BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144727.12 - phase: 0
(160 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC219569 289 3e-79
AW569307 215 6e-57
BE352758 140 3e-34
AW733733 weakly similar to GP|21536663|gb plasma membrane associ... 121 1e-28
TC229520 UP|Q39872 (Q39872) PGmPM3, complete 117 2e-27
BG654210 29 1.1
TC211478 29 1.1
TC220069 similar to UP|Q9SSY8 (Q9SSY8) Hsr203J homolog, partial ... 28 1.4
TC210772 similar to UP|O80358 (O80358) AtMMH-1 protein (F6D8.28 ... 28 1.4
TC204043 similar to UP|Q9XQB1 (Q9XQB1) LHCII type III chlorophyl... 28 2.4
BM270612 27 3.2
TC212711 weakly similar to GB|AAR28015.1|39545904|AY463613 TAF12... 27 4.1
TC231323 UP|Q8S4Q4 (Q8S4Q4) Nodulin 6l, complete 27 4.1
TC228474 26 9.2
TC224997 homologue to UP|Q7Y199 (Q7Y199) 40S subunit ribosomal p... 26 9.2
TC204008 homologue to UP|Q9XQB1 (Q9XQB1) LHCII type III chloroph... 26 9.2
BI967508 26 9.2
>TC219569
Length = 790
Score = 289 bits (740), Expect = 3e-79
Identities = 141/160 (88%), Positives = 151/160 (94%)
Frame = +1
Query: 1 MANEQMKPIATLLLALNFCMYVIVISIGGWAMNRAIDHGFEIGPGFDLPAHFSPIYFPMG 60
MA +QMKPIATLLL LNFCMYVIV+ IGGWAMNRAIDHGF IGPGFDLPAHFSPIYFPMG
Sbjct: 85 MATDQMKPIATLLLGLNFCMYVIVLGIGGWAMNRAIDHGFVIGPGFDLPAHFSPIYFPMG 264
Query: 61 NAATGFFVTFALIAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLAMGFGCK 120
NAATGFFVTFALIAGV G+GS+ISG+NH+RSWTSESLPSAASVA+IAWALTVLAMGF CK
Sbjct: 265 NAATGFFVTFALIAGVAGVGSIISGVNHVRSWTSESLPSAASVASIAWALTVLAMGFACK 444
Query: 121 EIQLNIRNSRLKTMEAFLIILTATQLFYIAAIHGAAAIRR 160
EIQL RN+RLKTMEAF+IIL+ATQLFYIAAIHGAAA RR
Sbjct: 445 EIQLTGRNARLKTMEAFMIILSATQLFYIAAIHGAAAYRR 564
>AW569307
Length = 410
Score = 215 bits (548), Expect = 6e-57
Identities = 103/131 (78%), Positives = 115/131 (87%)
Frame = +2
Query: 1 MANEQMKPIATLLLALNFCMYVIVISIGGWAMNRAIDHGFEIGPGFDLPAHFSPIYFPMG 60
MA EQMKPIATLLL LNFCMY IV+ IGGWAMNRAIDHGF IGP LPAHFSP++FPMG
Sbjct: 17 MAKEQMKPIATLLLGLNFCMYAIVLGIGGWAMNRAIDHGFIIGPELKLPAHFSPLFFPMG 196
Query: 61 NAATGFFVTFALIAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLAMGFGCK 120
NA+TGFFVTFAL+AGVVG S ISG+NHI+SWT+ESLPSAASVA +AW LT+LAMGF K
Sbjct: 197 NASTGFFVTFALLAGVVGAASAISGINHIQSWTAESLPSAASVATMAWTLTLLAMGFAWK 376
Query: 121 EIQLNIRNSRL 131
EI+L IRN+RL
Sbjct: 377 EIELRIRNARL 409
>BE352758
Length = 394
Score = 140 bits (353), Expect = 3e-34
Identities = 68/82 (82%), Positives = 73/82 (88%), Gaps = 1/82 (1%)
Frame = +1
Query: 1 MANEQMKPIATLLLALNFCMYVIVISIGGWAMNRAIDHGFEIGPGFDLPAHFSPIYFPMG 60
MAN+QMKPIATLLL LNFCMYVIV+ IGGWAMNRAIDHGF IGPGFDLPAHFSPIYFPMG
Sbjct: 103 MANDQMKPIATLLLGLNFCMYVIVLGIGGWAMNRAIDHGFVIGPGFDLPAHFSPIYFPMG 282
Query: 61 NAATGFFVTFALIA-GVVGIGS 81
AATGFF TFAL A +G+G+
Sbjct: 283 XAATGFFGTFALNAWKCLGVGT 348
>AW733733 weakly similar to GP|21536663|gb plasma membrane associated
protein-like {Arabidopsis thaliana}, partial (71%)
Length = 435
Score = 121 bits (304), Expect = 1e-28
Identities = 58/118 (49%), Positives = 77/118 (65%)
