BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144730.8 - phase: 0
(840 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC220322 101 1e-32
BM308209 65 3e-20
BF425842 80 5e-15
TC210254 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F1... 75 9e-14
TC222000 weakly similar to PIR|H84710|H84710 Mutator-like transp... 70 3e-12
BE806360 similar to GP|6175165|gb| Mutator-like transposase {Ara... 49 7e-06
TC220753 48 2e-05
TC232149 47 3e-05
AI856054 39 0.001
TC214000 41 0.003
BM108247 weakly similar to PIR|H84710|H847 Mutator-like transpos... 40 0.004
TC219010 homologue to UP|Q9AXI4 (Q9AXI4) APETAL2-like protein, p... 33 0.40
TC207649 similar to UP|Q9SY66 (Q9SY66) F14N23.12 (At1g10240/F14N... 33 0.68
BI701322 similar to GP|9988432|dbj| P0671B11.14 {Oryza sativa (j... 32 1.2
TC227468 similar to UP|Q8H1D0 (Q8H1D0) Transcription factor MYBS... 32 1.2
BI893202 32 1.5
TC230490 similar to UP|Q8LEE4 (Q8LEE4) Zinc finger protein, part... 31 2.0
AI960729 similar to GP|21593313|gb| protein disulfide isomerase ... 31 2.6
AW780793 31 2.6
TC212938 30 3.4
>TC220322
Length = 1264
Score = 101 bits (251), Expect(2) = 1e-32
Identities = 57/161 (35%), Positives = 85/161 (52%), Gaps = 2/161 (1%)
Frame = +2
Query: 579 MNELKKIDLGAWEWLMAVEPKKWCKHAFTFYSKCDVLMNNISESFNVTILSARDQPIISM 638
M LK I+ AWE+L + W K F+ K D + NN E FN IL R +PII+M
Sbjct: 44 MAHLKTINCQAWEYLNKWPKQAWTKAHFSTIPKVDNICNNTCEVFNFRILQYRCKPIITM 223
Query: 639 AEWIRHYLMRRMTTSATKLQKWQHNVMPMPRKRLDKEITLAAHWKSTWSG--IGEQFQVM 696
E IR Y+MR M KL + + KRL+KE A W W G +G +++V
Sbjct: 224 LEEIRSYIMRTMAARKVKLSGKPGPLCLVQYKRLEKEFHFANQWTPIWCGDNMGLRYEVH 403
Query: 697 HTYNRQQFIVDIAKRSCSCNFWEIVGIPCRHVVSALGKRKQ 737
N+ + V++A +C+C W++ G+PCRH ++ + K+
Sbjct: 404 MWGNKVE--VNLAGWTCTCGVWQLTGMPCRHAIATITLTKE 520
Score = 58.2 bits (139), Expect(2) = 1e-32
Identities = 29/94 (30%), Positives = 46/94 (48%), Gaps = 3/94 (3%)
Frame = +1
Query: 738 RPEMFVDDYYSRSKYALCYSFAISPINGMDMWPEVEAPELLPPNYKNGPGRPRKLRIREF 797
+PE ++ S Y Y I P+ G W + + +PP + GRP+K R R
Sbjct: 523 KPEDMCHEWLSIEAYNKTYQHFIEPVQGPQYWAQTQYTHPVPPYKRVQRGRPKKNRKRSV 702
Query: 798 DEN---GSRMRRQGVAYRCTKCDQFGHNQRRCKS 828
DE+ G +++R+ + C +C Q HN R CK+
Sbjct: 703 DEDNVTGHKLKRKLAEFTCGRCGQTNHNIRSCKN 804
>BM308209
Length = 435
Score = 64.7 bits (156), Expect(2) = 3e-20
Identities = 26/52 (50%), Positives = 41/52 (78%)
Frame = +2
Query: 582 LKKIDLGAWEWLMAVEPKKWCKHAFTFYSKCDVLMNNISESFNVTILSARDQ 633
+K+++ A++WL+ V K W KH F++Y+KCDVLMNN+ ++FN TIL AR++
