BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144618.2 + phase: 0
(106 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC208446 similar to UP|MNN4_YEAST (P36044) MNN4 protein, partial... 59 4e-10
TC208445 similar to UP|Q876D1 (Q876D1) MNN4 (Fragment), partial ... 58 6e-10
TC210862 weakly similar to UP|Q9EST5 (Q9EST5) Proliferation rela... 54 9e-09
TC215036 similar to PIR|T06397|T06397 isoprenylated protein FP6 ... 47 1e-06
BQ298167 weakly similar to GP|5732079|gb|A T32N4.11 gene product... 47 2e-06
TC207614 45 7e-06
TC217038 43 3e-05
TC228547 40 2e-04
TC228803 PIR|T06397|T06397 isoprenylated protein FP6 - soybean (... 40 2e-04
CF922268 33 0.028
TC207738 similar to UP|Q9FFE6 (Q9FFE6) AMP-binding protein (Aden... 27 1.5
TC226625 similar to UP|THIF_PEA (P29450) Thioredoxin F-type, chl... 26 2.6
TC216284 similar to GB|AAP68253.1|31711794|BT008814 At3g07950 {A... 25 4.4
CO985244 25 4.4
AI794703 25 5.8
TC218290 similar to UP|Q93VG8 (Q93VG8) AT4g17486/AT4g17486, part... 25 5.8
TC224304 UP|Q7PWX9 (Q7PWX9) EbiP4168 (Fragment), partial (25%) 25 7.6
BU760700 24 9.9
TC227271 similar to GB|AAG24276.1|10880493|AF195889 fasciclin-li... 24 9.9
TC231687 weakly similar to UP|Q84QE3 (Q84QE3) Avr9/Cf-9 rapidly ... 24 9.9
>TC208446 similar to UP|MNN4_YEAST (P36044) MNN4 protein, partial (3%)
Length = 565
Score = 58.9 bits (141), Expect = 4e-10
Identities = 30/77 (38%), Positives = 48/77 (61%)
Frame = +2
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVS 60
M+ K RNKA+ IA V GV +++GD+ D++ V+G +D V LA+ L+KKF +++
Sbjct: 62 MDCDKCRNKALKIAAEVRGVTTVSLEGDDNDRVAVSGVNVDMVCLANQLKKKFSSVTILT 241
Query: 61 IEAVGKTEEKKDKEKAE 77
+ + K EE K K+ E
Sbjct: 242 VVDLVKEEEAKKKKDEE 292
>TC208445 similar to UP|Q876D1 (Q876D1) MNN4 (Fragment), partial (9%)
Length = 1040
Score = 58.2 bits (139), Expect = 6e-10
Identities = 33/84 (39%), Positives = 50/84 (59%), Gaps = 6/84 (7%)
Frame = +1
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFG------ 54
M+ K RNKA+ IA V GV +++GD+ D++ V+G +D V LA L+KKF
Sbjct: 85 MDCDKCRNKALKIAAEVRGVTTVSLEGDDNDRVAVSGVNVDMVCLAYQLKKKFSSVTILT 264
Query: 55 YADLVSIEAVGKTEEKKDKEKAEA 78
DLV E K +++++K+K EA
Sbjct: 265 VVDLVKEEEAKKKKDEEEKKKEEA 336
>TC210862 weakly similar to UP|Q9EST5 (Q9EST5) Proliferation related acidic
leucine rich protein PAL31 (Acidic nuclear
phosphoprotein 32 family, member B), partial (7%)
Length = 855
Score = 54.3 bits (129), Expect = 9e-09
Identities = 35/101 (34%), Positives = 54/101 (52%), Gaps = 12/101 (11%)
Frame = +1
Query: 18 SGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTE--------E 69
SGV+ +G+ DQ+ VTGE ID+V L + +RKKF YA L+S+E V + + E
Sbjct: 319 SGVQSLAFEGETSDQLVVTGEGIDAVFLTNRMRKKFSYATLLSVEEVKEEKDEGGQDGGE 498
Query: 70 KKDKEKAEAIVAWPYVYSSVPHYPVY-EIKNEP---SCSIM 106
++D+ + P H P+Y + +P SCSI+
Sbjct: 499 EEDETETNENYPPPCPMCGTYHSPMYHHVVYDPYPNSCSIL 621
>TC215036 similar to PIR|T06397|T06397 isoprenylated protein FP6 - soybean
(fragment) {Glycine max;} , partial (28%)
Length = 639
Score = 47.0 bits (110), Expect = 1e-06
Identities = 33/98 (33%), Positives = 52/98 (52%), Gaps = 6/98 (6%)
Frame = +1
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVS 60
++ +++ KAM A G+SGVE ++ D KD + +ID V S LR K + ++VS
Sbjct: 115 LHGDRIKQKAMKTASGLSGVESVSV--DMKDMKMIVLGDIDPVSAVSKLR-KCCHTEIVS 285
Query: 61 IEAVGKTEEKKDKEKAEAIV------AWPYVYSSVPHY 92
+ K E+K++ E A+ V A+P Y PHY
Sbjct: 286 V-GPAKEEKKENVEPAKVPVPLKLHEAYPLYYQMTPHY 396
