BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.13 + phase: 0
(146 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC210240 weakly similar to GB|AAT41846.1|48310596|BT014863 At3g0... 185 6e-48
BE807325 36 0.006
TC209635 weakly similar to UP|Q7QF03 (Q7QF03) AgCP13350 (Fragmen... 28 1.6
BE058371 28 1.6
TC230473 28 1.6
BE807908 similar to GP|21666454|g large polyprotein 2 {Bean pod ... 27 3.5
TC204376 similar to UP|Q9SVG3 (Q9SVG3) Reticuline oxidase-like p... 27 3.5
TC215504 homologue to UP|Q94AF6 (Q94AF6) AT5g20160/F5O24_50 (Rib... 27 4.6
TC227625 27 4.6
TC226331 UP|Q6R269 (Q6R269) Disease resistance protein, complete 27 4.6
TC228720 weakly similar to UP|RAD5_YEAST (P32849) DNA repair pro... 27 4.6
AW832288 26 6.0
TC228853 similar to UP|O04082 (O04082) Transcription factor RUSH... 26 6.0
TC234578 similar to UP|PHSH_VICFA (P53537) Alpha-glucan phosphor... 26 6.0
AW704348 26 6.0
TC233942 26 7.8
TC227606 similar to UP|Q8RX56 (Q8RX56) AT5g06970/MOJ9_14, partia... 26 7.8
>TC210240 weakly similar to GB|AAT41846.1|48310596|BT014863 At3g07860
{Arabidopsis thaliana;} , partial (91%)
Length = 820
Score = 185 bits (470), Expect = 6e-48
Identities = 94/128 (73%), Positives = 110/128 (85%), Gaps = 3/128 (2%)
Frame = +3
Query: 8 KSGLKKAKV---LKALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDV 64
KS +KKA++ L LL+DPIL+DVPKNPT DV+TLI LELGSAMRI+VLKLD T+ DV
Sbjct: 150 KSSVKKARLNSLLTTLLDDPILSDVPKNPTFADVDTLINLELGSAMRISVLKLDGTTLDV 329
Query: 65 ILMNTATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDVLQNL 124
I+MN+AT+KDLKLAIKKKVN MEQSSMGHRHISW+ VWANYCLS NN LL+D++ L+N
Sbjct: 330 IVMNSATVKDLKLAIKKKVNDMEQSSMGHRHISWKHVWANYCLSCHNN*LLDDNEALRNF 509
Query: 125 GVRNNSQV 132
GVRNNSQV
Sbjct: 510 GVRNNSQV 533
>BE807325
Length = 456
Score = 36.2 bits (82), Expect = 0.006
Identities = 13/45 (28%), Positives = 25/45 (54%)
Frame = +3
Query: 92 GHRHISWRSVWANYCLSFDNNKLLNDDDVLQNLGVRNNSQVSSTY 136
G I W VW +CL + KL+++ D +++ G+++ Q+ Y
Sbjct: 24 GPGKILWPLVWRQFCLCYQGQKLVSEMDYIRDYGIKDGDQLHFVY 158
>TC209635 weakly similar to UP|Q7QF03 (Q7QF03) AgCP13350 (Fragment), partial
(16%)
Length = 921
Score = 28.1 bits (61), Expect = 1.6
Identities = 9/24 (37%), Positives = 14/24 (57%)
Frame = +1
Query: 122 QNLGVRNNSQVSSTYLCHCWNCEI 145
+ LG + S+ CHCWNC++
Sbjct: 16 RGLGNSHGLSTFSSEYCHCWNCKL 87
>BE058371
Length = 434
Score = 28.1 bits (61), Expect = 1.6
Identities = 12/37 (32%), Positives = 19/37 (50%), Gaps = 4/37 (10%)
Frame = -2
Query: 75 LKLAIKKKVNYMEQ----SSMGHRHISWRSVWANYCL 107
LKL + K + + + +G I W +WA+YCL
Sbjct: 133 LKLVVSYKHSSQHECRCSAELGSHGIRWEEIWADYCL 23
>TC230473
Length = 955
Score = 28.1 bits (61), Expect = 1.6
