BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144609.1 + phase: 0
(269 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC214700 homologue to UP|Q94FT5 (Q94FT5) Pectate lyase (Fragment... 244 2e-65
TC206141 homologue to UP|Q6U7H9 (Q6U7H9) Pectate lyase, partial ... 205 2e-53
TC228159 similar to UP|Q94FT5 (Q94FT5) Pectate lyase (Fragment),... 195 2e-50
TC208503 similar to UP|Q6U7H9 (Q6U7H9) Pectate lyase, partial (56%) 146 8e-36
BF426188 homologue to GP|11072028|gb F12A21.12 {Arabidopsis thal... 134 4e-32
BM527695 117 4e-27
BE806065 similar to GP|21537059|gb pectate lyase-like protein {A... 117 5e-27
TC234802 similar to GB|AAM61400.1|21537059|AY084835 pectate lyas... 84 7e-17
TC231233 similar to GB|BAA95715.1|7939512|AB024028 pectate lyase... 80 1e-15
TC222071 similar to UP|Q40319 (Q40319) Pectate lyase homolog , p... 75 2e-14
TC229412 similar to GB|AAM19958.1|20466073|AY098948 At5g04300/At... 65 3e-11
TC224238 similar to UP|Q40319 (Q40319) Pectate lyase homolog , p... 60 8e-10
BI974993 similar to PIR|T47653|T47 pectate lyase-like protein - ... 55 4e-08
AW570493 50 1e-06
TC221287 similar to UP|Q9M3D5 (Q9M3D5) Pectate lyase-like protei... 46 2e-05
TC222095 similar to UP|Q9M3D5 (Q9M3D5) Pectate lyase-like protei... 42 4e-04
TC214699 similar to UP|Q94FT5 (Q94FT5) Pectate lyase (Fragment),... 41 6e-04
TC206142 similar to UP|Q6U7H9 (Q6U7H9) Pectate lyase, partial (26%) 38 0.005
TC206140 similar to UP|Q6U7H9 (Q6U7H9) Pectate lyase, partial (25%) 34 0.061
TC227305 similar to GB|AAB51752.1|1934730|ATU95036 germin-like p... 33 0.10
>TC214700 homologue to UP|Q94FT5 (Q94FT5) Pectate lyase (Fragment), complete
Length = 2119
Score = 244 bits (624), Expect = 2e-65
Identities = 123/232 (53%), Positives = 153/232 (65%), Gaps = 2/232 (0%)
Frame = +3
Query: 28 YKVTDP-SDHPLNSTPGTLRYGASKIQGK-VWITFKRNMNIKLVRPLLISSFTTIDGRGV 85
Y VTDP D P+N PGTLR+ IQ K +WI FKR+M I+L + L+++SF TIDGRGV
Sbjct: 513 YVVTDPRDDDPVNPKPGTLRHAV--IQDKPLWIVFKRDMVIQLKQELIMNSFKTIDGRGV 686
Query: 86 DVHIADNACLMIYKATNIIIHGIRVHHCRPQAPGMVMGPDGKIISLGQVDGDAIRLVSAS 145
+VHIA+ AC+ I TN+IIHG+ +H C+P MV DGDAI + +S
Sbjct: 687 NVHIANGACITIQFVTNVIIHGLHIHDCKPTGNAMVRSSPTHFGWRTMADGDAISIFGSS 866
Query: 146 KIWIDHNTLYDCQDGLLDVTRGSTDITISNNWFREQNKVMLLGHDDGFVRDKNMKVTVVY 205
IW+DHN+L C DGL+D GST ITISNN F N+V+LLGH D + RDK M+VT+ Y
Sbjct: 867 HIWVDHNSLSHCADGLVDAVMGSTAITISNNHFTHHNEVILLGHSDSYTRDKLMQVTIAY 1046
Query: 206 NYFGPNCHQRMPRIRHGYAHVANNMYMGWVQYAIGGSMEPSLKSQSNLFIAP 257
N+FG QRMPR RHGY HV NN Y W YAIGGS P++ SQ N + AP
Sbjct: 1047NHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYNAP 1202
>TC206141 homologue to UP|Q6U7H9 (Q6U7H9) Pectate lyase, partial (65%)
