BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144725.4 + phase: 0
(126 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI425013 76 5e-15
TC216647 similar to UP|WR41_ARATH (Q8H0Y8) Probable WRKY transcr... 73 4e-14
TC217291 similar to UP|WR30_ARATH (Q9FL62) Probable WRKY transcr... 71 1e-13
TC217290 weakly similar to UP|WR46_ARATH (Q9SKD9) Probable WRKY ... 69 4e-13
TC209320 similar to UP|WR41_ARATH (Q8H0Y8) Probable WRKY transcr... 69 6e-13
TC204227 weakly similar to UP|Q9M6E1 (Q9M6E1) DNA-binding protei... 69 7e-13
TC216116 similar to UP|Q9M6E1 (Q9M6E1) DNA-binding protein 3, pa... 66 4e-12
TC232487 similar to UP|WR41_ARATH (Q8H0Y8) Probable WRKY transcr... 65 6e-12
TC204230 similar to UP|Q9M6E0 (Q9M6E0) DNA-binding protein 4 (Fr... 61 2e-10
TC226892 similar to UP|Q6IEN1 (Q6IEN1) WRKY transcription factor... 60 2e-10
TC219386 similar to UP|WR24_ARATH (Q9FFS3) Probable WRKY transcr... 59 4e-10
TC226891 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-... 59 4e-10
TC221170 similar to UP|Q94IB3 (Q94IB3) WRKY DNA-binding protein,... 59 6e-10
TC216813 similar to UP|WR11_ARATH (Q9SV15) Probable WRKY transcr... 59 6e-10
TC216811 similar to UP|WR11_ARATH (Q9SV15) Probable WRKY transcr... 59 6e-10
TC227106 similar to UP|WR23_ARATH (O22900) Probable WRKY transcr... 58 1e-09
TC227101 homologue to UP|WR23_ARATH (O22900) Probable WRKY trans... 58 1e-09
TC211307 homologue to UP|WR57_ARATH (Q9C983) Probable WRKY trans... 58 1e-09
AI966709 weakly similar to PIR|T50861|T508 DNA-binding protein 4... 58 1e-09
TC208493 similar to UP|Q9FXS1 (Q9FXS1) WRKY transcription factor... 58 1e-09
>BI425013
Length = 281
Score = 75.9 bits (185), Expect = 5e-15
Identities = 31/64 (48%), Positives = 40/64 (62%)
Frame = +2
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P EDG+ W KYG K+I G YPRSYYRC C A K+VQ+ + P +F++ Y G H
Sbjct: 44 PHEDGYNWRKYGQKDILGAKYPRSYYRCTFRSTQGCWATKQVQRSDEDPTLFDITYRGNH 223
Query: 62 TCRM 65
TC +
Sbjct: 224 TCSL 235
>TC216647 similar to UP|WR41_ARATH (Q8H0Y8) Probable WRKY transcription
factor 41 (WRKY DNA-binding protein 41), partial (28%)
Length = 1253
Score = 72.8 bits (177), Expect = 4e-14
Identities = 30/62 (48%), Positives = 38/62 (60%)
Frame = +1
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P ED + W KYG K+I G YPRSYYRC C A K+VQ+ + P +F++ Y G H
Sbjct: 145 PHEDSYNWRKYGQKDILGAKYPRSYYRCTFRNTQGCWATKQVQRSDEDPTVFDITYRGKH 324
Query: 62 TC 63
TC
Sbjct: 325 TC 330
>TC217291 similar to UP|WR30_ARATH (Q9FL62) Probable WRKY transcription
factor 30 (WRKY DNA-binding protein 30), partial (21%)
Length = 1108
Score = 70.9 bits (172), Expect = 1e-13
Identities = 29/72 (40%), Positives = 43/72 (59%)
Frame = +1
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
+DG++W KYG K+I G +PR YYRC + + C A K+VQ+ + P I E+ Y G HTC