Identities = 12/37 (32%), Positives = 19/37 (50%), Gaps = 4/37 (10%)
Frame = -1
Query: 75 LKLAIKKKVNYMEQ----SSMGHRHISWRSVWANYCL 107
LKL + K + + + +G I W +WA+YCL
Sbjct: 571 LKLVVSYKHSSQHECRCSAELGSHGIRWEEIWADYCL 461
>BE807908 similar to GP|21666454|g large polyprotein 2 {Bean pod mottle
virus}, partial (19%)
Length = 601
Score = 26.9 bits (58), Expect = 3.5
Identities = 17/56 (30%), Positives = 24/56 (42%), Gaps = 2/56 (3%)
Frame = -1
Query: 86 MEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDV--LQNLGVRNNSQVSSTYLCH 139
+ Q S G H+SW S F N LN V L+ +R+N +S C+
Sbjct: 229 ISQQSCGRTHLSWPSCKKVLM*KFQYNFFLNLSTVRQLERKSLRSNGVISQISKCY 62
>TC204376 similar to UP|Q9SVG3 (Q9SVG3) Reticuline oxidase-like protein,
partial (63%)
Length = 1418
Score = 26.9 bits (58), Expect = 3.5
Identities = 16/58 (27%), Positives = 24/58 (40%)
Frame = +3
Query: 84 NYMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDVLQNLGVRNNSQVSSTYLCHCW 141
N+ + G SW + W + +LQNLG +S +SS + HCW
Sbjct: 72 NHRGRKKRGCSGRSWSNTWRS---------------LLQNLGEE*SSWLSSRGVSHCW 200
>TC215504 homologue to UP|Q94AF6 (Q94AF6) AT5g20160/F5O24_50 (Ribosomal
protein L7Ae-like), complete
Length = 680
Score = 26.6 bits (57), Expect = 4.6
Identities = 14/38 (36%), Positives = 19/38 (49%)
Frame = -3
Query: 107 LSFDNNKLLNDDDVLQNLGVRNNSQVSSTYLCHCWNCE 144
+ F N +LLN L NLG S +S C+ W C+
Sbjct: 459 IKFSN*QLLNSIL*LLNLGFELTSFISCYRTCNHWPCD 346
>TC227625
Length = 1751
Score = 26.6 bits (57), Expect = 4.6
Identities = 8/25 (32%), Positives = 18/25 (72%)
Frame = +1
Query: 96 ISWRSVWANYCLSFDNNKLLNDDDV 120
++W S+ +++CL FD ++DD++
Sbjct: 1624 LNWGSIISSWCLRFDVQNAIDDDNM 1698
>TC226331 UP|Q6R269 (Q6R269) Disease resistance protein, complete
Length = 4194
Score = 26.6 bits (57), Expect = 4.6
Identities = 29/122 (23%), Positives = 56/122 (45%), Gaps = 15/122 (12%)
Frame = +3
Query: 33 PTLEDVETLIGLELGSAMRITVLKLDTTS----FDVILMNTATLKDLK---LAIKKKVNY 85
PT+ +ETL G G+ +++ KLD+ F ++ LK LK +++ V+
Sbjct: 63 PTMPVLETLGGALFGAVLQVLFDKLDSHQVLDYFRGRKLDGRLLKTLKWKLMSVNAVVDD 242
Query: 86 MEQSSMGHRHI-SWRSVWANYCLSFDNNKLLNDDDVLQNLG-------VRNNSQVSSTYL 137
EQ +++ W L + LLN +D+L+ + ++ SQ S++ +
Sbjct: 243 AEQKQFTDKNVKEW--------LDEVRDVLLNTEDLLEEIDYEFTKTELKAESQTSASKV 398
Query: 138 CH 139
C+
Sbjct: 399 CN 404
>TC228720 weakly similar to UP|RAD5_YEAST (P32849) DNA repair protein RAD5,
partial (4%)
Length = 736
Score = 26.6 bits (57), Expect = 4.6
Identities = 19/68 (27%), Positives = 31/68 (44%)
Frame = +3
Query: 25 ILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDVILMNTATLKDLKLAIKKKVN 84
++ D+ NPT ED +G +TVL+L T++D LA+++K
Sbjct: 123 LMLDLWWNPTTEDQAIDRAHRIGQTRPVTVLRLTVRD---------TVEDRILALQQKKR 275
