BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144729.7 - phase: 0
(226 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BM108298 similar to PIR|F96767|F96 proteinase IV F2P9.14 [import... 88 3e-18
CO978618 85 2e-17
TC222979 similar to UP|Q8H0V0 (Q8H0V0) Isp4 like protein, partia... 64 4e-11
TC213087 similar to PIR|E69990|E69990 proteinase IV homolog yteI... 52 3e-07
TC223443 similar to PIR|F96767|F96767 proteinase IV F2P9.14 [imp... 49 2e-06
TC222041 similar to UP|Q8LBQ6 (Q8LBQ6) Isp4-like protein, partia... 45 3e-05
BG839504 similar to GP|20466754|gb Isp4-like protein {Arabidopsi... 41 5e-04
TC213192 similar to PIR|F96767|F96767 proteinase IV F2P9.14 [imp... 40 9e-04
BM085219 weakly similar to GP|9758213|dbj sexual differentiation... 37 0.006
AW310775 weakly similar to PIR|T08925|T08 isp4 protein homolog T... 37 0.010
CO978558 32 0.18
BQ080748 weakly similar to PIR|T08925|T08 isp4 protein homolog T... 32 0.18
TC213712 similar to UP|Q9LWW1 (Q9LWW1) ESTs D25093(R3139) (Gluta... 32 0.31
CK605780 30 0.89
TC217829 similar to UP|Q945L7 (Q945L7) AT4g35240/F23E12_200, par... 30 0.89
TC226087 similar to UP|Q9ZPI6 (Q9ZPI6) AIM1 protein, partial (55%) 29 1.5
TC221865 29 2.0
BI786148 weakly similar to GP|16769440|gb LD31383p {Drosophila m... 27 7.5
TC223162 27 7.5
>BM108298 similar to PIR|F96767|F96 proteinase IV F2P9.14 [imported] -
Arabidopsis thaliana, partial (17%)
Length = 509
Score = 88.2 bits (217), Expect = 3e-18
Identities = 55/98 (56%), Positives = 62/98 (63%), Gaps = 1/98 (1%)
Frame = +2
Query: 35 SDIVTSNFIEKIGMVKDSKKFKAVIVRIDSVGGDFHASQSTWEAIRSLASKKPVIASMSD 94
S I+ FIEKI V++SKKFKA I+RIDS GGD AS W IR LA+ KPVIASMSD
Sbjct: 53 SGIIAEKFIEKIRTVRESKKFKAAIIRIDSPGGDALASDLMWREIRLLAASKPVIASMSD 232
Query: 95 AATS-AGYYMAMGAGAIVAENLTLTGSIRGTVSQNFNL 131
+ G IVAE LTLTGSI G V+ FNL
Sbjct: 233 VGSKLEGTTWQWELMFIVAEILTLTGSI-GVVTGKFNL 343
>CO978618
Length = 801
Score = 85.1 bits (209), Expect = 2e-17
Identities = 60/129 (46%), Positives = 71/129 (54%), Gaps = 34/129 (26%)
Frame = -3
Query: 92 MSDAATSAGYYMAMGAGAIVAENLTLTGSIRGTVSQNFNL---------DNELPSFIPY- 141
MSD A S GYYMAMGAG IVAE+LTLTGSI G V+ FNL + E+ S Y
Sbjct: 799 MSDVAASGGYYMAMGAGVIVAESLTLTGSI-GVVTGKFNLGKLYEKIGFNKEIISRGRYA 623
Query: 142 ------------DDAKVL------------DNVVRLRLMTLGKLDKMKKVAHGRIWTGKD 177
D+A++ D R MT+ DKM++VA GR+WTGKD
Sbjct: 622 ELRAAEQRSLRPDEAELFSKSAQHAYKQFRDKAALSRSMTV---DKMEEVAQGRVWTGKD 452
Query: 178 AASHGLVDA 186
AASHGLVDA
Sbjct: 451 AASHGLVDA 425
>TC222979 similar to UP|Q8H0V0 (Q8H0V0) Isp4 like protein, partial (30%)
Length = 661
Score = 64.3 bits (155), Expect = 4e-11
Identities = 28/39 (71%), Positives = 32/39 (81%)
Frame = +2
Query: 188 MPVISYGFAGMPPATPTNFARWPFTRMIFNYFVFRFHKR 226
+PVI+Y FAGMPPATP N A W T MIFNYFVFR++KR
Sbjct: 437 IPVITYRFAGMPPATPANIASWLVTGMIFNYFVFRYNKR 553
>TC213087 similar to PIR|E69990|E69990 proteinase IV homolog yteI - Bacillus
subtilis {Bacillus subtilis;} , partial (53%)
Length = 761
Score = 51.6 bits (122), Expect = 3e-07
Identities = 33/120 (27%), Positives = 60/120 (49%), Gaps = 12/120 (10%)
Frame = +2