Length = 990
Score = 205 bits (522), Expect = 2e-53
Identities = 102/226 (45%), Positives = 135/226 (59%)
Frame = +3
Query: 22 RKDLIHYKVTDPSDHPLNSTPGTLRYGASKIQGKVWITFKRNMNIKLVRPLLISSFTTID 81
R I+ D D +N PGTLR+ + + +WI F R+M I+L LL++SF TID
Sbjct: 315 RDGKIYVVEDDGDDDAVNPKPGTLRHAVIQDE-PLWIIFARDMVIQLKEELLMNSFKTID 491
Query: 82 GRGVDVHIADNACLMIYKATNIIIHGIRVHHCRPQAPGMVMGPDGKIISLGQVDGDAIRL 141
GRG VH+A C+ I TN+IIHGI +H C+ MV DGD + +
Sbjct: 492 GRGASVHVAGGPCITIQYVTNVIIHGIHIHDCKQGGNAMVRDSPRHYGWRTVSDGDGVSI 671
Query: 142 VSASKIWIDHNTLYDCQDGLLDVTRGSTDITISNNWFREQNKVMLLGHDDGFVRDKNMKV 201
S +W+DH +L +C DGL+D GST ITISNN+ +KVMLLGH D + +DK+M+V
Sbjct: 672 FGGSHVWVDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKDMQV 851
Query: 202 TVVYNYFGPNCHQRMPRIRHGYAHVANNMYMGWVQYAIGGSMEPSL 247
T+ +N+FG QRMPR R GY HV NN Y W YAIGGS P++
Sbjct: 852 TIAFNHFGEGLVQRMPRCRLGYFHVVNNDYTHWEMYAIGGSANPTI 989
>TC228159 similar to UP|Q94FT5 (Q94FT5) Pectate lyase (Fragment), partial
(68%)
Length = 1365
Score = 195 bits (496), Expect = 2e-50
Identities = 92/174 (52%), Positives = 114/174 (64%)
Frame = +2
Query: 85 VDVHIADNACLMIYKATNIIIHGIRVHHCRPQAPGMVMGPDGKIISLGQVDGDAIRLVSA 144
V+VHIA AC+ I TN+IIHG+ +H C+ MV DGD I + +
Sbjct: 2 VNVHIAYGACITIQFVTNVIIHGLHIHDCKVTGNAMVRSSPSHYGWRTLADGDGISIFGS 181
Query: 145 SKIWIDHNTLYDCQDGLLDVTRGSTDITISNNWFREQNKVMLLGHDDGFVRDKNMKVTVV 204
S IWIDHN+L +C DGL+D GST ITISNN+F N+VMLLGH D +VRDK M+VT+
Sbjct: 182 SHIWIDHNSLSNCADGLVDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIA 361
Query: 205 YNYFGPNCHQRMPRIRHGYAHVANNMYMGWVQYAIGGSMEPSLKSQSNLFIAPV 258
YN+FG QRMPR RHGY HV NN Y WV YAIGGS P++ SQ N ++AP+
Sbjct: 362 YNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRYLAPL 523
>TC208503 similar to UP|Q6U7H9 (Q6U7H9) Pectate lyase, partial (56%)
Length = 808
Score = 146 bits (369), Expect = 8e-36
Identities = 76/165 (46%), Positives = 97/165 (58%), Gaps = 1/165 (0%)
Frame = +1
Query: 28 YKVTDPSDH-PLNSTPGTLRYGASKIQGKVWITFKRNMNIKLVRPLLISSFTTIDGRGVD 86
Y VTD D P+ PGTLRY + + +WI F R+M IKL L+++SF TIDGRG
Sbjct: 313 YVVTDSGDDDPVTPKPGTLRYAVIQDE-PLWIIFARDMVIKLKEELIMNSFKTIDGRGAS 489
Query: 87 VHIADNACLMIYKATNIIIHGIRVHHCRPQAPGMVMGPDGKIISLGQVDGDAIRLVSASK 146
VHIA C+ I TN+IIHGI +H C+ MV DGD + + S
Sbjct: 490 VHIAGGPCITIQYVTNVIIHGINIHDCKQGGNAMVRDSPRHYGWRTISDGDGVSIFGGSH 669
Query: 147 IWIDHNTLYDCQDGLLDVTRGSTDITISNNWFREQNKVMLLGHDD 191
+W+DH +L +C DGL+D GST ITISNN+ +KVMLLGH D
Sbjct: 670 VWVDHCSLSNCNDGLIDAIHGSTGITISNNYMTHHDKVMLLGHSD 804
>BF426188 homologue to GP|11072028|gb F12A21.12 {Arabidopsis thaliana},
partial (30%)
Length = 362
Score = 134 bits (337), Expect = 4e-32
Identities = 59/104 (56%), Positives = 72/104 (68%)
Frame = +1