Query: 85 YMEQSSMG 92
M S+ G
Sbjct: 276 MMVASAFG 299
>AW832288
Length = 421
Score = 26.2 bits (56), Expect = 6.0
Identities = 11/32 (34%), Positives = 16/32 (49%)
Frame = +1
Query: 114 LLNDDDVLQNLGVRNNSQVSSTYLCHCWNCEI 145
+L+ D N VRNN+ + CH N E+
Sbjct: 112 ILDSSDYEDNNSVRNNNSIKLNKQCHFSNTEV 207
>TC228853 similar to UP|O04082 (O04082) Transcription factor RUSH-1alpha
isolog; 18684-24052, partial (24%)
Length = 1393
Score = 26.2 bits (56), Expect = 6.0
Identities = 19/68 (27%), Positives = 31/68 (44%)
Frame = +2
Query: 25 ILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDVILMNTATLKDLKLAIKKKVN 84
++ D+ NPT ED +G +TVL+L T++D LA+++K
Sbjct: 935 LMLDLWWNPTTEDQAIDRAHRIGQTRPVTVLRLTVRD---------TVEDRILALQQKKR 1087
Query: 85 YMEQSSMG 92
M S+ G
Sbjct: 1088TMVASAFG 1111
>TC234578 similar to UP|PHSH_VICFA (P53537) Alpha-glucan phosphorylase, H
isozyme (Starch phosphorylase H) , partial (4%)
Length = 519
Score = 26.2 bits (56), Expect = 6.0
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = -3
Query: 113 KLLNDDDVLQNLGVRNNSQVSSTYLCHCWN 142
K+ + ++ N+G N ++ S ++CHCWN
Sbjct: 517 KMRTELSMVTNIGC-NLIRLFSQWMCHCWN 431
>AW704348
Length = 364
Score = 26.2 bits (56), Expect = 6.0
Identities = 11/29 (37%), Positives = 18/29 (61%)
Frame = +3
Query: 88 QSSMGHRHISWRSVWANYCLSFDNNKLLN 116
+S H ++S+ WA+ CL++ N K LN
Sbjct: 171 ESGRYHLYVSYACPWASRCLAYLNIKGLN 257
>TC233942
Length = 454
Score = 25.8 bits (55), Expect = 7.8
Identities = 13/32 (40%), Positives = 18/32 (55%), Gaps = 2/32 (6%)
Frame = -1
Query: 116 NDDDVLQNLGVRNNSQVSSTYL--CHCWNCEI 145
N ++V+ L R + QVS TYL H W E+
Sbjct: 301 NREEVVVTLYCRQDPQVSYTYLQFVHAWQAEL 206
>TC227606 similar to UP|Q8RX56 (Q8RX56) AT5g06970/MOJ9_14, partial (36%)
Length = 1540
Score = 25.8 bits (55), Expect = 7.8
Identities = 16/41 (39%), Positives = 22/41 (53%)
Frame = -1
Query: 97 SWRSVWANYCLSFDNNKLLNDDDVLQNLGVRNNSQVSSTYL 137
+WR+ LS NKL N +++ L +N S VSST L
Sbjct: 295 TWRA------LSMPRNKLFNSVNLIGTLRPKN*STVSSTIL 191
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.133 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,514,772
Number of Sequences: 63676
Number of extensions: 79966
Number of successful extensions: 644
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 643
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 644
length of query: 146
length of database: 12,639,632
effective HSP length: 88
effective length of query: 58
effective length of database: 7,036,144
effective search space: 408096352
effective search space used: 408096352
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)
Medicago: description of AC144765.13