWLKFLPPGRVLPFGSKDNF 180
EAR W K LPPG L FGS+ F
Sbjct: 156 EARSCWSK*LPPGYSLFFGSRSEF 85
>NP005150 cytokinin induced message
Length = 834
Score = 30.4 bits (67), Expect = 1.8
Identities = 18/76 (23%), Positives = 37/76 (48%)
Frame = +1
Query: 189 CGPCSEIHFDRIGNRNASSFVNDEDPNCIELWNLVFIQFNREADGSLKSLPAKHVDTGMG 248
CG ++ HFD GN + ++ +D N + E + +S+ A HVD+G
Sbjct: 382 CGSDAQYHFDLSGNAFGAMAISGQDENLRNAGKINIQHRRIECNYPGRSI-AFHVDSGSN 558
Query: 249 FERLTSILQHKNSNYD 264
E +++++++ + D
Sbjct: 559 QEYFATLVEYEDGDGD 606
>TC211784
Length = 925
Score = 30.4 bits (67), Expect = 1.8
Identities = 11/29 (37%), Positives = 17/29 (57%)
Frame = -3
Query: 116 FKEEAISWAWELLTEVYNLPSDRIYVTYF 144
F E + W+W + E YN+ SD Y ++F
Sbjct: 164 FSEGLLDWSWLIYIEEYNIDSDYSYNSFF 78
>TC215036 similar to PIR|T06397|T06397 isoprenylated protein FP6 - soybean
(fragment) {Glycine max;} , partial (28%)
Length = 639
Score = 29.3 bits (64), Expect = 4.0
Identities = 12/28 (42%), Positives = 18/28 (63%)
Frame = +1
Query: 52 MNQFKPIFLGTVDPNTALSKLTRACNIQ 79
M K I LG +DP +A+SKL + C+ +
Sbjct: 193 MKDMKMIVLGDIDPVSAVSKLRKCCHTE 276
>TC203437 homologue to UP|RCA_CUCSA (Q01587) Ribulose bisphosphate
carboxylase/oxygenase activase, chloroplast precursor
(RuBisCO activase) (RA) , partial (14%)
Length = 693
Score = 28.9 bits (63), Expect = 5.2
Identities = 19/63 (30%), Positives = 31/63 (49%)
Frame = -3
Query: 367 PELKQNEERIRKMIQVEEESFSRNLFKGIRKFNKAVEHVQGNILSGEATFELSHTFGFPL 426
P+ + ER RK ++ + + N KGI ++N + V+ L +L HT+ L
Sbjct: 475 PKEMADHERRRKAMKRQRGAMHTNQQKGIHRYNFESDFVEYGDLDDVILHDLFHTY---L 305
Query: 427 DLT 429
DLT
Sbjct: 304 DLT 296
>TC220518
Length = 458
Score = 28.5 bits (62), Expect = 6.8
Identities = 20/76 (26%), Positives = 35/76 (45%)
Frame = +2
Query: 350 FFNGLVNVVANVLGDVFPELKQNEERIRKMIQVEEESFSRNLFKGIRKFNKAVEHVQGNI 409
F N ++N+V +VL + F Q+ + +E S++L KG+ V+ V
Sbjct: 50 FCNEVLNIVDDVLDEAFNFFYQDAST---KLNIEGTQRSQDLKKGVDCVRLNVQSVLDKQ 220
Query: 410 LSGEATFELSHTFGFP 425
L+ ++ L H F P
Sbjct: 221 LAAWESYILRHCFALP 268
>TC213750 similar to UP|Q9W3W8 (Q9W3W8) CG3203-PA (Cg3203-pb) (GM02242p),
partial (94%)
Length = 628
Score = 28.5 bits (62), Expect = 6.8
Identities = 23/80 (28%), Positives = 38/80 (46%), Gaps = 4/80 (5%)
Frame = +2
Query: 333 LRRAVRYGREVLKAEEGF----FNGLVNVVANVLGDVFPELKQNEERIRKMIQVEEESFS 388
LRRAVRY + V + +E FNG V A + + ++ +IQ+ + + S
Sbjct: 194 LRRAVRYLKNVTEKKECVPFRRFNGGVGRCAQAKAWKTTQGRWPKKSAEFLIQLLKNAES 373
Query: 389 RNLFKGIRKFNKAVEHVQGN 408
+KG+ +EH+Q N
Sbjct: 374 NADYKGLDVDRLVIEHIQVN 433
>BU547384
Length = 537
Score = 28.1 bits (61), Expect = 8.8
Identities = 15/45 (33%), Positives = 21/45 (46%)
Frame = +3
Query: 94 VGKDTYHHTFFEMLGNWSFGDYFKEEAISWAWELLTEVYNLPSDR 138
V D + F LG+ GD++ + W W LT +LPS R
Sbjct: 375 VAIDCHMFIFGGRLGSQRLGDFWVLDTDIWQWSELTGFGDLPSPR 509
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.139 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,086,062
Number of Sequences: 63676
Number of extensions: 298403
Number of successful extensions: 1248
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 1245
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1247
length of query: 471
length of database: 12,639,632
effective HSP length: 101
effective length of query: 370
effective length of database: 6,208,356
effective search space: 2297091720
effective search space used: 2297091720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC144765.21