Sbjct: 122 IKEVNNEAYDWLIKVGTKLWRKHTFSYYTKCDVLMNNLCDAFNSTILCAREK 277
Score = 52.8 bits (125), Expect(2) = 3e-20
Identities = 23/42 (54%), Positives = 31/42 (73%)
Frame = +3
Query: 543 RHLYANFKKKFGGGSQIRDLMMGATKATYNQGWLEKMNELKK 584
RH+Y NFK KF GG+ +R+LMM A KA++ + W EKMN K+
Sbjct: 6 RHIYNNFKNKFVGGTLLRNLMMRAAKASFEEAWDEKMNISKR 131
Score = 45.8 bits (107), Expect = 8e-05
Identities = 23/54 (42%), Positives = 33/54 (60%)
Frame = +3
Query: 679 AAHWKSTWSGIGEQFQVMHTYNRQQFIVDIAKRSCSCNFWEIVGIPCRHVVSAL 732
+ +W +T SG F+V H+ +FIV++ SCS N WEI GI CRH +A+
Sbjct: 276 SGNWFATLSG-HLVFEVSHSMLSDKFIVNLNSWSCS*NLWEITGILCRHACAAI 434
>BF425842
Length = 414
Score = 79.7 bits (195), Expect = 5e-15
Identities = 44/119 (36%), Positives = 60/119 (49%), Gaps = 5/119 (4%)
Frame = +3
Query: 440 FDGCKKGFISACRPFVGVDGCHLKTRYGGQLLIAVGRDPNDQYFPLAFGVVETECKETWK 499
F+ C +GF C+P V VDG L +Y LL A+G+D + FPLAF +VE+E KE W
Sbjct: 45 FNPCIEGF-KYCKPLVQVDGTFLTGKYNDTLLTAIGQDGSRDNFPLAFAIVESETKEAWI 221
Query: 500 WFTQLLMEDIGEDKRYVFISDQQKGLLSVFDEMF-----DSIDHRLCLRHLYANFKKKF 553
WF L + ISD+ GLL+ + C+RH+ +NF K F
Sbjct: 222 WFLHYL*RYVTPQPNLCIISDRGTGLLATLQSKHVGWNGPDVSSVYCIRHIASNFNK*F 398
>TC210254 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17A9_9
{Arabidopsis thaliana;} , partial (6%)
Length = 814
Score = 75.5 bits (184), Expect = 9e-14
Identities = 40/128 (31%), Positives = 65/128 (50%), Gaps = 5/128 (3%)
Frame = +1
Query: 705 IVDIAKRSCSCNFWEIVGIPCRHVVSALGKRKQRPEMFVDDYYSRSKYALCYSFAISPIN 764
IVDI CSC W++ G+PC H ++ + P + Y++ Y L Y+ +I P+
Sbjct: 64 IVDIDNWDCSCKGWQLTGVPCCHAIAVFECVGRSPYDYCSRYFTVENYRLTYAESIHPVP 243
Query: 765 GMDMWPEVEAPE-----LLPPNYKNGPGRPRKLRIREFDENGSRMRRQGVAYRCTKCDQF 819
+D P V+ ++PP K PGRP+ ++ D ++RQ +C+KC
Sbjct: 244 NVDK-PPVQGESTALVMVIPPPTKRPPGRPKMKQVESID----IIKRQ---LQCSKCKGL 399
Query: 820 GHNQRRCK 827
GHN++ CK
Sbjct: 400 GHNRKTCK 423
>TC222000 weakly similar to PIR|H84710|H84710 Mutator-like transposase
[imported] - Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (12%)
Length = 617
Score = 70.5 bits (171), Expect = 3e-12
Identities = 42/135 (31%), Positives = 65/135 (48%), Gaps = 7/135 (5%)
Frame = +2
Query: 705 IVDIAKRSCSCNFWEIVGIPCRHVVSALGKRKQRPEMFVDDYYSRSKYALCYSFAISPIN 764
IV+I SCSC W++ GIPC H +AL ++ F ++ + + Y+ I PI
Sbjct: 17 IVNIGSHSCSCRDWQLNGIPCSHAAAALISCRKDVYAFSQKCFTAASFRDTYAETIHPIP 196
Query: 765 GMDMWPEVEAPEL-------LPPNYKNGPGRPRKLRIREFDENGSRMRRQGVAYRCTKCD 817
G W + + PP + PGRP K R+ D N R+ C++C+