>BQ298167 weakly similar to GP|5732079|gb|A T32N4.11 gene product
{Arabidopsis thaliana}, partial (32%)
Length = 421
Score = 46.6 bits (109), Expect = 2e-06
Identities = 21/49 (42%), Positives = 34/49 (68%), Gaps = 1/49 (2%)
Frame = +3
Query: 19 GVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVS-IEAVGK 66
GV I+G++KD++ V GE++D+ L S +RKK G+ +LV+ +E GK
Sbjct: 150 GVNSVNIEGESKDRVVVIGEDVDAANLTSCMRKKVGHTELVTVVELKGK 296
>TC207614
Length = 801
Score = 44.7 bits (104), Expect = 7e-06
Identities = 29/80 (36%), Positives = 48/80 (59%), Gaps = 3/80 (3%)
Frame = +3
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVS 60
++ K++ KAM + G+SGVE ++ D KD+ +ID VK+A+ LR K +A++VS
Sbjct: 144 VHEDKIKQKAMKVVSGISGVESVSV--DMKDKKLTVIGDIDPVKVAAKLR-KLCHAEIVS 314
Query: 61 I---EAVGKTEEKKDKEKAE 77
+ + K E KKD +K +
Sbjct: 315 VGPAKEEKKEEPKKDDKKED 374
>TC217038
Length = 675
Score = 42.7 bits (99), Expect = 3e-05
Identities = 32/104 (30%), Positives = 55/104 (52%), Gaps = 9/104 (8%)
Frame = +3
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVS 60
+++ +++ KAM A G+SGV+ ++ D D+ ++D V S LR K+ + ++VS
Sbjct: 126 LHDDRIKRKAMKTASGLSGVQSVSV--DINDKKMTLLGDVDPVSAVSKLR-KWCHTEIVS 296
Query: 61 IEAVGKTEEKKDKEKAEAIV---------AWPYVYSSVPHYPVY 95
VG +E+K+KEK E A+P+ Y P P+Y
Sbjct: 297 ---VGPAKEEKEKEKVEPAKEPVPLKLYDAYPFYYHMTP--PLY 413
>TC228547
Length = 757
Score = 40.0 bits (92), Expect = 2e-04
Identities = 27/80 (33%), Positives = 43/80 (53%), Gaps = 3/80 (3%)
Frame = +1
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVS 60
+++ K + KAM G+SGVE T+ D D +D+V + LRK +AD++S
Sbjct: 109 IHDDKTKKKAMRAVSGISGVE--TVSVDMNDLKMTIIGNVDAVIVVGKLRKCCDHADILS 282
Query: 61 I---EAVGKTEEKKDKEKAE 77
+ + K E KKD++K E
Sbjct: 283 VGPAKEEKKEEPKKDEKKPE 342
>TC228803 PIR|T06397|T06397 isoprenylated protein FP6 - soybean (fragment)
{Glycine max;} , complete
Length = 644
Score = 39.7 bits (91), Expect = 2e-04
Identities = 26/85 (30%), Positives = 45/85 (52%), Gaps = 7/85 (8%)
Frame = +1
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRK-------KF 53
+++ K++ KAM G+SGVE ++ D KDQ ++D V + K KF
Sbjct: 115 LHDDKIKKKAMKAVSGISGVESVSV--DMKDQKMTIIGDVDPVTVVGKFTKFSLGKLRKF 288
Query: 54 GYADLVSIEAVGKTEEKKDKEKAEA 78
+ +++S+ K E KK+++K EA
Sbjct: 289 CHVEILSV-GPAKEEPKKEEKKPEA 360
>CF922268
Length = 556
Score = 32.7 bits (73), Expect = 0.028
Identities = 30/104 (28%), Positives = 47/104 (44%), Gaps = 4/104 (3%)
Frame = +1
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVS 60
+++ K++ KAM + +SGVE ++ D KDQ +ID V LRK
Sbjct: 103 LHDGKIKKKAMKVVSNLSGVESVSM--DMKDQKLTLIGDIDPVVAVEKLRKLCD----TR 264
Query: 61 IEAVGKTEEKKDKEKAEAIVA----WPYVYSSVPHYPVYEIKNE 100
I +VG +E+ + + E A + SV Y Y I N+
Sbjct: 265 IVSVGPAKEENEGKNNEVEAAENQNQNNLADSVNIYEAYHIHNQ 396
>TC207738 similar to UP|Q9FFE6 (Q9FFE6) AMP-binding protein (Adenosine
monophosphate binding protein 5 AMPBP5), partial (24%)
Length = 622
Score = 26.9 bits (58), Expect = 1.5
Identities = 27/96 (28%), Positives = 40/96 (41%), Gaps = 16/96 (16%)
Frame = +1
Query: 15 VGVSGVEG-TTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEK--- 70
VGV +G IK +KD I GE + SV++ S+L +G+ + V + E
Sbjct: 37 VGVMHEDGYLEIKDRSKDVIISGGENLSSVEVESVL---YGHPAVNEAAVVARPHEYWGE 207
Query: 71 ------------KDKEKAEAIVAWPYVYSSVPHYPV 94
K+KEK Y ++PHY V
Sbjct: 208 TPCAFVSLKREIKEKEKPTEKEIIEYCRDNMPHYMV 315
>TC226625 similar to UP|THIF_PEA (P29450) Thioredoxin F-type, chloroplast
precursor (TRX-F), partial (85%)
Length = 1128
Score = 26.2 bits (56), Expect = 2.6
