BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144730.5 + phase: 0 /pseudo
(146 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC208963 50 3e-07
BM525744 47 3e-06
BM892478 47 3e-06
TC210791 46 7e-06
AW569215 weakly similar to GP|4914326|gb| F14N23.12 {Arabidopsis... 44 3e-05
TC234242 38 0.002
BE057981 37 0.003
BG882562 37 0.003
AW781991 35 0.010
BM095012 27 2.7
TC210256 similar to UP|Q70I28 (Q70I28) S-receptor kinase-like pr... 27 3.5
TC219063 weakly similar to UP|Q9SSE4 (Q9SSE4) MLP3.11 protein, p... 27 4.6
AW755556 homologue to GP|22335691|dbj cullin-like protein1 {Pisu... 26 6.0
TC230548 weakly similar to UP|Q8LLI4 (Q8LLI4) S-locus receptor-l... 26 6.0
TC212393 homologue to UP|TBG1_ARATH (P38557) Tubulin gamma-1 cha... 26 7.8
TC216451 similar to UP|Q93XR7 (Q93XR7) Fructose-6-phosphate 2-ki... 26 7.8
>TC208963
Length = 1163
Score = 50.4 bits (119), Expect = 3e-07
Identities = 32/87 (36%), Positives = 46/87 (52%)
Frame = +1
Query: 58 NMPKVIVTDRDMSLMKEVANVFPESYAMNCYFHVQANVKQRCVLDCKYPLGKKDGKEVKP 117
+MP+VIVT D++LM V VFP S + C FH+ NVK + CK + K+ +E+
Sbjct: 16 DMPQVIVTVGDIALMSAVQVVFPSSSNLLCRFHINQNVKAK----CKSIVHLKEKQEL-- 177
Query: 118 RDVVNKIMRAWKSMVESPTQELYANAL 144
+M AW +V SP + Y L
Sbjct: 178 ------MMDAWDVVVNSPNEGEYMQRL 240
>BM525744
Length = 411
Score = 47.4 bits (111), Expect = 3e-06
Identities = 18/33 (54%), Positives = 27/33 (81%)
Frame = +3
Query: 1 MDSTYKTNMYKMPMFEVVGVTLTDLTYSVGFGF 33
+D+TYK N Y++P+ E+VGVT T+LT+SV F +
Sbjct: 294 LDNTYKVNRYQLPLLEIVGVTSTELTFSVAFAY 392
>BM892478
Length = 429
Score = 47.0 bits (110), Expect = 3e-06
Identities = 20/83 (24%), Positives = 45/83 (54%)
Frame = +3
Query: 1 MDSTYKTNMYKMPMFEVVGVTLTDLTYSVGFGFETHEKEENFVWVLKMLRKLLTSKMNMP 60
+D++Y+ +Y +P VGV +G E EE+F W+ + + ++ + +P
Sbjct: 174 LDTSYRKTVYLVPFATFVGVNHHKQPVLLGCALIADESEESFTWLFQTWLRAMSGR--LP 347
Query: 61 KVIVTDRDMSLMKEVANVFPESY 83
++ D+D+++ + +A VFP ++
Sbjct: 348 LTVIADQDIAIQRAIAKVFPVTH 416
>TC210791
Length = 456
Score = 45.8 bits (107), Expect = 7e-06
Identities = 23/53 (43%), Positives = 31/53 (58%)
Frame = +1
Query: 50 RKLLTSKMNMPKVIVTDRDMSLMKEVANVFPESYAMNCYFHVQANVKQRCVLD 102
R L+ MP VI+T RD++LM V VFP S + C FH+ NVK + +D
Sbjct: 1 RGLIFKDNEMPPVIITVRDIALMDAVQVVFPSSSNLLCRFHISKNVKAKSQVD 159
>AW569215 weakly similar to GP|4914326|gb| F14N23.12 {Arabidopsis thaliana},
partial (19%)
Length = 425
Score = 43.9 bits (102), Expect = 3e-05
Identities = 31/134 (23%), Positives = 56/134 (41%), Gaps = 2/134 (1%)