Query: 23 QIAIIRASGTMDSDIVTSN-----------FIEKIGMVKDSKKFKAVIVRIDSVGGDFHA 71
+IA++ +GT+ + S+ F++++ K+ K V++R++S G +
Sbjct: 266 KIAVLEINGTIQDNGGASSLLGGEGYDHRAFLKELDKAKEDASEKGVLLRVNSPAGGVYE 445
Query: 72 SQSTWEAIRSLA-SKKPVIASMSDAATSAGYYMAMGAGAIVAENLTLTGSIRGTVSQNFN 130
S + + + +KKP+ SM S GYY++ A I A TLTGS+ G + Q+ N
Sbjct: 446 SAEIHKKLEEVKKAKKPIYVSMGSMVASGGYYVSTPAKKIFASPETLTGSL-GVIMQSLN 622
>TC223443 similar to PIR|F96767|F96767 proteinase IV F2P9.14 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(22%)
Length = 463
Score = 48.5 bits (114), Expect = 2e-06
Identities = 42/112 (37%), Positives = 52/112 (45%), Gaps = 35/112 (31%)
Frame = +3
Query: 110 IVAENLTLTGSIRGTVSQNFNLDN-----------------------ELPSFIPYDDAKV 146
IVAE+LTLTGSI G V+ FNL E F P D+A++
Sbjct: 9 IVAESLTLTGSI-GVVTGKFNLGKLYEKIGFNKEIISRGRYAELLAAEQRPFRP-DEAEL 182
Query: 147 L------------DNVVRLRLMTLGKLDKMKKVAHGRIWTGKDAASHGLVDA 186
D R MT+ +KM++ A GR+WTGKDAA GLVDA
Sbjct: 183 FAKSAQHAYKQFRDKAASSRSMTV---EKMEEFAQGRVWTGKDAALRGLVDA 329
>TC222041 similar to UP|Q8LBQ6 (Q8LBQ6) Isp4-like protein, partial (38%)
Length = 1012
Score = 45.1 bits (105), Expect = 3e-05
Identities = 18/39 (46%), Positives = 25/39 (63%)
Frame = +2
Query: 188 MPVISYGFAGMPPATPTNFARWPFTRMIFNYFVFRFHKR 226
+PV+ MPPATP N+ W F IFN+F+FR+ K+
Sbjct: 542 LPVLLGATGMMPPATPLNYNAWIFVGTIFNFFIFRYRKK 658
>BG839504 similar to GP|20466754|gb Isp4-like protein {Arabidopsis thaliana},
partial (15%)
Length = 499
Score = 40.8 bits (94), Expect = 5e-04
Identities = 15/29 (51%), Positives = 20/29 (68%)
Frame = +3
Query: 198 MPPATPTNFARWPFTRMIFNYFVFRFHKR 226
MPPATP N+ W F IFN+F+FR+ +
Sbjct: 75 MPPATPLNYNAWIFVGTIFNFFIFRYRXK 161
>TC213192 similar to PIR|F96767|F96767 proteinase IV F2P9.14 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(21%)
Length = 620
Score = 40.0 bits (92), Expect = 9e-04
Identities = 18/26 (69%), Positives = 22/26 (84%)
Frame = +3
Query: 161 LDKMKKVAHGRIWTGKDAASHGLVDA 186
+DKM++VA GR+ T KD ASHGLVDA
Sbjct: 96 VDKMEEVAQGRVRTRKDTASHGLVDA 173
>BM085219 weakly similar to GP|9758213|dbj sexual differentiation process
protein ISP4-like {Arabidopsis thaliana}, partial (17%)
Length = 426
Score = 37.4 bits (85), Expect = 0.006
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = -2
Query: 188 MPVISYGFAGMPPATPTNFARWPFTRMIFNYFVF 221
MP+I G G+PPA N+ W F + FN++V+
Sbjct: 245 MPLIIAGGGGIPPARSVNYITWGFVGIFFNFYVY 144
>AW310775 weakly similar to PIR|T08925|T08 isp4 protein homolog T15N24.40 -
Arabidopsis thaliana, partial (14%)
Length = 466
Score = 36.6 bits (83), Expect = 0.010
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -1
Query: 188 MPVISYGFAGMPPATPTNFARWPFTRMIFNYFVFRFHK 225
MP+I G G+PPA N+ W ++FN++V+ K
Sbjct: 439 MPLIIAGSGGIPPARSVNYITWGMVGILFNFYVYNKFK 326
>CO978558
Length = 721
Score = 32.3 bits (72), Expect = 0.18
Identities = 13/38 (34%), Positives = 19/38 (49%)
Frame = -2
Query: 188 MPVISYGFAGMPPATPTNFARWPFTRMIFNYFVFRFHK 225
MPV+ + MPPAT NF W + Y +R+ +
Sbjct: 411 MPVLLGATSMMPPATAVNFTSWILVGFLSGYIAYRYRQ 298
>BQ080748 weakly similar to PIR|T08925|T08 isp4 protein homolog T15N24.40 -
Arabidopsis thaliana, partial (17%)
Length = 421