Identities = 14/36 (38%), Positives = 20/36 (54%)
Frame = +3
Query: 8 NKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSV 43
N+ +AI +G+ V I DNK EVTG + D +
Sbjct: 822 NRPLAIELGIKVVPTFKILKDNKVVKEVTGAKYDDL 929
>TC216284 similar to GB|AAP68253.1|31711794|BT008814 At3g07950 {Arabidopsis
thaliana;} , partial (57%)
Length = 667
Score = 25.4 bits (54), Expect = 4.4
Identities = 12/41 (29%), Positives = 19/41 (46%), Gaps = 9/41 (21%)
Frame = -2
Query: 73 KEKAEAIVAWPYVYSSVP---------HYPVYEIKNEPSCS 104
K+K + PY + +P HYP+Y + N SC+
Sbjct: 423 KDKFKEFPGAPYWFKQLPNKQET*CADHYPIYRLFNISSCN 301
>CO985244
Length = 760
Score = 25.4 bits (54), Expect = 4.4
Identities = 24/78 (30%), Positives = 32/78 (40%), Gaps = 6/78 (7%)
Frame = -1
Query: 25 IKGDNKDQIEVTGE------EIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAEA 78
I G K+ IE E +++ L SL Y VS AVGK E K+ +
Sbjct: 544 IAGRPKEAIETFKEMVRASIQVNDCSLRSLGNLLLRYG--VSRLAVGKLEALVKKDASNG 371
Query: 79 IVAWPYVYSSVPHYPVYE 96
+ AW SSV Y+
Sbjct: 370 LQAWMLALSSVLEVDDYD 317
>AI794703
Length = 453
Score = 25.0 bits (53), Expect = 5.8
Identities = 9/22 (40%), Positives = 15/22 (67%)
Frame = +3
Query: 77 EAIVAWPYVYSSVPHYPVYEIK 98
E++V WP ++ VPH +EI+
Sbjct: 348 ESMVRWP*IHHPVPHQTWWEIQ 413
>TC218290 similar to UP|Q93VG8 (Q93VG8) AT4g17486/AT4g17486, partial (68%)
Length = 1333
Score = 25.0 bits (53), Expect = 5.8
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -1
Query: 48 LLRKKFGYADLVSIEAVGKTEEKKDKEKAEA 78
+LRKK G A + IE+V EKK K A
Sbjct: 184 MLRKKMGKA*I*RIESVDPKNEKKKKSGMSA 92
>TC224304 UP|Q7PWX9 (Q7PWX9) EbiP4168 (Fragment), partial (25%)
Length = 475
Score = 24.6 bits (52), Expect = 7.6
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +2
Query: 79 IVAWPYVYSSVPHYPVY 95
I + Y+Y +PHYP Y
Sbjct: 170 IYIYIYIYIYIPHYPFY 220
>BU760700
Length = 445
Score = 24.3 bits (51), Expect = 9.9
Identities = 12/51 (23%), Positives = 25/51 (48%)
Frame = +3
Query: 35 VTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAEAIVAWPYV 85
+T +++ ++ LA LRK+ G+ + G E K + + WP++
Sbjct: 78 LTADDMSAI-LAVKLRKRLGFGMWAKLHITGMHVEGKVLVGVKFLPTWPFI 227
>TC227271 similar to GB|AAG24276.1|10880493|AF195889 fasciclin-like
arabinogalactan protein FLA8 {Arabidopsis thaliana;} ,
partial (75%)
Length = 1213
Score = 24.3 bits (51), Expect = 9.9
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -1
Query: 18 SGVEGTTIKGDNKDQIEVTG 37
SGVE I GD QIE++G
Sbjct: 565 SGVERKGISGDGNGQIELSG 506
>TC231687 weakly similar to UP|Q84QE3 (Q84QE3) Avr9/Cf-9 rapidly elicited
protein 126, partial (8%)
Length = 808
Score = 24.3 bits (51), Expect = 9.9
Identities = 18/59 (30%), Positives = 27/59 (45%)
Frame = +2
Query: 12 AIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEK 70
AIA VS + G +NK EV G + S+KL + L S ++ K E++
Sbjct: 296 AIAAAVSSIRGQGSSSENKGDGEVLGSGL-SLKLGE-------HPQLASSNSLNKNEKE 448
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.310 0.129 0.353
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,700,300
Number of Sequences: 63676
Number of extensions: 34663
Number of successful extensions: 189
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of query: 106
length of database: 12,639,632
effective HSP length: 82
effective length of query: 24
effective length of database: 7,418,200
effective search space: 178036800
effective search space used: 178036800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 51 (24.3 bits)
Medicago: description of AC144618.2