BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144728.11 + phase: 0 /pseudo
(438 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI974689 42 5e-04
BM527030 42 7e-04
CA851073 37 0.013
TC233587 36 0.030
CF921567 36 0.030
AW830836 35 0.051
TC213899 35 0.051
TC213668 32 0.065
BQ453949 35 0.087
BE610670 35 0.087
TC223713 34 0.15
TC222789 33 0.19
AW595917 33 0.25
CD393541 32 0.43
TC222557 32 0.56
TC211538 30 1.6
CA820304 30 1.6
CD394161 30 1.6
TC222628 30 2.1
TC231904 similar to GB|AAL25568.1|16648753|AY058152 AT5g16150/T2... 30 2.1
>BI974689
Length = 409
Score = 42.0 bits (97), Expect = 5e-04
Identities = 22/55 (40%), Positives = 31/55 (56%), Gaps = 1/55 (1%)
Frame = -1
Query: 302 ALRSPRKLNT*FGVCVGGCLPTRVLLQDKGVSCPTNCASCSHEYEDLN-HLMFEC 355
AL+ P K+ CLP+RV L KGV CP+ CA C + E L+ ++ F+C
Sbjct: 373 ALKVPPKVKHLIRRIQRDCLPSRVRLHAKGVQCPSTCAFCHNNLESLDVNM*FQC 209
>BM527030
Length = 421
Score = 41.6 bits (96), Expect = 7e-04
Identities = 20/51 (39%), Positives = 27/51 (52%), Gaps = 3/51 (5%)
Frame = +3
Query: 321 LPTRVLLQDKGVSCPTN---CASCSHEYEDLNHLMFECPFSIQVWMSAGMW 368
LPTR LQ + + C CS + ED +HL+F C FS Q+W + W
Sbjct: 261 LPTRKNLQCRNILLEEQHQLCPFCSGKEEDSSHLIFTCSFSYQIWQNCYKW 413
>CA851073
Length = 633
Score = 37.4 bits (85), Expect = 0.013
Identities = 18/49 (36%), Positives = 25/49 (50%), Gaps = 1/49 (2%)
Frame = +1
Query: 321 LPTRVLLQDKGVSCPTN-CASCSHEYEDLNHLMFECPFSIQVWMSAGMW 368
LPTR L + VS N C C +E E+ +HL F C + +W + W
Sbjct: 202 LPTRHNLLRRNVSIQENECPLCGYEQEEADHLFFNCKMTRGLWWESMRW 348
>TC233587
Length = 703
Score = 36.2 bits (82), Expect = 0.030
Identities = 19/52 (36%), Positives = 25/52 (47%), Gaps = 3/52 (5%)
Frame = +2
Query: 321 LPTRVLLQDKGVSCPTN---CASCSHEYEDLNHLMFECPFSIQVWMSAGMWY 369
+PT+V LQ + + CA C EDL+ L F CP S +W WY
Sbjct: 68 IPTKVNLQKRQIQLHGQYLLCAFCKEVPEDLSRLFFACPISHNIWK---RWY 214
>CF921567
Length = 686
Score = 36.2 bits (82), Expect = 0.030
Identities = 19/49 (38%), Positives = 25/49 (50%), Gaps = 1/49 (2%)
Frame = -1
Query: 321 LPTRVLLQDKGVSCPT-NCASCSHEYEDLNHLMFECPFSIQVWMSAGMW 368
LPTR L+ + VS T +C C E E +NHL F C + +W W
Sbjct: 530 LPTRENLKRRQVSLHTYSCPLCDLEEESVNHLFFNCSKTRSLWWEPLRW 384
>AW830836
Length = 294
Score = 35.4 bits (80), Expect = 0.051
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = -2
Query: 337 NCASCSHEYEDLNHLMFECPFSIQVW 362
+CA C E E + HL+F CPF+ +W
Sbjct: 86 SCALCHGEEETIQHLLFTCPFASTIW 9
>TC213899
Length = 497
Score = 35.4 bits (80), Expect = 0.051
Identities = 18/49 (36%), Positives = 25/49 (50%), Gaps = 1/49 (2%)
Frame = +3
Query: 321 LPTRVLLQDKGVSC-PTNCASCSHEYEDLNHLMFECPFSIQVWMSAGMW 368
LPTR+ L + + ++C CS E HL F+C IQVW + W
Sbjct: 132 LPTRINL*KRQIEMVESSCPFCSLVEESAGHLFFQCSKIIQVWWESLSW 278
>TC213668
Length = 365
Score = 31.6 bits (70), Expect(2) = 0.065
Identities = 16/43 (37%), Positives = 22/43 (50%)
Frame = +2
Query: 322 PTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVWMS 364
PT LQ++G C + + LNH+ +C FS QVW S
Sbjct: 2 PTCEALQNRG-GVFLERPGCEFDLDTLNHIFVQCQFSQQVWNS 127
Score = 22.3 bits (46), Expect(2) = 0.065
Identities = 10/17 (58%), Positives = 12/17 (69%)
Frame = +1
Query: 401 LLFVGVYGSIEISKFGK 417
+L VGVYG IEI F +
Sbjct: 229 MLHVGVYGCIEIL*FSR 279
>BQ453949
Length = 450
Score = 34.7 bits (78), Expect = 0.087
Identities = 15/43 (34%), Positives = 25/43 (57%), Gaps = 1/43 (2%)
Frame = +3
Query: 321 LPTRVLLQDKGVSCPT-NCASCSHEYEDLNHLMFECPFSIQVW 362
+PT+V L+ + V P+ NC C E E + H+M+ C + +W
Sbjct: 204 IPTKVNLRRRHVKLPSSNCPLCDEEEETVGHVMYSCIKTRHLW 332
>BE610670
Length = 368
Score = 34.7 bits (78), Expect = 0.087
Identities = 16/46 (34%), Positives = 25/46 (53%), Gaps = 1/46 (2%)
Frame = -2
Query: 321 LPTRVLLQDKGVSCPT-NCASCSHEYEDLNHLMFECPFSIQVWMSA 365
LPT+ L+ + V P+ C C E E++ H+MF C + +W A