Score = 32.3 bits (72), Expect = 0.18
Identities = 12/37 (32%), Positives = 20/37 (53%)
Frame = +3
Query: 189 PVISYGFAGMPPATPTNFARWPFTRMIFNYFVFRFHK 225
P+I G + +PP N+ W + FN++V+R K
Sbjct: 204 PIIIAGASNIPPFRSVNYITWGIVGIFFNFYVYRKFK 314
>TC213712 similar to UP|Q9LWW1 (Q9LWW1) ESTs D25093(R3139) (Glutathione
transporter), partial (14%)
Length = 393
Score = 31.6 bits (70), Expect = 0.31
Identities = 15/42 (35%), Positives = 22/42 (51%)
Frame = +2
Query: 182 GLVDAGMPVISYGFAGMPPATPTNFARWPFTRMIFNYFVFRF 223
GL++ MPV+ MPPAT N+ W + + VFR+
Sbjct: 8 GLIN--MPVLIGATGMMPPATAVNYTSWIIAGFLSGFVVFRY 127
>CK605780
Length = 491
Score = 30.0 bits (66), Expect = 0.89
Identities = 14/31 (45%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
Frame = -3
Query: 193 YGFAG-MPPATPTNFARWPFTRMIFNYFVFR 222
+G AG +PPAT +W +IFNYF+ R
Sbjct: 462 FGAAGQIPPATCWYLGQWVIVGLIFNYFIRR 370
>TC217829 similar to UP|Q945L7 (Q945L7) AT4g35240/F23E12_200, partial (68%)
Length = 1872
Score = 30.0 bits (66), Expect = 0.89
Identities = 17/42 (40%), Positives = 25/42 (59%)
Frame = +1
Query: 44 EKIGMVKDSKKFKAVIVRIDSVGGDFHASQSTWEAIRSLASK 85
EK+ ++ D K K + R+D G DFH ST IR+L++K
Sbjct: 445 EKMRVMHDRKCHK--LKRLDDRGADFHKVDSTRTLIRNLSTK 564
>TC226087 similar to UP|Q9ZPI6 (Q9ZPI6) AIM1 protein, partial (55%)
Length = 1223
Score = 29.3 bits (64), Expect = 1.5
Identities = 18/58 (31%), Positives = 30/58 (51%), Gaps = 2/58 (3%)
Frame = +3
Query: 57 AVIVRIDSVGGDFHASQSTWEAIRSLA--SKKPVIASMSDAATSAGYYMAMGAGAIVA 112
+V+ ++ G H + E + + SKKPV+A+++ A G +AMG A VA
Sbjct: 42 SVMQKVHRTGDSSHLPDVSVELVVNSIEDSKKPVVAAVAGLALGGGLELAMGCHARVA 215
>TC221865
Length = 1045
Score = 28.9 bits (63), Expect = 2.0
Identities = 16/43 (37%), Positives = 24/43 (55%), Gaps = 3/43 (6%)
Frame = +1
Query: 54 KFKAVIVRIDSVGGDFHASQSTW---EAIRSLASKKPVIASMS 93
K+ A++V +G DFH QS R ++SKKP++ S S
Sbjct: 607 KWYALLVE*CPLGSDFHTQQSVRCR*MGNRDMSSKKPIVVSTS 735
>BI786148 weakly similar to GP|16769440|gb LD31383p {Drosophila
melanogaster}, partial (1%)
Length = 426
Score = 26.9 bits (58), Expect = 7.5
Identities = 11/23 (47%), Positives = 13/23 (55%)
Frame = -3
Query: 192 SYGFAGMPPATPTNFARWPFTRM 214
SY FA P P +F +W TRM
Sbjct: 232 SYSFAESPNIWPKHFQQWEITRM 164
>TC223162
Length = 782
Score = 26.9 bits (58), Expect = 7.5
Identities = 13/38 (34%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Frame = -3
Query: 11 RKWTVGISEAKEQ--IAIIRASGTMDSDIVTSNFIEKI 46
RKW + + E++ +RASGT+ S+ + S F +I
Sbjct: 567 RKWPLILLESQSNWLSRCLRASGTLSSEAIASTFFTRI 454
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.134 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,308,866
Number of Sequences: 63676
Number of extensions: 85650
Number of successful extensions: 427
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 423
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 426
length of query: 226
length of database: 12,639,632
effective HSP length: 94
effective length of query: 132
effective length of database: 6,654,088
effective search space: 878339616
effective search space used: 878339616
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC144729.7