Sbjct: 220 DDGYSWRKYGQKDILGAKFPRGYYRCTYRNVQGCLATKQVQKSDEDPMICEITYKGRHTC 399
Query: 64 RMSLTIPSLFVP 75
+ + +P
Sbjct: 400 TQASHLNKTVIP 435
>TC217290 weakly similar to UP|WR46_ARATH (Q9SKD9) Probable WRKY
transcription factor 46 (WRKY DNA-binding protein 46),
partial (28%)
Length = 1151
Score = 69.3 bits (168), Expect = 4e-13
Identities = 28/60 (46%), Positives = 39/60 (64%)
Frame = +1
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
+DG++W KYG K+I G +PR YYRC + + C A K+VQ+ + P I E+ Y G HTC
Sbjct: 175 DDGYSWRKYGQKDILGAKFPRGYYRCTYRNVQGCLATKQVQKSDEDPMICEITYKGRHTC 354
>TC209320 similar to UP|WR41_ARATH (Q8H0Y8) Probable WRKY transcription
factor 41 (WRKY DNA-binding protein 41), partial (27%)
Length = 1201
Score = 68.9 bits (167), Expect = 6e-13
Identities = 29/70 (41%), Positives = 40/70 (56%)
Frame = +1
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
+DG++W KYG K+I G +PR YYRC + C A K+VQ+ + P EV Y G HTC
Sbjct: 85 DDGYSWRKYGQKDILGAKFPRGYYRCTQRNVQGCLATKQVQRSDEDPTTIEVTYRGRHTC 264
Query: 64 RMSLTIPSLF 73
+ + F
Sbjct: 265 TQAKHLNKAF 294
>TC204227 weakly similar to UP|Q9M6E1 (Q9M6E1) DNA-binding protein 3, partial
(25%)
Length = 1110
Score = 68.6 bits (166), Expect = 7e-13
Identities = 35/95 (36%), Positives = 52/95 (53%), Gaps = 1/95 (1%)
Frame = +1
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P +DG W KYG KEI +PR+YYRC H C+A K+VQ++ + P +++ Y G H
Sbjct: 397 PIDDGHHWRKYGQKEILNAKFPRNYYRCTHKFDQGCQATKQVQRVQEEPILYKTTYYGLH 576
Query: 62 TCRMSLTIPSLFVPARQPLDIS-IDGIQSTMPTSS 95
TC+ +L P + + P S ++ PT S
Sbjct: 577 TCK-NLANPEIILDPMSPSSSSKFLSFDNSFPTPS 678
>TC216116 similar to UP|Q9M6E1 (Q9M6E1) DNA-binding protein 3, partial (26%)
Length = 1525
Score = 66.2 bits (160), Expect = 4e-12
Identities = 28/69 (40%), Positives = 42/69 (60%)
Frame = +2
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
+D W KYG KEI +PRSY+RC CRA K+VQ++ ++P+ + + Y G HTC
Sbjct: 536 DDNHAWRKYGQKEILNSQFPRSYFRCTRKFEQGCRATKQVQRIQENPDRYNITYIGFHTC 715
Query: 64 RMSLTIPSL 72
+ +L P +
Sbjct: 716 KDTLKAPQV 742
>TC232487 similar to UP|WR41_ARATH (Q8H0Y8) Probable WRKY transcription
factor 41 (WRKY DNA-binding protein 41), partial (18%)
Length = 758
Score = 65.5 bits (158), Expect = 6e-12
Identities = 32/80 (40%), Positives = 46/80 (57%)
Frame = +3
Query: 7 FTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTCRMS 66
++W KYG K+I YPRSYYRC K C A K+VQ+ + IF++ Y G HTC+ +
Sbjct: 3 YSWRKYGQKDILSAKYPRSYYRCTFRKTKGCFATKQVQRSEEDHTIFDITYRGSHTCKRN 182
Query: 67 LTIPSLFVPARQPLDISIDG 86
+ +P + P D + DG
Sbjct: 183DAV----LPPKSP-DYTQDG 227
>TC204230 similar to UP|Q9M6E0 (Q9M6E0) DNA-binding protein 4 (Fragment),
partial (24%)
Length = 717
Score = 60.8 bits (146), Expect = 2e-10
Identities = 29/72 (40%), Positives = 41/72 (56%)
Frame = +3
Query: 8 TWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTCRMSL 67
T KYG KEI +PR+YYRC H C+A K+VQ++ + P +F+ Y G HTC+ S