Sbjct: 197 GKLEWSKTGNSSMDDNILVVRPPKLRRPPGRPEK-RMCVDDLN-----REKHTVHCSRCN 358
Query: 818 QFGHNQRRCKSDVQN 832
Q GH +R CK+++ N
Sbjct: 359 QTGHYKRTCKAEMIN 403
>BE806360 similar to GP|6175165|gb| Mutator-like transposase {Arabidopsis
thaliana}, partial (17%)
Length = 431
Score = 49.3 bits (116), Expect = 7e-06
Identities = 29/89 (32%), Positives = 45/89 (49%), Gaps = 4/89 (4%)
Frame = +2
Query: 486 AFGVVETECKETWKWFTQLL---MEDIGEDK-RYVFISDQQKGLLSVFDEMFDSIDHRLC 541
AFGVV+ E + W WF L +E E+ R +SD+QKG++ + F + H C
Sbjct: 2 AFGVVDEENDDNWMWFLSELHNLLEIHTENMLRLTILSDRQKGIVDGVEASFPTAFHGFC 181
Query: 542 LRHLYANFKKKFGGGSQIRDLMMGATKAT 570
++HL +F+K+F + L A T
Sbjct: 182 MQHLSDSFRKEFNNTMLVNLLWEAANALT 268
>TC220753
Length = 1312
Score = 48.1 bits (113), Expect = 2e-05
Identities = 31/118 (26%), Positives = 48/118 (40%), Gaps = 4/118 (3%)
Frame = +2
Query: 713 CSCNFWEIVGIPCRHVVSALGKRKQRPEMFVDDYYSRSKYALCYSFAISPINGMDMWPEV 772
C ++ + +PC H ++A + FVD Y Y + D WP+
Sbjct: 236 CDYGQFQALRLPCSHAIAACAFCNLNYDDFVDPVYKLENIFKVYQHHFHSLGSEDTWPQY 415
Query: 773 EAPELL--PPNYKNGPGRPRKLRI-REFDEN-GSRMRRQGVAYRCTKCDQFGHNQRRC 826
P + P + GRP RI E DE+ +R++ +C+ C GHNQ C
Sbjct: 416 LGPHFMSDPSKRRQISGRPATTRIHNEMDESIPNRLK------KCSLCKTEGHNQSNC 571
>TC232149
Length = 1005
Score = 47.4 bits (111), Expect = 3e-05
Identities = 36/143 (25%), Positives = 59/143 (41%), Gaps = 32/143 (22%)
Frame = -3
Query: 635 IISMAEWIRHYLMRRMTTSATKLQKWQHNVMPMPRKRLDKEITLAAHWKSTWSG--IGEQ 692
I++M E IR Y+M +M + K+ ++PM + R++K + + W G G +
Sbjct: 427 ILNMCEDIRTYIMCKMKSDKLKMATRLRPLVPMQQSRIEKGKVESNK*TANWVGDPDGTR 248
Query: 693 FQVMHTYNRQQFIVDIAKRSCSCNFWEIVGI----------------------------- 723
F+V H R VD+ S +C W+++GI
Sbjct: 247 FKVCHYDTRLN--VDLQN*SFTCRMWQLIGIPLS*LNLSILDIMYCVTMNLMFFWFIYIG 74
Query: 724 -PCRHVVSALGKRKQRPEMFVDD 745
PCRH + A+ PE + DD
Sbjct: 73 MPCRHAIVAIRYNYHIPEGYSDD 5
>AI856054
Length = 173
Score = 38.5 bits (88), Expect(2) = 0.001
Identities = 21/47 (44%), Positives = 30/47 (63%)
Frame = -1
Query: 619 ISESFNVTILSARDQPIISMAEWIRHYLMRRMTTSATKLQKWQHNVM 665
I ESFN IL AR++ I+SM E I YLM R T++ K+ + +V+
Sbjct: 143 ICESFNSVILEAREKLIVSMLEDIHIYLMERWDTNSKKVVAREGSVL 3
Score = 22.7 bits (47), Expect(2) = 0.001
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3
Query: 610 SKCDVLMNNI 619
SKCDVL+NN+
Sbjct: 168 SKCDVLLNNM 139
>TC214000
Length = 659
Score = 40.8 bits (94), Expect = 0.003
Identities = 20/52 (38%), Positives = 30/52 (57%), Gaps = 2/52 (3%)
Frame = +3
Query: 791 KLRIREFDE--NGSRMRRQGVAYRCTKCDQFGHNQRRCKSDVQNPEAAKRKV 840