Frame = +1
Query: 2 DSTYKTNMYKMPMFEVVGVTLTDLTYSVGFGFETHEKEENFVWVLKMLRKLLTSKMNMPK 61
D+TY+ Y M + +GV +T E ++F W LK + K P+
Sbjct: 19 DTTYRVEAYDMLLGIWLGVDNNGMTCFFSCALLRDENIQSFSWALKAFLGFMKGK--APQ 192
Query: 62 VIVTDRDMSLMKEVANVFPESYAMNCYFHVQANVKQ--RCVLDCKYPLGKKDGKEVKPRD 119
I+TD +M L + +A P++ C +H+ + +L +Y K + + +
Sbjct: 193 TILTDHNMWLKEAIAVELPQTKHAFCIWHILSKFSDWFSLLLGSQYDEWKAEFHRLYNLE 372
Query: 120 VVNKIMRAWKSMVE 133
V W+ MV+
Sbjct: 373 QVEDFEEGWRQMVD 414
>TC234242
Length = 478
Score = 37.7 bits (86), Expect = 0.002
Identities = 17/46 (36%), Positives = 28/46 (59%), Gaps = 5/46 (10%)
Frame = +3
Query: 1 MDSTYKTNMYKMPM-----FEVVGVTLTDLTYSVGFGFETHEKEEN 41
+DSTY+TN Y++P+ + VG+T T +T+S F + E+ N
Sbjct: 159 IDSTYRTNRYRLPLLDFVVLDFVGMTPTGMTFSASFAYVEGERVNN 296
>BE057981
Length = 268
Score = 37.0 bits (84), Expect = 0.003
Identities = 15/41 (36%), Positives = 26/41 (62%)
Frame = +2
Query: 1 MDSTYKTNMYKMPMFEVVGVTLTDLTYSVGFGFETHEKEEN 41
+++ YKTN Y++ + + GVT +T+SVGF + E+ N
Sbjct: 131 INNAYKTNRYRLLLLDFAGVTPARMTFSVGFAYLEGERVNN 253
>BG882562
Length = 416
Score = 37.0 bits (84), Expect = 0.003
Identities = 20/77 (25%), Positives = 38/77 (48%)
Frame = +3
Query: 2 DSTYKTNMYKMPMFEVVGVTLTDLTYSVGFGFETHEKEENFVWVLKMLRKLLTSKMNMPK 61
DST +N Y++P+ VGV + +G G E E ++W+ + +T + P+
Sbjct: 192 DSTCLSNNYEIPLVAFVGVNHHGKSVLLGCGLLADETFETYIWLFRAWLTCMTGR--PPQ 365
Query: 62 VIVTDRDMSLMKEVANV 78
I+T+ ++ +A V
Sbjct: 366 TIITNXCKAMQSAIAEV 416
>AW781991
Length = 419
Score = 35.4 bits (80), Expect = 0.010
Identities = 16/46 (34%), Positives = 24/46 (51%)
Frame = +3
Query: 2 DSTYKTNMYKMPMFEVVGVTLTDLTYSVGFGFETHEKEENFVWVLK 47
D+TY++N Y++P GV G F +E E +FVW+ K
Sbjct: 276 DTTYRSNRYRLPFAFFTGVNHHGQPVLFGCAFLINESEASFVWLFK 413
>BM095012
Length = 421
Score = 27.3 bits (59), Expect = 2.7
Identities = 17/57 (29%), Positives = 27/57 (46%)
Frame = +3
Query: 83 YAMNCYFHVQANVKQRCVLDCKYPLGKKDGKEVKPRDVVNKIMRAWKSMVESPTQEL 139
YAM F V+++V VL + GKK+ +P + + AWK+ E +L
Sbjct: 27 YAMRGQFSVKSDVFSFGVLVLEIVSGKKNTDFYQPNQADDLLSYAWKNWTEQTPLQL 197
>TC210256 similar to UP|Q70I28 (Q70I28) S-receptor kinase-like protein 3,
partial (16%)
Length = 659
Score = 26.9 bits (58), Expect = 3.5
Identities = 16/60 (26%), Positives = 30/60 (49%)
Frame = +2
Query: 80 PESYAMNCYFHVQANVKQRCVLDCKYPLGKKDGKEVKPRDVVNKIMRAWKSMVESPTQEL 139
P YA++ F V+++V V+ + G+K+ P + +N + AW+ +E EL
Sbjct: 50 PPEYAVHGSFSVKSDVFSFGVIVLEIISGRKNRGFCDPHNHLNLLGHAWRLWIEKRPLEL 229
>TC219063 weakly similar to UP|Q9SSE4 (Q9SSE4) MLP3.11 protein, partial (12%)