Frame = +2
Query: 7 KPIATLLLALNFCMYVIVISIGGWAMNRAIDHGFEIGPGFDLPAHFSPIYFPMGNAATGF 66
K +A++LLALN +Y IV+ I WA+N I E +PA PIYFPMGN TGF
Sbjct: 68 KSVASILLALNLVLYFIVLVIASWAVNHGIQRSGETASVLSIPARIFPIYFPMGNMTTGF 247
Query: 67 FVTFALIAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLAMGFGCKEIQL 124
FV +L+AGVVG + ++GL +I W + +L +AA + WALT+LAMGF CKEI+L
Sbjct: 248 FVILSLVAGVVGFTTSLTGLQNIFQWNAPNLHAAAMSSLTTWALTLLAMGFACKEIEL 421
>TC229520 UP|Q39872 (Q39872) PGmPM3, complete
Length = 1018
Score = 117 bits (293), Expect = 2e-27
Identities = 66/146 (45%), Positives = 88/146 (60%), Gaps = 1/146 (0%)
Frame = +3
Query: 9 IATLLLALNFCMYVIVISIGGWAMNRAIDHGFEIGPGFDLPAHFSPIYFPMGNAATGFFV 68
+A LL LN MY IV+ W +NR I+ G P F GN AT FF+
Sbjct: 174 VAAPLLFLNLIMYFIVLGFASWCLNRFIN-GQTYHPSFG------------GNGATMFFL 314
Query: 69 TFALIAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLAMGFGCKEIQL-NIR 127
TF+++A V+GI S + G NH+R+W S+SL SA + + +AWA+T LA G CK+I L R
Sbjct: 315 TFSILAAVLGIVSKLLGGNHMRTWRSDSLASAGATSMVAWAVTALAFGLACKQIHLGGHR 494
Query: 128 NSRLKTMEAFLIILTATQLFYIAAIH 153
RL+ +EAF+IILT TQL Y+ IH
Sbjct: 495 GWRLRVVEAFIIILTFTQLLYLILIH 572
>BG654210
Length = 374
Score = 28.9 bits (63), Expect = 1.1
Identities = 17/54 (31%), Positives = 34/54 (62%)
Frame = -1
Query: 68 VTFALIAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLAMGFGCKE 121
+ F L A +V IGS SG I + +S ++ +++ A+A+ + +++GFGC++
Sbjct: 212 ILFNLSAYMVSIGS--SGTLTIGAESSSAI*TSSCPLALAF*VEAVSLGFGCED 57
>TC211478
Length = 483
Score = 28.9 bits (63), Expect = 1.1
Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = -1
Query: 94 SESLPSAASVAAIAWALTVLAMGFGCKEIQLNIR-NSRLKTMEAFLIILTA 143
SES+ + + + W L+V+ G G +Q+N+R S L T+E +L A
Sbjct: 360 SESIERSWRLC*VTWELSVVDEGEGVSVLQINLRARSILATLETRASVLNA 208
>TC220069 similar to UP|Q9SSY8 (Q9SSY8) Hsr203J homolog, partial (88%)
Length = 1071
Score = 28.5 bits (62), Expect = 1.4
Identities = 17/49 (34%), Positives = 25/49 (50%), Gaps = 4/49 (8%)
Frame = +2
Query: 78 GIGSLISGLNHIRSWTSESLP----SAASVAAIAWALTVLAMGFGCKEI 122
G+G + LN SW + LP S AI+W +T+ GF C++I
Sbjct: 32 GLGPVRQKLN---SWLNRFLPMKNLSMELPLAISWLVTISVSGFTCRKI 169
>TC210772 similar to UP|O80358 (O80358) AtMMH-1 protein (F6D8.28 protein),
partial (72%)
Length = 986
Score = 28.5 bits (62), Expect = 1.4
Identities = 26/94 (27%), Positives = 37/94 (38%), Gaps = 11/94 (11%)
Frame = -3
Query: 49 PAHFSPIYFPMGNAATGFF--------VTFALIAGVVGIGSLISGLNHIRSWTSESLPSA 100
PA I+FP A GFF V + L++ I ++ L H+ + SL
Sbjct: 792 PAVMKSIFFPSTKALPGFFSRE*NIQLVGY*LLSAPTSIAFSMTSLMHLLKFEQLSLDRL 613
Query: 101 ASVA---AIAWALTVLAMGFGCKEIQLNIRNSRL 131
+ +AW T A F EI+L N L
Sbjct: 612 EATCRG*ILAWYNTSSATQFPIPEIKL*SSNKAL 511
>TC204043 similar to UP|Q9XQB1 (Q9XQB1) LHCII type III chlorophyll a/b
binding protein, partial (62%)
Length = 707
Score = 27.7 bits (60), Expect = 2.4
Identities = 14/29 (48%), Positives = 17/29 (58%)
Frame = -2
Query: 80 GSLISGLNHIRSWTSESLPSAASVAAIAW 108