+LR RE DE N ++R C +C + GHN+R CK+ +P+ AK +V
Sbjct: 6 RLRRREVDED*NDHKLRGTNTTN*CWRCHKLGHNKRTCKNSPIHPKPAKTRV 161
>BM108247 weakly similar to PIR|H84710|H847 Mutator-like transposase
[imported] - Arabidopsis thaliana, partial (4%)
Length = 265
Score = 40.0 bits (92), Expect = 0.004
Identities = 27/82 (32%), Positives = 43/82 (51%)
Frame = +2
Query: 557 SQIRDLMMGATKATYNQGWLEKMNELKKIDLGAWEWLMAVEPKKWCKHAFTFYSKCDVLM 616
S++ L+ A+ AT + EKM E++++ A +WL P +W F ++ L
Sbjct: 8 SRLVHLLWKASHATTTIAFKEKMGEIEEVSPEAAKWLQQFXPSQWALVHFK-GTRFGHLS 184
Query: 617 NNISESFNVTILSARDQPIISM 638
+NI E FN IL R+ PII +
Sbjct: 185 SNI-EEFNKWILDTRELPIIXV 247
>TC219010 homologue to UP|Q9AXI4 (Q9AXI4) APETAL2-like protein, partial (35%)
Length = 766
Score = 33.5 bits (75), Expect = 0.40
Identities = 21/64 (32%), Positives = 29/64 (44%)
Frame = +2
Query: 120 YDEEDVMVSTDDDSGKFVGFDDSEDESTTALEDGFEDVEVEAPANGNNRVTVNNKTLRIK 179
Y+ E S +DD GK VG + S +EDG E+ E N N + +R+
Sbjct: 29 YESEGCSSSLNDDKGKRVGSASNSSSSAVVVEDGSEE-EXXXRXXXQNIXQENQQNIRL- 202
Query: 180 KCGS 183
CGS
Sbjct: 203 -CGS 211
>TC207649 similar to UP|Q9SY66 (Q9SY66) F14N23.12 (At1g10240/F14N23_12),
partial (30%)
Length = 971
Score = 32.7 bits (73), Expect = 0.68
Identities = 27/90 (30%), Positives = 42/90 (46%), Gaps = 12/90 (13%)
Frame = +2
Query: 666 PMPRKRLDKEITLAAHWKSTWSGIGEQFQVMHT----------YNRQQFIVDIAKRSCSC 715
P +L +++ LAAH+ S I + F V H + Q+ I+ SCSC
Sbjct: 281 PFAFSKLQEQLVLAAHYASF--SIEDGFLVRHHTKAEGGRKVYWAPQEGII-----SCSC 439
Query: 716 NFWEIVGIPCRHVVSAL--GKRKQRPEMFV 743
+ +E GI CRH + L G Q P+ ++
Sbjct: 440 HQFEFTGILCRHSLRVLSTGNCFQIPDRYL 529
>BI701322 similar to GP|9988432|dbj| P0671B11.14 {Oryza sativa (japonica
cultivar-group)}, partial (12%)
Length = 421
Score = 32.0 bits (71), Expect = 1.2
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +1
Query: 804 MRRQGVAYRCTKCDQFGHNQRRCKS 828
+ G+A +C+ C GHN R CKS
Sbjct: 43 VENMGIARKCSYCGNLGHNARTCKS 117
>TC227468 similar to UP|Q8H1D0 (Q8H1D0) Transcription factor MYBS3, partial
(56%)
Length = 1404
Score = 32.0 bits (71), Expect = 1.2
Identities = 13/26 (50%), Positives = 15/26 (57%)
Frame = +3
Query: 803 RMRRQGVAYRCTKCDQFGHNQRRCKS 828
R RR G+ RC+ C GHN R C S
Sbjct: 36 RRRRIGMTRRCSHCTNNGHNSRTCPS 113
>BI893202
Length = 430
Score = 31.6 bits (70), Expect = 1.5
Identities = 27/111 (24%), Positives = 41/111 (36%), Gaps = 3/111 (2%)
Frame = +3
Query: 132 DSGKFVGFDDSEDESTTALEDGFEDVEVEAPANGNNRVTVNNKTLRIKKCGSKTPKKKSP 191
DS K D +DE +D + N TV T +KK G PKK+S