Sbjct: 139 LPTKANLRRRQVELPSYRCPLCDLEEENVGHIMFSCTRTRSLWWEA 2
>TC223713
Length = 420
Score = 33.9 bits (76), Expect = 0.15
Identities = 15/49 (30%), Positives = 22/49 (44%), Gaps = 1/49 (2%)
Frame = +1
Query: 321 LPTRVLLQDKGVSCPT-NCASCSHEYEDLNHLMFECPFSIQVWMSAGMW 368
LPT+ L + + +C CS+ E+ HL F C I +W W
Sbjct: 172 LPTKANLHRRQIEVTNRSCPFCSNMEEEAGHLFFHCSKIIPIWWETSSW 318
>TC222789
Length = 954
Score = 33.5 bits (75), Expect = 0.19
Identities = 16/49 (32%), Positives = 23/49 (46%), Gaps = 1/49 (2%)
Frame = +2
Query: 321 LPTRVLLQDKGVSC-PTNCASCSHEYEDLNHLMFECPFSIQVWMSAGMW 368
LPT+V L + + +C C + ED HL F C I +W + W
Sbjct: 425 LPTKVNLHRRQIQVMDRSCPFCRNVEEDAGHLFFHCSKIIPIWWESLSW 571
>AW595917
Length = 404
Score = 33.1 bits (74), Expect = 0.25
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +1
Query: 321 LPTRVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVWMSAGMW 368
LPT+ L+ + + C C++ ED HL F C ++ W + W
Sbjct: 70 LPTKANLRRQMEISDSLCPLCNNSKEDATHLFFNCSKTLPFWWESLSW 213
>CD393541
Length = 557
Score = 32.3 bits (72), Expect = 0.43
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = -3
Query: 336 TNCASCSHEYEDLNHLMFECPFSIQVWMSAGMW 368
T+C C +E ED HL F C +W + W
Sbjct: 555 TSCPLCRNEVEDAGHLFFHCRKVTPLWWESMSW 457
>TC222557
Length = 1002
Score = 32.0 bits (71), Expect = 0.56
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +1
Query: 338 CASCSHEYEDLNHLMFECPFSIQVWMSAGMW 368
C C+ + E+ +HL F CP + +W + W
Sbjct: 556 CPFCNRQEEEASHLFFNCPRILPLWWESLAW 648
>TC211538
Length = 987
Score = 30.4 bits (67), Expect = 1.6
Identities = 12/33 (36%), Positives = 15/33 (45%)
Frame = +2
Query: 336 TNCASCSHEYEDLNHLMFECPFSIQVWMSAGMW 368
+ C C E E HL F+C I VW + W
Sbjct: 563 SGCPFCRAEEESAGHLFFQCSKIIPVWWESLSW 661
>CA820304
Length = 407
Score = 30.4 bits (67), Expect = 1.6
Identities = 14/39 (35%), Positives = 22/39 (55%)
Frame = +3
Query: 324 RVLLQDKGVSCPTNCASCSHEYEDLNHLMFECPFSIQVW 362
+++ +KG CP CS+E + +L F C S+QVW
Sbjct: 60 KIVFLNKGFLCPL----CSNEPKSNAYLFFSCRKSLQVW 164
>CD394161
Length = 531
Score = 30.4 bits (67), Expect = 1.6
Identities = 11/32 (34%), Positives = 15/32 (46%)
Frame = -1
Query: 337 NCASCSHEYEDLNHLMFECPFSIQVWMSAGMW 368
NC C ED+ HL F C + +W + W
Sbjct: 522 NCPLCGTALEDVAHLFFNCNLTKGLWWESMRW 427
>TC222628
Length = 688
Score = 30.0 bits (66), Expect = 2.1
Identities = 15/58 (25%), Positives = 26/58 (43%), Gaps = 1/58 (1%)
Frame = +1
Query: 336 TNCASCSHEYEDLNHLMFECPFSIQVWMSAGMWY-MCKMRP*IRTLLSTQFSIYSKTR 392
T+C C+ E++ HL C Q+W W + P + L QF ++++ R
Sbjct: 460 TSCYFCNQSMENMKHLFLSCSKISQIWYHVCRWMGVDVFLPQDISQLYNQFGLFNRGR 633
>TC231904 similar to GB|AAL25568.1|16648753|AY058152 AT5g16150/T21H19_70
{Arabidopsis thaliana;} , partial (17%)
Length = 903
Score = 30.0 bits (66), Expect = 2.1
Identities = 15/49 (30%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
Frame = +1
Query: 321 LPTRVLLQDKGVSCPTN-CASCSHEYEDLNHLMFECPFSIQVWMSAGMW 368
LPT+ L+ + + + C CS + E +HL FEC +W + W
Sbjct: 304 LPTKSNLRRRQIDISDSLCPFCSIKDETASHLFFECSKIQHLWWESQSW 450
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.359 0.163 0.562
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,106,591
Number of Sequences: 63676
Number of extensions: 363238
Number of successful extensions: 3864
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 2900
Number of HSP's successfully gapped in prelim test: 128
Number of HSP's that attempted gapping in prelim test: 921
Number of HSP's gapped (non-prelim): 3094
length of query: 438
length of database: 12,639,632
effective HSP length: 100
effective length of query: 338
effective length of database: 6,272,032
effective search space: 2119946816
effective search space used: 2119946816
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC144728.11