Query: 135 DGDAIRLVSASKIWIDHNTLYDCQDGLLDVTRGSTDITISNNWFREQNKVMLLGHDDGFV 194
DGD + + S +W+DH +L +C+DGL+D GST ITISNN+ NKVMLLGH D F
Sbjct: 49 DGDGVSIFGGSHVWVDHCSLSNCRDGLIDAIHGSTGITISNNYLTHHNKVMLLGHSDTFT 228
Query: 195 RDKNMKVTVVYNYFGPNCHQRMPRIRHGYAHVANNMYMGWVQYA 238
RDKNM+VT+ +N+FG QRMPR RHGY HV NN Y W YA
Sbjct: 229 RDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWRMYA 360
>BM527695
Length = 421
Score = 117 bits (294), Expect = 4e-27
Identities = 64/140 (45%), Positives = 86/140 (60%)
Frame = +2
Query: 61 KRNMNIKLVRPLLISSFTTIDGRGVDVHIADNACLMIYKATNIIIHGIRVHHCRPQAPGM 120
+R+M IKL + L+ISS TIDGRG +V D A L I N+IIHG+R+ + P+ GM
Sbjct: 2 QRSMMIKLNQELMISSDKTIDGRGANVVFRDGAGLTIQFVNNVIIHGVRIKNIVPKEGGM 181
Query: 121 VMGPDGKIISLGQVDGDAIRLVSASKIWIDHNTLYDCQDGLLDVTRGSTDITISNNWFRE 180
+ + + DGDAI + AS +WIDH +L +C DGL+DV +GST ITISN +
Sbjct: 182 IRDSYNHVGLRTKSDGDAISIFGASNVWIDHVSLSNCADGLIDVIQGSTAITISNCHMTK 361
Query: 181 QNKVMLLGHDDGFVRDKNMK 200
N VML G D + DK M+
Sbjct: 362 HNDVMLFGASDSYSGDKIMQ 421
>BE806065 similar to GP|21537059|gb pectate lyase-like protein {Arabidopsis
thaliana}, partial (28%)
Length = 414
Score = 117 bits (293), Expect = 5e-27
Identities = 60/136 (44%), Positives = 78/136 (57%), Gaps = 1/136 (0%)
Frame = +1
Query: 28 YKVTDPSDH-PLNSTPGTLRYGASKIQGKVWITFKRNMNIKLVRPLLISSFTTIDGRGVD 86
Y VTD SD P+N PGTLRY + + +WI F NM IKL + L+ +S+ TIDGRG D
Sbjct: 10 YIVTDSSDEDPVNPKPGTLRYAVIQNE-PLWIVFPSNMMIKLSQELIFNSYKTIDGRGAD 186
Query: 87 VHIADNACLMIYKATNIIIHGIRVHHCRPQAPGMVMGPDGKIISLGQVDGDAIRLVSASK 146
VHI C+ + +N+IIH I +HHC P V + DGD I + +
Sbjct: 187 VHIVGGGCITLQYISNVIIHNIHIHHCHPSGNANVRSSPEHYGYRTESDGDGISIFGSRD 366
Query: 147 IWIDHNTLYDCQDGLL 162
IWIDH TL C+DGL+
Sbjct: 367 IWIDHCTLSRCKDGLI 414
>TC234802 similar to GB|AAM61400.1|21537059|AY084835 pectate lyase-like
protein {Arabidopsis thaliana;} , partial (30%)
Length = 554
Score = 84.0 bits (206), Expect = 7e-17
Identities = 40/89 (44%), Positives = 55/89 (60%), Gaps = 1/89 (1%)
Frame = +3
Query: 28 YKVTDPSDH-PLNSTPGTLRYGASKIQGKVWITFKRNMNIKLVRPLLISSFTTIDGRGVD 86
Y VTD SD P N PGTLR+ + + +WI F +M I L L+ +S+ T+DGRG +
Sbjct: 246 YIVTDSSDRDPANPVPGTLRHAVIQDE-PLWIVFAADMTINLKHELIFNSYKTLDGRGAN 422
Query: 87 VHIADNACLMIYKATNIIIHGIRVHHCRP 115
VH+ + C+ + +NIIIH I VHHC P
Sbjct: 423 VHVTGHGCITLQYVSNIIIHNIHVHHCTP 509
>TC231233 similar to GB|BAA95715.1|7939512|AB024028 pectate lyase-like
protein {Arabidopsis thaliana;} , partial (34%)
Length = 614
Score = 79.7 bits (195), Expect = 1e-15
Identities = 37/69 (53%), Positives = 47/69 (67%), Gaps = 1/69 (1%)
Frame = +2
Query: 197 KNMKVTVVYNYFGPNCHQRMPRIRHGYAHVANNMYMGWVQYAIGGSMEPSLKSQSNLFIA 256
++M+VT+ +N+FG QRMPR RHGY HV NN Y W YAIGGS P++ SQ N F+A