Sbjct: 60 DSNKTETLSDKKDEGNVVAVQAQDDTQSTGKQTANADTTV---TPEVKKTGKVVPKKQSK 230
Query: 192 KKNNSGRMNVIAPVSLLKNGKGKYVVDEEIDGD---YLSEELGSSDPDDSN 239
+ R N +E + G+ +++L +SD D +N
Sbjct: 231 TPTSEKRDNTADSSKTETKSDKDDKKEERVTGEKSGAKTDKLKASDKDVTN 383
>TC230490 similar to UP|Q8LEE4 (Q8LEE4) Zinc finger protein, partial (19%)
Length = 574
Score = 31.2 bits (69), Expect = 2.0
Identities = 16/52 (30%), Positives = 23/52 (43%)
Frame = +3
Query: 788 RPRKLRIREFDENGSRMRRQGVAYRCTKCDQFGHNQRRCKSDVQNPEAAKRK 839
RP++ + + M Q C C QFGHN+R C V +KR+
Sbjct: 228 RPQQKNM*KVQR*ALAMEEQ*YTISCKICHQFGHNRRTCPQAVPFAVPSKRR 383
>AI960729 similar to GP|21593313|gb| protein disulfide isomerase
precursor-like {Arabidopsis thaliana}, partial (6%)
Length = 438
Score = 30.8 bits (68), Expect = 2.6
Identities = 24/69 (34%), Positives = 31/69 (44%)
Frame = +2
Query: 111 PSFFDLDVGYDEEDVMVSTDDDSGKFVGFDDSEDESTTALEDGFEDVEVEAPANGNNRVT 170
P FD D G DE DD G F GFD S +E+ E +DV V N V
Sbjct: 254 PDEFDEDDGDDE--------DDFGDFAGFDHSSEEAFKEPEVDDKDVVVLKERNFTT-VV 406
Query: 171 VNNKTLRIK 179
NN+ + ++
Sbjct: 407 ENNRFVMVE 433
>AW780793
Length = 419
Score = 30.8 bits (68), Expect = 2.6
Identities = 19/56 (33%), Positives = 27/56 (47%)
Frame = +3
Query: 261 IGMEFNTLVEFKEAIIEWNVLNGYQIKVLKNESYRVRVECRDQCGYKVLCSKVGDR 316
IGMEFN+ E +E I + G+ +++ N RV G +CSK G R
Sbjct: 138 IGMEFNSREEAREFYIAYGRRIGFTVRIHHNRRSRVN---NQVIGQDFVCSKEGFR 296
>TC212938
Length = 998
Score = 30.4 bits (67), Expect = 3.4
Identities = 22/72 (30%), Positives = 34/72 (46%), Gaps = 1/72 (1%)
Frame = -2
Query: 410 KRVCRDNNVKINVAGPSVTIQLRFGSFYFCFDGCKKG-FISACRPFVGVDGCHLKTRYGG 468
KR R V +++ PSVT G + C G F +C F V CHL + GG
Sbjct: 529 KRYGRSACVCLSMI*PSVTQS--GGLRFICLANTA*GIFHPSCSKFKSVGSCHLGGKLGG 356
Query: 469 QLLIAVGRDPND 480
++ + + +PN+
Sbjct: 355 KMYV*LTLNPNE 320
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.137 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,034,973
Number of Sequences: 63676
Number of extensions: 543546
Number of successful extensions: 2738
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 2671
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2721
length of query: 840
length of database: 12,639,632
effective HSP length: 105
effective length of query: 735
effective length of database: 5,953,652
effective search space: 4375934220
effective search space used: 4375934220
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 63 (28.9 bits)
Medicago: description of AC144730.8