Length = 864
Score = 26.6 bits (57), Expect = 4.6
Identities = 15/52 (28%), Positives = 25/52 (47%), Gaps = 3/52 (5%)
Frame = -3
Query: 55 SKMNMPKVIVTDRDMSLMKEVANVFPESYAMNCYFHVQANVKQ---RCVLDC 103
S+ + V+ D+ +++ + NV SY +NC H +KQ RC C
Sbjct: 340 SERLLASVVARPIDL*MLRLLINVASRSYHINCSSHCLGLLKQWMNRCCRHC 185
>AW755556 homologue to GP|22335691|dbj cullin-like protein1 {Pisum sativum},
partial (12%)
Length = 442
Score = 26.2 bits (56), Expect = 6.0
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = +3
Query: 83 YAMNCYFHVQANVKQRCVLD 102
+ MNCY ++Q N +R +LD
Sbjct: 276 FNMNCYLYMQTNSLKRSILD 335
>TC230548 weakly similar to UP|Q8LLI4 (Q8LLI4) S-locus receptor-like kinase
RLK14, partial (20%)
Length = 692
Score = 26.2 bits (56), Expect = 6.0
Identities = 17/62 (27%), Positives = 28/62 (44%)
Frame = +3
Query: 83 YAMNCYFHVQANVKQRCVLDCKYPLGKKDGKEVKPRDVVNKIMRAWKSMVESPTQELYAN 142
YA + F ++++V VL + GKK+ + P D N I AW E + +
Sbjct: 225 YAFDGIFSIKSDVFSFGVLLLEIVSGKKNSRLFYPNDYNNLIGHAWMLWKEGNPMQFIDS 404
Query: 143 AL 144
+L
Sbjct: 405 SL 410
>TC212393 homologue to UP|TBG1_ARATH (P38557) Tubulin gamma-1 chain (Gamma-1
tubulin), partial (38%)
Length = 578
Score = 25.8 bits (55), Expect = 7.8
Identities = 8/19 (42%), Positives = 13/19 (68%)
Frame = -3
Query: 86 NCYFHVQANVKQRCVLDCK 104
NC+ H +++QRC DC+
Sbjct: 342 NCFLHHLRDLRQRCSHDCR 286
>TC216451 similar to UP|Q93XR7 (Q93XR7) Fructose-6-phosphate
2-kinase/fructose-2,6-bisphosphatase, partial (62%)
Length = 1595
Score = 25.8 bits (55), Expect = 7.8
Identities = 17/66 (25%), Positives = 28/66 (41%), Gaps = 2/66 (3%)
Frame = -3
Query: 43 VWVLKMLRKLLTSKMNMPKVIVTDRDMSLMKEVANVFPESYAMNCY--FHVQANVKQRCV 100
+W+ ++R LL K N+P + + +A F E Y + + FH + V
Sbjct: 609 IWLFCIIRTLLNFKANIP--------FNYISIIAYCFQEDYLASSFSQFHQHVSSFLATV 454
Query: 101 LDCKYP 106
KYP
Sbjct: 453 CGIKYP 436
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.133 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,881,386
Number of Sequences: 63676
Number of extensions: 66999
Number of successful extensions: 321
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 320
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 320
length of query: 146
length of database: 12,639,632
effective HSP length: 88
effective length of query: 58
effective length of database: 7,036,144
effective search space: 408096352
effective search space used: 408096352
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC144730.5