Sbjct: 5 EDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLA 184
Query: 257 P-VTGKKEV 264
P + KEV
Sbjct: 185 PNIRFSKEV 211
>TC222071 similar to UP|Q40319 (Q40319) Pectate lyase homolog , partial (34%)
Length = 609
Score = 75.5 bits (184), Expect = 2e-14
Identities = 46/113 (40%), Positives = 63/113 (55%), Gaps = 1/113 (0%)
Frame = +3
Query: 28 YKVTDPSDHPL-NSTPGTLRYGASKIQGKVWITFKRNMNIKLVRPLLISSFTTIDGRGVD 86
Y VTD SD + N PGT+R+ ++ +G +WI F +M I L + L+ISS TIDGRG +
Sbjct: 273 YVVTDNSDDDMVNPKPGTIRHAVTQ-KGPLWIIFGHSMIISLRQELMISSDKTIDGRGAN 449
Query: 87 VHIADNACLMIYKATNIIIHGIRVHHCRPQAPGMVMGPDGKIISLGQVDGDAI 139
V A L I N+IIHG+R+ P+ GM+ + DGDAI
Sbjct: 450 VQFRGGAGLTIQFVNNVIIHGVRIKDIVPKDGGMIRDSADHYGLRTRSDGDAI 608
>TC229412 similar to GB|AAM19958.1|20466073|AY098948 At5g04300/At5g04300
{Arabidopsis thaliana;} , partial (37%)
Length = 925
Score = 65.1 bits (157), Expect = 3e-11
Identities = 28/55 (50%), Positives = 35/55 (62%)
Frame = +2
Query: 203 VVYNYFGPNCHQRMPRIRHGYAHVANNMYMGWVQYAIGGSMEPSLKSQSNLFIAP 257
+ +N+FG QRMPR R GY HV NN + W YAIGGS P++ SQ N + AP
Sbjct: 8 IAFNHFGEGLVQRMPRCRLGYIHVVNNDFTQWKMYAIGGSANPTINSQGNRYTAP 172
>TC224238 similar to UP|Q40319 (Q40319) Pectate lyase homolog , partial (27%)
Length = 512
Score = 60.5 bits (145), Expect = 8e-10
Identities = 28/52 (53%), Positives = 34/52 (64%)
Frame = +1
Query: 214 QRMPRIRHGYAHVANNMYMGWVQYAIGGSMEPSLKSQSNLFIAPVTGKKEVI 265
QRMPR R G+ HV NN Y W+ YAIGGS P++ SQ N FIAP ++I
Sbjct: 4 QRMPRCRWGFVHVLNNDYTHWMMYAIGGSSGPTILSQGNRFIAPNNNAAKLI 159
>BI974993 similar to PIR|T47653|T47 pectate lyase-like protein - Arabidopsis
thaliana, partial (41%)
Length = 424
Score = 54.7 bits (130), Expect = 4e-08
Identities = 49/139 (35%), Positives = 67/139 (47%)
Frame = +3
Query: 28 YKVTDPSDHPLNSTPGTLRYGASKIQGKVWITFKRNMNIKLVRPLLISSFTTIDGRGVDV 87
Y VT SD PG+LR G + + +WI F+ + I L L +SS+ TIDGRG V
Sbjct: 72 YFVTTLSD----DGPGSLREGCRRKE-PLWIVFEVSGTIHLSSYLSVSSYKTIDGRGQRV 236
Query: 88 HIADNACLMIYKATNIIIHGIRVHHCRPQAPGMVMGPDGKIISLGQVDGDAIRLVSASKI 147
+ L + + +III + R G D VDG I+ ++ I
Sbjct: 237 KLTGKG-LRLKECEHIIICNLEFEGGR--------GHD--------VDGIQIK-PNSRHI 362
Query: 148 WIDHNTLYDCQDGLLDVTR 166
WID TL D DGL+D+TR
Sbjct: 363 WIDRCTLRDYDDGLIDITR 419
>AW570493
Length = 103
Score = 49.7 bits (117), Expect = 1e-06
Identities = 20/34 (58%), Positives = 26/34 (75%)
Frame = +2
Query: 185 MLLGHDDGFVRDKNMKVTVVYNYFGPNCHQRMPR 218
MLLGH D + +DKNM+VT+ +N+FG QRMPR
Sbjct: 2 MLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPR 103
>TC221287 similar to UP|Q9M3D5 (Q9M3D5) Pectate lyase-like protein, partial
(44%)
Length = 540
Score = 46.2 bits (108), Expect = 2e-05