GSL S LNH S + PSA S+A + W
Sbjct: 337 GSLKSTLNHFSSTSGVMHPSAPSMAHLPW 251
>BM270612
Length = 421
Score = 27.3 bits (59), Expect = 3.2
Identities = 13/32 (40%), Positives = 18/32 (55%)
Frame = -2
Query: 116 GFGCKEIQLNIRNSRLKTMEAFLIILTATQLF 147
GFG + +Q I N K ++A LII+ LF
Sbjct: 303 GFGARVVQCTIGNDARKKLQA*LIIIIGKTLF 208
>TC212711 weakly similar to GB|AAR28015.1|39545904|AY463613 TAF12
{Arabidopsis thaliana;} , partial (19%)
Length = 428
Score = 26.9 bits (58), Expect = 4.1
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = -2
Query: 91 SWTSESLPSAASVAAIAWALTV 112
+W +E P AASVAAI + +TV
Sbjct: 343 AWPAEPFPCAASVAAIDFFITV 278
>TC231323 UP|Q8S4Q4 (Q8S4Q4) Nodulin 6l, complete
Length = 1452
Score = 26.9 bits (58), Expect = 4.1
Identities = 35/116 (30%), Positives = 47/116 (40%), Gaps = 7/116 (6%)
Frame = +3
Query: 36 IDHGFEIGPGFDLPAH--FSPIYFPMGNAATGFFVTFALIAGVVGIGSLISGLNHIRSWT 93
ID G E+ D+ H F+P FV L V L GL + SWT
Sbjct: 423 IDDGIEMDKKHDMQWHKNFTP------------FVGRILRTERVAEQILEQGLPNGSSWT 566
Query: 94 SESLPSA-----ASVAAIAWALTVLAMGFGCKEIQLNIRNSRLKTMEAFLIILTAT 144
+S A SVA AL +A +G EI LN+ ++ E+ +LT T
Sbjct: 567 IDSFTEAYVSKVKSVAKEIHALKSIAAYYGGLEINLNV--TKTDAEESLKEVLTNT 728
>TC228474
Length = 953
Score = 25.8 bits (55), Expect = 9.2
Identities = 13/39 (33%), Positives = 20/39 (50%)
Frame = -3
Query: 10 ATLLLALNFCMYVIVISIGGWAMNRAIDHGFEIGPGFDL 48
++L+ L+FC + I G ++ R I I P FDL
Sbjct: 252 SSLVAELSFCCCCSLNLISGPSLERLITQNSTISPAFDL 136
>TC224997 homologue to UP|Q7Y199 (Q7Y199) 40S subunit ribosomal protein,
partial (27%)
Length = 992
Score = 25.8 bits (55), Expect = 9.2
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +2
Query: 108 WALTVLAMGFGCKEIQLNIRNSRLKTMEAFLIIL 141
W L+V + G K +N+ SRL T + FL IL
Sbjct: 605 WLLSVEILHEGKKTNTVNMIPSRLHTFDVFLFIL 706
>TC204008 homologue to UP|Q9XQB1 (Q9XQB1) LHCII type III chlorophyll a/b
binding protein, complete
Length = 1220
Score = 25.8 bits (55), Expect = 9.2
Identities = 14/29 (48%), Positives = 16/29 (54%)
Frame = -1
Query: 80 GSLISGLNHIRSWTSESLPSAASVAAIAW 108
GSL S LNH S + PSA S+A W
Sbjct: 527 GSLKSTLNHFSSTSGVMHPSAPSMAHRPW 441
>BI967508
Length = 561
Score = 25.8 bits (55), Expect = 9.2
Identities = 8/20 (40%), Positives = 14/20 (70%)
Frame = -3
Query: 11 TLLLALNFCMYVIVISIGGW 30
T++L + FC+ ++ I GGW
Sbjct: 133 TIILRVRFCLDLVFILFGGW 74
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.328 0.140 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,919,494
Number of Sequences: 63676
Number of extensions: 90121
Number of successful extensions: 591
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 589
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 590
length of query: 160
length of database: 12,639,632
effective HSP length: 90
effective length of query: 70
effective length of database: 6,908,792
effective search space: 483615440
effective search space used: 483615440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC144727.12