Sbjct: 33 TXRKYGQKEILNAKFPRNYYRCTHKFDQGCQATKQVQRVQEEPILFKTTYYGHHTCKNSA 212
Query: 68 TIPSLFVPARQP 79
P + + P
Sbjct: 213 N-PDIILDPMSP 245
>TC226892 similar to UP|Q6IEN1 (Q6IEN1) WRKY transcription factor 51, partial
(47%)
Length = 1269
Score = 60.5 bits (145), Expect = 2e-10
Identities = 27/59 (45%), Positives = 36/59 (60%)
Frame = +3
Query: 3 PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P D ++W KYG K I G YPR YY+C+ V+ C A+K V++ D PN+ V Y G H
Sbjct: 783 PVDEYSWRKYGQKPIKGSPYPRGYYKCSSVR--GCPARKHVERAQDDPNMLIVTYEGEH 953
>TC219386 similar to UP|WR24_ARATH (Q9FFS3) Probable WRKY transcription
factor 24 (WRKY DNA-binding protein 24), partial (52%)
Length = 775
Score = 59.3 bits (142), Expect = 4e-10
Identities = 26/58 (44%), Positives = 33/58 (56%)
Frame = +2
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
+DG+ W KYG K + YPRSYYRC H + C KK+VQ+L +I Y G H
Sbjct: 350 DDGYRWRKYGQKAVKNNTYPRSYYRCTH---HTCNVKKQVQRLSKDTSIVVTTYEGIH 514
>TC226891 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-binding
protein {Arabidopsis thaliana;} , partial (41%)
Length = 1187
Score = 59.3 bits (142), Expect = 4e-10
Identities = 28/66 (42%), Positives = 38/66 (57%)
Frame = +2
Query: 3 PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
P D ++W KYG K I G YPR YY+C+ V+ C A+K V++ D P + V Y G H
Sbjct: 680 PPDEYSWRKYGQKPIKGSPYPRGYYKCSTVR--GCPARKHVERAQDDPKMLIVTYEGEHR 853
Query: 63 CRMSLT 68
+ LT
Sbjct: 854 HVLPLT 871
>TC221170 similar to UP|Q94IB3 (Q94IB3) WRKY DNA-binding protein, partial
(14%)
Length = 936
Score = 58.9 bits (141), Expect = 6e-10
Identities = 27/60 (45%), Positives = 36/60 (60%)
Frame = +3
Query: 2 PPEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P EDG+ W KYG K++ G YP SYY+C H C+ KKKV++ +I E+ Y G H
Sbjct: 423 PSEDGYNWRKYGQKQVKGSEYPLSYYKCTHPN---CQVKKKVER-SHEGHITEIIYKGTH 590
>TC216813 similar to UP|WR11_ARATH (Q9SV15) Probable WRKY transcription
factor 11 (WRKY DNA-binding protein 11), partial (46%)
Length = 1173
Score = 58.9 bits (141), Expect = 6e-10
Identities = 26/59 (44%), Positives = 35/59 (59%)
Frame = +2
Query: 3 PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGH 61
P D ++W KYG K I G YPR YY+C+ V+ C A+K V++ D P + V Y G H
Sbjct: 785 PPDEYSWRKYGQKPIKGSPYPRGYYKCSTVR--GCPARKHVERASDDPTMLIVTYEGEH 955
>TC216811 similar to UP|WR11_ARATH (Q9SV15) Probable WRKY transcription
factor 11 (WRKY DNA-binding protein 11), partial (29%)
Length = 686
Score = 58.9 bits (141), Expect = 6e-10
Identities = 28/67 (41%), Positives = 37/67 (54%)
Frame = +1
Query: 3 PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
P D ++W KYG K I G YPR YY+C+ V+ C A+K V++ D P + V Y G H
Sbjct: 166 PPDEYSWRKYGQKPIKGSPYPRGYYKCSSVR--GCPARKHVERAPDDPTMLIVTYEGEHR 339
Query: 63 CRMSLTI 69
M I
Sbjct: 340 HSMQENI 360
>TC227106 similar to UP|WR23_ARATH (O22900) Probable WRKY transcription