Identities = 26/71 (36%), Positives = 40/71 (55%)
Frame = +1
Query: 194 VRDKNMKVTVVYNYFGPNCHQRMPRIRHGYAHVANNMYMGWVQYAIGGSMEPSLKSQSNL 253
V D+ M+VT+ + +F QR PR+R H+ NN W YA+ S+E + SQ N+
Sbjct: 13 VGDRCMRVTIHHCFFN-GTRQRQPRVRFAKVHLYNNYIRNWGIYAVCASVESQIFSQHNI 189
Query: 254 FIAPVTGKKEV 264
+ A G+K+V
Sbjct: 190 YEA---GQKKV 213
>TC222095 similar to UP|Q9M3D5 (Q9M3D5) Pectate lyase-like protein, partial
(39%)
Length = 729
Score = 41.6 bits (96), Expect = 4e-04
Identities = 21/61 (34%), Positives = 33/61 (53%)
Frame = +2
Query: 196 DKNMKVTVVYNYFGPNCHQRMPRIRHGYAHVANNMYMGWVQYAIGGSMEPSLKSQSNLFI 255
D+ ++VT+ +F R PR+R G H+ NN W YA+ S+E + SQ N++
Sbjct: 2 DRCIRVTIHXXFFD-GTRXRQPRVRFGKVHLYNNYTRNWGIYAVCASVESQIYSQCNVYE 178
Query: 256 A 256
A
Sbjct: 179 A 181
>TC214699 similar to UP|Q94FT5 (Q94FT5) Pectate lyase (Fragment), partial
(28%)
Length = 1143
Score = 40.8 bits (94), Expect = 6e-04
Identities = 18/36 (50%), Positives = 22/36 (61%)
Frame = +2
Query: 222 GYAHVANNMYMGWVQYAIGGSMEPSLKSQSNLFIAP 257
GY HV N+ Y W YAI GS P++ SQ N + AP
Sbjct: 8 GYFHVLNHDYTHWEMYAIVGSANPTINSQGNRYHAP 115
>TC206142 similar to UP|Q6U7H9 (Q6U7H9) Pectate lyase, partial (26%)
Length = 723
Score = 37.7 bits (86), Expect = 0.005
Identities = 15/27 (55%), Positives = 18/27 (66%)
Frame = +3
Query: 231 YMGWVQYAIGGSMEPSLKSQSNLFIAP 257
Y W YAIGGS P++ SQ N F+AP
Sbjct: 18 YTHWEMYAIGGSANPTINSQGNRFVAP 98
>TC206140 similar to UP|Q6U7H9 (Q6U7H9) Pectate lyase, partial (25%)
Length = 683
Score = 34.3 bits (77), Expect = 0.061
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +2
Query: 234 WVQYAIGGSMEPSLKSQSNLFIAP 257
W YA GGS P++ SQ N F+AP
Sbjct: 2 WEMYAXGGSANPTINSQGNRFVAP 73
>TC227305 similar to GB|AAB51752.1|1934730|ATU95036 germin-like protein
{Arabidopsis thaliana;} , partial (96%)
Length = 1034
Score = 33.5 bits (75), Expect = 0.10
Identities = 18/49 (36%), Positives = 25/49 (50%)
Frame = +1
Query: 32 DPSDHPLNSTPGTLRYGASKIQGKVWITFKRNMNIKLVRPLLISSFTTI 80
D S P+NS+ LR S KVW T +R M I L+ L S+ ++
Sbjct: 490 DSSPQPINSSQSQLRRVKSLCSQKVWCTIRRTMGISLLLCFLPSTANSL 636
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.138 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,809,695
Number of Sequences: 63676
Number of extensions: 174494
Number of successful extensions: 754
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 743
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 745
length of query: 269
length of database: 12,639,632
effective HSP length: 96
effective length of query: 173
effective length of database: 6,526,736
effective search space: 1129125328
effective search space used: 1129125328
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC144609.1