factor 23 (WRKY DNA-binding protein 23), partial (32%)
Length = 783
Score = 58.2 bits (139), Expect = 1e-09
Identities = 26/59 (44%), Positives = 34/59 (57%)
Frame = +3
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
EDG+ W KYG K + +PRSYYRC V +C KK+V++ P+I Y G HT
Sbjct: 162 EDGYRWRKYGQKAVKNSPFPRSYYRCTSV---SCNVKKRVERSFSDPSIVVTTYEGQHT 329
>TC227101 homologue to UP|WR23_ARATH (O22900) Probable WRKY transcription
factor 23 (WRKY DNA-binding protein 23), partial (31%)
Length = 599
Score = 58.2 bits (139), Expect = 1e-09
Identities = 26/59 (44%), Positives = 34/59 (57%)
Frame = +1
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
EDG+ W KYG K + +PRSYYRC V +C KK+V++ P+I Y G HT
Sbjct: 115 EDGYRWRKYGQKAVKNSPFPRSYYRCTSV---SCNVKKRVERSFSDPSIVVTTYEGQHT 282
>TC211307 homologue to UP|WR57_ARATH (Q9C983) Probable WRKY transcription
factor 57 (WRKY DNA-binding protein 57), partial (29%)
Length = 761
Score = 57.8 bits (138), Expect = 1e-09
Identities = 27/67 (40%), Positives = 37/67 (54%)
Frame = +2
Query: 4 EDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
EDG+ W KYG K + +PRSYYRC + K C KK+V++ + P I Y G H C
Sbjct: 17 EDGYRWRKYGQKAVKNSPFPRSYYRCTNSK---CTVKKRVERSSEDPTIVITTYEGQH-C 184
Query: 64 RMSLTIP 70
++ P
Sbjct: 185HHTVGFP 205
>AI966709 weakly similar to PIR|T50861|T508 DNA-binding protein 4
[imported] - common tobacco (fragment), partial (25%)
Length = 181
Score = 57.8 bits (138), Expect = 1e-09
Identities = 25/59 (42%), Positives = 34/59 (57%)
Frame = +2
Query: 5 DGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHTC 63
D W KYG K + +PRSY+RC H C AKK+VQ+ ++ N++ Y G HTC
Sbjct: 2 DNHVWRKYGQKGVLNSEFPRSYFRCGHKYDQGCLAKKQVQRDQENSNMYRTTYIGIHTC 178
>TC208493 similar to UP|Q9FXS1 (Q9FXS1) WRKY transcription factor NtEIG-D48,
partial (31%)
Length = 563
Score = 57.8 bits (138), Expect = 1e-09
Identities = 26/64 (40%), Positives = 38/64 (58%)
Frame = +1
Query: 3 PEDGFTWTKYGMKEIPGFIYPRSYYRCNHVKLYACRAKKKVQQLGDHPNIFEVRYNGGHT 62
P D ++W KYG K I G +PR YY+C+ V+ C A+K V++ D P++ V Y G H
Sbjct: 208 PPDDYSWRKYGQKPIKGSPHPRGYYKCSSVR--GCPARKHVERALDDPSMLVVTYEGEHN 381
Query: 63 CRMS 66
+S
Sbjct: 382 HTLS 393
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.139 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,467,081
Number of Sequences: 63676
Number of extensions: 91607
Number of successful extensions: 519
Number of sequences better than 10.0: 138
Number of HSP's better than 10.0 without gapping: 503
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 506
length of query: 126
length of database: 12,639,632
effective HSP length: 86
effective length of query: 40
effective length of database: 7,163,496
effective search space: 286539840
effective search space used: 286539840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)
Medicago: description of AC144725.4